http://publicationslist.org/data/a.gradi/atom.xml Alessandra Gradihttp://publicationslist.org/a.gradi$basepathfavicon.icoRecent additions to Alessandra Gradi's PublicationsList.org pagehttp://publicationslist.org/publications.png2010-12-10T17:21:19Z http://publicationslist.org/a.gradi/refid1 2010-12-10T17:20:06Z

B Cecchinelli, A Porrello, C Lazzari, A Gradi, G Bossi, M D'Angelo, A Sacchi, S Soddu (2006) <i>Cell Death Differ</i> <i></i> <i></i> 13: 11 1994-1997<br/>

http://publicationslist.org/a.gradi/refid2 2010-12-10T17:20:06Z

Foot-and-mouth disease virus (FMDV) induces a very rapid inhibition of host cell protein synthesis within infected cells. This is accompanied by the cleavage of the eukaryotic translation initiation factor 4GI (eIF4GI). The cleavage of the related protein eIF4GII has now been analyzed. Within FMDV-infected cells, cleavage of eIF4GI and eIF4GII occurs with similar kinetics. Cleavage of eIF4GII is i...<br/><br/>Alessandra Gradi, Nicole Foeger, Rebecca Strong, Yuri V Svitkin, Nahum Sonenberg, Tim Skern, Graham J Belsham (2004) <i>J Virol</i> <i></i> <i></i> 78: 7 3271-3278<br/>

http://publicationslist.org/a.gradi/refid3 2010-12-10T17:20:06Z

Several picornaviruses shut down host cellular protein synthesis by proteolytic cleavage of the eukaryotic initiation factor (eIF) 4GI and eIF4GII isoforms. Viral RNA translation is maintained by a cap-independent mechanism. Here, we identify the human rhinovirus 2 2A(pro) cleavage site in eIF4GII in vitro as PLLNV(699)*GSR; this sequence lies seven amino acids C-terminal to the cleavage site prev...<br/><br/>Alessandra Gradi, Yuri V Svitkin, Wolfgang Sommergruber, Hiroaki Imataka, Shigenobu Morino, Tim Skern, Nahum Sonenberg (2003) <i>J Virol</i> <i></i> <i></i> 77: 8 5026-5029<br/>

http://publicationslist.org/a.gradi/refid4 2010-12-10T17:20:06Z

Infection of mammalian cells with picornaviruses like entero-, rhino-, and aphthoviruses leads to an inhibition of cap-dependent cellular protein synthesis by the cleavage of both translation initiation factors, eIF4GI and eIF4GII. In entero- and rhinovirus infection this cleavage process is mediated by the viral 2A proteinase (2A(pro)). In order to discriminate between a direct mode of eIF4G clea...<br/><br/>Hans Dieter Liebig, Joachim Seipelt, Elena Vassilieva, Alessandra Gradi, Ernst Kuechler (2002) <i>FEBS Lett</i> <i></i> <i></i> 523: 1-3 53-57<br/>

http://publicationslist.org/a.gradi/refid5 2010-12-10T17:20:06Z

Eukaryotic translation initiation factor 4G (eIF4G), which has two homologs known as eIF4GI and eIF4GII, functions in a complex (eIF4F) which binds to the 5' cap structure of cellular mRNAs and facilitates binding of capped mRNA to 40S ribosomal subunits. Disruption of this complex in enterovirus-infected cells through eIF4G cleavage is known to block this step of translation initiation, thus lead...<br/><br/>W E Marissen, A Gradi, N Sonenberg, R E Lloyd (2000) <i>Cell Death Differ</i> <i></i> <i></i> 7: 12 1234-1243<br/>

http://publicationslist.org/a.gradi/refid6 2010-12-10T17:20:06Z

The eukaryotic translation initiation factor 4G (eIF4G) proteins play a critical role in the recruitment of the translational machinery to mRNA. The eIF4Gs are phosphoproteins. However, the location of the phosphorylation sites, how phosphorylation of these proteins is modulated and the identity of the intracellular signaling pathways regulating eIF4G phosphorylation have not been established. In ...<br/><br/>B Raught, A C Gingras, S P Gygi, H Imataka, S Morino, A Gradi, R Aebersold, N Sonenberg (2000) <i>EMBO J</i> <i></i> <i></i> 19: 3 434-444<br/>

http://publicationslist.org/a.gradi/refid7 2010-12-10T17:20:06Z

A cell line was generated that expresses the poliovirus 2A protease in an inducible manner. Tightly controlled expression was achieved by utilizing the muristerone A-regulated expression system. Upon induction, cleavage of the eukaryotic translation initiation factor 4GI (eIF4GI) and eIF4GII is observed, with the latter being cleaved in a somewhat slower kinetics. eIF4G cleavage was accompanied by...<br/><br/>D Goldstaub, A Gradi, Z Bercovitch, Z Grosmann, Y Nophar, S Luria, N Sonenberg, C Kahana (2000) <i>Mol Cell Biol</i> <i></i> <i></i> 20: 4 1271-1277<br/>

http://publicationslist.org/a.gradi/refid8 2010-12-10T17:20:06Z

For many members of the Picornaviridae family, infection of cells results in a shutoff of host protein synthesis. For rhinoviruses and enteroviruses, the shutoff has been explained in part by the cleavage of eukaryotic initiation factor 4GI (eIF4GI), a component of the cap-binding protein complex eIF4F. The cleavage of eIF4GI is mediated by the virus-specific proteinase 2Apro and results in inhibi...<br/><br/>Y V Svitkin, A Gradi, H Imataka, S Morino, N Sonenberg (1999) <i>J Virol</i> <i></i> <i></i> 73: 4 3467-3472<br/>

http://publicationslist.org/a.gradi/refid11 2010-12-10T17:20:06Z

Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a cap-binding protein complex consisting of three subunits: eIF4E, eIF4A, and eIF4G. In yeast and plants, two related eIF4G species are encoded by two different genes. To date, however, only one functional eIF4G polypeptide, referred to here as eIF4GI, has been identified in mammals. Here we describe the discovery and functional char...<br/><br/>A Gradi, H Imataka, Y V Svitkin, E Rom, B Raught, S Morino, N Sonenberg (1998) <i>Mol Cell Biol</i> <i></i> <i></i> 18: 1 334-342<br/>

http://publicationslist.org/a.gradi/refid10 2010-12-10T17:20:06Z

Eukaryotic initiation factor (eIF) 4GI is a component of the cap-binding protein complex eIF4F, which is required for cap-dependent translation. Infection of cells by poliovirus results in a precipitous decline of host cell protein synthesis, which is preceded by the cleavage of eIF4GI. Cleavage of eIF4GI results in the inhibition of cap-dependent translation. Poliovirus translation is not affecte...<br/><br/>A Gradi, Y V Svitkin, H Imataka, N Sonenberg (1998) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 95: 19 11089-11094<br/>

http://publicationslist.org/a.gradi/refid9 2010-12-10T17:20:06Z

Most eukaryotic mRNAs possess a 5' cap and a 3' poly(A) tail, both of which are required for efficient translation. In yeast and plants, binding of eIF4G to poly(A)-binding protein (PABP) was implicated in poly(A)-dependent translation. In mammals, however, there has been no evidence that eIF4G binds PABP. Using 5' rapid amplification of cDNA, we have extended the known human eIF4GI open reading f...<br/><br/>H Imataka, A Gradi, N Sonenberg (1998) <i>EMBO J</i> <i></i> <i></i> 17: 24 7480-7489<br/>

http://publicationslist.org/a.gradi/refid12 2010-12-10T17:20:06Z

The first enzymatic step in the biosynthesis of steroid hormones occurs in the mitochondrial inner membrane and is dependent on the mobilization of cholesterol from cellular stores. We report on the isolation of a human cDNA which encodes a mitochondrial protein called steroidogenic acute regulatory (StAR) protein, implicated in transport of cholesterol into mitochondria. Nucleotide and predicted ...<br/><br/>A Gradi, R Tang-Wai, H M McBride, L L Chu, G C Shore, J Pelletier (1995) <i>Biochim Biophys Acta</i> <i></i> <i></i> 1258: 3 228-233<br/>

http://publicationslist.org/a.gradi/refid13 2010-12-10T17:20:06Z

The 5'-terminal untranslated region (5' UTR) of the uncapped hepatitis A virus (HAV) RNA contains two pyrimidine-rich sequences; one about 20 nucleotides (nt) in length in the vicinity of the AUG initiation codon (nt 706-726), and a longer one (about 40 nt) encompassing nt 100 to 140. The latter includes a 13 nt 'core' sequence (positions 126-138 in the HM175 strain) which is 80% identical to the ...<br/><br/>J Silveira Carneiro, M Equestre, P Pagnotti, A Gradi, N Sonenberg, R Perez Bercoff (1995) <i>J Gen Virol</i> <i></i> <i></i> 76 ( Pt 5): 1189-1196<br/>