Department of Biochemistry
Bose Institute
Centenary Campus
P 1/12, C. I. T. Road, Scheme - VIIM
Kolkata - 700054
West Bengal, India
 | abhrajyoti.ghosh@jcbose.ac.in |
Abhrajyoti Ghosh, M.Sc., PhD
Assistant Professor
Department of Biochemistry
Bose Institute
Centenary Campus
P 1/12, C. I. T. Road, Scheme - VIIM
Kolkata - 700054
West Bengal, India
Phone: +91-33-25693225 (Off)
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Professional Experience
Ramanujan Fellow of Department of Biochemistry, Bose Institute, Kolkata, India (2014- )
Senior Research Associate in Department of Biochemistry, University of Calcutta, India (2012-2013)
Postdoctoral Fellow in the Junior Independent Group of ‘Molecular Biology of Archaea’ headed by Dr. Sonja-Verena Albers, in Max Planck Institute for Terrestrial Microbiology, Marburg, Germany (2008-2012)
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Education
2003-2008
PhD in Biochemistry, University of Calcutta, India
Thesis Title: “Characterization and cloning of keratinolytic protease from Bacillus cereus DCUW isolated from a functional screening based study on East Calcutta Wetland Area” Under supervision of Prof. Dhrubajyoti Chattopadhyay, Department of Biochemistry University of Calcutta, India.
2001-2003
M.Sc in Biochemistry, University of Calcutta, India
1998-2001
B.Sc (Hons) in Chemistry, University of Calcutta, India
Subsidiary subjects-Physics, Mathematics and Environmental Sciences
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Research Interest:
Comparative and functional genomics of bacterial and archaeal pathways, bacterial and archaeal type IV-pili like surface appendages, Sec secretion pathway in archaea, Signal recognition particle (SRP) in archaeal system, bacterial and archaeal diversity in marine sediments, bioleaching of metals and sulfur oxidation in extremophiles.
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Research Experience:
In my PhD study I have demonstrated the microbial diversity in East Calcutta Wetland (ECW) area using both functional screening based and metagenomic microbial analysis. In spite of tremendous anthropogenic stress, ECW soil contains large number of different microbial populations. Detail study on soil microbial structure and their function resulted in successful isolation of 38 pure bacterial strains of biotechnological interest. Structural metagenomic analysis revealed the microbial composition at greater extent including both cultivable and non-cultivable bacterial populations. The other major work in my thesis was on isolation and characterization of a high molecular weight feather degrading protease from Bacillus cereus W (an ECW isolate). The enzyme was purified, characterized and identified as minor extra-cellular protease (Vpr). Based on the domain architecture, feather degrading Vpr was further characterized using four truncated form of the wild type protein (heterologously expressed in E.coli). The result showed that the maturation of the Vpr needed both N-terminal and C-terminal processing. The oligomerization property was associated with the C-terminal protease-associated domain, which also found to be responsible for the substrate-binding specificity of Vpr.
I started as a postdoctoral researcher in ‘Junior Independent Max Planck Research Group’ headed by Dr. Sonja-Verena Albers at MPI, Marburg, Germany in Nov’08. Here I started working with flagella system of S. acidocaldarius, a hyperthermophilic crenarchaea. I have characterized the secretion system ATPase, FlaI (ATPase in flagella system) and also solved the crystal structure of this enzyme in collaboration with Dr. Sophia Hartung at Lawrence Berkeley National Laboratory, USA. Combining in vivo and structural information, we could successfully demonstrate that FlaI is a dual function ATPase that not only empowers assembly of archaeal flagella but also provides energy for its rotation. Besides I am also working on PibD, a class III signal peptidase which processes the pre-flagellin (FlaB) in Sulfolobales.
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Assistant Professor
Department of Biochemistry
Bose Institute
Centenary Campus
P 1/12, C. I. T. Road, Scheme - VIIM
Kolkata - 700054
West Bengal, India
Phone: +91-33-25693225 (Off)
------------------------------------------
Professional Experience
Ramanujan Fellow of Department of Biochemistry, Bose Institute, Kolkata, India (2014- )
Senior Research Associate in Department of Biochemistry, University of Calcutta, India (2012-2013)
Postdoctoral Fellow in the Junior Independent Group of ‘Molecular Biology of Archaea’ headed by Dr. Sonja-Verena Albers, in Max Planck Institute for Terrestrial Microbiology, Marburg, Germany (2008-2012)
----------------------
----------------------
Education
2003-2008
PhD in Biochemistry, University of Calcutta, India
Thesis Title: “Characterization and cloning of keratinolytic protease from Bacillus cereus DCUW isolated from a functional screening based study on East Calcutta Wetland Area” Under supervision of Prof. Dhrubajyoti Chattopadhyay, Department of Biochemistry University of Calcutta, India.
2001-2003
M.Sc in Biochemistry, University of Calcutta, India
1998-2001
B.Sc (Hons) in Chemistry, University of Calcutta, India
Subsidiary subjects-Physics, Mathematics and Environmental Sciences
-------------------
-------------------
Research Interest:
Comparative and functional genomics of bacterial and archaeal pathways, bacterial and archaeal type IV-pili like surface appendages, Sec secretion pathway in archaea, Signal recognition particle (SRP) in archaeal system, bacterial and archaeal diversity in marine sediments, bioleaching of metals and sulfur oxidation in extremophiles.
--------------------
--------------------
Research Experience:
In my PhD study I have demonstrated the microbial diversity in East Calcutta Wetland (ECW) area using both functional screening based and metagenomic microbial analysis. In spite of tremendous anthropogenic stress, ECW soil contains large number of different microbial populations. Detail study on soil microbial structure and their function resulted in successful isolation of 38 pure bacterial strains of biotechnological interest. Structural metagenomic analysis revealed the microbial composition at greater extent including both cultivable and non-cultivable bacterial populations. The other major work in my thesis was on isolation and characterization of a high molecular weight feather degrading protease from Bacillus cereus W (an ECW isolate). The enzyme was purified, characterized and identified as minor extra-cellular protease (Vpr). Based on the domain architecture, feather degrading Vpr was further characterized using four truncated form of the wild type protein (heterologously expressed in E.coli). The result showed that the maturation of the Vpr needed both N-terminal and C-terminal processing. The oligomerization property was associated with the C-terminal protease-associated domain, which also found to be responsible for the substrate-binding specificity of Vpr.
I started as a postdoctoral researcher in ‘Junior Independent Max Planck Research Group’ headed by Dr. Sonja-Verena Albers at MPI, Marburg, Germany in Nov’08. Here I started working with flagella system of S. acidocaldarius, a hyperthermophilic crenarchaea. I have characterized the secretion system ATPase, FlaI (ATPase in flagella system) and also solved the crystal structure of this enzyme in collaboration with Dr. Sophia Hartung at Lawrence Berkeley National Laboratory, USA. Combining in vivo and structural information, we could successfully demonstrate that FlaI is a dual function ATPase that not only empowers assembly of archaeal flagella but also provides energy for its rotation. Besides I am also working on PibD, a class III signal peptidase which processes the pre-flagellin (FlaB) in Sulfolobales.
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