http://publicationslist.org/data/Matthew_Breen/atom.xml Matthew Breenhttp://publicationslist.org/Matthew_Breen$basepathfavicon.icoRecent additions to Matthew Breen's PublicationsList.org pagehttp://publicationslist.org/publications.png2011-03-19T20:03:41Z http://publicationslist.org/Matthew_Breen/refid103 2011-03-19T20:01:08Z

BACKGROUND: Prostate cancer in men has a high mortality and morbidity due to metastatic disease. The pathobiology of prostate cancer metastasis is not well understood and cell lines and animal models that recapitulate the complex nature of the disease are needed. Therefore, the goal of the study was to establish and characterize a new prostate cancer line derived from a dog with spontaneous prosta...<br/><br/>Nanda K Thudi, Sherry T Shu, Chelsea K Martin, Lisa G Lanigan, Murali V P Nadella, Adrie Van Bokhoven, Jillian L Werbeck, Jessica K Simmons, Sridhar Murahari, William C Kisseberth, Matthew Breen, Christina Williams, Ching-Shih Chen, Laurie K McCauley, Evan T Keller, Thomas J Rosol (2011) <i>Prostate</i> <i></i> <i></i> : <br/>

http://publicationslist.org/Matthew_Breen/refid104 2011-03-19T20:01:08Z

ABSTRACT:<br/><br/>Steven E Suter, George W Small, Eric L Seiser, Rachael Thomas, Matthew Breen, Kristy L Richards (2011) <i>BMC Cancer</i> <i></i> <i></i> 11: 1 <br/>

http://publicationslist.org/Matthew_Breen/refid102 2011-03-19T20:01:08Z

Identification of the genomic regions most intimately associated with non-Hodgkin lymphoma (NHL) pathogenesis is confounded by the genetic heterogeneity of human populations. We hypothesize that the restricted genetic variation of purebred dogs, combined with the contrasting architecture of the human and canine karyotypes, will increase the penetrance of fundamental NHL-associated chromosomal aber...<br/><br/>Rachael Thomas, Eric L Seiser, Alison Motsinger-Reif, Luke Borst, Victor E Valli, Kathryn Kelley, Steven E Suter, David Argyle, Kristine Burgess, Jerold Bell, Kerstin Lindblad-Toh, Jaime F Modiano, Matthew Breen (2011) <i>Leuk Lymphoma</i> <i></i> <i></i> : <br/>

http://publicationslist.org/Matthew_Breen/refid105 2011-03-19T20:01:08Z

A 9-year-old female spayed mixed breed dog was evaluated at the University of Florida Small Animal Hospital for marked leukocytosis with no associated clinical signs. CBC abnormalities included marked leukocytosis (106,000/μL), marked monocytosis (78,000/μL), and the presence of 13% blast cells (13,832/μL), supporting a diagnosis of leukemia. Cytopenias and dysplastic changes in other cell line...<br/><br/>Janice A Cruz Cardona, Rowan Milner, A Rick Alleman, Christina Williams, William Vernau, Matthew Breen, Mary Tompkins (2011) <i>Vet Clin Pathol</i> <i></i> <i></i> 40: 1 40-47<br/>

http://publicationslist.org/Matthew_Breen/refid101 2011-03-19T20:02:35Z

Zachary J Reitman, Natasha J Olby, Christopher L Mariani, Rachael Thomas, Matthew Breen, Darell D Bigner, Roger E McLendon, Hai Yan (2010) <i>Int J Cancer</i> <i></i> <i></i> 127: 1 245-246<br/>

http://publicationslist.org/Matthew_Breen/refid106 2011-03-19T20:01:08Z

The hair follicle is considered to be a model system for studying organogenesis. In our initial study using mouse cells (Zheng et al., 2005) we found that new hair follicle formation always starts from an epithelial platform: the epidermal cells aggregate, the aggregates encyst, and from the periphery of the cysts, centrifugally, hair buds, pegs, and follicles form. In this report, we extend our i...<br/><br/>Ying Zheng, Arben Nace, Wei Chen, Krystal Watkins, Luke Sergott, Ying Homan, John L Vandeberg, Matthew Breen, Kurt Stenn (2010) <i>Dev Dyn</i> <i></i> <i></i> 239: 10 2619-2626<br/>

http://publicationslist.org/Matthew_Breen/refid2 2010-02-04T17:46:15Z

Canine transmissible venereal tumor (CTVT) is an infectious disease of dogs. Remarkably, the infectious agent is the cancerous cell itself. To investigate its origin and spread, we collected 37 tumor samples from four continents and determined their evolutionary relationships using microsatellite length differences and microarray-based comparative genomic hybridization (aCGH). The different tumors...<br/><br/>Clare A Rebbeck, Rachael Thomas, Matthew Breen, Armand M Leroi, Austin Burt (2009) <i>Evolution</i> <i></i> <i></i> 63: 9 2340-2349<br/>

http://publicationslist.org/Matthew_Breen/refid6 2009-08-02T01:04:19Z

Studies using the currently available malignant canine mast cell lines and bone marrow-derived cultured mast cells (BMCMCs) have provided an in-depth understanding of normal and neoplastic canine mast cell biology. However, many of the currently available malignant canine mast cell lines possess limitations, including loss of cell surface markers and inability to bind canine IgE. We have recently ...<br/><br/>Tzu-Yin Lin, Rachael Thomas, Pei-Chien Tsai, Matthew Breen, Cheryl A London (2009) <i>Vet Immunol Immunopathol</i> <i></i> <i></i> 127: 1-2 114-124<br/>

http://publicationslist.org/Matthew_Breen/refid4 2009-11-30T22:42:30Z

Numerous attributes render the domestic dog a highly pertinent model for cancer-associated gene discovery. We performed microarray-based comparative genomic hybridization analysis of 60 spontaneous canine intracranial tumors to examine the degree to which dog and human patients exhibit aberrations of ancestrally related chromosome regions, consistent with a shared pathogenesis. Canine gliomas and ...<br/><br/>Rachael Thomas, Shannon E Duke, Huixia J Wang, Tessa E Breen, Robert J Higgins, Keith E Linder, Peter Ellis, Cordelia F Langford, Peter J Dickinson, Natasha J Olby, Matthew Breen (2009) <i>J Neurooncol</i> <i></i> <i></i> 94: 3 333-349<br/>

http://publicationslist.org/Matthew_Breen/refid3 2010-02-04T17:46:15Z

Recurrent chromosomal aberrations in solid tumors can reveal the genetic pathways involved in the evolution of a malignancy and in some cases predict biological behavior. However, the role of individual genetic backgrounds in shaping karyotypes of sporadic tumors is unknown. The genetic structure of purebred dog breeds, coupled with their susceptibility to spontaneous cancers, provides a robust mo...<br/><br/>Rachael Thomas, Huixia J Wang, Pei-Chien Tsai, Cordelia F Langford, Susan P Fosmire, Cristan M Jubala, David M Getzy, Gary R Cutter, Jaime F Modiano, Matthew Breen (2009) <i>Chromosome Res</i> <i></i> <i></i> 17: 3 365-377<br/>

http://publicationslist.org/Matthew_Breen/refid98 2011-03-19T20:02:35Z

Canine transmissible venereal tumor (CTVT) is an intriguing cancer that is transmitted naturally as an allograft by transplantation of viable tumor cells from affected to susceptible dogs. At least initially, the tumor is able to evade the host's immune response; thus, CTVT has potential to provide novel insights into tumor immunobiology. The nature of CTVT as a "contagious" cancer, originating fr...<br/><br/>Rachael Thomas, Clare Rebbeck, Armand M Leroi, Austin Burt, Matthew Breen (2009) <i>Chromosome Res</i> <i></i> <i></i> 17: 7 927-934<br/>

http://publicationslist.org/Matthew_Breen/refid99 2010-02-04T17:46:15Z

The release of an annotated human genome sequence assembly and the emergence of genomics technologies have led to significant advances in our understanding of many human diseases including cancers. As DNA sequencing technology has become less costly, the field of comparative genomics has progressed rapidly and attention has turned now to generating whole genome assemblies and dedicated genomics re...<br/><br/>Matthew Breen (2009) <i>Top Companion Anim Med</i> <i></i> <i></i> 24: 3 113-121<br/>

http://publicationslist.org/Matthew_Breen/refid100 2011-03-19T20:02:35Z

Injection-site-associated sarcomas (ISAS), commonly arising at the site of routine vaccine administration, afflict as many as 22,000 domestic cats annually in the USA. These tumors are typically more aggressive and prone to recurrence than spontaneous sarcomas (non-ISAS), generally receiving a poorer long-term prognosis and warranting a more aggressive therapeutic approach. Although certain clinic...<br/><br/>Rachael Thomas, Victor E Valli, Peter Ellis, Jerold Bell, Elinor K Karlsson, John Cullen, Kerstin Lindblad-Toh, Cordelia F Langford, Matthew Breen (2009) <i>Chromosome Res</i> <i></i> <i></i> 17: 8 987-1000<br/>

http://publicationslist.org/Matthew_Breen/refid15 2009-08-02T01:06:56Z

Humans and dogs have coexisted for thousands of years, during which time we have developed a unique bond, centered on companionship. Along the way, we have developed purebred dog breeds in a manner that has resulted unfortunately in many of them being affected by serious genetic disorders, including cancers. With serendipity and irony the unique genetic architecture of the 21st century genome of M...<br/><br/>M Breen (2008) <i>Cytogenet Genome Res</i> <i></i> <i></i> 120: 1-2 50-60<br/>

http://publicationslist.org/Matthew_Breen/refid17 2009-08-02T01:06:56Z

Advancements in somatic cell gene targeting have been slow due to the finite lifespan of somatic cells and the overall inefficiency of homologous recombination. The rate of homologous recombination is determined by mechanisms of DNA repair, and by the balance between homologous recombination (HR) and non-homologous end joining (NHEJ). A plasmid-to-plasmid, extra chromosomal recombination system wa...<br/><br/>Gretchen M Zaunbrecher, Patrick W Dunne, Bashir Mir, Matthew Breen, Jorge A Piedrahita (2008) <i>Anim Biotechnol</i> <i></i> <i></i> 19: 1 6-21<br/>

http://publicationslist.org/Matthew_Breen/refid5 2009-08-02T01:04:19Z

Molecular cytogenetic studies have been instrumental in defining the nature of numerical and structural chromosome changes in human cancers, but their significance remains to be fully understood. The emergence of high quality genome assemblies for several model organisms provides exciting opportunities to develop novel genome-integrated molecular cytogenetic resources that now permit a comparative...<br/><br/>R Thomas, S E Duke, E K Karlsson, A Evans, P Ellis, K Lindblad-Toh, C F Langford, M Breen (2008) <i>Cytogenet Genome Res</i> <i></i> <i></i> 122: 2 110-121<br/>

http://publicationslist.org/Matthew_Breen/refid16 2009-08-02T01:06:56Z

The pathophysiological similarities shared by many forms of human and canine disease, combined with the sophisticated genomic resources now available for the dog, have placed 'man's best friend' in a position of high visibility as a model system for a variety of biomedical concerns, including cancer. The importance of nonrandom cytogenetic abnormalities in human leukemia and lymphoma was recognize...<br/><br/>Matthew Breen, Jaime F Modiano (2008) <i>Chromosome Res</i> <i></i> <i></i> 16: 1 145-154<br/>

http://publicationslist.org/Matthew_Breen/refid7 2009-08-02T01:04:19Z

The domestic dog continues to represent an influential model organism for comparative biomedical research owing to the numerous genetic and pathophysiological similarities shared between human and dog diseases. The combined availability of a high-quality genome assembly and a 1 Mb-resolution genome-assembly integrated bacterial artificial chromosome (BAC) panel now provides the essential resources...<br/><br/>Andrea C Young, Ewen F Kirkness, Matthew Breen (2008) <i>Chromosome Res</i> <i></i> <i></i> 16: 8 1193-1202<br/>

http://publicationslist.org/Matthew_Breen/refid18 2009-08-02T01:06:56Z

The generation of a 7.5x dog genome assembly provides exciting new opportunities to interpret tumor-associated chromosome aberrations at the biological level. We present a genomic microarray for array comparative genomic hybridization (aCGH) analysis in the dog, comprising 275 bacterial artificial chromosome (BAC) clones spaced at intervals of approximately 10 Mb. Each clone has been positioned ac...<br/><br/>Rachael Thomas, Shannon E Duke, Stephanie K Bloom, Tessa E Breen, Andrea C Young, Erika Feiste, Eric L Seiser, Pei-Chien Tsai, Cordelia F Langford, Peter Ellis, Elinor K Karlsson, Kerstin Lindblad-Toh, Matthew Breen (2007) <i>J Hered</i> <i></i> <i></i> 98: 5 474-484<br/>

http://publicationslist.org/Matthew_Breen/refid19 2009-08-02T01:06:56Z

The significance of p16/Rb tumor suppressor pathway inactivation in T-cell non-Hodgkin's lymphoma (NHL) remains incompletely understood. We used naturally occurring canine NHL to test the hypothesis that p16 inactivation has specific pathologic correlates. Forty-eight samples (22 T-cell NHL and 26 B-cell NHL) were included. As applicable, metaphase- or array-based comparative genomic hybridization...<br/><br/>S P Fosmire, R Thomas, C M Jubala, J W Wojcieszyn, V E O Valli, D M Getzy, T L Smith, L A Gardner, M G Ritt, J S Bell, K P Freeman, B E Greenfield, S E Lana, W C Kisseberth, S C Helfand, G R Cutter, M Breen, J F Modiano (2007) <i>Vet Pathol</i> <i></i> <i></i> 44: 4 467-478<br/>

http://publicationslist.org/Matthew_Breen/refid24 2009-08-02T01:07:26Z

J F Modiano, M Breen, V E O Valli, J W Wojcieszyn, G R Cutter (2007) <i>Leukemia</i> <i></i> <i></i> 21: 1 184-187<br/>

http://publicationslist.org/Matthew_Breen/refid23 2009-08-02T01:07:26Z

X-chromosome inactivation (XCI) evolved in mammals to deal with X-chromosome dosage imbalance between the XX female and the XY male. In eutherian mammals, random XCI of the soma requires a master regulatory locus known as the 'X-inactivation center' (XIC/Xic), wherein lies the noncoding XIST/Xist silencer RNA and its regulatory antisense Tsix gene. By contrast, marsupial XCI is imprinted to occur ...<br/><br/>Lance S Davidow, Matthew Breen, Shannon E Duke, Paul B Samollow, John R McCarrey, Jeannie T Lee (2007) <i>Chromosome Res</i> <i></i> <i></i> 15: 2 137-146<br/>

http://publicationslist.org/Matthew_Breen/refid22 2009-08-02T01:07:26Z

The generation of high-quality genome assemblies for numerous species is advancing at a rapid pace. As the number of genome assemblies increases, so does our ability to investigate genome relationships and their contributions to unraveling complex biological, evolutionary, and biomedical processes. A key process in the generation of a genome assembly is to determine and verify the precise physical...<br/><br/>S E Duke, P B Samollow, E Mauceli, K Lindblad-Toh, M Breen (2007) <i>Chromosome Res</i> <i></i> <i></i> 15: 3 361-370<br/>

http://publicationslist.org/Matthew_Breen/refid21 2009-08-02T01:07:26Z

We report a high-quality draft of the genome sequence of the grey, short-tailed opossum (Monodelphis domestica). As the first metatherian ('marsupial') species to be sequenced, the opossum provides a unique perspective on the organization and evolution of mammalian genomes. Distinctive features of the opossum chromosomes provide support for recent theories about genome evolution and function, incl...<br/><br/>Tarjei S Mikkelsen, Matthew J Wakefield, Bronwen Aken, Chris T Amemiya, Jean L Chang, Shannon Duke, Manuel Garber, Andrew J Gentles, Leo Goodstadt, Andreas Heger, Jerzy Jurka, Michael Kamal, Evan Mauceli, Stephen M J Searle, Ted Sharpe, Michelle L Baker, Mark A Batzer, Panayiotis V Benos, Katherine Belov, Michele Clamp, April Cook, James Cuff, Radhika Das, Lance Davidow, Janine E Deakin, Melissa J Fazzari, Jacob L Glass, Manfred Grabherr, John M Greally, Wanjun Gu, Timothy A Hore, Gavin A Huttley, Michael Kleber, Randy L Jirtle, Edda Koina, Jeannie T Lee, Shaun Mahony, Marco A Marra, Robert D Miller, Robert D Nicholls, Mayumi Oda, Anthony T Papenfuss, Zuly E Parra, David D Pollock, David A Ray, Jacqueline E Schein, Terence P Speed, Katherine Thompson, John L VandeBerg, Claire M Wade, Jerilyn A Walker, Paul D Waters, Caleb Webber, Jennifer R Weidman, Xiaohui Xie, Michael C Zody, Jennifer A Marshall Graves, Chris P Ponting, Matthew Breen, Paul B Samollow, Eric S Lander, Kerstin Lindblad-Toh (2007) <i>Nature</i> <i></i> <i></i> 447: 7141 167-177<br/>

http://publicationslist.org/Matthew_Breen/refid20 2009-08-02T01:06:56Z

A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCRalphabeta, TCRgammadelta, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD...<br/><br/>William C Kisseberth, Murali Vara Prasad Nadella, Matthew Breen, Rachael Thomas, Shannon E Duke, Sridhar Murahari, Carrie E Kosarek, William Vernau, Anne C Avery, Mary Jo Burkhard, Thomas J Rosol (2007) <i>Leuk Res</i> <i></i> <i></i> 31: 12 1709-1720<br/>

http://publicationslist.org/Matthew_Breen/refid25 2009-08-02T01:07:26Z

Chand Khanna, Kerstin Lindblad-Toh, David Vail, Cheryl London, Philip Bergman, Lisa Barber, Matthew Breen, Barbara Kitchell, Elizabeth McNeil, Jaime F Modiano, Steven Niemi, Kenine E Comstock, Elaine Ostrander, Susan Westmoreland, Stephen Withrow (2006) <i>Nat Biotechnol</i> <i></i> <i></i> 24: 9 1065-1066<br/>

http://publicationslist.org/Matthew_Breen/refid8 2009-08-02T01:06:18Z

Immunophenotypes in lymphoproliferative diseases (LPD) are prognostically significant, yet causative factors for these conditions, and specifically those associated with heritable risk, remain elusive. The full spectrum of LPD seen in humans occurs in dogs, but the incidence and lifetime risk of naturally occurring LPD differs among dog breeds. Taking advantage of the limited genetic heterogeneity...<br/><br/>Jaime F Modiano, Matthew Breen, Robert C Burnett, Heidi G Parker, Seidu Inusah, Rachael Thomas, Paul R Avery, Kerstin Lindblad-Toh, Elaine A Ostrander, Gary C Cutter, Anne C Avery (2005) <i>Cancer Res</i> <i></i> <i></i> 65: 13 5654-5661<br/>

http://publicationslist.org/Matthew_Breen/refid27 2009-08-02T01:07:53Z

Recognition of the domestic dog as a model for the comparative study of human genetic traits has led to major advances in canine genomics. The pathophysiological similarities shared between many human and dog diseases extend to a range of cancers. Human tumors frequently display recurrent chromosome aberrations, many of which are hallmarks of particular tumor subtypes. Using a range of molecular c...<br/><br/>Rachael Thomas, Allyson Scott, Cordelia F Langford, Susan P Fosmire, Cristan M Jubala, Travis D Lorentzen, Christophe Hitte, Elinor K Karlsson, Ewen Kirkness, Elaine A Ostrander, Francis Galibert, Kerstin Lindblad-Toh, Jaime F Modiano, Matthew Breen (2005) <i>Genome Res</i> <i></i> <i></i> 15: 12 1831-1837<br/>

http://publicationslist.org/Matthew_Breen/refid28 2009-08-02T01:07:53Z

We examined the presence of phosphatase and tensin homolog deleted from chromosome 10 (PTEN) abnormalities that could contribute to the origin or progression of naturally occurring canine endothelial tumors (hemangiosarcoma). Our results document somatic point mutations or deletions encompassing the PTEN C-terminal domain in canine hemangiosarcoma that might provide cells a survival advantage with...<br/><br/>E B Dickerson, R Thomas, S P Fosmire, A R Lamerato-Kozicki, S R Bianco, J W Wojcieszyn, M Breen, S C Helfand, J F Modiano (2005) <i>Vet Pathol</i> <i></i> <i></i> 42: 5 618-632<br/>

http://publicationslist.org/Matthew_Breen/refid29 2009-08-02T01:07:53Z

The pathophysiologic similarities of many human and canine cancers support the role of the domestic dog as a model for brain tumor research. Here we report the construction of a custom canine brain-specific cDNA microarray and the analysis of gene expression patterns of several different types of canine brain tumor. The microarray contained 4000 clones from a canine brain specific cDNA library inc...<br/><br/>S A M Thomson, E Kennerly, N Olby, J R Mickelson, D E Hoffmann, P J Dickinson, G Gibson, M Breen (2005) <i>Vet Pathol</i> <i></i> <i></i> 42: 5 550-558<br/>

http://publicationslist.org/Matthew_Breen/refid9 2009-08-02T01:06:18Z

Pinschers affected by coat color dilution show a specific pigmentation phenotype. The dilute pigmentation phenotype leads to a silver-blue appearance of the eumelanin-containing fur and a pale sandy color of pheomelanin-containing fur. In Pinscher breeding, dilute black-and-tan dogs are called "blue," and dilute red or brown animals are termed "fawn" or "Isabella fawn." Coat color dilution in Pins...<br/><br/>U Philipp, P Quignon, A Scott, C André, M Breen, T Leeb (2005) <i>J Hered</i> <i></i> <i></i> 96: 7 774-776<br/>

http://publicationslist.org/Matthew_Breen/refid26 2009-08-02T01:07:53Z

Here we report a high-quality draft genome sequence of the domestic dog (Canis familiaris), together with a dense map of single nucleotide polymorphisms (SNPs) across breeds. The dog is of particular interest because it provides important evolutionary information and because existing breeds show great phenotypic diversity for morphological, physiological and behavioural traits. We use sequence com...<br/><br/>Kerstin Lindblad-Toh, Claire M Wade, Tarjei S Mikkelsen, Elinor K Karlsson, David B Jaffe, Michael Kamal, Michele Clamp, Jean L Chang, Edward J Kulbokas, Michael C Zody, Evan Mauceli, Xiaohui Xie, Matthew Breen, Robert K Wayne, Elaine A Ostrander, Chris P Ponting, Francis Galibert, Douglas R Smith, Pieter J DeJong, Ewen Kirkness, Pablo Alvarez, Tara Biagi, William Brockman, Jonathan Butler, Chee-Wye Chin, April Cook, James Cuff, Mark J Daly, David DeCaprio, Sante Gnerre, Manfred Grabherr, Manolis Kellis, Michael Kleber, Carolyne Bardeleben, Leo Goodstadt, Andreas Heger, Christophe Hitte, Lisa Kim, Klaus-Peter Koepfli, Heidi G Parker, John P Pollinger, Stephen M J Searle, Nathan B Sutter, Rachael Thomas, Caleb Webber, Jennifer Baldwin, Adal Abebe, Amr Abouelleil, Lynne Aftuck, Mostafa Ait-Zahra, Tyler Aldredge, Nicole Allen, Peter An, Scott Anderson, Claudel Antoine, Harindra Arachchi, Ali Aslam, Laura Ayotte, Pasang Bachantsang, Andrew Barry, Tashi Bayul, Mostafa Benamara, Aaron Berlin, Daniel Bessette, Berta Blitshteyn, Toby Bloom, Jason Blye, Leonid Boguslavskiy, Claude Bonnet, Boris Boukhgalter, Adam Brown, Patrick Cahill, Nadia Calixte, Jody Camarata, Yama Cheshatsang, Jeffrey Chu, Mieke Citroen, Alville Collymore, Patrick Cooke, Tenzin Dawoe, Riza Daza, Karin Decktor, Stuart DeGray, Norbu Dhargay, Kimberly Dooley, Kathleen Dooley, Passang Dorje, Kunsang Dorjee, Lester Dorris, Noah Duffey, Alan Dupes, Osebhajajeme Egbiremolen, Richard Elong, Jill Falk, Abderrahim Farina, Susan Faro, Diallo Ferguson, Patricia Ferreira, Sheila Fisher, Mike FitzGerald, Karen Foley, Chelsea Foley, Alicia Franke, Dennis Friedrich, Diane Gage, Manuel Garber, Gary Gearin, Georgia Giannoukos, Tina Goode, Audra Goyette, Joseph Graham, Edward Grandbois, Kunsang Gyaltsen, Nabil Hafez, Daniel Hagopian, Birhane Hagos, Jennifer Hall, Claire Healy, Ryan Hegarty, Tracey Honan, Andrea Horn, Nathan Houde, Leanne Hughes, Leigh Hunnicutt, M Husby, Benjamin Jester, Charlien Jones, Asha Kamat, Ben Kanga, Cristyn Kells, Dmitry Khazanovich, Alix Chinh Kieu, Peter Kisner, Mayank Kumar, Krista Lance, Thomas Landers, Marcia Lara, William Lee, Jean-Pierre Leger, Niall Lennon, Lisa Leuper, Sarah LeVine, Jinlei Liu, Xiaohong Liu, Yeshi Lokyitsang, Tashi Lokyitsang, Annie Lui, Jan Macdonald, John Major, Richard Marabella, Kebede Maru, Charles Matthews, Susan McDonough, Teena Mehta, James Meldrim, Alexandre Melnikov, Louis Meneus, Atanas Mihalev, Tanya Mihova, Karen Miller, Rachel Mittelman, Valentine Mlenga, Leonidas Mulrain, Glen Munson, Adam Navidi, Jerome Naylor, Tuyen Nguyen, Nga Nguyen, Cindy Nguyen, Thu Nguyen, Robert Nicol, Nyima Norbu, Choe Norbu, Nathaniel Novod, Tenchoe Nyima, Peter Olandt, Barry O'Neill, Keith O'Neill, Sahal Osman, Lucien Oyono, Christopher Patti, Danielle Perrin, Pema Phunkhang, Fritz Pierre, Margaret Priest, Anthony Rachupka, Sujaa Raghuraman, Rayale Rameau, Verneda Ray, Christina Raymond, Filip Rege, Cecil Rise, Julie Rogers, Peter Rogov, Julie Sahalie, Sampath Settipalli, Theodore Sharpe, Terrance Shea, Mechele Sheehan, Ngawang Sherpa, Jianying Shi, Diana Shih, Jessie Sloan, Cherylyn Smith, Todd Sparrow, John Stalker, Nicole Stange-Thomann, Sharon Stavropoulos, Catherine Stone, Sabrina Stone, Sean Sykes, Pierre Tchuinga, Pema Tenzing, Senait Tesfaye, Dawa Thoulutsang, Yama Thoulutsang, Kerri Topham, Ira Topping, Tsamla Tsamla, Helen Vassiliev, Vijay Venkataraman, Andy Vo, Tsering Wangchuk, Tsering Wangdi, Michael Weiand, Jane Wilkinson, Adam Wilson, Shailendra Yadav, Shuli Yang, Xiaoping Yang, Geneva Young, Qing Yu, Joanne Zainoun, Lisa Zembek, Andrew Zimmer, Eric S Lander (2005) <i>Nature</i> <i></i> <i></i> 438: 7069 803-819<br/>

http://publicationslist.org/Matthew_Breen/refid30 2009-08-02T01:08:27Z

Hemangiosarcoma (HSA) is a common untreatable cancer of dogs that resembles human angiosarcoma. Detailed studies of these diseases have been historically hindered by the paucity of suitable reagents. Here, we show that expression of CD117 (c-Kit) can distinguish primitive (malignant) from mature (benign) proliferative endothelial lesions, and we describe eight independent cell lines derived from c...<br/><br/>Susan P Fosmire, Erin B Dickerson, Allyson M Scott, Stacie R Bianco, Marilyn J Pettengill, Heather Meylemans, Marcia Padilla, Ashley A Frazer-Abel, Nasim Akhtar, David M Getzy, John Wojcieszyn, Matthew Breen, Stuart C Helfand, Jaime F Modiano (2004) <i>Lab Invest</i> <i></i> <i></i> 84: 5 562-572<br/>

http://publicationslist.org/Matthew_Breen/refid31 2009-08-02T01:08:27Z

Adult-onset cerebellar cortical degeneration recently has been reported in American Staffordshire Terriers. We describe the clinical and histopathologic features of this disease and examine its mode of inheritance in 63 affected dogs. The age at which neurologic deficits 1st were recognized varied from 18 months to 9 years, with the majority of dogs presented to veterinarians between 4 and 6 years...<br/><br/>Natasha Olby, Stephane Blot, Jean-Laurent Thibaud, Jeff Phillips, Dennis P O'Brien, Jeanne Burr, Jason Berg, Talmage Brown, Matthew Breen (2004) <i>J Vet Intern Med</i> <i></i> <i></i> 18: 2 201-208<br/>

http://publicationslist.org/Matthew_Breen/refid14 2009-08-02T01:06:18Z

We established a canine hemangiosarcoma cell line derived from malignant endothelial cells comprising a spontaneous tumor in a dog to provide a renewable source of endothelial cells for studies of angiogenesis in malignancy. Pieces of the hemangiosarcoma biopsy were engrafted subcutaneously in a bg/nu/XID mouse allowing the tumor cells to expand in vivo. A cell line, SB-HSA, was derived from the x...<br/><br/>Nasim Akhtar, Marcia L Padilla, Erin B Dickerson, Howard Steinberg, Matthew Breen, Robert Auerbach, Stuart C Helfand (2004) <i>Neoplasia</i> <i></i> <i></i> 6: 2 106-116<br/>

http://publicationslist.org/Matthew_Breen/refid10 2009-08-02T01:06:18Z

Comparison of the expression profiles of 2,721 genes in the cerebellum, cortex and pituitary gland of three American Staffordshire terriers, one beagle and one fox hound revealed regional expression differences in the brain but failed to reveal marked differences among breeds, or even individual dogs. Approximately 85 per cent (42 of 49 orthologue comparisons) of the regional differences in the do...<br/><br/>Erin Kennerly, Susanne Thomson, Natasha Olby, Matthew Breen, Greg Gibson (2004) <i>Hum Genomics</i> <i></i> <i></i> 1: 6 435-443<br/>

http://publicationslist.org/Matthew_Breen/refid13 2009-08-02T01:06:18Z

All Dalmatian dogs have an inherited defect in purine metabolism leading to high levels of uric acid excretion in their urine (hyperuricosuria) rather than allantoin, the normal end product of purine metabolism in all other breeds of dog. Transplantation experiments have demonstrated that the defect is intrinsic to the liver and not the kidney. Uricase, the enzyme involved in the breakdown of urat...<br/><br/>D L Bannasch, J R Ryun, M J Bannasch, R H Schaible, M Breen, G Ling (2004) <i>Anim Genet</i> <i></i> <i></i> 35: 4 326-328<br/>

http://publicationslist.org/Matthew_Breen/refid12 2009-08-02T01:06:18Z

BACKGROUND: The 156 breeds of dog recognized by the American Kennel Club offer a unique opportunity to map genes important in genetic variation. Each breed features a defining constellation of morphological and behavioral traits, often generated by deliberate crossing of closely related individuals, leading to a high rate of genetic disease in many breeds. Understanding the genetic basis of both p...<br/><br/>Matthew Breen, Christophe Hitte, Travis D Lorentzen, Rachael Thomas, Edouard Cadieu, Leah Sabacan, Allyson Scott, Gwenaelle Evanno, Heidi G Parker, Ewen F Kirkness, Ruth Hudson, Richard Guyon, Gregory G Mahairas, Boris Gelfenbeyn, Claire M Fraser, Catherine André, Francis Galibert, Elaine A Ostrander (2004) <i>BMC Genomics</i> <i></i> <i></i> 5: 1 <br/>

http://publicationslist.org/Matthew_Breen/refid11 2009-08-02T01:06:18Z

A high-density map of the region of canine Chromosome 5 (CFA5) surrounding the evolutionary breakpoint between human Chromosomes 1p32 and 17pll was constructed by integrating a radiation hybrid map including 41 microsatellites, 10 BACs, and 59 genes and a linkage map including 18 markers. A collection of canine genomic survey sequences providing 1.5x coverage was used to identify dog orthologs of ...<br/><br/>Kenine E Comstock, Frode Lingaas, Ewen F Kirkness, Christophe Hitte, Rachael Thomas, Matthew Breen, Francis Galibert, Elaine A Ostrander (2004) <i>Mamm Genome</i> <i></i> <i></i> 15: 7 544-551<br/>

http://publicationslist.org/Matthew_Breen/refid1 2009-08-02T01:01:15Z

BACKGROUND: The 156 breeds of dog recognized by the American Kennel Club offer a unique opportunity to map genes important in genetic variation. Each breed features a defining constellation of morphological and behavioral traits, often generated by deliberate crossing of closely related individuals, leading to a high rate of genetic disease in many breeds. Understanding the genetic basis of both p...<br/><br/>Matthew Breen, Christophe Hitte, Travis D Lorentzen, Rachael Thomas, Edouard Cadieu, Leah Sabacan, Allyson Scott, Gwenaelle Evanno, Heidi G Parker, Ewen F Kirkness, Ruth Hudson, Richard Guyon, Gregory G Mahairas, Boris Gelfenbeyn, Claire M Fraser, Catherine André, Francis Galibert, Elaine A Ostrander (2004) <i>BMC Genomics</i> <i></i> <i></i> 5: 1 <br/>

http://publicationslist.org/Matthew_Breen/refid38 2009-08-02T01:08:59Z

Hereditary multifocal renal cystadenocarcinoma and nodular dermatofibrosis (RCND) is a naturally occurring canine kidney cancer syndrome that was originally described in German Shepherd dogs. The disease is characterized by bilateral, multifocal tumors in the kidneys, uterine leiomyomas and nodules in the skin consisting of dense collagen fibers. We previously mapped RCND to canine chromosome 5 (C...<br/><br/>Frode Lingaas, Kenine E Comstock, Ewen F Kirkness, Anita Sørensen, Tone Aarskaug, Christophe Hitte, Michael L Nickerson, Lars Moe, Laura S Schmidt, Rachael Thomas, Matthew Breen, Francis Galibert, Berton Zbar, Elaine A Ostrander (2003) <i>Hum Mol Genet</i> <i></i> <i></i> 12: 23 3043-3053<br/>

http://publicationslist.org/Matthew_Breen/refid39 2009-08-02T01:08:59Z

Myotubular/centronuclear myopathies are a nosological group of hereditary disorders characterised by severe architectural and metabolic remodelling of skeletal muscle fibres. In most myofibres, nuclei are found at an abnormal central position within a halo devoid of myofibrillar proteins. The X-linked form (myotubular myopathy) is the most prevalent and severe form in human, leading to death durin...<br/><br/>Laurent Tiret, Stéphane Blot, Jean-Louis Kessler, Hugues Gaillot, Matthew Breen, Jean-Jacques Panthier (2003) <i>Hum Genet</i> <i></i> <i></i> 113: 4 297-306<br/>

http://publicationslist.org/Matthew_Breen/refid40 2009-08-02T01:08:59Z

Chromosome (chr) X is under-represented in current maps of the genome of the domestic dog ( Canis familiaris). To address this problem, we have constructed a small-insert, genomic DNA library in pBluescript from flow-sorted canine Chr X DNA. Fluorescence in situ hybridization (FISH) studies confirmed that the library was highly enriched for Chr X. Clones containing microsatellites were identified ...<br/><br/>Helen F Spriggs, Nigel G Holmes, Matthew G Breen, Panos G Deloukas, Cordelia F Langford, Mark T Ross, Nigel P Carter, Maria E Davis, Catherine E Knights, Angela E Smith, Christine J Farr, Linda C McCarthy, Matthew M Binns (2003) <i>Mamm Genome</i> <i></i> <i></i> 14: 3 214-221<br/>

http://publicationslist.org/Matthew_Breen/refid37 2009-08-02T01:08:27Z

Recurrent chromosome aberrations are frequently observed in human neoplastic cells and often correlate with other clinical and histopathological parameters of a given tumour type. The clinical presentation, histology and biology of many canine cancers closely parallels those of human malignancies. Since humans and dogs demonstrate extensive genome homology and share the same environment, it is exp...<br/><br/>R Thomas, K C Smith, E A Ostrander, F Galibert, M Breen (2003) <i>Br J Cancer</i> <i></i> <i></i> 89: 8 1530-1537<br/>

http://publicationslist.org/Matthew_Breen/refid32 2009-08-02T01:08:27Z

As with many human cancers, canine tumors demonstrate recurrent chromosome aberrations. A detailed knowledge of such aberrations may facilitate diagnosis, prognosis and the selection of appropriate therapy. Following recent advances made in human genomics, we are developing a DNA microarray for the domestic dog, to be used in the detection and characterization of copy number changes in canine tumo...<br/><br/>R Thomas, H Fiegler, E A Ostrander, F Galibert, N P Carter, M Breen (2003) <i>Cytogenet Genome Res</i> <i></i> <i></i> 102: 1-4 254-260<br/>

http://publicationslist.org/Matthew_Breen/refid36 2009-08-02T01:08:27Z

U Philipp, P Quignon, A Scott, S Rak, C André, M Breen, T Leeb (2003) <i>Cytogenet Genome Res</i> <i></i> <i></i> 101: 1 <br/>

http://publicationslist.org/Matthew_Breen/refid34 2009-08-02T01:08:27Z

The analysis of inherited diseases in the domestic dog (Canis familiaris) provides a resource for the continued use of this species as a model system for human diseases. Many different dog breeds are affected by congenital sensorineural deafness. Since mutations in various genes have already been found causative for sensorineural hearing impairment in humans or mice, 20 of these genes were conside...<br/><br/>S G Rak, C Drögemüller, T Leeb, P Quignon, C André, A Scott, M Breen, O Distl (2003) <i>Cytogenet Genome Res</i> <i></i> <i></i> 101: 2 130-135<br/>

http://publicationslist.org/Matthew_Breen/refid33 2009-08-02T01:08:27Z

An extensive number of genes have been implicated in the initiation and progression of human cancers, aiding our understanding of the genetic aetiology of this highly heterogeneous disease. In order to facilitate extrapolation of such information between species, we have isolated and physically mapped the canine orthologues of 25 well-characterised human cancer-related genes. The identity of PCR p...<br/><br/>R Thomas, W Bridge, K Benke, M Breen (2003) <i>Cytogenet Genome Res</i> <i></i> <i></i> 102: 1-4 249-253<br/>

http://publicationslist.org/Matthew_Breen/refid35 2009-08-02T01:08:27Z

U Philipp, A Scott, P Quignon, C André, M Breen, T Leeb (2003) <i>Cytogenet Genome Res</i> <i></i> <i></i> 101: 1 <br/>

http://publicationslist.org/Matthew_Breen/refid41 2009-08-02T01:08:59Z

M Breen (2002) <i>Equine Vet J</i> <i></i> <i></i> 34: 2 110-111<br/>

http://publicationslist.org/Matthew_Breen/refid47 2009-08-02T01:09:31Z

We have isolated six ATOX1 loci from the canine genome in BAC clones. Sequence analysis showed that five of these clones correspond to processed pseudogenes. Fluorescent in situ hybridization allowed us to map the genuine ATOX1 gene to CFA4q24-->q31 and the ATOX1 pseudogenes to CFA19q13.1, CFA4q24-->q31, CFA18q24-->q25, CFA9q22.1 -->q22.2 and CFA20q11-->q12.<br/><br/>B van de Sluis, M S Nanji, M Breen, P L Pearson, B A Oost, D W Cox, C Wijmenga (2001) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 93: 1-2 105-108<br/>

http://publicationslist.org/Matthew_Breen/refid46 2009-08-02T01:09:31Z

Cross-species chromosome painting analyses have recently demonstrated the presence of regions of conserved synteny between the human and domestic dog genomes, aiding the search for candidate genes for inherited traits. Concerted efforts to subchromosomally assign substantial numbers of dog gene sequences are now needed in order to refine these comparative data, both in terms of marker density and ...<br/><br/>R Thomas, M Breen, M M Binns (2001) <i>Anim Genet</i> <i></i> <i></i> 32: 3 148-151<br/>

http://publicationslist.org/Matthew_Breen/refid48 2009-08-02T01:09:31Z

The transglutaminase 1 gene (TGM1) encodes an enzyme necessary for cross-linking the structural proteins that form the cornified envelope, an essential component of the outermost layer of the skin, the stratum corneum. Reported here is the complete coding region of canine TGM1, its chromosome localization, and its map position in the integrated canine linkage-radiation hybrid map. Canine TGM1 cons...<br/><br/>K M Credille, P J Venta, M Breen, J K Lowe, K E Murphy, E A Ostrander, F Galibert, R W Dunstan (2001) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 93: 1-2 73-76<br/>

http://publicationslist.org/Matthew_Breen/refid42 2009-08-02T01:09:31Z

Mutations in the gene for gamma-sarcoglycan (SGCG) located on HSA 13q12 are responsible for limb girdle muscular dystrophy (LGMD2C) in human. Here we report the cloning of the canine SGCG gene together with its genomic structure and several intragenic polymorphisms. The coding part of the canine SGCG contains seven exons spanning at least 70 kb of genomic DNA. The chromosome assignment of the cani...<br/><br/>K Conrad, A Deppe, S Neumann, M Breen, P Quignon, C André, B Brenig, T Leeb (2001) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 94: 3-4 186-189<br/>

http://publicationslist.org/Matthew_Breen/refid43 2009-08-02T01:09:31Z

We present the molecular cytogenetic analysis of a novel case of canine lymphoma, in a nine-year-old entire male collie cross retriever dog that presented with an enlarged prescapular lymph node. A diagnosis of high-grade lymphoblastic lymphoma was made by histological evaluation of fixed lymph node biopsy sections, whilst immunohistochemical analyses demonstrated co-expression of B- and T-cell an...<br/><br/>R Thomas, K C Smith, R Gould, S M Gower, M M Binns, M Breen (2001) <i>Chromosome Res</i> <i></i> <i></i> 9: 8 649-657<br/>

http://publicationslist.org/Matthew_Breen/refid49 2009-08-02T01:09:31Z

We describe the construction of a dog embryonic head/neck cDNA library and the isolation of the dog homolog of the Treacher Collins Syndrome gene, TCOF1. The protein shows a similar three-domain structure to that described for human TCOF1, but the dog gene lacks exon 10 and contains two exons not present in the human sequence. In addition, exon 19 is differentially spliced in the dog. How these st...<br/><br/>K E Haworth, I Islam, M Breen, W Putt, E Makrinou, M Binns, D Hopkinson, Y Edwards (2001) <i>Mamm Genome</i> <i></i> <i></i> 12: 8 622-629<br/>

http://publicationslist.org/Matthew_Breen/refid44 2009-08-02T01:09:31Z

We present here the first fully integrated, comprehensive map of the canine genome, incorporating detailed cytogenetic, radiation hybrid (RH), and meiotic information. We have mapped a collection of 266 chromosome-specific cosmid clones, each containing a microsatellite marker, to all 38 canine autosomes by fluorescence in situ hybridization (FISH). A 1500-marker RH map, comprising 1078 microsatel...<br/><br/>M Breen, S Jouquand, C Renier, C S Mellersh, C Hitte, N G Holmes, A Chéron, N Suter, F Vignaux, A E Bristow, C Priat, E McCann, C André, S Boundy, P Gitsham, R Thomas, W L Bridge, H F Spriggs, E J Ryder, A Curson, J Sampson, E A Ostrander, M M Binns, F Galibert (2001) <i>Genome Res</i> <i></i> <i></i> 11: 10 1784-1795<br/>

http://publicationslist.org/Matthew_Breen/refid54 2009-08-02T01:10:27Z

We report fluorescence in-situ hybridization (FISH) and somatic cell hybrid mapping data for 13 different horse genes (ANP, CD2, CLU, CRISP3, CYP17, FGG, IL1RN, IL10, MMP13, PRM1, PTGS2, TNFA and TP53). Primers for PCR amplification of intronic or untranslated regions were designed from horse-specific DNA or mRNA sequences in GenBank. Two different horse bacterial artificial chromosome (BAC) libra...<br/><br/>G Lindgren, M Breen, S Godard, A Bowling, J Murray, M Scavone, L Skow, K Sandberg, G Guérin, M Binns, H Ellegren (2001) <i>Chromosome Res</i> <i></i> <i></i> 9: 1 53-59<br/>

http://publicationslist.org/Matthew_Breen/refid55 2009-08-02T01:10:27Z

Domestic dog breeds show a wide variety of morphologies and offer excellent opportunities to study the molecular genetics of phenotypic traits. We are interested in exploring this potential and have begun by investigating the genetic basis of a short-tail trait. Our focus has been on the T gene, which encodes a T-box transcription factor important for normal posterior mesoderm development. Haploin...<br/><br/>K Haworth, W Putt, B Cattanach, M Breen, M Binns, F Lingaas, Y H Edwards (2001) <i>Mamm Genome</i> <i></i> <i></i> 12: 3 212-218<br/>

http://publicationslist.org/Matthew_Breen/refid50 2009-08-02T01:09:31Z

S Debenham, P Ricketts, N G Holmes, R Thomas, M Breen, M Binns (2001) <i>Anim Genet</i> <i></i> <i></i> 32: 1 50-51<br/>

http://publicationslist.org/Matthew_Breen/refid53 2009-08-02T01:10:27Z

The development of a detailed genome map for the domestic dog (Canis familiaris, CFA) is a prerequisite for the continued use of this species as a model system for the study of inherited traits. We present an integrated cytogenetic, radiation-hybrid, and comparative map of dog Chromosome (Chr) 5 (CFA 5). The map comprises 14 gene markers, selected from loci previously mapped within the correspondi...<br/><br/>R Thomas, M Breen, P Deloukas, N G Holmes, M M Binns (2001) <i>Mamm Genome</i> <i></i> <i></i> 12: 5 371-375<br/>

http://publicationslist.org/Matthew_Breen/refid52 2009-08-02T01:10:27Z

The MSX2 gene encodes a homeodomain transcription factor important for normal head and face morphogenesis. MSX2 is expressed in key craniofacial structures during development and mutations in the human gene give rise to various craniofacial abnormalities. We are interested in the genetic basis of non-pathogenic variation in skull and face shape. As part of this study we have analysed DNA from a pa...<br/><br/>K Haworth, M Breen, M Binns, D A Hopkinson, Y H Edwards (2001) <i>Anim Genet</i> <i></i> <i></i> 32: 1 32-36<br/>

http://publicationslist.org/Matthew_Breen/refid51 2009-08-02T01:10:27Z

A horse bacterial artificial chromosome (BAC) library was screened for 19 microsatellite markers from unassigned or non-oriented linkage groups. Clones containing 11 (AHT20, EB2E8, HMS45, LEX005, LEX014, LEX023, LEX044, TKY111, UCDEQ425, UCDEQ464 and VIASH21) of these were found, which were from eight different linkage groups. The BAC clones were used as probes in dual colour FISH to identify thei...<br/><br/>G Lindgren, J E Swinburne, M Breen, D Mariat, K Sandberg, G Guérin, H Ellegren, M M Binns (2001) <i>Anim Genet</i> <i></i> <i></i> 32: 1 37-39<br/>

http://publicationslist.org/Matthew_Breen/refid61 2009-08-02T01:10:27Z

Two 3-generation full-sibling reference families have been produced and form a unique resource for genetic linkage mapping studies in the horse. The F(2) generations, now comprising 61 individuals, consist of 28- to 32-day-old embryos removed nonsurgically from two pairs of identical twin mares. The same stallion sired all F(2)s such that the two full-sibling families are half-sibling with respect...<br/><br/>J Swinburne, C Gerstenberg, M Breen, V Aldridge, L Lockhart, E Marti, D Antczak, M Eggleston-Stott, E Bailey, J Mickelson, K Røed, G Lindgren, W von Haeringen, G Guérin, J Bjarnason, T Allen, M Binns (2000) <i>Genomics</i> <i></i> <i></i> 66: 2 123-134<br/>

http://publicationslist.org/Matthew_Breen/refid62 2009-08-02T01:10:27Z

Abnormalities in the genes encoding Pit-1 and Prop-1 have been reported to cause combined pituitary hormone deficiency (CPHD) in mice and humans. In dogs, a similar phenotype has been described in the German shepherd breed. We have previously reported that the Pit-1 gene (POU1F1) is not mutated in affected German shepherd dogs. In this study, we report the isolation and mapping of the canine Prop-...<br/><br/>I S Lantinga-van Leeuwen, H S Kooistra, J A Mol, C Renier, M Breen, B A van Oost (2000) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 88: 1-2 140-144<br/>

http://publicationslist.org/Matthew_Breen/refid65 2009-08-02T01:10:27Z

Combined pituitary hormone deficiency (CPHD) is an autosomal recessive inherited disease of German shepherd dogs characterized primarily by dwarfism. In mice and humans a similar genetic disorder has been described that results from an alteration in the gene encoding the transcription factor Pit-1. In this study we characterized the canine Pit-1 gene, determined the chromosomal localization of the...<br/><br/>I S Lantinga-van Leeuwen, J A Mol, H S Kooistra, A Rijnberk, M Breen, C Renier, B A van Oost (2000) <i>Mamm Genome</i> <i></i> <i></i> 11: 1 31-36<br/>

http://publicationslist.org/Matthew_Breen/refid60 2009-08-02T01:10:27Z

Analysis of genomic sequence from canine chromosome 20q15.1-->q15.2 revealed the presence of two closely linked genes. The two genes represent the corresponding canine orthologs of human aminomethyltransferase (AMT) and the human T-cell leukemia translocation associated (TCTA) gene. Aminomethyltransferase or glycine cleavage system T-protein is an important enzyme in glycine metabolism. The report...<br/><br/>T Leeb, M Breen, B Brenig (2000) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 89: 1-2 98-100<br/>

http://publicationslist.org/Matthew_Breen/refid63 2009-08-02T01:10:27Z

Dystroglycan is a laminin binding protein, which provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix. It is also involved in the organization of basement membranes. So far the genomic organization of the dystroglycan gene DAG1 has not been completely investigated. Here we report the cloning and sequencing of 162 kb of dog genomic DNA containing the compl...<br/><br/>T Leeb, S Neumann, A Deppe, M Breen, B Brenig (2000) <i>Genome Res</i> <i></i> <i></i> 10: 3 295-301<br/>

http://publicationslist.org/Matthew_Breen/refid59 2009-08-02T01:10:27Z

J E Swinburne, L Lockhart, V Aldridge, E Marti, M Breen, M M Binns (2000) <i>Anim Genet</i> <i></i> <i></i> 31: 3 237-238<br/>

http://publicationslist.org/Matthew_Breen/refid56 2009-08-02T01:10:27Z

A Krempler, M Breen, B Brenig (2000) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 90: 1-2 66-67<br/>

http://publicationslist.org/Matthew_Breen/refid57 2009-08-02T01:10:27Z

We have isolated a recombinant phage harboring the canine CALC-I/alpha-CGRP gene. The gene spans a region of approx. 5.3 kb and consists of six exons with sizes ranging from 95 bp (exon 2) and 494 bp (exon 4). By alternative splicing, two transcripts with ORFs of 390 and 384 nt are generated. These encode either the 32-amino acid-long hormone calcitonin (CALC) or the neurotransmitter calcitonin ge...<br/><br/>S Wende, A Krempler, M Breen, L Brunnberg, B Brenig (2000) <i>Mamm Genome</i> <i></i> <i></i> 11: 9 736-740<br/>

http://publicationslist.org/Matthew_Breen/refid58 2009-08-02T01:10:27Z

Recurrent chromosome aberrations are associated with many human cancers. Detailed cytogenetic analysis of tumors has benefited enormously from the development of molecular cytogenetic techniques based on fluorescence in situ hybridization (FISH). Comparative genomic hybridization (CGH) is a recently developed FISH technique that allows a rapid and comprehensive identification of imbalanced genomic...<br/><br/>K A Dunn, R Thomas, M M Binns, M Breen (2000) <i>Vet J</i> <i></i> <i></i> 160: 1 77-82<br/>

http://publicationslist.org/Matthew_Breen/refid71 2009-08-02T01:12:23Z

Our laboratories are interested in characterizing genes involved in the myriad of heritable diseases affecting the domestic dog, Canis lupus familiaris, and in development of detailed genetic and physical maps of the canine genome. Included in these efforts is examination of conservation of the genetic organization, structure, and function of gene families involved in diseases of the canine skin, ...<br/><br/>A B Miller, M Breen, K E Murphy (1999) <i>Mamm Genome</i> <i></i> <i></i> 10: 4 371-375<br/>

http://publicationslist.org/Matthew_Breen/refid72 2009-08-02T01:12:23Z

The majority of microsatellite markers being used to generate the emerging genetic linkage maps of the dog are derived from small-insert, random clones. While such markers are easy to generate, they have the disadvantage that they cannot easily be physically mapped by fluorescence in situ hybridization (FISH), making it difficult to assess the extent of genome coverage represented by such maps. In...<br/><br/>H F Dickens, N G Holmes, E Ryder, M Breen, R Thomas, N Suter, J Sampson, C F Langford, M Ross, N P Carter, M M Binns (1999) <i>J Hered</i> <i></i> <i></i> 90: 1 52-54<br/>

http://publicationslist.org/Matthew_Breen/refid74 2009-08-02T01:12:23Z

Abnormal hepatic copper accumulation is recognized as an inherited disorder in man, mouse, rat and dog. The major cause of hepatic copper accumulation in man is a dysfunctional ATP7B gene, causing Wilson disease (WD). Mutations in the ATP7B genes have also been demonstrated in mouse and rat. The ATP7B gene has been excluded in the much rarer human copper overload disease non-Indian childhood cirrh...<br/><br/>B J van de Sluis, M Breen, M Nanji, M van Wolferen, P de Jong, M M Binns, P L Pearson, J Kuipers, J Rothuizen, D W Cox, C Wijmenga, B A van Oost (1999) <i>Hum Mol Genet</i> <i></i> <i></i> 8: 3 501-507<br/>

http://publicationslist.org/Matthew_Breen/refid70 2009-08-02T01:12:23Z

We have determined the molecular basis for skeletal myopathy and dilated cardiomyopathy in two male German short-haired pointer (GSHP) littermates. Analysis of skeletal muscle demonstrated a complete absence of dystrophin on Western blot analysis. PCR analysis of genomic DNA revealed a deletion encompassing the entire dystrophin gene. Molecular cytogenetic analysis of lymphocytes from the dam and ...<br/><br/>S J Schatzberg, N J Olby, M Breen, L V Anderson, C F Langford, H F Dickens, S D Wilton, C J Zeiss, M M Binns, J N Kornegay, G E Morris, N J Sharp (1999) <i>Neuromuscul Disord</i> <i></i> <i></i> 9: 5 289-295<br/>

http://publicationslist.org/Matthew_Breen/refid75 2009-08-02T01:12:23Z

Protein C is a precursor to a serine protease present in the plasma that plays an important physiological role in the regulation of blood coagulation. Mutations in the human protein C gene have been linked to some cases of Morbus Perthes disease, a thrombophilic condition that results in aseptic necrosis of the femur head and neck. We have cloned the canine protein C gene to investigate whether Mo...<br/><br/>T Leeb, T Kopp, A Deppe, M Breen, U Matis, L Brunnberg, B Brenig (1999) <i>Mamm Genome</i> <i></i> <i></i> 10: 2 134-139<br/>

http://publicationslist.org/Matthew_Breen/refid73 2009-08-02T01:12:23Z

The karyotype of the domestic dog (Canis familiaris) is widely accepted as one of the most difficult mammalian karyotypes to work with. The dog has a total of 78 chromosomes; all 76 autosomes are acrocentric in morphology and show only a gradual decrease in length. Standardization of the canine karyotype has been performed in two stages. The first stage dealt only with chromosomes 1-21 which can b...<br/><br/>M Breen, C F Langford, N P Carter, N G Holmes, H F Dickens, R Thomas, N Suter, E J Ryder, M Pope, M M Binns (1999) <i>J Hered</i> <i></i> <i></i> 90: 1 27-30<br/>

http://publicationslist.org/Matthew_Breen/refid69 2009-08-02T01:12:23Z

We previously reported the identification of a male-specific 658-bp DNA sequence in dogs. We used a specific primer pair designed for PCR amplification of this fragment with DNA samples from 238 dogs, 6 dingoes and 12 wolves. All 133 male samples amplified the 658-bp sequence, whereas all female samples did not. The sequence was not amplified from male DNA samples representing other wild canids (j...<br/><br/>M Olivier, M Breen, M M Binns, G Lust (1999) <i>Chromosome Res</i> <i></i> <i></i> 7: 3 223-233<br/>

http://publicationslist.org/Matthew_Breen/refid64 2009-08-02T01:10:27Z

Conserved segments of synteny between the human genome and chromosome 5 (CFA 5) of the domestic dog (Canis familiaris) have been identified by reciprocal chromosome painting analysis. A CFA 5 paint probe was applied to human metaphase spreads, revealing distinct hybridisation sites on human (HSA) chromosomes 1, 11, 16, and 17. Paint probes for these human chromosomes were then hybridised to dog me...<br/><br/>R Thomas, M Breen, C F Langford, M M Binns (1999) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 87: 1-2 4-10<br/>

http://publicationslist.org/Matthew_Breen/refid66 2009-08-02T01:10:27Z

T Leeb, M Breen, B Brenig (1999) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 86: 3-4 331-332<br/>

http://publicationslist.org/Matthew_Breen/refid68 2009-08-02T01:10:27Z

The domestic dog (Canis familiaris) is widely used as a model in the study of human disease. However, many of the 78 chromosomes comprising the canine karyotype are extremely difficult to identify reliably by classical cytogenetics. This has been a major hindrance to molecular cytogenetic studies of this species. The Animal Health Trust and the Sanger Centre have developed a set of canine whole ch...<br/><br/>M Breen, J Bullerdiek, C F Langford (1999) <i>Chromosome Res</i> <i></i> <i></i> 7: 5 401-406<br/>

http://publicationslist.org/Matthew_Breen/refid67 2009-08-02T01:10:27Z

The domestic dog is increasingly being recognized as a useful model for human disease. The aim of this study was to conduct the first detailed whole-genome comparison of human and dog using bidirectional heterologous chromosome painting (reciprocal Zoo-FISH) analysis. We used whole-chromosome paint probes produced from degenerate oligonucleotide-primed PCR amplification of high-resolution bivariat...<br/><br/>M Breen, R Thomas, M M Binns, N P Carter, C F Langford (1999) <i>Genomics</i> <i></i> <i></i> 61: 2 145-155<br/>

http://publicationslist.org/Matthew_Breen/refid78 2009-08-02T01:12:23Z

T L Lear, M Breen, F A Ponce de Leon, L Coogle, E M Ferguson, T M Chambers, E Bailey (1998) <i>Chromosome Res</i> <i></i> <i></i> 6: 4 333-335<br/>

http://publicationslist.org/Matthew_Breen/refid79 2009-08-02T01:12:23Z

Z Irvin, J Giffard, R Brandon, M Breen, K Bell (1998) <i>Anim Genet</i> <i></i> <i></i> 29: 1 <br/>

http://publicationslist.org/Matthew_Breen/refid76 2009-08-02T01:12:23Z

A primary male autosomal linkage map of the domestic horse (Equus caballus) has been developed by segregation analysis of 140 genetic markers within eight half-sib families. The family material comprised four Standardbred trotters and four Icelandic horses, with a total of 263 offspring. The marker set included 121 microsatellite markers, eight protein polymorphisms, five RFLPs, three blood group ...<br/><br/>G Lindgren, K Sandberg, H Persson, S Marklund, M Breen, B Sandgren, J Carlstén, H Ellegren (1998) <i>Genome Res</i> <i></i> <i></i> 8: 9 951-966<br/>

http://publicationslist.org/Matthew_Breen/refid77 2009-08-02T01:12:23Z

E Marti, M Breen, P Fischer, J Swinburne, M M Binns (1998) <i>Anim Genet</i> <i></i> <i></i> 29: 3 236-238<br/>

http://publicationslist.org/Matthew_Breen/refid45 2009-08-02T01:09:31Z

Binns, Holmes, Breen (1998) <i>ILAR J</i> <i></i> <i></i> 39: 2-3 177-181<br/>

http://publicationslist.org/Matthew_Breen/refid84 2009-08-02T01:12:24Z

Two restriction fragments of bovine papillomavirus type 5 (BPV5), of a genomic size of 1.6 and 1.2 kb were subcloned and sequenced. One of them seemed to correspond to the 3' end of the E1 open reading frame (ORF) and the other to the region of the E7, E8 and 5' end of the E1 ORF. Alignments of these fragments with other BPVs showed that BPV5 is only distantly related to the other 5 BPVs.<br/><br/>N Bloch, M Breen (1997) <i>Virus Genes</i> <i></i> <i></i> 14: 3 171-174<br/>

http://publicationslist.org/Matthew_Breen/refid97 2009-08-02T01:12:24Z

R Thomas, N G Holmes, P E Fischer, H F Dickens, M Breen, J Sampson, M M Binns (1997) <i>Anim Genet</i> <i></i> <i></i> 28: 2 153-154<br/>

http://publicationslist.org/Matthew_Breen/refid85 2009-08-02T01:12:24Z

N Bloch, R H Sutton, M Breen, P B Spradbrow (1997) <i>Vet Res Commun</i> <i></i> <i></i> 21: 1 63-68<br/>

http://publicationslist.org/Matthew_Breen/refid83 2009-08-02T01:12:24Z

Twenty equine microsatellites were isolated from a genomic phage library, and their genetical and physical localization was sought by linkage mapping and fluorescent in situ hybridization (FISH). Nineteen of the markers were found to be polymorphic with, in most cases, heterozygosities exceeding 50%. The markers were mapped in a Swedish reference family for gene mapping, comprising eight half-sib ...<br/><br/>M Breen, G Lindgren, M M Binns, J Norman, Z Irvin, K Bell, K Sandberg, H Ellegren (1997) <i>Mamm Genome</i> <i></i> <i></i> 8: 4 267-273<br/>

http://publicationslist.org/Matthew_Breen/refid82 2009-08-02T01:12:24Z

M Breen, C F Langford, N P Carter, P E Fischer, E Marti, C Gerstenberg, W R Allen, T L Lear, M M Binns (1997) <i>Vet J</i> <i></i> <i></i> 153: 3 235-238<br/>

http://publicationslist.org/Matthew_Breen/refid81 2009-08-02T01:12:24Z

A T Bowling, M Breen, B P Chowdhary, K Hirota, T Lear, L V Millon, F A Ponce de Leon, T Raudsepp, G Stranzinger (1997) <i>Chromosome Res</i> <i></i> <i></i> 5: 7 433-443<br/>

http://publicationslist.org/Matthew_Breen/refid80 2009-08-02T01:12:24Z

J E Swinburne, E Marti, M Breen, M M Binns (1997) <i>Anim Genet</i> <i></i> <i></i> 28: 6 <br/>

http://publicationslist.org/Matthew_Breen/refid86 2009-08-02T01:12:24Z

A lesion on the head of a steer, defined histologically as an epithelial papilloma, yielded DNA which did not hybridise with any of the bovine papillomavirus DNAs usually associated with the formation of skin lesions. DNA from the lesion did hybridise with DNA from bovine papillomavirus 4, even under stringent conditions, and contained a sequence that could be amplified by polymerase chain reactio...<br/><br/>N Bloch, M Breen, Z V Irvin, P B Spradbrow (1996) <i>Vet Rec</i> <i></i> <i></i> 138: 17 414-416<br/>

http://publicationslist.org/Matthew_Breen/refid89 2009-08-02T01:12:24Z

Current evidence suggests that aniridia (absence of iris) is caused by loss of function of one copy of the PAX6 gene, which maps to 11p13. We present the further characterisation of two aniridia pedigrees in which the disease segregates with chromosomal rearrangements which involve 11p13 but do not disrupt the PAX6 gene. We have isolated three human YAC clones which encompass the PAX6 locus and we...<br/><br/>J Fantes, B Redeker, M Breen, S Boyle, J Brown, J Fletcher, S Jones, W Bickmore, Y Fukushima, M Mannens (1995) <i>Hum Mol Genet</i> <i></i> <i></i> 4: 3 415-422<br/>

http://publicationslist.org/Matthew_Breen/refid90 2009-08-02T01:12:24Z

The human growth-arrest specific gene GAS1 maps to chromosome bands 9q21.3-->q22, the region known to contain the tumour suppressor gene responsible for nevoid basal cell carcinoma syndrome (NBCCS). Because of its putative action as a tumour suppressor gene, the GAS1 gene was analysed as a candidate for the NBCCS gene. Using two-colour fluorescence in situ hybridization, the GAS1 gene maps outside...<br/><br/>C Wicking, M Breen, K Negus, J Berkman, A Evdokiou, P Cowled, G Chenevix-Trench, B Wainwright (1995) <i>Cytogenet Cell Genet</i> <i></i> <i></i> 68: 1-2 119-121<br/>

http://publicationslist.org/Matthew_Breen/refid88 2009-08-02T01:12:24Z

N Bloch, R H Sutton, M Breen, P B Spradbrow (1995) <i>Aust Vet J</i> <i></i> <i></i> 72: 7 273-274<br/>

http://publicationslist.org/Matthew_Breen/refid87 2009-08-02T01:12:24Z

Eleven apparent mutations of the equine plasma transferrin and esterase gene (10 in TF and one in ES) were found in an analysis of approximately 240,000 thoroughbred horses. Eight of the transferrin mutations produced variants not previously recognized in horses. In the two remaining transferrin mutations and the esterase mutation, reduced plasma concentrations of the proteins were demonstrated by...<br/><br/>K Bell, H Arthur, M Breen (1995) <i>Anim Genet</i> <i></i> <i></i> 26: 6 407-411<br/>

http://publicationslist.org/Matthew_Breen/refid94 2009-08-02T01:12:24Z

M Breen, P Downs, Z Irvin, K Bell (1994) <i>Anim Genet</i> <i></i> <i></i> 25: 2 <br/>

http://publicationslist.org/Matthew_Breen/refid95 2009-08-02T01:12:24Z

J Gosden, M Breen, D Lawson (1994) <i>Methods Mol Biol</i> <i></i> <i></i> 29: 479-492<br/>

http://publicationslist.org/Matthew_Breen/refid93 2009-08-02T01:12:24Z

M Breen, P Downs, Z Irvin, K Bell (1994) <i>Anim Genet</i> <i></i> <i></i> 25: 2 <br/>

http://publicationslist.org/Matthew_Breen/refid92 2009-08-02T01:12:24Z

Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). Sequences that could be amplified by primers specific for BPV1 and BPV2 were present in 56 of the 76 sarcoids (73%). A restriction site present...<br/><br/>N Bloch, M Breen, P B Spradbrow (1994) <i>Vet Microbiol</i> <i></i> <i></i> 41: 1-2 163-172<br/>

http://publicationslist.org/Matthew_Breen/refid91 2009-08-02T01:12:24Z

Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was c...<br/><br/>M Breen, P Downs, Z Irvin, K Bell (1994) <i>Anim Genet</i> <i></i> <i></i> 25: 6 401-405<br/>

http://publicationslist.org/Matthew_Breen/refid96 2009-08-02T01:12:24Z

Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for effici...<br/><br/>M Breen, B Arveiler, I Murray, J R Gosden, D J Porteous (1992) <i>Genomics</i> <i></i> <i></i> 13: 3 726-730<br/>