http://publicationslist.org/data/cameronneylon/atom.xml Cameron Neylonhttp://publicationslist.org/cameronneylon$basepathfavicon.icoRecent additions to Cameron Neylon's PublicationsList.org pagehttp://publicationslist.org/publications.png2009-02-15T14:17:38Z http://publicationslist.org/cameronneylon/refid5 2009-02-15T14:15:10Z

Open Science is gathering pace both as a grass roots effort amongst scientists to enable them to share the outputs of their research more effectively, and as a policy initiative for research funders to gain a greater return on their investment. In this workshop, we will discuss the current state of the art in collaborative research tools, the social challenges facing those adopting and advocating ...<br/><br/>Cameron Neylon, Shirley Wu (2009) <i>Pac Symp Biocomput</i> <i></i> <i></i> : 540-544<br/>

http://publicationslist.org/cameronneylon/refid9 2009-02-15T14:15:10Z

Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fas...<br/><br/>Graham R Broder, Rohan T Ranasinghe, Joseph K She, Shahanara Banu, Sam W Birtwell, Gabriel Cavalli, Gerasim S Galitonov, David Holmes, Hugo F P Martins, Kevin F Macdonald, Cameron Neylon, Nikolay Zheludev, Peter L Roach, Hywel Morgan (2008) <i>Anal Chem</i> <i></i> <i></i> 80: 6 1902-1909<br/>

http://publicationslist.org/cameronneylon/refid7 2009-02-15T14:15:10Z

Quasi-elastic neutron scattering (QENS) has been used to study the deviation from Debye-law harmonic behavior in lyophilized and hydrated apoferritin, a naturally occurring, multisubunit protein. Whereas analysis of the measured mean squared displacement (msd) parameter reveals a hydration-dependent inflection above 240 K, characteristic of diffusive motion, a hydration-independent inflection is o...<br/><br/>Mark T F Telling, Cameron Neylon, Susan H Kilcoyne, Valeria Arrighi (2008) <i>J Phys Chem B</i> <i></i> <i></i> 112: 35 10873-10878<br/>

http://publicationslist.org/cameronneylon/refid6 2009-02-15T14:15:10Z

Neutron radiation offers significant advantages for the study of biological molecular structure and dynamics. A broad and significant effort towards instrumental and methodological development to facilitate biology experiments at neutron sources worldwide is reviewed.<br/><br/> Teixeira, Ankner, Bellissent-Funel, Bewley, Blakeley, Coates, Dahint, Dalgliesh, Dencher, Dhont, Fischer, Forsyth, Fragneto, Frick, Geue, Gilles, Gutberlet, Haertlein, Hauß, Häußler, Heller, Herwig, Holderer, Juranyi, Kampmann, Knott, Kohlbrecher, Kreuger, Langan, Lechner, Lynn, Majkrzak, May, Meilleur, Mo, Mortensen, Myles, Natali, Neylon, Niimura, Ollivier, Ostermann, Peters, Pieper, Rühm, Schwahn, Shibata, Soper, Straessle, Suzuki, Tanaka, Tehei, Timmins, Torikai, Unruh, Urban, Vavrin, Weiss, Zaccai (2008) <i>Chem Phys</i> <i></i> <i></i> 345: 2-3 133-151<br/>

http://publicationslist.org/cameronneylon/refid10 2009-02-15T14:15:10Z

Small angle scattering can provide unique structural information on the shape, domain organisation, and interactions of biomacromolecules in solution. Small angle neutron scattering (SANS) combined with deuterium labelling makes it possible to define the positions of specific components within a complex while small angle X-ray scattering (SAXS) provides more precise data on the overall shape. Here...<br/><br/>Cameron Neylon (2008) <i>Eur Biophys J</i> <i></i> <i></i> 37: 5 531-541<br/>

http://publicationslist.org/cameronneylon/refid8 2009-02-15T14:15:10Z

BACKGROUND: Sequencing by hybridisation is an effective method for obtaining large amounts of DNA sequence information at low cost. The efficiency of SBH depends on the design of the probe library to provide the maximum information for minimum cost. Long probes provide a higher probability of non-repeated sequences but lead to an increase in the number of probes required whereas short probes may n...<br/><br/>Niall J Haslam, Nava E Whiteford, Gerald Weber, Adam Prügel-Bennett, Jonathan W Essex, Cameron Neylon (2008) <i>PLoS ONE</i> <i></i> <i></i> 3: 6 <br/>

http://publicationslist.org/cameronneylon/refid1 2009-02-15T14:14:24Z

SU-8 is an epoxy-novolac resin and a well-established negative photoresist for microfabrication and microengineering. The photopolymerized resist is an extremely highly crosslinked polymer showing outstanding chemical and physical robustness with residual surface epoxy groups amenable for chemical functionalization. In this paper we describe, for the first time, the preparation and surface modific...<br/><br/>Gabriel Cavalli, Shahanara Banu, Rohan T Ranasinghe, Graham R Broder, Hugo F P Martins, Cameron Neylon, Hywel Morgan, Mark Bradley, Peter L Roach (2007) <i>J Comb Chem</i> <i></i> <i></i> 9: 3 462-472<br/>

http://publicationslist.org/cameronneylon/refid11 2009-02-15T14:15:10Z

BACKGROUND: There is growing interest in the attachment of proteins to solid supports for the development of supported catalysts, affinity matrices, and micro devices as well as for the development of planar and bead based protein arrays for multiplexed assays of protein concentration, interactions, and activity. A critical requirement for these applications is the generation of a stable linkage b...<br/><br/>Lilyan Chan, Hannah F Cross, Joseph K She, Gabriel Cavalli, Hugo F P Martins, Cameron Neylon (2007) <i>PLoS ONE</i> <i></i> <i></i> 2: 11 <br/>

http://publicationslist.org/cameronneylon/refid2 2009-02-15T14:14:24Z

During chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound Ter sites on approach from one direction but not the other. To study the basis of this polarity, we measured the rates of dissociation of Tus from forked TerB oligonucleotides, such as would be produced by the replicative DnaB helicase at both the fork-blocking (nonpermissive) and permissive ends of the Ter...<br/><br/>Mark D Mulcair, Patrick M Schaeffer, Aaron J Oakley, Hannah F Cross, Cameron Neylon, Thomas M Hill, Nicholas E Dixon (2006) <i>Cell</i> <i></i> <i></i> 125: 7 1309-1319<br/>

http://publicationslist.org/cameronneylon/refid3 2009-02-15T14:14:24Z

Several methods for ultra high-throughput DNA sequencing are currently under investigation. Many of these methods yield very short blocks of sequence information (reads). Here we report on an analysis showing the level of genome sequencing possible as a function of read length. It is shown that re-sequencing and de novo sequencing of the majority of a bacterial genome is possible with read lengths...<br/><br/>Nava Whiteford, Niall Haslam, Gerald Weber, Adam Prügel-Bennett, Jonathan W Essex, Peter L Roach, Mark Bradley, Cameron Neylon (2005) <i>Nucleic Acids Res</i> <i></i> <i></i> 33: 19 <br/>

http://publicationslist.org/cameronneylon/refid4 2009-02-15T14:14:24Z

The arrest of DNA replication in Escherichia coli is triggered by the encounter of a replisome with a Tus protein-Ter DNA complex. A replication fork can pass through a Tus-Ter complex when traveling in one direction but not the other, and the chromosomal Ter sites are oriented so replication forks can enter, but not exit, the terminus region. The Tus-Ter complex acts by blocking the action of the...<br/><br/>Cameron Neylon, Andrew V Kralicek, Thomas M Hill, Nicholas E Dixon (2005) <i>Microbiol Mol Biol Rev</i> <i></i> <i></i> 69: 3 501-526<br/>

http://publicationslist.org/cameronneylon/refid13 2009-02-15T14:17:12Z

Directed molecular evolution and combinatorial methodologies are playing an increasingly important role in the field of protein engineering. The general approach of generating a library of partially randomized genes, expressing the gene library to generate the proteins the library encodes and then screening the proteins for improved or modified characteristics has successfully been applied in the ...<br/><br/>Cameron Neylon (2004) <i>Nucleic Acids Res</i> <i></i> <i></i> 32: 4 1448-1459<br/>

http://publicationslist.org/cameronneylon/refid12 2009-02-15T14:15:10Z

Intein-mediated ligation provides a site-specific method for the attachment of molecular probes to proteins. The method is inherently flexible with regard to either the protein sequence or the attached probe, but practical difficulties have limited the widespread use of this valuable labeling system for the attachment of small- to medium-sized molecules. We report herein studies to improve the eff...<br/><br/>Robert J Wood, David D Pascoe, Zoë K Brown, Emma M Medlicott, Marco Kriek, Cameron Neylon, Peter L Roach (2004) <i>Bioconjug Chem</i> <i></i> <i></i> 15: 2 366-372<br/>

http://publicationslist.org/cameronneylon/refid15 2009-02-15T14:17:12Z

The Escherichia coli replication terminator protein (Tus) binds tightly and specifically to termination sites such as TerB in order to halt DNA replication. To better understand the process of Tus-TerB interaction, an assay based on surface plasmon resonance was developed to allow the determination of the equilibrium dissociation constant of the complex (K(D)) and association and dissocation rate ...<br/><br/>C Neylon, S E Brown, A V Kralicek, C S Miles, C A Love, N E Dixon (2000) <i>Biochemistry</i> <i></i> <i></i> 39: 39 11989-11999<br/>

http://publicationslist.org/cameronneylon/refid14 2009-02-15T14:17:12Z

Despite the fact that homogeneous preparations of isolated cells are now being used very effectively to study a range of important biochemical questions, it is still not known what combination of fuels and energy-producing pathways is used by cells when offered the complex mixture characteristic of plasma or extracellular fluid. We have developed an in vitro system whereby highly purified and func...<br/><br/>M Guppy, L Abas, C Neylon, M E Whisson, S Whitham, D W Pethick, X Niu (1997) <i>Eur J Biochem</i> <i></i> <i></i> 244: 1 161-167<br/>