http://publicationslist.org/data/david.lembo/atom.xml David Lembohttp://publicationslist.org/david.lembo$basepathfavicon.icoRecent additions to David Lembo's PublicationsList.org pagehttp://publicationslist.org/publications.png2012-11-20T11:08:31Z http://publicationslist.org/david.lembo/refid53 2012-11-20T11:02:46Z

The activity of antivirals can be enhanced by their incorporation in nanoparticulate delivery systems. Peculiar polymeric nanoparticles, based on a β-cyclodextrin-poly(4-acryloylmorpholine) monoconjugate (β-CD-PACM), are proposed as acyclovir carriers. The experimental procedure necessary to obtain the acyclovir-loaded nanoparticles using the solvent displacement preparation method will be descr...<br/><br/>Roberta Cavalli, Manuela Donalisio, Agnese Bisazza, Andrea Civra, Elisabetta Ranucci, Paolo Ferruti, David Lembo (2012) <i>Methods Enzymol</i> <i></i> <i></i> 509: 1-19<br/>

http://publicationslist.org/david.lembo/refid51 2012-11-20T11:02:46Z

Respiratory syncytial virus (RSV) interacts with cell surface heparan sulfate proteoglycans (HSPGs) to initiate infection. The interaction of RSV with HSPGs thus presents an attractive target for the development of novel inhibitors of RSV infection. In the present study, a minilibrary of linear, dimeric, and dendrimeric peptides containing clusters of basic amino acids was screened with the aim of...<br/><br/>Manuela Donalisio, Marco Rusnati, Valeria Cagno, Andrea Civra, Antonella Bugatti, Andrea Giuliani, Giovanna Pirri, Marco Volante, Mauro Papotti, Santo Landolfo, David Lembo (2012) <i>Antimicrob Agents Chemother</i> <i></i> <i></i> 56: 10 5278-5288<br/>

http://publicationslist.org/david.lembo/refid52 2012-11-20T11:02:46Z

The development of nonviral gene delivery systems is one of the most intriguing topics in nanomedicine. However, despite the advances made in recent years, several key issues remain unsettled. One of the main problems relates to the difficulty in designing nanodevices for targeted delivery of genes and other drugs to specific anatomic sites. In this study, we describe the development of a novel ch...<br/><br/>Roberta Cavalli, Agnese Bisazza, Michele Trotta, Monica Argenziano, Andrea Civra, Manuela Donalisio, David Lembo (2012) <i>Int J Nanomedicine</i> <i></i> <i></i> 7: 3309-3318<br/>

http://publicationslist.org/david.lembo/refid55 2012-11-20T11:07:39Z

Bisazza, A., Civra, A., Donalisio, M., Lembo, D., Cavalli, R. (2011) <i>Soft matter</i> 7: 22 10590-10593<br/>

http://publicationslist.org/david.lembo/refid48 2011-07-20T14:12:23Z

Human cytomegalovirus (HCMV) infection is associated with severe morbidity and mortality in immunocompromised individuals, mainly transplant recipients and AIDS patients, and is the most frequent cause of congenital malformations in newborn children. To date, few drugs are licensed for the treatment of HCMV infections, most of which target the viral DNA polymerase and suffer from many drawbacks, i...<br/><br/>Beatrice Mercorelli, David Lembo, Giorgio Palù, Arianna Loregian (2011) <i>Pharmacol Ther</i> <i></i> <i></i> 131: 3 309-329<br/>

http://publicationslist.org/david.lembo/refid54 2012-11-20T11:02:46Z

A new poly(amidoamine)-cholesterol (PAA-cholesterol) conjugate was synthesized, characterized and used to produce nanoparticles by the electrospraying technique. The electrospraying is a method of liquid atomization that consists in the dispersion of a solution into small charged droplets by an electric field. Tuning the electrospraying process parameters spherical PAA-chol nanoparticles formed. T...<br/><br/>R Cavalli, A Bisazza, R Bussano, M Trotta, A Civra, D Lembo, E Ranucci, P Ferruti (2011) <i>J Drug Deliv</i> <i></i> <i></i> 2011: <br/>

http://publicationslist.org/david.lembo/refid50 2011-07-20T14:12:23Z

Six recently synthesized cyano-substituted heteroaryles, which do not bind to DNA but are highly cytotoxic against the human tumor cell line HeLa, were analyzed for their antitumor mechanisms of action (MOA). They did not interfere with the expression of human papillomavirus oncogenes integrated in the HeLa cell genome, but they did induce strong G1 arrest and result in the activation of caspase-3...<br/><br/>Katja Ester, Fran Supek, Kristina Majsec, Marko Marjanović, David Lembo, Manuela Donalisio, Tomislav Smuc, Ivana Jarak, Grace Karminski-Zamola, Marijeta Kralj (2010) <i>Invest New Drugs</i> <i></i> <i></i> : <br/>

http://publicationslist.org/david.lembo/refid43 2010-09-21T09:57:40Z

To date, there are few drugs licensed for the treatment of HCMV infections, most of which target the viral DNA polymerase and suffer from many drawbacks. Thus, there is still a strong need for new anti-HCMV compounds with novel mechanisms of action. In this study, we investigated the anti-HCMV activity of chemically sulfated derivatives of Escherichia coli K5 capsular polysaccharide. These compoun...<br/><br/>Beatrice Mercorelli, Pasqua Oreste, Elisa Sinigalia, Giulia Muratore, David Lembo, Giorgio Palù, Arianna Loregian (2010) <i>Antimicrob Agents Chemother</i> <i></i> <i></i> : <br/>

http://publicationslist.org/david.lembo/refid45 2010-09-21T09:57:40Z

Cytokines play an important role in the control of mucosal HPV transcription. However, there is little data available on cutaneous HPVs, which are associated with non-melanoma skin cancers. Here, we describe a cell-based assay exploiting HaCaT keratinocytes stably transfected with a reporter construct containing the long control region (LCR) regulatory sequence of gene transcription in HPV-5 and H...<br/><br/>Manuela Donalisio, Alice Poli, Andrea Civra, Santo Landolfo, David Lembo (2010) <i>Arch Virol</i> <i></i> <i></i> 155: 4 583-587<br/>

http://publicationslist.org/david.lembo/refid44 2010-09-21T09:57:40Z

Peptide dendrimers consist of a peptidyl branching core and/or covalently attached surface functional units. They show a variety of biological properties, including antiviral activity. In this study, a minilibrary of linear, dimeric, and dendrimeric peptides containing clusters of basic amino acids was evaluated for in vitro activity against human papillomaviruses (HPVs). The peptide dendrimer SB1...<br/><br/>Manuela Donalisio, Marco Rusnati, Andrea Civra, Antonella Bugatti, Donatella Allemand, Giovanna Pirri, Andrea Giuliani, Santo Landolfo, David Lembo (2010) <i>Antimicrob Agents Chemother</i> <i></i> <i></i> 54: 10 4290-4299<br/>

http://publicationslist.org/david.lembo/refid49 2011-07-20T14:12:23Z

Nanomedicine opens new therapeutic avenues for attacking viral diseases and for improving treatment success rates. Nanoparticulate-based systems might change the release kinetics of antivirals, increase their bioavailability, improve their efficacy, restrict adverse drug side effects and reduce treatment costs. Moreover, they could permit the delivery of antiviral drugs to specific target sites an...<br/><br/>David Lembo, Roberta Cavalli (2010) <i>Antivir Chem Chemother</i> <i></i> <i></i> 21: 2 53-70<br/>

http://publicationslist.org/david.lembo/refid2 2010-01-11T12:33:08Z

Ribonucleotide reductase (RNR), a crucial enzyme for nucleotide anabolism, is encoded by all living organisms and by large DNA viruses such as the herpesviruses. Surprisingly, the beta-herpesvirus subfamily RNR R1 subunit homologues are catalytically inactive and their function remained enigmatic for many years. Recent work sheds light on the function of M45, the murine cytomegalovirus R1 homologu...<br/><br/>David Lembo, Wolfram Brune (2009) <i>Trends Biochem Sci</i> <i></i> <i></i> 34: 1 25-32<br/>

http://publicationslist.org/david.lembo/refid41 2010-01-11T12:33:08Z

Antiviral microbicides, topical agents that prevent sexually transmitted infections, mainly work by blocking the interaction between viral proteins and cell surface components. In many instances, virus-cell interaction is mediated by cell surface heparan sulfate proteoglycans (HSPGs). HSPGs are exploited as attachment receptors by three sexually transmitted viruses: Human Immunodeficiency Virus (H...<br/><br/>Marco Rusnati, Elisa Vicenzi, Manuela Donalisio, Pasqua Oreste, Santo Landolfo, David Lembo (2009) <i>Pharmacol Ther</i> <i></i> <i></i> 123: 3 310-322<br/>

http://publicationslist.org/david.lembo/refid42 2010-09-21T09:57:40Z

Dextran nanobubbles were prepared with a dextran shell and a perfluoropentan core in which oxygen was stored. To increase the stability polyvinylpirrolidone was also added to the formulation as stabilizing agent. Rhodamine B was used as fluorescent marker to obtain fluorescent nanobubbles. The nanobubble formulations showed sizes of about 500nm, a negative surface charge and a good capacity of loa...<br/><br/>R Cavalli, A Bisazza, P Giustetto, A Civra, D Lembo, G Trotta, C Guiot, M Trotta (2009) <i>Int J Pharm</i> <i></i> <i></i> 381: 2 160-165<br/>

http://publicationslist.org/david.lembo/refid1 2010-01-11T12:33:08Z

Novel polymeric nanoparticles based on a beta-cyclodextrin-poly(4-acryloylmorpholine) mono-conjugate (beta-CD-PACM), a tadpole-shaped polymer in which the beta-CD ring is the hydrophilic head and the PACM chain the amphiphilic tail, were prepared by the solvent injection technique. Acyclovir-loaded nanoparticles were prepared from inclusion complexes of Acyclovir with beta-CD-PACM. Both unloaded a...<br/><br/>Roberta Cavalli, Manuela Donalisio, Andrea Civra, Paolo Ferruti, Elisabetta Ranucci, Francesco Trotta, David Lembo (2009) <i>J Control Release</i> <i></i> <i></i> 137: 2 116-122<br/>

http://publicationslist.org/david.lembo/refid5 2009-05-12T10:16:09Z

Host immune response to human papillomavirus (HPV) is a crucial factor in viral clearance and control of persistent infections. The existence of an intercellular control mechanism mediated by cytokines to suppress HPV-gene transcription and to prevent malignant conversion of HPV-infected cells, has been postulated. In a previous study, we demonstrated the inhibitory activity of several cytokines o...<br/><br/>Manuela Donalisio, Maura Cornaglia, Santo Landolfo, David Lembo (2008) <i>Virus Res</i> <i></i> <i></i> 132: 1-2 253-256<br/>

http://publicationslist.org/david.lembo/refid6 2009-05-12T10:16:09Z

A poly(amidoamine) (PAA) copolymer with beta-cyclodextrin was obtained by polyaddition reaction of 6-deoxy-6-amino-beta-cyclodextrin (beta-CD-NH(2)) and 2-methylpiperazine to 2,2-bis(acrylamido)acetic acid in aqueous medium. This beta-CD/PAA copolymer bears beta-CD units along the macromolecular chain, is water-soluble and non-cytotoxic. The complexing capacity of beta-CD/PAA was determined using ...<br/><br/>Marco Bencini, Elisabetta Ranucci, Paolo Ferruti, Francesco Trotta, Manuela Donalisio, Maura Cornaglia, David Lembo, Roberta Cavalli (2008) <i>J Control Release</i> <i></i> <i></i> 126: 1 17-25<br/>

http://publicationslist.org/david.lembo/refid4 2009-05-12T10:16:09Z

Genital human papillomaviruses (HPV) represent the most common sexually transmitted agents and are classified into low or high risk by their propensity to cause genital warts or cervical cancer, respectively. Topical microbicides against HPV may be a useful adjunct to the newly licensed HPV vaccine. A main objective in the development of novel microbicides is to block HPV entry into epithelial cel...<br/><br/>David Lembo, Manuela Donalisio, Marco Rusnati, Antonella Bugatti, Maura Cornaglia, Paola Cappello, Mirella Giovarelli, Pasqua Oreste, Santo Landolfo (2008) <i>Antimicrob Agents Chemother</i> <i></i> <i></i> 52: 4 1374-1381<br/>

http://publicationslist.org/david.lembo/refid3 2009-05-12T10:16:09Z

TNFalpha is an important cytokine in antimicrobial immunity and inflammation. The receptor-interacting protein RIP1 is an essential component of the TNF receptor 1 signaling pathway that mediates the activation of NF-kappaB, MAPKs, and programmed cell death. It also transduces signals derived from Toll-like receptors and intracellular sensors of DNA damage and double-stranded RNA. Here, we show th...<br/><br/>Claudia Mack, Albert Sickmann, David Lembo, Wolfram Brune (2008) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 105: 8 3094-3099<br/>

http://publicationslist.org/david.lembo/refid7 2009-05-12T10:16:09Z

The p53R2 protein is a p53-inducible small subunit of ribonucleotide reductase. It plays a crucial role in p53-dependent cellular response to DNA damage and oxidative stress by providing deoxyribonucleotides (dNTPs) to the DNA repair machinery and by scavenging reactive oxygen species (ROS). To investigate the effects of high-risk human papillomavirus (HPV) oncoproteins on p53R2 expression after D...<br/><br/>David Lembo, Manuela Donalisio, Maura Cornaglia, Barbara Azzimonti, Anna Demurtas, Santo Landolfo (2006) <i>Virus Res</i> <i></i> <i></i> 122: 1-2 189-193<br/>

http://publicationslist.org/david.lembo/refid8 2009-05-12T10:16:09Z

Cervical carcinomas express human papillomavirus (HPV) E6 and E7 oncoproteins, which are required to maintain the proliferative state of cancer cells. Repression of E6 and E7 expression results in acquisition of senescent phenotype and in the rescue of functional p53 and p105(Rb) pathways; therefore, therapies directed against either viral protein may be beneficial. However, the systems to study H...<br/><br/>David Lembo, Manuela Donalisio, Marco De Andrea, Maura Cornaglia, Sara Scutera, Tiziana Musso, Santo Landolfo (2006) <i>FASEB J</i> <i></i> <i></i> 20: 1 148-150<br/>

http://publicationslist.org/david.lembo/refid9 2009-05-12T10:16:09Z

Ribonucleotide reductase (RNR) is the key enzyme in the biosynthesis of deoxyribonucleotides. Alpha- and gammaherpesviruses express a functional enzyme, since they code for both the R1 and the R2 subunits. By contrast, betaherpesviruses contain an open reading frame (ORF) with homology to R1, but an ORF for R2 is absent, suggesting that they do not express a functional RNR. The M45 protein of muri...<br/><br/>David Lembo, Manuela Donalisio, Anders Hofer, Maura Cornaglia, Wolfram Brune, Ulrich Koszinowski, Lars Thelander, Santo Landolfo (2004) <i>J Virol</i> <i></i> <i></i> 78: 8 4278-4288<br/>

http://publicationslist.org/david.lembo/refid11 2009-05-12T10:16:09Z

BACKGROUND: B19 virus infection with persistent anaemia has been reported in organ transplant recipients. Detection of B19 virus DNA in serum is the best direct marker of active infection. OBJECTIVE: The present study evaluated the incidence and clinical role of active B19 virus infection in renal transplant recipients presenting with anaemia. STUDY DESIGN: Forty-eight such recipients were investi...<br/><br/>Rossana Cavallo, C Merlino, D Re, C Bollero, M Bergallo, D Lembo, T Musso, G Leonardi, G P Segoloni, A Negro Ponzi (2003) <i>J Clin Virol</i> <i></i> <i></i> 26: 3 361-368<br/>

http://publicationslist.org/david.lembo/refid10 2009-05-12T10:16:09Z

Human cytomegalovirus (HCMV), a betaherpesvirus, represents the major infectious cause of birth defects, as well as an important pathogen for immunocompromised individuals. The viral nucleocapsid containing a linear double-stranded DNA of 230 kb is surrounded by a proteinaceous tegument, which is itself enclosed by a loosely applied lipid bilayer. Expression of the HCMV genome is controlled by a c...<br/><br/>Santo Landolfo, Marisa Gariglio, Giorgio Gribaudo, David Lembo (2003) <i>Pharmacol Ther</i> <i></i> <i></i> 98: 3 269-297<br/>

http://publicationslist.org/david.lembo/refid12 2009-05-12T10:16:09Z

Prions result in fatal degeneration of the central nervous system (CNS) in the form of diseases known as transmissible spongiform encephalopathies (TSEs). The discovery in 1996 of a new variant of Creutzfeldt-Jakob disease (a human TSE) and experimental confirmation that it is caused by the prion strain responsible for bovine spongiform encephalopathy (BSE) has greatly spurred research in this fie...<br/><br/>Giorgio Cavallo, David Lembo, Rossana Cavallo (2002) <i>Riv Biol</i> <i></i> <i></i> 95: 2 211-238<br/>

http://publicationslist.org/david.lembo/refid13 2009-05-12T10:16:09Z

Cytomegalovirus (CMV) infection stimulates the expression of cellular enzymes involved in the biosynthesis of DNA precursors. Among them, dihydrofolate reductase (DHFR) and thymidylate synthase (TS) require folate as coenzymes. In growing cells, folates are readily converted to polyglutamated forms by the cellular enzyme folylpolyglutamate synthetase (FPGS). Polyglutamated folates are selectively ...<br/><br/>R Cavallo, D Lembo, G Gribaudo, S Landolfo (2001) <i>Intervirology</i> <i></i> <i></i> 44: 4 224-226<br/>

http://publicationslist.org/david.lembo/refid15 2009-05-12T10:16:09Z

Cytomegalovirus (CMV) replication in non-proliferating cells requires the coordinated expression of the host enzymes responsible for deoxyribonucleotide synthesis. Thymidylate synthase (TS) is an essential cellular enzyme that catalyzes de novo synthesis of thymidylic acid (dTMP). In this report we show that murine CMV (MCMV) replication and DNA synthesis are inhibited in quiescent 3T6 fibroblasts...<br/><br/>G Gribaudo, L Riera, D Lembo, M De Andrea, L F Johnson, S Landolfo (2001) <i>Virus Res</i> <i></i> <i></i> 73: 1 57-65<br/>

http://publicationslist.org/david.lembo/refid14 2009-05-12T10:16:09Z

Infection of cells with viable or UV-inactivated murine cytomegalovirus (MCMV) increased the IFN-inducible 204 gene at both the mRNA and the protein levels. The activity of a reporter gene driven by the mouse Ifi204 promoter induced following virus infection showed that this increase was due to transcriptional activation. Moreover, FACS analysis of infected mouse embryo fibroblasts (MEF) stably tr...<br/><br/>S Rolle, M De Andrea, D Gioia, D Lembo, L Hertel, S Landolfo, M Gariglio (2001) <i>Virology</i> <i></i> <i></i> 286: 2 249-255<br/>

http://publicationslist.org/david.lembo/refid16 2009-05-12T10:16:09Z

Ribonucleotide reductase (RNR) is an essential enzyme for the de novo synthesis of both cellular and viral DNA and catalyzes the conversion of ribonucleoside diphosphates into the corresponding deoxyribonucleoside diphosphates. The enzyme consists of two nonidentical subunits, termed R1 and R2, whose expression is very low in resting cells and maximal in S-phase cells. Here we show that murine cyt...<br/><br/>D Lembo, G Gribaudo, A Hofer, L Riera, M Cornaglia, A Mondo, A Angeretti, M Gariglio, L Thelander, S Landolfo (2000) <i>J Virol</i> <i></i> <i></i> 74: 24 11557-11565<br/>

http://publicationslist.org/david.lembo/refid18 2009-05-12T10:16:09Z

Herpesviruses accomplish DNA replication either by expressing their own deoxyribonucleotide biosynthetic genes or by stimulating the expression of the corresponding cellular genes. Cytomegalovirus (CMV) has adopted the latter strategy to allow efficient replication in quiescent cells. In the present report, we show that murine CMV (MCMV) infection of quiescent fibroblasts induces both mRNA and pro...<br/><br/>G Gribaudo, L Riera, D Lembo, M De Andrea, M Gariglio, T L Rudge, L F Johnson, S Landolfo (2000) <i>J Virol</i> <i></i> <i></i> 74: 11 4979-4987<br/>

http://publicationslist.org/david.lembo/refid17 2009-05-12T10:16:09Z

Tomudex (ZD1694) is a quinazoline-based folate analog and a powerful inhibitor of cellular thymidylate synthase and is approved in Europe for use in oncology. Here the first evidence of its activity against murine and human cytomegalovirus (MCMV and HCMV) is reported. ZD1694 irreversibly inhibited the replication and DNA synthesis of both viruses in quiescent fibroblasts. The corresponding 50% eff...<br/><br/>D Lembo, G Gribaudo, L Riera, A Mondo, R Cavallo, A Angeretti, S Landolfo (2000) <i>Antiviral Res</i> <i></i> <i></i> 47: 2 111-120<br/>

http://publicationslist.org/david.lembo/refid19 2009-05-12T10:16:09Z

Cytomegalovirus (CMV) stimulates numerous cellular pathways upon infection. One of these pathways involves activation of dihydrofolate reductase (DHFR), an essential enzyme in the biosynthesis of purines and thymidylate. Here we report that methotrexate (MTX), an inhibitor of DHFR, suppresses murine CMV replication at the level of DNA synthesis in quiescent NIH 3T3 cells. However, MTX has no antiv...<br/><br/>D Lembo, R Cavallo, M Cornaglia, A Mondo, L Hertel, A Angeretti, S Landolfo (1999) <i>Arch Virol</i> <i></i> <i></i> 144: 7 1397-1403<br/>

http://publicationslist.org/david.lembo/refid20 2009-05-12T10:16:09Z

Human cytomegalovirus (HCMV) productively infects quiescent fibroblasts in which the levels of deoxynucleotide triphosphates (dNTPs) and cell functions involved in DNA metabolism are very low. Since sufficient dNTPs levels are essential for human HCMV replication, host cell enzymes involved in the biosynthesis of dNTPs might be expected to be stimulated by viral infection in quiescent cells. We re...<br/><br/>D Lembo, G Gribaudo, R Cavallo, L Riera, A Angeretti, L Hertel, S Landolfo (1999) <i>Intervirology</i> <i></i> <i></i> 42: 1 30-36<br/>

http://publicationslist.org/david.lembo/refid22 2009-05-12T10:16:09Z

Murine cytomegalovirus (MCMV) productively infects quiescent fibroblasts in which the levels of nucleoside triphosphate precursors and cell functions involved in DNA metabolism are minimal. It appears that MCMV has evolved molecular pathways in order to ensure the presence of nucleoside triphosphate precursors for the viral DNA polymerase. Here, we report that MCMV infection of quiescent NIH 3T3 c...<br/><br/>D Lembo, A Angeretti, M Gariglio, S Landolfo (1998) <i>J Gen Virol</i> <i></i> <i></i> 79 ( Pt 11): 2803-2807<br/>

http://publicationslist.org/david.lembo/refid21 2009-05-12T10:16:09Z

The biological activities of interferons (IFNs) are mediated by IFN-induced proteins. One family is encoded by several structurally related genes located on murine chromosome 1 (Ifi 200 cluster) and three homologous genes (MNDA, IFI 16 and AIM2) located on human chromosome 1 as well, within a linkage group highly conserved between mouse and human. All the proteins of this family contain at least o...<br/><br/>S Landolfo, M Gariglio, G Gribaudo, D Lembo (1998) <i>Biochimie</i> <i></i> <i></i> 80: 8-9 721-728<br/>

http://publicationslist.org/david.lembo/refid24 2009-05-12T10:16:09Z

A clone containing the gene ifngr2 for the second chain (IFN-gamma R2) of the mouse interferon gamma receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5'-flanking region several transcription initiation sites between 27 and 136 n...<br/><br/>C Ebensperger, S Rhee, G Muthukumaran, D Lembo, R Donnelly, S Pestka, Z Dembic (1996) <i>Scand J Immunol</i> <i></i> <i></i> 44: 6 599-606<br/>

http://publicationslist.org/david.lembo/refid25 2009-05-12T10:16:09Z

C57BL/6 mice are unable to express the Ifi 202 type genes upon injection in vivo of multiple dsRNA, poly rl:rC, or IFN-treatment in vitro. For this purpose the 5' terminal flanking region (called the b segment of 804 bp) was linked to a heterologous reporter gene chloramphenicol acetyl transferase (CAT) and transfected into NIH3T3 cells or BLK cells derived from the C57BL/6 strain. IFN-alpha induc...<br/><br/>M Gariglio, P Foresta, G G Ying, M Gaboli, D Lembo, S Landolfo (1996) <i>J Cell Biochem</i> <i></i> <i></i> 60: 1 83-94<br/>

http://publicationslist.org/david.lembo/refid23 2009-05-12T10:16:09Z

Studies of hamster-human and mouse-human somatic fibroblast hybrids and transfected mouse fibroblasts have demonstrated that signaling through the human interferon-gamma receptor (hu-IFN-gammaR) requires the formation of a complex consisting of ligand (IFN-gamma), a ligand binding receptor chain (IFN-gammaR1), and a signal transducing receptor chain (IFN-gammaR2). To date, the ability of this rece...<br/><br/>D Lembo, P Ricciardi-Castagnoli, G Alber, L Ozmen, S Landolfo, H Blüthmann, Z Dembic, S V Kotenko, J R Cook, S Pestka, G Garotta (1996) <i>J Biol Chem</i> <i></i> <i></i> 271: 51 32659-32666<br/>

http://publicationslist.org/david.lembo/refid26 2009-05-12T10:16:09Z

p202 is a protein expressed in murine cells after Interferon treatment. Although the function of p202 is still basically unknown, its ability to bind the hypophosphorylated form of the retinoblastoma protein pRb suggests a possible role in the control of cell proliferation. To investigate the role of p202 we have generated several cell clones of NIH 3T3 fibroblasts that constitutively express p202...<br/><br/>D Lembo, A Angeretti, S Benefazio, L Hertel, M Gariglio, F Novelli, S Landolfo (1995) <i>J Biol Regul Homeost Agents</i> <i></i> <i></i> 9: 2 42-46<br/>

http://publicationslist.org/david.lembo/refid27 2009-05-12T10:16:09Z

Products of ras oncogenes strongly stimulate the activity of the reporter gene, chloramphenicol acetyltransferase (CAT), driven by a 1.2 kb fragment of the murine cytomegalovirus (MCMV) immediate early (IE) gene enhancer (pCMVCAT). To define the role of proteins binding to the unique cAMP response element (CRE) present in the IE enhancer, NIH 3T3 cells were cotransfected with prasZip6 plasmid, a m...<br/><br/>M Gaboli, A Angeretti, D Lembo, M Gariglio, G Gribaudo, S Landolfo (1995) <i>J Gen Virol</i> <i></i> <i></i> 76 ( Pt 4): 751-758<br/>

http://publicationslist.org/david.lembo/refid28 2009-05-12T10:16:09Z

Cotransfection of NIH 3T3 cells with a mammalian expression vector containing a v-Ha-ras gene, together with a plasmid carrying the human immunodeficiency virus (HIV) long terminal repeat (LTR) linked to the chloramphenicol acetyl transferase (CAT) reporter gene, significantly stimulated CAT activity. High HIV LTR activation was also observed in cell lines carrying stably transfected ras oncogenes...<br/><br/>D Lembo, A Angeretti, M Gaboli, R Cavallo, S Landolfo (1995) <i>New Microbiol</i> <i></i> <i></i> 18: 2 111-116<br/>

http://publicationslist.org/david.lembo/refid31 2009-05-12T10:16:09Z

Diabetes-prone (DP) BB rats spontaneously develop a hyperglycaemic condition which closely resembles human insulin-dependent diabetes mellitus (IDDM), both in terms of clinical and histological features. The incidence of IDDM was significantly reduced when these animals were treated with 2 or 4 mg fusidic acid (FA)/day i.m. from day 30 to day 120 of age. In addition, the mean insulitis score was s...<br/><br/>F Nicoletti, R Di Marco, S Morrone, P Zaccone, D Lembo, S Grasso, A Santoni, P L Meroni, K Bendtzen (1994) <i>Immunology</i> <i></i> <i></i> 81: 2 317-321<br/>

http://publicationslist.org/david.lembo/refid29 2009-05-12T10:16:09Z

When treated with IFN-alpha, L1210 leukemia cells express high levels of the mouse 202 gene mRNA after a few hours. Three tandem copies of a 43 bp fragment (GAbox) homologous to the IFN-stimulatable response element (ISRE), located in the 5'-flanking region of the 202 gene, were linked to the reporter CAT gene and transiently transfected into L1210 cells. The data suggest that the GA box is suffic...<br/><br/>M Gariglio, M Gaboli, C Mana, P Foresta, G G Ying, A Angeretti, D Lembo, S Landolfo (1994) <i>New Microbiol</i> <i></i> <i></i> 17: 4 259-267<br/>

http://publicationslist.org/david.lembo/refid30 2009-05-12T10:16:09Z

The ras gene family encodes 21K proteins that reside on the inner face of the plasma membrane and bind GTP and GDP with an equally high affinity. Cotransfection of NIH 3T3 cells with a mammalian expression vector containing a viral Harvey-ras (v-Ha-ras) cDNA, together with a plasmid (pCMVCAT) carrying the immediate early (IE) enhancer of the murine cytomegalovirus (MCMV) linked to the chlorampheni...<br/><br/>D Lembo, A Angeretti, P Foresta, G Gribaudo, M Gariglio, S Landolfo (1994) <i>J Gen Virol</i> <i></i> <i></i> 75 ( Pt 7): 1685-1692<br/>

http://publicationslist.org/david.lembo/refid32 2009-05-12T10:16:09Z

Interferon-alpha (IFN-alpha) significantly reduced the replication of murine cytomegalovirus (MCMV) in mouse embryo fibroblasts derived from the susceptible mouse strain C3H/HeJ. When infectious virus production was measured, a strong decrease in virus titer was observed in IFN-treated cells at a multiplicity of infection (moi) of 1 and 0.5 pfu/cell. Analysis of virus-specified mRNAs by Northern b...<br/><br/>M G Martinotti, G Gribaudo, M Gariglio, A Caliendo, D Lembo, A Angeretti, R Cavallo, S Landolfo (1993) <i>J Interferon Res</i> <i></i> <i></i> 13: 2 105-109<br/>

http://publicationslist.org/david.lembo/refid33 2009-05-12T10:16:09Z

In the present study, a pre-B cell leukemia L1210-C7, representing a very early stage of the B lineage, was used to characterize the molecular mechanisms exploited by IFN-gamma to modulate B cell activity. A cDNA library was prepared with poly (A)+ RNA from cells stimulated with IFN-gamma and three cDNAs clones complementary to IFN-gamma inducible mRNAs were isolated by differential screening. Of ...<br/><br/>G Gribaudo, A Caliendo, D Lembo, R Cavallo, S Landolfo (1992) <i>J Biol Regul Homeost Agents</i> <i></i> <i></i> 6: 4 137-141<br/>

http://publicationslist.org/david.lembo/refid35 2009-05-12T10:16:09Z

MuIFN-gamma receptor cDNA has been stably transfected in the human WISH cell line. A stable transfectant, denominated WISH-C2, expressed 46;000 receptors/cell whose affinity was similar to that observed on murine cells (Kd = 2.6 x 10(-9) M). When WISH-C2 cells were treated with Hu- or MuIFN-gamma, antiviral and antiproliferative activities were observed with HuIFN-gamma only. These findings sugges...<br/><br/>D Lembo, F Cofano, G Cavallo, S Landolfo (1992) <i>J Biol Regul Homeost Agents</i> <i></i> <i></i> 6: 1 26-30<br/>

http://publicationslist.org/david.lembo/refid34 2009-05-12T10:16:09Z

Type I and II interferons (IFNs) stimulate the expression of the 202 and 2'-5' oligoadenylate synthetase (OASE) genes in L929, NIH 3T3 and LM-TK- fibroblastic cell lines. In two other cell lines, B16 melanoma and F9 teratocarcinoma, these cytokines induce OASE but not the 202 mRNA. In L929 cells, IFN-alpha induces the 202 mRNA at concentrations between 10 and 10(3) units/ml. To achieve maximal ind...<br/><br/>D Lembo, M Gaboli, A Caliendo, F Falciani, E Garattini, S Landolfo (1992) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 187: 2 628-634<br/>

http://publicationslist.org/david.lembo/refid36 2009-05-12T10:16:09Z

The 40-kDa 2'-5'-oligoadenylate [(2'-5') (A)n] synthetase isoenzyme was proven to be a mediator of the inhibition of encephalomyocarditis virus (EMCV) replication by interferon (IFN). When activated by double-stranded RNA, this enzyme converts ATP into 2'-5'-oligoadenylate [(2'-5') (A)n], and (2'-5') (A)n was found to accumulate in IFN-treated, EMCV-infected cells. The only known function of (2'-5...<br/><br/>G Gribaudo, D Lembo, G Cavallo, S Landolfo, P Lengyel (1991) <i>J Virol</i> <i></i> <i></i> 65: 4 1748-1757<br/>

http://publicationslist.org/david.lembo/refid37 2009-05-12T10:16:09Z

G Gribaudo, A Franco, D Lembo, G Cavallo, S Landolfo (1990) <i>Haematologica</i> <i></i> <i></i> 75 Suppl 4: 3-9<br/>

http://publicationslist.org/david.lembo/refid47 2010-09-21T09:55:54Z

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