http://publicationslist.org/data/cesar-sanchez/atom.xml César Sánchezhttp://publicationslist.org/cesar-sanchez$basepathfavicon.icoRecent additions to César Sánchez's PublicationsList.org pagehttp://publicationslist.org/publications.png2008-05-06T16:13:34Z http://publicationslist.org/cesar-sanchez/refid28 2008-05-06T16:09:07Z

C Sánchez, C. Méndez, JA Salas. (2008) <i>Modern Alkaloids: Structure, Isolation, Synthesis and Biology.</i> 619-639<br/>

http://publicationslist.org/cesar-sanchez/refid3 2007-09-05T11:08:41Z

The indolocarbazole family of natural products is a source of lead compounds with potential therapeutic applications in the treatment of cancer and neurodegenerative disorders. Rebeccamycin and staurosporine are two members of this family, which are produced by different actinomycete strains. Although both compounds display antitumor activity, their distinct structural features determine different...<br/><br/>César Sánchez, Carmen Méndez, José A Salas (2006) <i>J Ind Microbiol Biotechnol</i> <i></i> <i></i> 33: 7 560-568<br/>

http://publicationslist.org/cesar-sanchez/refid1 2007-09-05T11:08:41Z

The indolocarbazole family of natural products, including the biosynthetically related bisindolylmaleimides, is reviewed (with 316 references cited). The isolation of indolocarbazoles from natural sources and the biosynthesis of this class of compounds are thoroughly reviewed, including recent developments in molecular genetics, enzymology and metabolic engineering. The biological activities and u...<br/><br/>César Sánchez, Carmen Méndez, José A Salas (2006) <i>Nat Prod Rep</i> <i></i> <i></i> 23: 6 1007-1045<br/>

http://publicationslist.org/cesar-sanchez/refid2 2007-09-05T11:08:41Z

The biosynthetic pathways for violacein and for indolocarbazoles (rebeccamycin, staurosporine) include a decarboxylative fusion of two tryptophan units. However, in the case of violacein, one of the tryptophans experiences an unusual 1-->2 shift of the indole ring. The violacein biosynthetic gene cluster was previously reported to consist of four genes, vioABCD. Here we studied the violacein pathw...<br/><br/>César Sánchez, Alfredo F Braña, Carmen Méndez, José A Salas (2006) <i>Chembiochem</i> <i></i> <i></i> 7: 8 1231-1240<br/>

http://publicationslist.org/cesar-sanchez/refid5 2008-03-03T17:56:23Z

B Shen, M Chen, Y Cheng, L Du, D J Edwards, N P George, Y Huang, T Oh, C Sánchez, G Tang, E Wendt-Pienkowski, F Yi (2005) <i>Biocombinatorial Approaches for Drug Finding (Ernst Schering Res Found Workshop, vol. 51)</i> 107-126<br/>

http://publicationslist.org/cesar-sanchez/refid6 2007-09-05T11:08:41Z

Rebeccamycin and staurosporine are natural products with antitumor properties, which belong to the family of indolocarbazole alkaloids. An intense effort currently exists for the generation of indolocarbazole derivatives for the treatment of several diseases, including cancer and neurodegenerative disorders. Here, we report a biological process based on combinatorial biosynthesis for the productio...<br/><br/>César Sánchez, Lili Zhu, Alfredo F Braña, Aaroa P Salas, Jürgen Rohr, Carmen Méndez, José A Salas (2005) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 102: 2 461-466<br/>

http://publicationslist.org/cesar-sanchez/refid4 2007-09-05T11:08:41Z

The indolocarbazole staurosporine is a potent inhibitor of a variety of protein kinases. It contains a sugar moiety attached through C-N linkages to both indole nitrogen atoms of the indolocarbazole core. Staurosporine biosynthesis was reconstituted in vivo in a heterologous host Streptomyces albus by using two different plasmids: the 'aglycone vector' expressing a set of genes involved in indoloc...<br/><br/>Aaroa P Salas, Lili Zhu, César Sánchez, Alfredo F Braña, Jürgen Rohr, Carmen Méndez, José A Salas (2005) <i>Mol Microbiol</i> <i></i> <i></i> 58: 1 17-27<br/>

http://publicationslist.org/cesar-sanchez/refid22 2008-03-04T21:26:15Z

César Sánchez (2005) <i>Un breve viaje por la ciencia (I Certamen Teresa Pinillos de Ensayos de Divulgación Científica)</i> 31-35<br/>

http://publicationslist.org/cesar-sanchez/refid24 2008-03-03T18:17:50Z

The present invention relates to the biosynthesis of polyketides and derives from the cloning of nucleic acids encoding a polyketide synthase and other associated proteins involved in the synthesis of the polyketide borrelidin. Materials and methods including enzyme systems, nucleic acids, vectors and cells are provided for the preparation of polyketides including borrelidin and analogues and deri...<br/><br/>Olano C, Wilkinson B, Sánchez C, Moss SJ, Braña AF, Martin CJ, Oliynyk M, Méndez C, Leadlay PF, Salas JA (2004) <i>WIPO Publication No. WO2004058976</i><br/>

http://publicationslist.org/cesar-sanchez/refid7 2007-09-05T11:08:41Z

The biosynthetic gene cluster for the angiogenesis inhibitor borrelidin has been cloned from Streptomyces parvulus Tü4055. Sequence analysis indicates that the macrolide ring of borrelidin is formed by a modular polyketide synthase (PKS) (borA1-A6), a result that was confirmed by disruption of borA3. The borrelidin PKS is striking because only seven rather than the nine modules expected for a non...<br/><br/>Carlos Olano, Barrie Wilkinson, César Sánchez, Steven J Moss, Rose Sheridan, Vidya Math, Alison J Weston, Alfredo F Braña, Christine J Martin, Markiyan Oliynyk, Carmen Méndez, Peter F Leadlay, José A Salas (2004) <i>Chem Biol</i> <i></i> <i></i> 11: 1 87-97<br/>

http://publicationslist.org/cesar-sanchez/refid26 2008-03-03T18:28:55Z

The invention relates to a method of obtaining indolocarbazoles using biosynthetic rebeccamycin genes. According to the invention, biosynthetic genes of rebeccamycin from Saccharothrix aerocolonigenes are used for the production of indolocarbazoles in related microorganisms (Streptomyces spp.). The inventive method consists in isolating a DNA fragment of Saccharothrix aerocolonigenes ATCC39243 whi...<br/><br/>Sánchez C, Braña AF, Méndez C, Salas JA (2003) <i>WIPO Publication No. WO03033706</i><br/>

http://publicationslist.org/cesar-sanchez/refid20 2008-05-06T16:05:47Z

Méndez C, Lombó F, Olano C, Sánchez C, Salas JA (2003) <i>Microorganisms for health care, food and enzyme production</i> 23-49<br/>

http://publicationslist.org/cesar-sanchez/refid9 2008-03-03T17:52:44Z

Bleomycin (BLM) biosynthesis has been studied as a model for hybrid peptide-polyketide natural product biosynthesis. Cloning, sequencing, and biochemical characterization of the blm biosynthetic gene cluster from Streptomyces verticillus ATCC15003 revealed that (1) the BLM hybrid peptide-polyketide aglycon is assembled by the BLM megasynthetase that consists of both nonribosomal peptide synthetase...<br/><br/>Ben Shen, Liangcheng Du, César Sánchez, Daniel J Edwards, Mei Chen, Jeffrey M Murrell (2002) <i>J Nat Prod</i> 65: 3 422-431<br/>

http://publicationslist.org/cesar-sanchez/refid8 2008-03-03T16:18:52Z

Rebeccamycin, a halogenated natural product of the indolocarbazole family, is produced by Saccharothrix aerocolonigenes ATCC39243. Several rebeccamycin analogues, which target DNA topoisomerase I or II, have already entered clinical trials as anticancer drugs. Using as a probe an internal fragment of ngt, a Saccharothrix aerocolonigenes gene encoding an indolocarbazole N-glycosyltransferase, we is...<br/><br/>César Sánchez, Igor A Butovich, Alfredo F Braña, Jürgen Rohr, Carmen Méndez, José A Salas (2002) <i>Chem Biol</i> 9: 4 519-531<br/>

http://publicationslist.org/cesar-sanchez/refid10 2008-03-03T17:55:29Z

The hybrid peptide-polyketide backbone of bleomycin (BLM) is assembled by the BLM megasynthetase that consists of both nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) modules. BlmIX/BlmVIII/BlmVII constitute a natural hybrid NRPS/PKS/NRPS system, serving as a model for both hybrid NRPS/PKS and PKS/NRPS systems. Sequence analysis and functional comparison of domains and modules...<br/><br/>B Shen, L Du, C Sánchez, D J Edwards, M Chen, J M Murrell (2001) <i>J Ind Microbiol Biotechnol</i> 27: 6 378-385<br/>

http://publicationslist.org/cesar-sanchez/refid11 2008-03-03T17:55:03Z

BACKGROUND: Phosphopantetheinyl transferases (PPTases) catalyze the posttranslational modification of carrier proteins by the covalent attachment of the 4'-phosphopantetheine (P-pant) moiety of coenzyme A to a conserved serine residue, a reaction absolutely required for the biosynthesis of natural products including fatty acids, polyketides, and nonribosomal peptides. PPTases have been classified ...<br/><br/>César Sánchez, Liangcheng Du, Daniel J Edwards, Michael D Toney, Ben Shen (2001) <i>Chem Biol</i> 8: 7 725-738<br/>

http://publicationslist.org/cesar-sanchez/refid12 2008-03-03T16:23:13Z

A single promoter, rplJp (P(L10)), has been identified in the rplJL operon from Streptomyces coelicolor A3(2) by promoter probe and primer extension analyses. P(L10) is located upstream of the rplL gene and of the DNA encoding the mRNA leader region that contains the putative L10 (or L10.L12(4)) binding site for translational autogenous regulation. The potential start point for transcription was f...<br/><br/>G Blanco, C Sánchez, M R Rodicio, C Méndez, J A Salas (2001) <i>Biochim Biophys Acta</i> 1517: 2 243-249<br/>

http://publicationslist.org/cesar-sanchez/refid13 2008-03-03T17:53:11Z

The structural and catalytic similarities between modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) inspired us to search for hybrid NRPS-PKS systems. By examining the biochemical and genetic data known to date for the biosynthesis of hybrid peptide-polyketide natural products, we show (1) that the same catalytic sites are conserved between the hybrid NRPS-PKS and normal...<br/><br/>Liangcheng Du, César Sánchez, Ben Shen (2001) <i>Metab Eng</i> 3: 1 78-95<br/>

http://publicationslist.org/cesar-sanchez/refid21 2008-05-06T16:06:17Z

Salas JA, Blanco G, Braña AF, Fernández E, Fernández MJ, García-Bernardo J, González A, Lombó F, Prado L, Quirós LM, Sánchez C, Méndez C (2001) <i>Novel frontiers in the production of compounds for biomedical use (Focus on Biotechnology, vol. 1)</i> 383-399<br/>

http://publicationslist.org/cesar-sanchez/refid15 2008-03-03T16:27:40Z

We have previously proposed that the BlmIV and BlmIII non-ribosomal peptide synthetases are involved in the formation of the bithiazole moiety of the anti-tumor drug bleomycin in Streptomyces verticillus ATCC15003. We report here the identification and characterization of an oxidation domain in BlmIII. The oxidation domain shows local homology to a family of oxidoreductases and is present in all t...<br/><br/>L Du, M Chen, C Sánchez, B Shen (2000) <i>FEMS Microbiol Lett</i> 189: 2 171-175<br/>

http://publicationslist.org/cesar-sanchez/refid14 2008-03-03T16:26:23Z

BACKGROUND: The structural and catalytic similarities between modular nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) inspired us to search for a hybrid NRPS-PKS system. The antitumor drug bleomycin (BLM) is a natural hybrid peptide-polyketide metabolite, the biosynthesis of which provides an excellent opportunity to investigate intermodular communication between NRPS and ...<br/><br/>L Du, C Sánchez, M Chen, D J Edwards, B Shen (2000) <i>Chem Biol</i> 7: 8 623-642<br/>

http://publicationslist.org/cesar-sanchez/refid25 2008-03-03T18:23:40Z

This invention provides detailed sequence analysis and characterization of the gene cluster responsible for the synthesis of bleomycin in Streptomyces verticillus. The bleomycin gene cluster provides the first hybrid polyketide synthase/nonribosomal peptide synthetase pathway and elucidation of the various modules and enzymatic domains characterizing the pathway provides convenient synthetic route...<br/><br/>Shen B, Du L, Sánchez C, Chen M, Edwards DJ (2000) <i>WIPO Publication No. WO0040704</i><br/>

http://publicationslist.org/cesar-sanchez/refid16 2008-03-03T16:35:02Z

A DNA chromosomal region of Streptomyces argillaceus ATCC 12596, the producer organism of the antitumor polyketide drug mithramycin, was cloned. Sequence analysis of this DNA region, located between four mithramycin glycosyltransferase genes, showed the presence of two genes (mtmMI and mtmMII) whose deduced products resembled S-adenosylmethionine-dependent methyltransferases. By independent insert...<br/><br/>M J Lozano, L L Remsing, L M Quirós, A F Braña, E Fernández, C Sánchez, C Méndez, J Rohr, J A Salas (2000) <i>J Biol Chem</i> 275: 5 3065-3074<br/>

http://publicationslist.org/cesar-sanchez/refid19 2008-03-03T17:37:30Z

The biosynthesis of bleomycins (BLMs) inStreptomyces verticillus(Sv) ATCC15003 was reviewed. Early biosynthetic studies by incorporation of isotope-labeled precursors and by isolation of biosynthetic intermediates and shunt metabolites were presented to support the hypothesis that (a) BLM is a hybrid peptide and polyketide metabolite and (b) theblmsynthetase, which catalyzes the assembly of BLM fr...<br/><br/>Ben Shen, Liangcheng Du, César Sánchez, Mei Chen, Daniel J. Edwards (1999) <i>Bioorg Chem</i> 27: 2 155<br/>

http://publicationslist.org/cesar-sanchez/refid17 2008-03-03T17:57:21Z

Mithramycin is an antitumor polyketide drug produced by Streptomyces argillaceus that contains two deoxysugar chains, a disaccharide consisting of two D-olivoses and a trisaccharide consisting of a D-olivose, a D-oliose, and a D-mycarose. From a cosmid clone (cosAR3) which confers resistance to mithramycin in streptomycetes, a 3-kb PstI-XhoI fragment was sequenced, and two divergent genes (mtmGI a...<br/><br/>E Fernández, U Weissbach, C Sánchez, A F Braña, C Méndez, J Rohr, J A Salas (1998) <i>J Bacteriol</i> 180: 18 4929-4937<br/>

http://publicationslist.org/cesar-sanchez/refid18 2008-05-06T16:12:03Z

The N-terminal amino acid sequences of two peptides derived from a Streptomyces coelicolor ribosomal protein were determined and degenerate oligonucleotide primers derived from these sequences were used as probes for the screening of a chromosomal DNA library of S. coelicolor. Two positive clones were isolated and DNA sequencing of a 1740-bp region of these clones that hybridised with the probes r...<br/><br/>César Sánchez, Gloria Blanco, Carmen Méndez, J A Salas (1997) <i>Mol Gen Genet</i> 257: 1 91-96<br/>

http://publicationslist.org/cesar-sanchez/refid23 2008-03-03T18:10:20Z

César Sánchez (1997) <br/>

http://publicationslist.org/cesar-sanchez/refid27 2008-03-04T13:34:44Z

This is a microbiology blog, with a focus on actinomycetes (a group of bacteria) and the biosynthesis of natural products with a pharmaceutical interest (antibiotics, antitumor drugs).<br/><br/>César Sánchez () <i>(started on May 2007)</i><br/>