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César Guerrero


cesar.guerrero@umh.es

Journal articles

2011
Paulo Pereira, Xavier Ubeda, Deborah Martin, Jorge Mataix-Solera, César Guerrero (2011)  Effects of a low severity prescribed fire on water-soluble elements in ash from a cork oak (Quercus suber) forest located in the northeast of the Iberian Peninsula.   Environ Res 111: 2. 237-247 Feb  
Abstract: Wildfire is the major disturbance in Mediterranean forests. Prescribed fire can be an alternative to reduce the amount of fuel and hence decrease the wildfire risk. However the effects of prescribed fire must be studied, especially on ash properties, because ash is an important nutrient source for ecosystem recovery. The aim of this study is to determine the effects of a low severity prescribed fire on water-soluble elements in ash including pH, electrical conductivity (EC), calcium (Ca), magnesium (Mg), sodium (Na), potassium (K), aluminum (Al), manganese (Mn), iron (Fe), zinc (Zn), silica (SiO(2)) and total sulphur (TS). A prescribed fire was conducted in a cork oak (Quercus suber) (Q.S) forest located in the northeast part of the Iberian Peninsula. Samples were collected from a flat plot of 40Ã70m mainly composed of Q.S and Quercus robur (Q.R) trees. In order to understand the effects of the prescribed fire on the soluble elements in ash, we conducted our data analysis on three data groups: all samples, only Q.S samples and only Q.R samples. All three sample groups exhibited a significant increase in pH, EC (p<0.001), water-soluble Ca, Mg, Na, SiO(2) and TS and a decrease in water-soluble Mn, Fe and Zn. Differences were identified between oak species for water-soluble K, Al and Fe. In Q.S samples we registered a significant increase in the first two elements p<0.001 and p<0.01, respectively, and a non-significant impact in the third, at p<0.05. In Q.R data we identified a non-significant impact on water-soluble K and Al and a significant decrease in water-soluble Fe (p<0.05). These differences are probably due to vegetation characteristics and burn severity. The fire induced a higher variability in the ash soluble elements, especially in Q.S samples, that at some points burned with higher severity. The increase of pH, EC, Ca, Mg, Na and K will improve soil fertility, mainly in the study area where soils are acidic. The application of this low severity prescribed fire will improve soil nutrient status without causing soil degradation and thus is considered to be a good management strategy.
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2010
F García-Orenes, C Guerrero, A Roldán, J Mataix-Solera, A Cerdà, M Campoy, R Zornoza, G Bárcenas, F Caravaca (2010)  Soil microbial biomass and activity under different agricultural management systems in a semiarid Mediterranean agroecosystem   Soil and Tillage Research 109: 110-115  
Abstract: A field experiment was carried out in a semiarid agricultural Mediterranean area located at the âEl Teularetâ experimental field in the Enguera Sierra (Valencia, southeast Spain) to assess the influence of different agricultural management systems on indicators of soil biological quality and activity (microbial biomass C, basal respiration, C mineralization coefficients, metabolic quotient (qCO2), respiratory quotient (RQ: moles CO2 evolved/moles O2 consumed), soluble C and dehydrogenase, urease, protease-BAA, phosphatase and β-glucosidase activities), one year after treatment establishment. The management practices assayed were as follows: application of the herbicides paraquat, glyphosate or oxyfluorfen, addition of olive tree pruning residues, ploughing, sowing of oats + addition of crop residues + ploughing, sowing of Medicago sativa, sowing of oats and vetch + addition of crop residues and addition of oat straw. A non-treated plot was used as control soil and a plot under natural vegetation was used as a standard of local, high quality soil. The plots with addition of oat straw had higher values of enzymatic activity, microbial biomass and respiration, reaching similar values to soil under native vegetation. The lowest levels of soil biological quality indicators were observed in the plots with application of some type of herbicide. Low RQ values were observed in these plots as consequence of the scarce-null inputs of organic matter, suggesting an increase in organic matter recalcitrance. The addition of oat straw to soil can be considered an effective technology, due to the rapid improvement of soil quality, for carrying out sustainable agriculture in semiarid Mediterranean agroecosystems.
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C Guerrero, R Zornoza, I Gómez, J Mataix-Beneyto (2010)  Spiking of NIR regional models using samples from target sites: Effect of model size on prediction accuracy   Geoderma 158: 66-77  
Abstract: Near infrared reflectance (NIR) spectroscopy has many advantages because it is a rapid and cost-effective technique. A needed steep is the development of soil spectral libraries and models (calibrations using multivariate techniques). The calibrations should contain the variability of the target site soils on which the models are to be used. Many times this premise is not easy to fulfil. A classical way to solve this problem is by the spiking of models with a few samples from the target site (local samples), and the subsequent recalibration of models. In this regional-scale study we hypothesized that small-sized models should integrate the spectral characteristics of local samples more easily than large-sized models and as consequence should produce more accurate predictions. With the aim to test this hypothesis different-sized models were constructed using different quantities of soil samples. Partial least squares (PLS) regression was used to construct the models which were relating NIR spectra to the Nitrogen Kjeldahl (NKj) contents in soil samples. Two strategies were used for the selection of samples in models: (i) strategy based on spectra characteristics (SC) and (ii) strategy based on NKj values (NV) of the samples. These different-sized models were sequentially spiked with local samples from target sites and recalibrated. The predictions accuracies obtained with the models before and after spiking were studied being the NKj the soil property selected. In general all predictions were very accurate including those obtained before the spiking of models. Predictions accuracy increased as consequence of spiking in three of the four target sites studied. A negative trend was observed between prediction accuracy and model size. The lower errors were obtained using small-sized models after spiking which were more accurate than local models too. It was noticeable the high accuracy obtained by local models which were constructed using only 20 local samples. Before spiking SC models were more accurate than NV models but scarce differences between both strategies were observed after spiking with 20 samples. The results suggested that small-sized models can be useful for local predictions after spiking and they were also emphasizing the relevant role of local samples in models. The results obtained could encourage the expansion of this technique because large data based seem not be needed. Therefore NIR users could primarily focus most of their efforts on obtaining highly accurate analytical values in a few set of samples.
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A Pérez-Bejarano, J Mataix-Solera, R Zornoza, C Guerrero, V Arcenegui, J Mataix-Beneyto, S Cano-Amat (2010)  Influence of plant species on physical, chemical and biological soil properties in a Mediterranean forest soil   European Journal of Forest Research 129: 1. 15-24  
Abstract: In semiarid ecosystems plant cover plays an important role in the improvement of physical, chemical and biochemical soil properties. With the aim of studying the influence of different plant species on soil properties, and establishing the relationships between them, 160 soil samples from under four different plant species (Pinus halepensis, Quercus coccifera, Juniperus oxycedrus and Rosmarinus officinalis) were taken in a forest area of the province of Alicante (SE Spain). The following soil properties were analyzed in all soil samples: organic carbon content, microbial biomass, soluble organic carbon, aggregate stability, basal respiration, and some eco-physiological ratios. In addition, the near infrared spectra (NIR) of all soil samples were obtained to verify the similarities or differences between soil samples under the four species. Some differences in parameters such as organic carbon content or basal respiration were found mainly between the group of P. halepensis and Q. coccifera with respect to J. oxycedrus and R. officinalis. Despite this, the high organic carbon content found under the four plant species showed an influence on the rest of soil properties. Moreover, using a discriminant analysis with factorial scores from NIR absorbance data did not result in a good classification of samples in terms of the species, reflecting some similarities between them. Our results show that the high contents observed in some parameters under the four species, and the lack of significant differences in most of them, prove the important role of shrubland in semiarid conditions, it being capable of promoting good soil conditions.
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2009
R Zornoza, J Mataix-Solera, C Guerrero, V Arcenegui, J Mataix-Beneyto (2009)  Storage effects on various biochemical properties of air-dried soil samples from south-eastern Spain   Arid Land Research and Management 23: 213-222  
Abstract: Soil biochemical properties are susceptible to change under sample storage, and as a consequence, these properties have usually been determined in fresh samples, kept cold or frozen for brief periods of time. However, air-dried soil would facilitate routine soil testing procedures in soils from semi-arid Mediterranean areas, which have soil water deficit most of the year. This research aims at assessing the effects of medium-term soil storage (6-9 months) at room temperature on air-dried soil samples from two Mediterranean forest locations for the measurement of various microbiological and biochemical properties (microbial biomass carbon, basal respiration, metabolic quotient, acid phosphatase activity, urease activiy, β-glucosidase activity, and soluble carbon). Storage of air-dried soil samples for 6 months had no significant effects on the studied properties in any location. With regard to samples stored for 9 months, we only found differences at the location with higher mean rainfall in the values of basal respiration, the metabolic quotient and β-glucosidase, and urease activities. Our results show that biochemical properties from Mediterranean semi-arid soils, are medium-term stable in stored air-dried soil samples. Thus, these findings would encourage the selection of biochemical properties on a practical basis, as there is no strict requirement to determine these properties immediately after sampling, as they remain valid for several months.
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R Zornoza, C Guerrero, J Mataix-Solera, K M Scow, V Arcenegui, J Mataix-Beneyto (2009)  Changes in soil microbial community structure following the abandonment of agricultural terraces in mountainous areas of Eastern Spain   Applied Soil Ecology 42: 315-323  
Abstract: In Eastern Spain, almond trees have been cultivated in terraced orchards for centuries, forming an integral part of the Mediterranean forest scene. In the last decades, orchards have been abandoned due to changes in society. This study investigates effects of changes in land use from forest to agricultural land and the posterior land abandonment on soil microbial community, and the influence of soil physico-chemical properties on the microbial community composition (assessed as abundances of phospholipids fatty acids, PLFA). For this purpose, three land uses (forest, agricultural and abandoned agricultural) at four locations in SE Spain were selected. Multivariate analysis showed a substantial level of differentiation in microbial community structure according to land use. The microbial communities of forest soils were highly associated with soil organic matter content. However, we have not found any physical or chemical soil property capable of explaining the differences between agricultural and abandoned agricultural soils. Thus, it was suggested that the cessation of the perturbation caused by agriculture and shifts in vegetation may have led to changes in the microbial community structure. PLFAs indicative of fungi and ratio of fungal to bacterial PLFAs were higher in abandoned agricultural soils, whereas the relative abundance of bacteria was higher in agricultural soils. Actinomycetes were generally lower in abandoned agricultural soils, while the proportions of vesicularâarbuscular mycorrhyzal fungi were, as a general trend, higher in agricultural and abandoned agricultural soils than in forests. Total microbial biomass and richness increased as agricultural < abandoned agricultural < forest soils.
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R Zornoza, J Mataix-Solera, C Guerrero, V Arcenegui, J Mataix-Beneyto (2009)  Comparison of soil physical, chemical, and biochemical properties among native forest, maintained and abandoned almond orchards in mountainous areas of Eastern Spain   Arid Land Research and Management 23: 267-282  
Abstract: Abstract In the mountainous areas of Eastern Spain, soils have been cultivated in terraced orchards for centuries, although in the last decades, almond orchards are being abandoned. For this study, we selected four locations in SE Spain, with a similar vegetation mosaic, constituting forest, almond tree orchards, and orchards abandoned between 10 to 15 years previous to sampling. The main objective was to investigate the effects of changes in land use from forest to agricultural and posterior land abandonment on various physical, chemical, and biochemical properties. In all locations, all properties showed the highest values in forest soils, excepting pH and some eco-physiological ratios which were lowest under this land use. Abandoned agricultural soils showed a slight recovery in some properties compared to agricultural soils, the biochemical properties being the most sensitive indicators in reflecting these changes. All these results indicate that after land abandonment, soil microorganisms are more active as a consequence of the increment in the vegetation cover, with higher inputs as litter and root exudates. Moreover, the stopping of tillage may also have favored the increments in microbial biomass and activity. Nonetheless, these values are still low compared to forest soils, reflecting that 10-15 years of abandonment is not long enough to achieve a significant recovery in soil properties under semi-arid Mediterranean conditions. The metabolic quotient (qCO2) showed no general pattern in all locations in terms of land use, suggesting that this ratio is not specific enough to be used as an indicator in ecosystem succession.
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F García-Orenes, A Cerdà, J Mataix-Solera, C Guerrero, M B Bodí, V Arcenegui, R Zornoza, J G Sempere (2009)  Effects of agricultural management on surface soil properties and soil-water losses in eastern Spain   Soil & Tillage Research 106: 1. 117-123  
Abstract: In Spain, agriculture triggers soil degradation and erosion processes. New strategies have to be developed to reduce soil losses and recover or maintain soil functionality in order to achieve a sustainable agriculture. An experiment was designed to evaluate the effect of different agricultural management on soil properties and soil erosion. Five different treatments (ploughing, herbicide, control, straw mulch and chipped pruned branches) were established in âEl Teularet experimental stationâ located in the Sierra de Enguera (Valencia, Spain). Soil sampling was conducted prior to treatment establishment, and again after 16 months, to determine soil organic matter content (OM), aggregate stability (AS), and microbial biomass carbon content (Cmic). Fifty rainfall simulations tests (55 mm during one hour, 5-year return period) were applied to measure soil and water losses under each treatment. The highest values of OM, AS and Cmic were observed in the straw-covered plot, where soil and water losses were negligible. On the contrary, the plot treated with herbicides had the highest soil losses and a slight reduction in Cmic. Soil erosion control was effective after 16 months on the plots where vegetation was present while on the ploughed and herbicide-treated plots, the practices were not sustainable due to large water and soil losses. Except for the straw mulch plot, soil properties (OM, AS, Cmic) were not enhanced by the new land managements, but soil erosion control was achieved on three of the five plots used (weeds, weeds plus straw and weeds plus chipped pruned branches). Erosion control strategies such as weeds, weeds plus straw mulch and weeds plus chipped branches mulch are highly efficient in reducing soil losses on traditional herbicide-treated and ploughed agricultural land. However, it takes longer to recover other soil properties such as OM, AS, and Cmic.
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2008
J M Soriano-Disla, I Gomez, C Guerrero, M M Jordan, J Navarro-Pedreño (2008)  Soil factors related to heavy metal bioavailability after sewage sludge application   Fresenius Environmental Bulletin 17: 41. 1839-1845  
Abstract: Two main advantages are achieved with the application of sewage sludge as soil amendment: the right management of an unwanted waste product and the improvement of soil properties. On the other hand, the presence of heavy metals in the wastes may lead to risk for humans and the environment. The current legislation in Europe and Spain limits the application to the soil as a function of the total heavy metal content in sewage sludge and soil depending on soil pH. However, it would be necessary to use the hioavailable fraction and to include more soil properties. Thus, the objective of the present work was to study the influence of soil properties in the uptake of heavy metals by plants and the suitability of DTPA extraction as a method to predict this bioavailability. With these objectives. 36 agricultural soils were mixed with two types of sewage sludges having different levels of heavy metals and a bioassay with barley was carried out under greenhouse conditions. DTPA extraction was capable to predict the accumulation of heavy metals in roots but failed for the case of Cr. All the predictions were improved when some soil properties (texture, pH, organic matter and electrical conductivity) were taken into account using multiple regression. The regression coefficients for each metal were: Cd (r=0.922; p<0.001), Zn (r=0.894: p<0.001), Pb (r=0.846; p<0.001), Ni (r=0.828; p<0.001), Cu (=0.798; p<0.001), Cr (r=0.515; p<0.001). These results suggest the important role of other soil properties apart from pH on the availability of heavy metals. Moreover. the use of DTPA data together with soil properties could be an effective way to asses the availability of heavy metals to plants.
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V Arcenegui, C Guerrero, J Mataix-Solera, J Mataix-Beneyto, R Zornoza, J Morales, A M Mayoral (2008)  The presence of ash as an interference factor in the estimation of the maximum temperatures reached on burned soils using near-infrared spectroscopy (NIR)   Catena 74: 177-184  
Abstract: The aim of this work was to assess the effect of the presence of ash on maximum temperature reached (MTR) estimation using near infrared reflectance (NIR) spectroscopy. The degree of combustion (ash produced by heating to 100, 300, 500 and 700 °C), the type (ash from Pinus halepensis and Rosmarinus officinalis), and different quantities of ash (0â20% in 2% interval) were evaluated in a soil heated at seven different temperatures (100 °Câ700 °C). Results showed that the estimation of MTR on samples with ash, using partial least squares (PLS) models constructed with samples without ash, could be erroneous. Both, ash quantity and degree of combustion affected the estimation of MTR. However, using discriminant analysis, a good classification of samples (> 97% correctly classified) according to the heating temperature classes (unheated, 100, 200, 300, 400, 500, 600 and 700 °C) was obtained despite the presence of ash.
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R Zornoza, J Mataix-Solera, C Guerrero, V Arcenegui, J Mataix-Beneyto, I Gómez (2008)  Validating the effectiveness and sensitivity of two soil quality indices based in natural forest soils under Mediterranean conditions   Soil Biology & Biochemistry 40: 2079-2087  
Abstract: Soils from natural ecosystems have specific physical, chemical and biochemical properties determined by the conditions in which these soils have developed. These soils that develop without external disturbance reach a balance amongst their properties. Thus, the creation of a model that represents the established balance of different soil properties from stable ecosystems can be used as a soil quality index, thus any perturbation must lead to modifications in this natural balance. Two regression models with soils from undisturbed forest regions in eastern Spain were previously developed, representing the balance between organic carbon and some physical, chemical and biochemical properties. For undisturbed forest soils, the prediction of soil organic carbon (SOCc) with the calibrated models should be similar to the actual value of this variable (SOCa) (SOCc â SOCa). Consequently, the residuals (SOCc â SOCa) should be around 0. On the contrary, disturbance practices cause a disruption in the balance defined between the different properties and SOC. As a consequence, residuals must be < or >0. Furthermore, the more the degree of degradation increases, the more the values of SOCc must differ from the values of SOCa. According to this, two soil quality indices (SQI) were defined, one for each model, by the calculation of the model residuals (SQI = SOCc â SOCa). The SQIs have been applied to different undisturbed forest soils to evaluate their validity. In addition, they have also been applied to severely altered soils, like agricultural soils, and abandoned agricultural fields, to assess the sensibility of this index to perturbations. After applying the soil quality indices to eleven undisturbed forest soils, it has been verified that a balance exists between organic matter content and different physical, chemical and biochemical properties in forest soils from SE Spain, and the proposed calibrated models are capable of reflecting that balance (SQI â 0). Our results confirm that our models are sensitive to soil perturbation, because agricultural and abandoned agricultural soils have shown an imbalance between organic carbon content and the physical, chemical and biochemical properties (SQI > 0). Moreover, soils from abandoned fields showed lower deviation in the natural equilibrium, indicating a recovery of soil quality.
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R Zornoza, C Guerrero, J Mataix-Solera, K M Scow, V Arcenegui, J Mataix-Beneyto (2008)  Near infrared spectroscopy for determination of various physical, chemical and biochemical properties in Mediterranean soils   Soil Biology & Biochemistry 40: 1923-1930  
Abstract: The potential of near infrared (NIR) reflectance spectroscopy to predict various physical, chemical and biochemical properties in Mediterranean soils from SE Spain was evaluated. Soil samples (n = 393) were obtained by sampling 13 locations during three years (2003â2005 period). These samples had a wide range of soil characteristics due to variations in land use, vegetation cover and specific climatic conditions. Biochemical properties also included microbial biomarkers based on phospholipid fatty acids (PLFA). Partial least squares (PLS) regression with cross validation was used to establish relationships between the NIR spectra and the reference data from physical, chemical and biochemical analyses. Based on the values of coefficient of determination (r2) and the ratio of standard deviation of validation set to root mean square error of cross validation (RPD), predicted results were evaluated as excellent (r2 > 0.90 and RPD > 3) for soil organic carbon, Kjeldahl nitrogen, soil moisture, cation exchange capacity, microbial biomass carbon, basal soil respiration, acid phosphatase activity, β-glucosidase activity and PLFA biomarkers for total bacteria, Gram-positive bacteria, actinomycetes, vesicular-arbuscular mycorrhizal fungi and total PLFA biomass. Good predictions (0.81 < r2 < 0.90 and 2.5 < RPD < 3) were obtained for exchangeable calcium and magnesium, water soluble carbon, water holding capacity and urease activity. Resultant models for protozoa and fungi were not accurate enough to satisfactorily estimate these variables, only permitting approximate predictions (0.66 < r2 < 0.80 and 2.0 < RPD < 2.5). Electrical conductivity, pH, exchangeable phosphorus and sodium, metabolic quotient and Gram-negative bacteria were poorly predicted (r2 < 0.66 and RPD < 2). Thus, the results obtained in this study reflect that NIR reflectance spectroscopy could be used as a rapid, inexpensive and non-destructive technique to predict some physical, chemical and biochemical soil properties for Mediterranean soils, including variables related to the composition of the soil microbial community composition.
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J Mataix-Solera, V Arcenegui, C Guerrero, M Jordán, P Dlapa, N Tessler, L Wittenberg (2008)  Can terra rossa become water repellent by burning? A laboratory approach   Geoderma 147: 178-184  
Abstract: Fire usually induces water repellency (WR) in soils. Reduction in infiltration rates, increase of runoff and erosion are some of the consequences of WR in fire-affected soils. Most forest soils can develop WR by burning; however some previous observations in burned terra rossa soils have shown little changes in WR. Laboratory controlled experiments have been done with samples of terra rossa from 14 different sites. The objectives are to confirm whether the observed is a common behaviour of terra rossa and to explore the factors controlling the wettability of this soil type after burning. Samples from the upper 2.5 cm of terra rossa were collected from 12 forest sites of the Alicante province (Spain), and from 2 sites in the âMt. Carmelâ, Haifa (Israel) with similar environmental conditions. Laboratory burning of samples at 250 °C, 300 °C and 350 °C was performed with and without the addition of litter of Pinus halepensis. The results confirm that all soils have a very low susceptibility to become water repellent by burning. Without the addition of litter, WR was not detected in any soil sample at any temperature of burning. With the addition of litter, WR was present only in six of the soils after some of the heating treatments. Although all soils had enough soil organic matter (SOM) to develop WR by heating, the ratio between SOM and clay content was considerably lower compared to other types of forest soils of the region in which WR has been found after forest fires. This could explain in part the lower susceptibility of terra rossa to become water repellent by burning since, as some authors have indicated, fine-textured soils are less prone to develop soil WR due to their high specific surface area. From mineralogical analysis of the clay fraction we found that the dominant clay types in the studied terra rossa were kaolinite and illite, with the exception of one soil where Caâmontmorillonite content is higher than kaolinite and illite. Caâmontmorillonite was present in only three of the soils. Comparing the soil properties between the group of terra rossa that in no case become water repellent (wettables) with the group that in some cases developed WR (potentially water repellents), some differences were found: the kaolinite content is higher in the wettables group (P < 0.05), and the soils containing Caâmontmorillonite are in the group of potentially water repellents. A clear separation between the 2 groups was found when we compared SOM vs kaolinite contents, the kaolinite content being the main factor contributing to this separation. These results are in agreement with those obtained in experiments with clay additions to water repellent soils in order to reduce the WR, and also with some studies which found that kaolinite is one of the most effective clay minerals for this purpose.
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V Arcenegui, J Mataix-Solera, C Guerrero, R Zornoza, J Mataix-Beneyto, F García-Orenes (2008)  Immediate effects of wildfires on water repellency and aggregate stability in Mediterranean calcareous soils   Catena 74: 219-226  
Abstract: Alkaline soils are considered much less prone to developing water repellency induced by fire than acidic soils. Here we report on the persistence of water repellency present in calcareous soils immediately after wildfires in 10 burned areas in SE Spain, its distribution in different aggregate size fractions (< 2, 2â1, 1â0.5, 0.5â0.25 and < 0.25 mm) and on results from aggregate stability tests. We also distinguished between soil samples taken beneath pine (Pinus halepensis) and beneath understory vegetation. Burning appears to have increased the frequency of water repellency occurrence, with 74% of burned samples being classified as water repellent compared to 33% from unburned terrain in the composite fraction (< 2 mm). The persistence of water repellency after fire was highly variable but nevertheless showed statistical differences at p < 0.001 comparing burned vs unburned. Moreover, statistical differences in the persistence of water repellency were found in soil samples taken from beneath pine between burned and unburned, and also beneath understory vegetation. Results showed that soil beneath understory tend to have lower values of water repellency persistence than soil beneath pine. Burned soils showed higher aggregate stability. A positive tendency was observed between aggregate stability and water repellency. The observed changes in WR and AS could have implications for soil hydrological behaviour.
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2007
C Guerrero, R Moral, I Gómez, R Zornoza, V Arcenegui (2007)  Microbial biomass and activity of an agricultural soil amended with the solid phase of pig slurries   Bioresource Technology 17: 3259-3264  
Abstract: Information about the mineralisation rates and effects on soil microorganisms must be obtained prior to the rational use of organic wastes in agriculture or forestry. The objective of this work was to study the mineralisation of two manures derived from the solid phase of pig slurries and the effects on the soil microbial biomass of an agricultural soil. Samples of this soil were mixed at two different rates with two manures derived from the solid phase of pig slurry (composted, CSP, and non-composted, NSP), and then were incubated during 163 days. Carbon mineralised from manures was fitted to first-order kinetic model, and small differences were found between manures despite the composting of one of them. Approximately 45% of the C added was mineralised in the experimental period. The soil microbial biomass C (Cmic) was increased by the amendments according to the application rate. The sudden increases of the qCO2 in the treated samples were ephemeral. The most appreciable differences between these manures were those related with net N mineralisation, being greater in the NSP-treated samples. The application of the solid phase of pig slurries, composted or not, could be a feasible practice to enhance in a short-term the microbial biomass of agricultural soils. In order to avoid an excessive release of inorganic N, the use of composted materials is preferred
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R Zornoza, C Guerrero, J Mataix-Solera, V Arcenegui, F García-Orenes, J Mataix-Beneyto (2007)  Assessing the effects of air-drying and rewetting pre-treatment on soil microbial biomass, basal respiration, metabolic quotient and soluble carbon under Mediterranean conditions   European Journal of Soil Biology 43: 120-129  
Abstract: Soil biochemical properties are useful indicators of soil quality as they are very sensitive to disturbance. Sample storage or pre-treatments could affect the results in these assays, which are normally determined on fresh samples, kept cold or frozen. The objectives of this study were to (i) evaluate the effect of air-drying or incubation of rewetted air-dried soil samples on microbial biomass carbon (MBC), basal soil respiration (BSR), qCO2 and water soluble carbon (WSC), in soils from different locations, with different degradation status and sampling seasons, and (ii) assess if air-drying or incubation of rewetted air-dried soil samples is an accurate sample storage and pre-treatment procedure for these soil properties in soil quality evaluations under semiarid Mediterranean conditions. Our results showed that air-drying does not have the same effects on MBC, BSR, qCO2 and WSC depending on the geographical situation and sampling date. It seems that the warmest and driest place and season show less variation when using air-dried soil samples, with values representative of those obtained under field-moist conditions. Short incubations (4, 8 and 12 days at 23 °C) provoked a general decrease in all properties, probably due to labile organic compounds depletion. Hence, air-dried soils can be used as part of soil quality analysis to estimate these biochemical properties in summer time in the semiarid region of South-East Spain, because they have not suffered severe affections. Moreover, MBC could also be determined using air-dried soil in the driest zones during all year. In contrast, estimations with incubated soil samples are not, in any case, representative of field-moist soil values.
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C Guerrero, J Mataix-Solera, V Arcenegui, J Mataix-Beneyto, I Gómez (2007)  Near-infrared spectroscopy to estimate the maximum temperatures reached on burned soils   Soil Science Society of America Journal 71: 1029-1037  
Abstract: We studied the use of near-infrared (NIR) reflectance spectroscopy as a potential method to estimate a posteriori the maximum temperatures reached (MTR) on burned soils. When soils are heated, the NIR spectra change in accordance with the MTR. Thus, after calibrating, these patterns of NIR could be used as a fingerprint to estimate the MTR in burned soils. Successful validations of the models relating NIR spectra with MTR were obtained in each of the five soils studied (local models), with r2 values ranging from 97.47 to 98.56%. A global model constructed with samples from the five soils studied obtained a similar accuracy, suggesting the presence in soils of some NIR-detectable compounds with similar thermal sensitivity. The influence of the variability caused by the soil type and the duration of heating during model constructions is also evaluated and discussed. The use of NIR presents interesting advantages, such as low cost, low time consumption, minimal pretreatment of samples, no need for chemicals, and accuracy. The results indicate that the MTR could be estimated in burned soils with NIR, offering a new perspective on studies of wildfire effects on soils.
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V Arcenegui, J Mataix-Solera, C Guerrero, R Zornoza, A M Mayoral, J Morales (2007)  Factors controlling the water repellency induced by fire in calcareous Mediterranean forest soils   European Journal of Soil Science 58: 1254-1259  
Abstract: Water repellency (WR) is a property affected by fire and of crucial importance in the hydrological behaviour of soils after burning. In dry Mediterranean areas knowledge of the factors that control the development of water repellency by fire is of particular interest. We examined such factors in two calcareous soils, a Regosol and a Luvisol, representative of forest areas of southeast Spain. Heating temperature (200â500°C), vegetation type (Rosmarinus officinalis, Pinus halepensis and Brachypodium retusum), quantity of vegetation litter (control, low and high) and type of soil were selected as factors for assessing the WR induced by fire. The two soils exhibited markedly different WR responses after heating, the Regosol being much more susceptible than the Luvisol. Characteristics such as organic matter and clay content seem to determine the different WR responses to heating. We found that the type and quantity of vegetation litter also control the persistence of induced WR. In general, the order of increasing WR was Brachypodium < Pinus < Rosmarinus, and larger amounts of litter induced more WR. Maximum values of WR, most of them classified as severe (901â3600 s), were found in the range of 300â350°C, whereas beyond this temperature WR was destroyed. These results show that water repellency induced by combustion could be limited by environmental factors such as vegetation type and availability of litter, and that soil type and its characteristics also play a decisive role.
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J Mataix-Solera, V Arcenegui, C Guerrero, A M Mayoral, J Morales, J González, F García-Orenes, I Gómez (2007)  Water repellency under different plant species in a calcareous forest soil in a semiarid Mediterranean environment   Hydrological Processes 21: 2300-2309  
Abstract: Water repellency (WR) is a property that has implications on the hydrologic balance in affected soils. In semi-arid areas where water supply is limited, even slight WR may play an important role in the infiltration and spatial distribution of precipitation into the soil. Acidic and sandy-textured soils have been demonstrated to be more prone to develop WR, but there are studies reporting water repellent properties in other soil types. In the present study we investigated soil WR under four plant species in a semi-arid area with a calcareous, medium-textured forest soil. For this purpose, 160 soil samples were taken at micro-sites under different species (Pinus halepensis, Quercus coccifera, Juniperus oxycedrus and Rosmarinus officinalis). Soil WR was measured with the water drop penetration time (WDPT) test on air-dried samples. Samples with WDPT > 5 s were classified as water repellent. WR was present in 20% of the samples, and its persistence ranged mainly between 10 and 30 s. Under P. halepensis and Q. coccifera, WR occurred more frequently (40 and 30%, respectively) than under J. oxycedrus and R. officinalis (only 5% in both cases). In order to know the causes of the difference in occurrence of WR under the selected species and to establish relationships, soil organic matter (SOM) content and pH were measured for a selection of 66 samples, including all 32 water repellent and 34 wettable samples selected from those taken under the four species (n = 66). A negative relationship between WR and pH was found for all species. Moreover, in the case of P. halepensis soil samples, a positive correlation between WR and SOM content was found. A discriminant analysis allowed for the distinction between water repellent and wettable conditions in calcareous soils, based on the information provided by pH, SOM content and vegetation type. Soil pH turned out to be the most important parameter for discrimination. The hydrological and ecological implications from these results are discussed with special focus on the areas in the region extensively afforested with P. halepensis.
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R Zornoza, J Mataix-Solera, C Guerrero, V Arcenegui, A M Mayoral, J Morales, J Mataix-Beneyto (2007)  Soil properties under natural forest in the Alicante Province of Spain   Geoderma 142: 334-341  
Abstract: For millennia, land use in the Mediterranean region has led to situations in which soil has been severely degraded showing high risks of erosion and impoverishment. Thus, the establishment of soil quality indices is considered to be of crucial importance in determining the state of degradation and recovery of soils. Soils from stable forest ecosystems have specific physical, chemical and biological properties due to the conditions in which they developed. Hence, modelling the balance established among different key soil properties from stable forest ecosystems could be used as a soil quality index, because disturbance practices lead to changes in that natural balance. Here we report the establishment of two soil quality indices under Mediterranean semiarid conditions for forest soils in SE Spain, based on the use of multiple linear regressions integrating different physical, chemical and biochemical properties. As we observed the strong influence that climatic factors have on the values of the different soil properties and their relationships, mean annual precipitation was also incorporated in the regression models as a categorical explanatory variable. Model 1, that explains 92% of the variance in soil organic carbon (SOC), showed that SOC can be calculated by a linear combination of 6 physical, chemical and biochemical properties (acid phosphatase, water holding capacity (WHC), electrical conductivity (EC), available phosphorus (P), cation exchange capacity (CEC) and aggregate stability (AS)). Model 2 explains 89% of the variance in SOC, which can be calculated by means of 7 chemical and biochemical properties (urease, phosphatase and β-glucosidase activities, pH, EC, P and CEC). Our results confirm that a balance exists between the soil organic carbon of high quality soils and some other properties widely recognised in soil quality assessments, due to their sensitivity and the information they provide about the functionality of soils. As disturbance practices should be accompanied by the loss of this balance, SOC calculated by the models (SOCc) is no longer an accurate estimation of the actual SOC determined in laboratory (SOCa). Thus, it is possible to obtain a soil quality index by the calculation of the model residuals: Soil Quality Index = model residual = SOCc â SOCa. For a non-disturbed soil, the soil quality index should be 0 (SOCc = SOCa). In contrast, for disturbed soils, SOCc should be lower or higher than the actual SOC, with values in the soil quality index < 0 or > 0.
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F García-Orenes, A Roldán, C Guerrero, J Mataix-Solera, J Navarro-Pedreño, I Gómez, J Mataix-Beneyto (2007)  Effect of irrigation on the survival of total coliforms in three semiarid soils after amendment with sewage sludge.   Waste Management 27: 12. 1815-1819 11  
Abstract: Sewage sludges are increasingly used in soil amendment programmes, although not without risk since they contain, among other potential hazards, high concentrations of total coliform bacteria. In this paper we have studied the effect of irrigation on the survival of total coliforms in three semiarid degraded soils with different agricultural practices. Fresh sewage sludge was added at 50 g kg(-1) soil, and incubated in both the presence and absence of irrigation. The absence of irrigation led to a sharp decrease in the number of total coliforms in all soils, with the bacteria disappearing in 40 days. Irrigation produced a substantial initial increase in the number of coliforms in the three soils, although after 80 days there was none growing in any of the soils. The results showed that there were significant differences in the survival of coliform bacteria due to the presence or absence of irrigation.
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2006
E I Martínez, A Escudero, F T Maestre, A de la Cruz, C Guerrero, A Rubio (2006)  Small-scale patterns of abundance of mosses and lichens forming biological soil crusts in two semi-arid gypsum environments   Australian Journal of Botany 54: 339–348  
Abstract: Despite important advances in the understanding of biological soil crusts and their key role in ecosystem processes in arid and semi-arid environments, little is known about those factors driving the small-scale patterns of abundance and distribution of crust-forming lichens and mosses. We used constrained ordination techniques (RDAs) to test the hypothesis that the spatial patterning of lichens and mosses is related to surface and subsurface soil variables in two semi-arid gypsum environments of Spain. Our results show that the abundance of mosses and lichens forming biological soil crusts was related to a limited set of variables (cover of bare soil and litter, soil respiration, potassium content and aggregate stability). Moreover, they provide some insights into the importance of these variables as drivers of biological soil-crust composition and abundance in semi-arid gypsum environments.
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R Zornoza, C Guerrero, J Mataix-Solera, V Arcenegui, F García-Orenes, J Mataix-Beneyto (2006)  Assessing air-drying and rewetting pretreatment effect on some soil enzime activities under semiarid Mediterranean conditions   Soil Biology and Biochemistry 38: 2125-2134  
Abstract: Soil enzyme activities are useful indicators of soil quality as they are very sensitive to disturbance. Sample storage or pre-treatments could affect the results in these assays, which are normally determined in fresh samples, kept cold or frozen. The objectives of this study were to (i) evaluate the effect of air-drying or air-drying and rewetting on β-glucosidase, acid phosphatase and urease activities in soils from different locations, degradation status and sampling seasons, and (ii) assess if air-drying or air-drying and rewetting is an accurate sample storage and pre-treatment procedure for enzyme activities in soil quality evaluations under semiarid Mediterranean conditions. Our results showed that urease, phosphatase and β-glucosidase activities were hardly affected by air-drying of degraded and non-degraded soils from the two locations studied in all seasons. Short incubations (4, 8 and 12 d at 23 °C) of rewetted air-dried soil at 55% of water-holding capacity showed different patterns depending on the enzyme studied. Urease and β-glucosidase activities were relatively stable during incubation, with several significant (P<0.05) shifts up and down in some soils and samplings. However, acid phosphatase showed an increase in activity with incubation, of between 5% and 50% relative to air-dried samples. These increases followed no pattern and were unrelated to soil characteristics or sampling date. Hence, urease, phosphatase and β-glucosidase activities determined in air-dried soil samples seem to be representative of those obtained under field-moist conditions. In contrast, short incubations of rewetted soil samples can produce fluctuations in these enzyme activities, mainly of acid phosphatase, and estimations in these conditions are not so representative of field-moist soil values.
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J Casado-Vela, S Sellés, J Navarro-Pedreño, M A Bustamante, J Mataix, C Guerrero, I Gomez (2006)  Evaluation of composted sewage sludge as nutritional source for horticultural soils   Waste Management 26: 946-952  
Abstract: Water deficit and soil degradation have become some of the major problems for crop production in semi-arid regions, as it is the South East of Spain. As a matter of fact, considerable productivity loss and risk of erosion have to be taken into account in these areas, especially those with an horticultural use (Davis, 1989). Utilization of sewage sludge in agriculture. Agricultural Progress 64, 72â80]. Horticultural soils are highly vulnerable and prone to erosion, as vegetables are generally fast-growing species under intensive exploitation regimes. High-rate chemical inputs contribute to horticultural soil degradation and have a dramatic effect on soil microbial population and nutrient balance whilst, at the same time, have a counter-effect on price competitiveness of the vegetables to be commercialized. In this paper we monitored variations in physical, chemical and biological properties of a cauliflower plot where four increasing quantities of compost were applied. We carried out a three-stage sampling schedule in order to check the effect of compost applications doses. We conclude that a 2 kg compost/m2 application had a positive effect on physical and biological properties of the soil and provides a supply of nutrients to grow cauliflowers on its surface under intensive exploitation regimes without loss in biomass yield.
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2005
F Rodriguez, C Guerrero, R Moral, H Ayguade, J Mataix-Beneyto (2005)  Effects of Composted and Non-composted Solid Phase of Pig Slurry on N, P, and K Contents in Two Mediterranean Soils   Communications in Soil Science and Plant Analysis 36: 635 - 647  
Abstract: Improvement of organic matter content in Mediterranean soils is a main concern in soil management, especially in highly intensive agricultural areas and in degraded burned soils. Solid pig slurry is a good soil amendment, not only for its high organic matter content but also for the nutrient it can supply. The objective of this study was to estimate the influence of raw and composted application of a solid pig manure on the NPK content of two degraded soils. Two calcareous soils (Typic Xerofluvent, with highly intensive agricultural use, and Lithic Xerorthent, affected by forest fire) were incubated (aerobically, nonleached conditions, 87 days of incubation at 25°C; soil moisture content 60% of WHC), with two manures derived from the solid phase of pig slurry (composted [CSP] and noncomposted [NSP]). The manures were mixed with the soils at two rates, adding 7 and 14 g organic C kg-1 soil, respectively, in 300-g pots. The variations in N, P, and K contents in soils were investigated. Both manure amendments increased organic N in the soils. Mineralization of organic N was affected by the type of soil and the composting of manure. Moreover, significant interactions between manure and soil type were observed. Available P was increased by the amendments also, but large differences have been observed between soils. After manure amendment, a higher increase in available P occurred in the agricultural soil with respect to the burned soil. Both manure types caused a similar increase of available P, but efficiencies (increase in availability with respect to total P added) were greater for the composted manure. The increase in available K was related to its content in the manures, being greater for the noncomposted manure. As expected, an increase in application rate led to higher NPK contents. The use of the solid phase of pig slurry could be a successful practice for improvement of soil fertility, which in turn stimulates the rapid recovery of vegetation in burned soils.
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F T Maestre, A Escudero, I Martinez, C Guerrero, A Rubio (2005)  Does spatial pattern matter to ecosystem functioning? Insights from biological soil crusts   Functional Ecology 19: 566 - 573  
Abstract: 1. Theoretical and modelling studies highlight the importance of the spatial patterns of organisms for ecosystem functioning, stability and dynamics. However, there is little empirical evidence as to their importance as drivers of such ecosystem attributes. 2. We evaluated the relationships between different biological soil crust attributes (spatial pattern, cover and diversity) and ecosystem functioning in two semiarid areas differing in the developmental stage of these crusts. 3. We found a significant direct relationship between spatial pattern and both soil bulk density and respiration (surrogates of ecosystem infiltration and nutrient cycling, respectively) at one of our study sites. However, the strength of such a relationship was lower than that found between attributes such as cover and evenness and the same ecosystem functioning surrogates. 4. Our results represent, to our knowledge, some of the first empirical evidence linking the spatial pattern of a community and ecosystem functioning. They also suggest that the importance of spatial pattern as a driver of ecosystem functioning may depend on community attributes such as cover and diversity, and that its importance may be lower than that of these attributes.
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2003
2002
2001
C Guerrero, I Gómez, R Moral, J Mataix-Solera, J Mataix-Beneyto, T Hernández (2001)  Reclamation of a burned forest soil with municipal waste compost: macronutrient dynamic and improved vegetation cover recovery.   Bioresource Technology 76: 3. 221-227 Feb  
Abstract: The reclamation of burned soils in Mediterranean environments is of paramount importance in order to increase the levels of soil protection and minimise erosion and soil loss. The changes produced in the content of total organic carbon (TOC), N (Kjeldahl) and available P, K, Ca and Mg by the addition of different doses of a municipal solid waste compost to a burned soil were evaluated during one year. The effect of organic amendment on the improvement in the vegetation cover after one year was also evaluated. The organic amendment, particularly at a high dose, increased the TOC and N-Kjeldahl content of the soil in a closely related way. The levels of available K in soil were also enhanced by the organic amendment. Although the effects on all three parameters tended to decrease with time, their values in the amended soils were higher than in the control soil, which clearly indicates the improvement in the chemical quality of the soil brought about by the organic amendment. The available P content did not seem to be influenced by organic treatment, while available Mg levels were higher than in the control during the first 4 months following organic amendment. The application of compost to the burned soil improved its fertility and favoured rapid vegetal recovery, thus minimising the risk of soil erosion.
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2000
1997

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