http://publicationslist.org/data/david.aragao/atom.xml David Aragaohttp://publicationslist.org/david.aragao$basepathfavicon.icoRecent additions to David Aragao's PublicationsList.org pagehttp://publicationslist.org/publications.png2011-06-30T00:16:19Z http://publicationslist.org/david.aragao/refid1 2011-06-30T00:06:54Z

G-protein-coupled receptors (GPCRs) are eukaryotic integral membrane proteins that modulate biological function by initiating cellular signalling in response to chemically diverse agonists. Despite recent progress in the structural biology of GPCRs, the molecular basis for agonist binding and allosteric modulation of these proteins is poorly understood. Structural knowledge of agonist-bound states...<br/><br/>Daniel M Rosenbaum, Cheng Zhang, Joseph A Lyons, Ralph Holl, David Aragao, Daniel H Arlow, Søren G F Rasmussen, Hee-Jung Choi, Brian T Devree, Roger K Sunahara, Pil Seok Chae, Samuel H Gellman, Ron O Dror, David E Shaw, William I Weis, Martin Caffrey, Peter Gmeiner, Brian K Kobilka (2011) <i>Nature</i> <i></i> <i></i> 469: 7329 236-240<br/>

http://publicationslist.org/david.aragao/refid2 2011-06-30T00:06:54Z

Structure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally implemented, would not apply to small protein targets having </=4 transmembrane crossings. In our study, the hypothesis that the inherent flexibility of the mesophase would enable crys...<br/><br/>Nicole Höfer, David Aragão, Martin Caffrey (2010) <i>Biophys J</i> <i></i> <i></i> 99: 3 L23-L25<br/>

http://publicationslist.org/david.aragao/refid3 2011-06-30T00:06:54Z

The hybrid cluster protein (HCP) from the sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough has been isolated and crystallized anaerobically. The phase problem was solved for a P2(1)2(1)2(1) crystal form using multiple-wavelength anomalous diffraction data collected in the vicinity of the Fe K absorption edge. Although the overall protein structure is essentially the same as t...<br/><br/>David Aragão, Edward P Mitchell, Carlos F Frazão, Maria Arménia Carrondo, Peter F Lindley (2008) <i>Acta Crystallogr D Biol Crystallogr</i> <i></i> <i></i> 64: Pt 6 665-674<br/>

http://publicationslist.org/david.aragao/refid4 2011-06-30T00:06:54Z

Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 103 residues, physiologically relevant native form composed by a N-terminal extens...<br/><br/>Carlos Frazão, David Aragão, Ricardo Coelho, Sónia S Leal, Cláudio M Gomes, Miguel Teixeira, Maria Arménia Carrondo (2008) <i>FEBS Lett</i> <i></i> <i></i> 582: 5 763-767<br/>

http://publicationslist.org/david.aragao/refid5 2011-06-30T00:06:54Z

Gellan gum is a widely used commercial material, available in many different forms. Its economic importance has led to studies into the biosynthesis of exopolysaccharide gellan gum, which is industrially prepared in high yields using Sphingomonas elodea ATCC 31461. Glucose-1-phosphate uridylyltransferase mediates the reversible conversion of glucose-1-phosphate and UTP into UDP-glucose and pyropho...<br/><br/>David Aragão, Arsénio M Fialho, Ana R Marques, Edward P Mitchell, Isabel Sá-Correia, Carlos Frazão (2007) <i>J Bacteriol</i> <i></i> <i></i> 189: 12 4520-4528<br/>

http://publicationslist.org/david.aragao/refid6 2011-06-30T00:06:54Z

The cloning, expression, purification, crystallization and preliminary crystallographic analysis of glucose-1-phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose-1-phosphate are reported. Diffraction data sets were obtained from seven crystal forms in five different space groups, with highest resolutions ranging from 4.20 to 2.65 A. The phase problem was solve...<br/><br/>D Aragão, A R Marques, C Frazão, F J Enguita, M A Carrondo, A M Fialho, I Sá-Correia, E P Mitchell (2006) <i>Acta Crystallogr Sect F Struct Biol Cryst Commun</i> <i></i> <i></i> 62: Pt 9 930-934<br/>

http://publicationslist.org/david.aragao/refid8 2011-06-30T00:07:53Z

The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resol...<br/><br/>David Aragão, Sofia Macedo, Edward P Mitchell, Célia V Romão, Ming Y Liu, Carlos Frazão, Lígia M Saraiva, António V Xavier, Jean LeGall, Walter M A M van Dongen, Wilfred R Hagen, Miguel Teixeira, Maria A Carrondo, Peter Lindley (2003) <i>J Biol Inorg Chem</i> <i></i> <i></i> 8: 5 540-548<br/>

http://publicationslist.org/david.aragao/refid7 2011-06-30T00:07:53Z

The structure of dimeric cytochrome c(3) from the sulfate-reducing bacterium Desulfovibrio gigas, diDg, obtained by ab initio methods was further refined to 1.2 A resolution, giving final reliability factors of R(free) = 14.8% and R = 12.4%. This cytochrome is a dimer of tetraheme cytochrome c(3) molecules covalently linked by two solvent-accessible disulfide bridges, a characteristic unique to me...<br/><br/>David Aragão, Carlos Frazão, Larry Sieker, George M Sheldrick, Jean LeGall, Maria Arménia Carrondo (2003) <i>Acta Crystallogr D Biol Crystallogr</i> <i></i> <i></i> 59: Pt 4 644-653<br/>

http://publicationslist.org/david.aragao/refid9 2011-06-30T00:07:53Z

The hybrid cluster protein (HCP) from the sulfate-reducing bacteria Desulfovibrio desulfuricans ATCC 27774 has been isolated and crystallized anaerobically. The protein sample used in the crystallization studies was several months old, having been stored at 193 K, and initial crystal structure studies were unable to fully resolve details of the hybrid cluster despite the use of high-resolution dat...<br/><br/>Sofia Macedo, David Aragão, Edward P Mitchell, Peter Lindley (2003) <i>Acta Crystallogr D Biol Crystallogr</i> <i></i> <i></i> 59: Pt 12 2065-2071<br/>