http://publicationslist.org/data/chilakamarti.ramana/atom.xml chilakamarti ramanahttp://publicationslist.org/chilakamarti.ramana$basepathfavicon.icoRecent additions to chilakamarti ramana's PublicationsList.org pagehttp://publicationslist.org/publications.png2008-08-19T15:34:00Z http://publicationslist.org/chilakamarti.ramana/refid4 2008-08-19T15:28:25Z

Human breast cancer (HBC) cell growth suppression by okadaic acid (OA) was previously found to involve elevated expression of oncogenes c-myc and c-fos and apoptosis. Since, c-Myc influences diverse pathways of cell growth, we hypothesized that elevated levels of c-Myc are involved in HBC growth suppression. Here, we investigated whether induction of c-Myc by OA or protein synthesis inhibitor cycl...<br/><br/>Liyue Zhang, Anil Wali, Chilakamarti V Ramana, Arun K Rishi (2007) <i>Cancer Res</i> <i></i> <i></i> 67: 21 10198-10206<br/>

http://publicationslist.org/chilakamarti.ramana/refid3 2008-08-19T15:28:25Z

Macrophages play an important role in immune responses and in inflammatory disease states such as atherosclerosis. Interferon-gamma (IFN-gamma) is a major cytokine involved in the activation of macrophages. To elucidate the primary response of various genes and biological pathways regulated by IFN-gamma in macrophage, we analyzed the gene expression profile in RAW 264.7 macrophage cells treated wi...<br/><br/>Rama S Kota, John C Rutledge, Kishorchandra Gohil, Aseem Kumar, Richard I Enelow, Chilakamarti V Ramana (2006) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 342: 4 1137-1146<br/>

http://publicationslist.org/chilakamarti.ramana/refid2 2008-08-19T15:28:25Z

CD8+ T cell recognition of viral antigens presented by lung epithelial cells is important in the clearance of respiratory viral infection but may cause considerable injury to the lung. We have shown that a critical event of this type of injury is the activation of target epithelial cells and expression of chemokines by these cells. In this study, epithelial gene expression and transcription factor...<br/><br/>Chilakamarti V Ramana, Jyothi Chintapalli, Lumei Xu, Christopher Alia, Jing Zhou, Dunja Bruder, Richard I Enelow (2006) <i>J Interferon Cytokine Res</i> <i></i> <i></i> 26: 5 318-327<br/>

http://publicationslist.org/chilakamarti.ramana/refid8 2008-08-19T15:29:15Z

Lipoprotein lipase (LPL) is a key enzyme in the hydrolysis of triglyceride-rich lipoproteins. In vascular diseases, such as atherosclerosis, inflammation plays an important role in the pathogenesis of the disease. We examined the role of LPL in modulating tumor necrosis factor-alpha (TNF-alpha)- and interferon-gamma (IFN-gamma)-mediated inflammatory cytokine signal transduction pathways in human a...<br/><br/>Rama S Kota, Chilakamarti V Ramana, Fatima A Tenorio, Richard I Enelow, John C Rutledge (2005) <i>J Biol Chem</i> <i></i> <i></i> 280: 35 31076-31084<br/>

http://publicationslist.org/chilakamarti.ramana/refid9 2008-08-19T15:29:15Z

Using microarray technology, we previously demonstrated that IFN-gamma induces suppressor of cytokine signaling-3 (SOCS-3) in Stat1-/- mouse embryonic fibroblasts and bone marrow-derived macrophages. In this study, we have investigated the mechanism by which SOCS-3 is induced by Stat1-independent signal transduction pathway. Tyrosine kinases Jak1 and Jak2 are required for SOCS-3 induction by IFN-g...<br/><br/>Chilakamarti V Ramana, Aseem Kumar, Richard Enelow (2005) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 327: 3 727-733<br/>

http://publicationslist.org/chilakamarti.ramana/refid7 2008-08-19T15:29:15Z

Respiratory virus infection evokes a potent T-cell response that may result in a considerable insult to the structural and functional integrity of the gas exchange units of the lung. Alveolar antigen recognition by CD8+ T lymphocytes results in significant injury that is critically dependent upon tumor necrosis factor (TNF)-alpha expressed by the CD8+ T cells and is largely dependent upon TNF-rece...<br/><br/>Jun Liu, Min Q Zhao, Lumei Xu, C V Ramana, Wim Declercq, Peter Vandenabeele, Richard I Enelow (2005) <i>Am J Respir Cell Mol Biol</i> <i></i> <i></i> 33: 5 463-469<br/>

http://publicationslist.org/chilakamarti.ramana/refid6 2008-08-19T15:29:15Z

p53 has been well characterized as a tumor suppressor gene, but its role in antiviral defense remains unclear. A recent report has demonstrated that p53 can be induced by interferons and is activated after vesicular stomatitis virus (VSV) infection. We observed that different nononcogenic viruses, including encephalomyocarditis virus (EMCV) and human parainfluenza virus type 3 (HPIV3), induced dow...<br/><br/>Joao T Marques, Dominique Rebouillat, Chilakamarti V Ramana, Junko Murakami, Jason E Hill, Andrei Gudkov, Robert H Silverman, George R Stark, Bryan R G Williams (2005) <i>J Virol</i> <i></i> <i></i> 79: 17 11105-11114<br/>

http://publicationslist.org/chilakamarti.ramana/refid5 2008-08-19T15:29:15Z

Proinflammatory cytokines have been linked to depression of myocardial contractility in vivo in patients with acute septic shock and in vitro models employing isolated myocytes exposed to serum from such patients. The key pathways involved in mediating this septic organ dysfunction (cell adhesion molecule expression, inducible nitric-oxide synthase induction, and apoptosis) are known to be regulat...<br/><br/>Aseem Kumar, Anand Kumar, Paul Michael, Danielle Brabant, Amadeo M Parissenti, Chilakamarti V Ramana, Xiulong Xu, Joseph E Parrillo (2005) <i>J Biol Chem</i> <i></i> <i></i> 280: 52 42619-42626<br/>

http://publicationslist.org/chilakamarti.ramana/refid10 2008-08-19T15:29:15Z

Respiratory virus infection results in considerable pulmonary immunopathology, a component of which results from the host immune responses. We have developed a murine model to specifically examine the lung injury due to CD8(+) T cell recognition of an influenza hemagglutinin (HA) transgene on lung epithelium in the absence of replicating virus, after adoptive transfer. Lung injury is largely media...<br/><br/>Lumei Xu, Heesik Yoon, Min Q Zhao, Jun Liu, Chilakamarti V Ramana, Richard I Enelow (2004) <i>J Immunol</i> <i></i> <i></i> 173: 2 721-725<br/>

http://publicationslist.org/chilakamarti.ramana/refid11 2008-08-19T15:30:13Z

Interleukin-1 (IL-1) induces the phosphorylation of Stat1 on serine 727 but not on tyrosine 701. Analyses of mutant I1A cells, which lack the IL-1 receptor-associated kinase (IRAK), and of I1A cells reconstituted with deletion mutants of IRAK show that the IL-1-mediated phosphorylation of Stat1 on serine requires the IRAK protein but not its kinase activity and does not involve phosphatidylinosito...<br/><br/>Hannah Nguyen, Moitreyee Chatterjee-Kishore, Zhengfan Jiang, Yulan Qing, Chilakamarti V Ramana, Joshua Bayes, Mairead Commane, Xiaoxia Li, George R Stark (2003) <i>J Interferon Cytokine Res</i> <i></i> <i></i> 23: 4 183-192<br/>

http://publicationslist.org/chilakamarti.ramana/refid12 2008-08-19T15:30:13Z

The paradigm that emerged from studies during the past decade established a central role for Jak-Stat (signal transducer and activator of transcription) signaling pathways in promoting the diverse cellular responses induced by interferon gamma (IFN-gamma). However, recent studies have shown that the IFN-gamma receptor activates additional signaling pathways and can regulate gene expression by Stat...<br/><br/>Chilakamarti V Ramana, M Pilar Gil, Robert D Schreiber, George R Stark (2002) <i>Trends Immunol</i> <i></i> <i></i> 23: 2 96-101<br/>

http://publicationslist.org/chilakamarti.ramana/refid13 2008-08-19T15:30:13Z

The cytokine gamma interferon (IFN-gamma) and the calcitropic steroid hormone 1,25-dihydroxyvitamin D (1,25D) are activators of macrophage immune function. In sarcoidosis, tuberculosis, and several granulomatoses, IFN-gamma induces 1,25D synthesis by macrophages and inhibits 1,25D induction of 24-hydroxylase, a key enzyme in 1,25D inactivation, causing high levels of 1,25D in serum and hypercalcem...<br/><br/>Marcos Vidal, Chilakamarti V Ramana, Adriana S Dusso (2002) <i>Mol Cell Biol</i> <i></i> <i></i> 22: 8 2777-2787<br/>

http://publicationslist.org/chilakamarti.ramana/refid14 2008-08-19T15:30:13Z

The 90-kDa heat shock protein (Hsp90), the target of the ansamycin class of anti-cancer drugs, is required for the conformational activation of a specific group of signal transducers, including Raf-1. In this report we have identified a 75-kDa Raf-associated protein as Hsp90N, a novel member of the Hsp90 family. Intriguingly, the ansamycin-binding domain is replaced in Hsp90N by a much shorter, hy...<br/><br/>Nicholas Grammatikakis, Adina Vultur, Chilakamarti V Ramana, Aliki Siganou, Clifford W Schweinfest, Dennis K Watson, Leda Raptis (2002) <i>J Biol Chem</i> <i></i> <i></i> 277: 10 8312-8320<br/>

http://publicationslist.org/chilakamarti.ramana/refid18 2008-08-19T15:31:11Z

Although Stat1 is essential for cells to respond fully to IFN-gamma, there is substantial evidence that, in the absence of Stat1, IFN-gamma can still regulate the expression of some genes, induce an antiviral state and affect cell growth. We have now identified many genes that are regulated by IFN-gamma in serum-starved Stat1-null mouse fibroblasts. The proteins induced by IFN-gamma in Stat1-null ...<br/><br/>C V Ramana, M P Gil, Y Han, R M Ransohoff, R D Schreiber, G R Stark (2001) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 98: 12 6674-6679<br/>

http://publicationslist.org/chilakamarti.ramana/refid17 2008-08-19T15:31:11Z

Although Stat1 is required for many IFN-dependent responses, recent work has shown that IFNgamma functions independently of Stat1 to affect the growth of tumor cells or immortalized fibroblasts. We now demonstrate that both IFNgamma and IFNalpha/beta regulate proliferative responses in cells of the mononuclear phagocyte lineage derived from Stat1-null mice. Using both representational difference a...<br/><br/>M P Gil, E Bohn, A K O'Guin, C V Ramana, B Levine, G R Stark, H W Virgin, R D Schreiber (2001) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 98: 12 6680-6685<br/>

http://publicationslist.org/chilakamarti.ramana/refid16 2008-08-19T15:31:11Z

Activation of MAP kinase leads to the activation of p53-dependent pathways, and vice-versa. Although the amount of p53 protein increases in response to MAP kinase-dependent signaling, the basis of this increase is not yet fully understood. We have isolated the mutant cell line AP14, defective in p53 expression, from human HT1080 fibrosarcoma cells, which have an activated ras allele. The expressio...<br/><br/>M L Agarwal, C V Ramana, M Hamilton, W R Taylor, S E DePrimo, L J Bean, A Agarwal, M K Agarwal, A Wolfman, G R Stark (2001) <i>Oncogene</i> <i></i> <i></i> 20: 20 2527-2536<br/>

http://publicationslist.org/chilakamarti.ramana/refid15 2008-08-19T15:30:13Z

STAT1 must be phosphorylated on serine 727 to be fully active in transcription. We show that phosphatidylinositol 3-kinase (PI3K) and its effector kinase Akt play an important role in the serine phosphorylation of STAT1 and in the activation of gene expression in response to interferon-gamma (IFN gamma). IFN gamma activates PI3K as well as Akt in a variety of cell lines. Specific inhibition of PI3...<br/><br/>H Nguyen, C V Ramana, J Bayes, G R Stark (2001) <i>J Biol Chem</i> <i></i> <i></i> 276: 36 33361-33368<br/>

http://publicationslist.org/chilakamarti.ramana/refid19 2008-08-19T15:31:11Z

Stat1 is a fascinating and complex protein with multiple, yet contrasting transcriptional functions. Upon activation, it drives the expression of many genes but also suppresses the transcription of others. These opposing characteristics also apply to its role in facilitating crosstalk between signal transduction pathways, as it participates in both synergistic activation and inhibition of gene exp...<br/><br/>C V Ramana, M Chatterjee-Kishore, H Nguyen, G R Stark (2000) <i>Oncogene</i> <i></i> <i></i> 19: 21 2619-2627<br/>

http://publicationslist.org/chilakamarti.ramana/refid20 2008-08-19T15:31:11Z

Interferons (IFNs) inhibit cell growth in a Stat1-dependent fashion that involves regulation of c-myc expression. IFN-gamma suppresses c-myc in wild-type mouse embryo fibroblasts, but not in Stat1-null cells, where IFNs induce c-myc mRNA rapidly and transiently, thus revealing a novel signaling pathway. Both tyrosine and serine phosphorylation of Stat1 are required for suppression. Induced express...<br/><br/>C V Ramana, N Grammatikakis, M Chernov, H Nguyen, K C Goh, B R Williams, G R Stark (2000) <i>EMBO J</i> <i></i> <i></i> 19: 2 263-272<br/>

http://publicationslist.org/chilakamarti.ramana/refid21 2008-08-19T15:31:11Z

O6-methylguanine-DNA methyltransferase (MGMT), a ubiquitous DNA repair protein, removes the mutagenic DNA adduct O6-alkylguanine, which is synthesized both endogenously and after exposure to alkylnitrosamines and alkylating antitumor drugs such as 2-chloroethyl-N-nitrosourea (CNU). The MGMT gene is highly regulated in mammalian cells and its overexpression, observed in many types of tumor cells, i...<br/><br/>T Biswas, C V Ramana, G Srinivasan, I Boldogh, T K Hazra, Z Chen, K Tano, E B Thompson, S Mitra (1999) <i>Oncogene</i> <i></i> <i></i> 18: 2 525-532<br/>

http://publicationslist.org/chilakamarti.ramana/refid24 2008-08-19T15:32:48Z

Treatment of mouse or human cells with the protein kinase C (PKC) inhibitors H7 or bisindolylmaleimide I induced an increase in the lifetime of p53, leading to its accumulation. In inhibitor-treated cells, p53 translocated to the nuclei and bound to DNA but was not competent to induce transcription. However, transactivation could be induced by subsequent DNA damage. Phorbol ester, a potent activat...<br/><br/>M V Chernov, C V Ramana, V V Adler, G R Stark (1998) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 95: 5 2284-2289<br/>

http://publicationslist.org/chilakamarti.ramana/refid23 2008-08-19T15:31:11Z

Apurinic/apyrimidinic (AP) endonuclease (APE; EC 4.2.99.18) plays a central role in repair of DNA damage due to reactive oxygen species (ROS) because its DNA 3'-phosphoesterase activity removes 3' blocking groups in DNA that are generated by DNA glycosylase/AP-lyases during removal of oxidized bases and by direct ROS reaction with DNA. The major human APE (APE-1) gene is activated selectively by s...<br/><br/>C V Ramana, I Boldogh, T Izumi, S Mitra (1998) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 95: 9 5061-5066<br/>

http://publicationslist.org/chilakamarti.ramana/refid22 2008-08-19T15:31:11Z

O6-Alkylguanine is the major mutagenic and cytotoxic DNA lesion induced by alkylating agents, including 2-chloroethyl-N-nitrosourea-based antitumor drugs. This lesion is repaired by O6-methylguanine-DNA methyltransferase (MGMT), the expression of which is highly variable in both normal tissues and in tumor cells. The promoter of the human MGMT gene was found to contain two putative activator prote...<br/><br/>I Boldogh, C V Ramana, Z Chen, T Biswas, T K Hazra, S Grösch, T Grombacher, S Mitra, B Kaina (1998) <i>Cancer Res</i> <i></i> <i></i> 58: 17 3950-3956<br/>

http://publicationslist.org/chilakamarti.ramana/refid26 2008-08-19T15:32:48Z

N-Methylpurine-DNA glycosylase (MPG), a ubiquitous DNA repair enzyme, removes N-alkylpurines and other purine lesions induced in DNA by simple alkylating carcinogens. A mouse MPG cDNA clone was isolated from a lambda recombinant phage library of BALB/c mouse lung cell and characterized. Using the mouse MPG cDNA as a probe, the complete mouse MPG gene was isolated in two overlapping lambda recombin...<br/><br/>M Tatsuka, G C Ibeanu, T Izumi, S Narayan, C V Ramana, N K Kim, W Kang, G Roy, S Mitra (1995) <i>DNA Cell Biol</i> <i></i> <i></i> 14: 1 37-45<br/>

http://publicationslist.org/chilakamarti.ramana/refid25 2008-08-19T15:32:48Z

Dorsal root ganglia neuronal cell bodies synthesize the vasodilator neuropeptide calcitonin gene-related peptide and innervate the blood vessels and spinal cord sites (laminae I and II) involved in blood pressure regulation. We previously demonstrated that calcitonin gene-related peptide mRNA content is significantly decreased in dorsal root ganglia and that immunoreactive calcitonin gene-related ...<br/><br/>S C Supowit, A Gururaj, C V Ramana, K N Westlund, D J DiPette (1995) <i>Hypertension</i> <i></i> <i></i> 25: 6 1333-1338<br/>

http://publicationslist.org/chilakamarti.ramana/refid27 2008-08-19T15:32:48Z

Calcitonin gene-related peptide, a product of the calcitonin gene, is a potent vasodilator neuropeptide. We have demonstrated that dietary calcium deficiency decreased the neuronal (laminae I/II of the dorsal horn of the spinal cord) content of immunoreactive calcitonin gene-related peptide in the normal rat. Neuronal calcitonin gene-related peptide levels are also reduced in the spontaneously hyp...<br/><br/>S C Supowit, C V Ramana, K N Westlund, D J DiPette (1993) <i>Hypertension</i> <i></i> <i></i> 21: 6 Pt 2 1010-1014<br/>

http://publicationslist.org/chilakamarti.ramana/refid1 2008-08-19T15:27:38Z

Calcitonin gene-related peptide (CGRP), a product of the calcitonin/CGRP gene, is a potent vasodilating neuropeptide widely distributed throughout the cardiovascular system, particularly in the heart. Immunocytochemical studies have demonstrated CGRP-containing neurofibrils in the myocardium and in the periadventitia of coronary blood vessels. Based on these studies, it has been assumed that all o...<br/><br/>C V Ramana, D J DiPette, S C Supowit (1992) <i>Am J Med Sci</i> <i></i> <i></i> 304: 6 339-344<br/>