Abstract: To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420 nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443 nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood brain barrier (BBB) and brain in particular.
