http://publicationslist.org/data/david.wade/atom.xml David Wadehttp://publicationslist.org/david.wade$basepathfavicon.icoRecent additions to David Wade's PublicationsList.org pagehttp://publicationslist.org/publications.png2010-07-07T23:54:15Z http://publicationslist.org/david.wade/refid67 2010-07-07T23:51:58Z

This article proposes the synthesis and study of a peptide that is composed of 13 amino acids, and that has an amino acid composition and sequence that can be described with International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology, Joint Commission on Biochemical Nomenclature single letter abbreviations corresponding to the sequence of letters in ...<br/><br/>David Wade (2010) <i>Wade Research Foundation Reports</i> 5: 3 1-15<br/>

http://publicationslist.org/david.wade/refid65 2010-07-07T23:53:23Z

The Temporin-/Vespid Chemotactic Peptide- (or T/V-) like peptides are a group of small [average of 13 amino acids (AAs)], linear (no Cysteine), hydrophobic (avg. 65% hydrophobic AAs), positively charged (avg. net charge at pH 7 = +2), structurally very similar, peptide amides that have been isolated from the skins of frogs and the venoms of insects during the past 30 years. Recently, the number of...<br/><br/>David Wade (2010) <i>Wade Research Foundation Reports</i> 5: 1 1<br/>

http://publicationslist.org/david.wade/refid66 2010-07-07T23:48:26Z

The name-to-peptide concept uses the International Union of Pure and Applied Chemistry-International Union of Biochemistry and Molecular Biology, Joint Commission on Biochemical Nomenclature (IUPAC-IUB, JCBN) system for abbreviating the names of amino acids (AAs) with single letters of the English alphabet, and considers the strings of letters in personal and other names as strings of AAs, or pept...<br/><br/>David Wade (2010) <i>Wade Research Foundation Reports</i> 5: 2 1-23<br/>

http://publicationslist.org/david.wade/refid18 2009-12-09T06:16:29Z

Three of the most recognizable corporate names are Microsoft, a manufacturer of computer software, and Google and Yahoo, two internet search providers. Two of the most popular soft drink brand names are Coca-Cola and Pepsi-Cola. This article proposes to use the sequences of English alphabet letters in the names of these companies and products as the basis for creating five new peptides, MICROSOFT,...<br/><br/>David Wade (2007) <i>Wade Research Foundation Reports</i> 4: 1 1-16<br/>

http://publicationslist.org/david.wade/refid16 2009-12-09T06:03:51Z

This article is a revised version of an article with the same title that was published by the author in 2006, and it corrects errors that are present in the previous version. A method is presented for determining the energy of a name as the acoustical energy of the vocalized name. The names, Madonna and Esther, as spoken by the author and by a computerized text-to-speech program, were used to tes...<br/><br/>David Wade (2007) <i>Wade Research Foundation Reports</i> 4: 3 1-14<br/>

http://publicationslist.org/david.wade/refid17 2009-12-09T06:09:18Z

This article describes a theoretical experiment that uses the International Union of Pure and Applied Chemistry’s one letter system for abbreviating the chemical names of amino acids to interpret the letter sequences, YHWH and YHVH, English transliterations of the Hebrew name of God, as representing the amino acid sequences of two tetrapeptides, and proposes the chemical synthesis and biological...<br/><br/>David Wade (2007) <i>Wade Research Foundation Reports</i> 4: 2 1-6<br/>

http://publicationslist.org/david.wade/refid13 2009-12-09T05:44:29Z

What is the basis of the preference for one given, or first, name versus another? This article explores that question by analyzing the acoustic, or sound, energy of vocalized names from the US Social Security Administration’s list of the thousand most popular given names for the period of 2000-2005. The 100 most popular, and 100 least popular, male and female given names were vocalized by a comp...<br/><br/>David Wade (2007) <i>Wade Research Foundation Reports</i> 4: 6 1-29<br/>

http://publicationslist.org/david.wade/refid14 2009-12-09T05:43:37Z

This article proposes the chemical synthesis and study of a peptide that has an amino acid (AA) sequence with one letter abbreviations that correspond to the name, KAROLINSKA: Lysine (K)-Alanine (A)-Arginine (R)-Ornithine (O)-Leucine (L)-Isoleucine (I)-Asparagine (N)-Serine (S)-Lysine (K)-Alanine (A). In this sequence, nine of the ten AAs are represented by official International Union of Pure and...<br/><br/>David Wade (2007) <i>Wade Research Foundation Reports</i> 4: 5 1-7<br/>

http://publicationslist.org/david.wade/refid15 2009-12-09T05:48:18Z

This article proposes the chemical synthesis and study of a peptide that has an amino acid (AA) sequence with one letter abbreviations that correspond to the name, PRINCETON: Proline (P)-Arginine (R)-Isoleucine (I)-Asparagine (N)-Cysteine (C)-Glutamic acid (E)-Threonine (T)- Ornithine (O)-Asparagine (N). In this sequence, eight of the nine AAs are represented by official International Union of Pur...<br/><br/>David Wade (2007) <i>Wade Research Foundation Reports</i> 4: 4 1-8<br/>

http://publicationslist.org/david.wade/refid19 2009-12-09T06:19:58Z

A method is presented for determining the energy of a name as the acoustic energy of the spoken name. The names, Madonna and Esther, as spoken by the author, differed by ~0.5 V of acoustic energy. This evidence may support the hypothesis of a difference in the energies of names.<br/><br/>David Wade (2006) <i>Wade Research Foundation Reports</i> 2: 1-3 <br/>

http://publicationslist.org/david.wade/refid21 2009-12-09T06:40:04Z

The ability of nisin, synthetic temporin analogs, magainins, defensins, and cecropins to inhibit Bacillus anthracis, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, and Bacillus subtilis growth from spore inocula was determined using well diffusion assays. Nisin, magainin II amide, and defensins were inhibitory in screening against B. anthracis Sterne or B. cereus ATCC 7004, but only n...<br/><br/>Thomas J Montville, Tara De Siano, Adam Nock, Sally Padhi, David Wade (2006) <i>J Food Prot</i> 69: 10 2529-2533<br/>

http://publicationslist.org/david.wade/refid1 2009-12-09T05:04:37Z

Topical antimicrobicides hold great promise in reducing human immunodeficiency virus (HIV) transmission. Amphibian skin provides a rich source of broad-spectrum antimicrobial peptides including some that have antiviral activity. We tested 14 peptides derived from diverse amphibian species for the capacity to inhibit HIV infection. Three peptides (caerin 1.1, caerin 1.9, and maculatin 1.1) complete...<br/><br/>Scott E VanCompernolle, R Jeffery Taylor, Kyra Oswald-Richter, Jiyang Jiang, Bryan E Youree, John H Bowie, Michael J Tyler, J Michael Conlon, David Wade, Christopher Aiken, Terence S Dermody, Vineet N KewalRamani, Louise A Rollins-Smith, Derya Unutmaz (2005) <i>J Virol</i> 79: 18 11598-11606<br/>

http://publicationslist.org/david.wade/refid22 2009-12-09T06:55:12Z

The ability of five purified amphibian antimicrobial peptides (dermaseptin-1, temporin A, magainin I, and II, PGLa), crude peptide fractions isolated from the skin of Rana pipiens and R. catesbeiana, and four antimicrobial peptides (AMPs) from hybrid striped bass (piscidin-1N, -1H, -2, and -3) were examined for their ability to reduce the infectivity of channel catfish virus (CCV) and frog virus 3...<br/><br/>V G Chinchar, L Bryan, U Silphadaung, E Noga, D Wade, L Rollins-Smith (2004) <i>Virology</i> 323: 2 268-275<br/>

http://publicationslist.org/david.wade/refid2 2009-12-09T05:07:46Z

Many mammalian antimicrobial peptides (AMPs) have multiple effects on antimicrobial immunity. We found that temporin A (TA), a representative frog-derived AMP, induced the migration of human monocytes, neutrophils, and macrophages with a bell-shaped response curve in a pertussis toxin-sensitive manner, activated p44/42 MAPK, and stimulated Ca(2+) flux in monocytes, suggesting that TA is capable of...<br/><br/>Qian Chen, David Wade, Kahori Kurosaka, Zhao Yuan Wang, Joost J Oppenheim, De Yang (2004) <i>J Immunol</i> 173: 4 2652-2659<br/>

http://publicationslist.org/david.wade/refid63 2009-12-10T09:36:39Z

D. Wade, J. Englund (2004) <i>Peptide Revolution: Genomics, Proteomics & Therapeutics</i> 881-882<br/>

http://publicationslist.org/david.wade/refid64 2009-12-10T09:38:57Z

D. Wade (2004) <i>Peptide Revolution: Genomics, Proteomics & Therapeutics</i> 949-951<br/>

http://publicationslist.org/david.wade/refid20 2009-12-09T06:27:32Z

A peptide was designed so that the one letter abbreviations for its amino acid sequence corresponded to the joined first and last names of the current United States Secretary of State, Colin Powell. Peptide COLINPOWELL (i.e., Cys-Orn-Leu-Ile-Asn-Pro-Orn-Trp-Glu-Leu-Leu) was synthesized as a C-terminal amide, and found to be inactive against bacteria [methicillin-resistant Staphylococcus aureus (M...<br/><br/>David Wade, De Yang, Michael A. Lea (2004) <i>Wade Research Foundation Reports</i> 1: 2-35 <br/>

http://publicationslist.org/david.wade/refid62 2009-12-10T09:34:34Z

D. Wade (2004) <i>Peptide Revolution: Genomics, Proteomics & Therapeutics</i> 580-581<br/>

http://publicationslist.org/david.wade/refid50 2009-12-09T10:48:12Z

David Wade (2003) <i>Chemistry Preprint Archive</i> 1: 159-170<br/>

http://publicationslist.org/david.wade/refid3 2009-12-09T05:10:47Z

Temporin A and structurally related peptides produced in amphibian dermal granular glands and in wasp venom were tested for growth inhibition of Batrachochytrium dendrobatidis, a pathogen associated with global amphibian declines. Two natural amphibian temporins, a wasp temporin, and six synthetic analogs effectively inhibited growth. Differences in potency due to amino acid substitution suggest t...<br/><br/>Louise A Rollins-Smith, Cynthia Carey, J Michael Conlon, Laura K Reinert, Jennifer K Doersam, Tomas Bergman, Jerzy Silberring, Hilkka Lankinen, David Wade (2003) <i>Antimicrob Agents Chemother</i> 47: 3 1157-1160<br/>

http://publicationslist.org/david.wade/refid23 2009-12-09T07:13:37Z

Several naturally occurring antimicrobial peptides, from mammals and insects, have previously been shown to be chemotactic for human inflammatory cells. Based on this evidence, ten synthetic analogs of naturally occurring antibiotic peptides from the skin secretions of three species of Ranid frogs and the venom of one species of Vespid wasp (i.e., T/V-like peptides) were tested for their abilities...<br/><br/>De Yang, Qian Chen, Joost J. Oppenheim, Pentti Kuusela, John W. Taylor and David Wade (2003) <i>Letters in Peptide Science</i> 10: 2 99-110<br/>

http://publicationslist.org/david.wade/refid24 2009-12-09T07:28:48Z

A group of 34 peptides, that have been isolated from the skins of frogs and the venom of wasps, are structurally similar to 10 antibiotic peptides, called temporins, isolated from the skin of the European red frog, Rana temporaria. These temporin-like peptides have antibiotic and neutrophil stimulating properties. A preliminary consensus sequence derived from 30 frog skin peptides was a good predi...<br/><br/>David Wade (2002) <i>Internet Journal of Chemistry</i> 5: 5 Article 5<br/>

http://publicationslist.org/david.wade/refid49 2009-12-09T10:43:14Z

A group of 34 peptides, that have been isolated from the skins of frogs and the venom of wasps, are structurally similar to 10 antibiotic peptides, called temporins, isolated from the skin of the European red frog, Rana temporaria. These temporin-like peptides have antibiotic and neutrophil stimulating properties. A preliminary consensus sequence derived from 30 frog skin peptides was a good pred...<br/><br/>David Wade (2002) <i>Chemistry Preprint Archive</i> 4: 280-300<br/>

http://publicationslist.org/david.wade/refid25 2009-12-09T07:37:22Z

A computerized database for synthetic antibiotic peptides, the SAPD, has been created and is available on the Internet at web address, http://oma.terkko.helsinki.fi:8080/~SAPD. The SAPD is modelled on two pre-existing computer databases for naturally occurring peptide antibiotics, the Antimicrobial Sequences Database and the Peptaibol Database, and it will contain both chemical and biological info...<br/><br/>David Wade, Jukka Englund (2002) <i>Protein Pept Lett</i> 9: 1 53-57<br/>

http://publicationslist.org/david.wade/refid12 2009-12-09T05:16:18Z

Temporin A, 18 analogs, and a cecropin A-temporin A hybrid peptide were tested with antibiotic sensitive and resistant bacteria, fungi, human erythrocytes, and in clotting assays.Several peptides were active in these assays, and some analogs (D-TA, W1-TA, and Con-L4,G10) may be useful lead compounds for further antibiotics development.The activity of temporin A was found to be dependent upon sever...<br/><br/>David Wade, Jan-Ingmar Flock, Charlotta Edlund, Ingegerd Löfving-Arvholm, Matti Sällberg, Tomas Bergman, Angela Silveira, Cecille Unson, Louise Rollins-Smith, Jerzy Silberring, Malcom Richardson, Pentti Kuusela, Hilkka Lankinen (2002) <i>Protein & Peptide Letters</i> 9: 6 533-543<br/>

http://publicationslist.org/david.wade/refid27 2009-12-09T07:44:51Z

Cysteine proteinase found in the spinal cord of rat, called nociceptin-converting enzyme (NCE), is competitively inhibited by dynorphin A and its fragment des-[Tyr(1)]-DYN A. This proteinase converts orphanin FQ/nociceptin (OFQ/N) to two major fragments: OFQ/N(1-11) and further OFQ/N(1-6) with analgesic properties. Dynorphin A at the concentration of 10 microM increases K(M) from 15.0 to 55.9 micr...<br/><br/>P Suder, D Wade, A Łegowska, J Kotlińska, K Rolka, J Silberring (2001) <i>Biochem Biophys Res Commun</i> 287: 4 927-931<br/>

http://publicationslist.org/david.wade/refid26 2009-12-09T07:42:05Z

Temporin A is one of a group of 31 structurally similar antibiotic peptides isolated from the skins of anurans, 10 of which were isolated from the European red frog, Rana temporaria. The synthesis and bacteriological study of temporin A and 8 analogs were reported previously, and this article describes the synthesis and analyses of 11 new temporin A analogs, and a cecropin A-temporin A hybrid pept...<br/><br/>David Wade, Tomas Bergman, Jerzy Silberring, and Hilkka Lankinen (2001) <i>Protein and Peptide Letters</i> 8: 6 443-450<br/>

http://publicationslist.org/david.wade/refid48 2009-12-09T10:38:07Z

CAMELs are a group of novel synthetic antibiotic peptides that may be effective substitutes for ciprofloxacin in the treatment of anthrax infections.<br/><br/>D. Wade (2001) <i>Chemistry Preprint Archive</i> 11: 86-88<br/>

http://publicationslist.org/david.wade/refid28 2009-12-09T08:08:30Z

David Wade (2001) <i>Challenges of Emerging Infections: New Pathogens or New Diseases</i> 119-124<br/>

http://publicationslist.org/david.wade/refid4 2009-12-09T05:18:28Z

Temporin A (TA) and a cecropin A-temporin A hybrid peptide (CATA) were synthesized and assayed for their hemolytic, anticoagulant, and antifungal properties. CATA retains significant antifungal activity, is less hemolytic than TA, and inhibits blood coagulation. These results recommend further studies of the biological activities of CATA.<br/><br/>D Wade, A Silveira, L Rollins-Smith, T Bergman, J Silberring, H Lankinen (2001) <i>Acta Biochim Pol</i> 48: 4 1185-1189<br/>

http://publicationslist.org/david.wade/refid5 2009-12-09T05:23:43Z

The increasing problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. One line of investigation is the synthesis of antimicrobial hybrid peptides. The aim of the present investigation was to determine the in vitro activities of 16 cecropin-melittin hybrid peptides (CAMEL analogues) against 60 anaerobic bacterial strains, to compare their activi...<br/><br/>H Oh, M Hedberg, D Wade, C Edlund (2000) <i>Antimicrob Agents Chemother</i> 44: 1 68-72<br/>

http://publicationslist.org/david.wade/refid11 2009-12-09T04:29:38Z

The temporins are a group of small, linear, basic, highly hydrophobic, antibiotic, peptide amides that were originally isolated from the skin of the European red frog, Rana temporaria. During the past decade, 30 temporin or temporin-like peptides have been isolated from the skins of Anurans. This article presents a brief review of the temporin literature, an analysis of the amino acid sequences of...<br/><br/>D. Wade, A. Silveira, J. Silberring, P. Kuusela, H. Lankinen (2000) <i>Protein and Peptide Letters</i> 7: 6 349-357<br/>

http://publicationslist.org/david.wade/refid29 2009-12-09T08:13:54Z

Temporin A (TA) is a small, basic, highly hydrophobic, antimicrobial peptide amide (FLPLIGRVLSGIL-NH2) found in the skin of the European red frog, Rana temporaria. It has variable antibiotic activities against a broad spectrum of microorganisms, including clinically important methicillin-sensitive and -resistant Staphylococcus aureus as well as vancomycin-resistant Enterococcus faecium strains. In...<br/><br/>D Wade, J Silberring, R Soliymani, S Heikkinen, I Kilpeläinen, H Lankinen, P Kuusela (2000) <i>FEBS Lett</i> 479: 1-2 6-9<br/>

http://publicationslist.org/david.wade/refid30 2009-12-09T08:40:15Z

Temporin A is a small, basic, highly hydrophobic, antibacterial peptide found in the skin of the European red frog, Rana temporaria. It was synthesized twice by the FastMoc solid phase method using amino acids protected at the N(alpha)-position with either 9-fluorenylmethoxycarbonyl or 2-(4-nitrophenylsulfonyl)ethoxycarbonyl. The syntheses of temporin A demonstrates the difference between 2-(4-nit...<br/><br/>I Harjunpää, P Kuusela, M T Smoluch, J Silberring, H Lankinen, D Wade (1999) <i>FEBS Lett</i> 449: 2-3 187-190<br/>

http://publicationslist.org/david.wade/refid31 2009-12-09T08:46:46Z

The topic of deuterium isotope effects is usually concerned with the effects on chemical reactions that are caused by the substitution of deuterium atoms for protium, or hydrogen, atoms in a molecule. These effects include changes in the rate of cleavage of covalent bonds to deuterium, or to an atom located adjacent to deuterium, in a reactant molecule. Deuterium isotope effects on other, noncoval...<br/><br/>D Wade (1999) <i>Chem Biol Interact</i> 117: 3 191-217<br/>

http://publicationslist.org/david.wade/refid59 2009-12-10T09:26:18Z

D. Wade (1999) <i>Innovation & Perspectives in Solid Phase Synthesis & Combinatorial Libraries 1998</i> 181-184<br/>

http://publicationslist.org/david.wade/refid61 2009-12-10T09:32:03Z

D. Wade, M. Sällberg, J. Silberring, J.-I. Flock (1999) <i>Innovation & Perspectives in Solid Phase Synthesis & Combinatorial Libraries 1998</i> 413-414<br/>

http://publicationslist.org/david.wade/refid60 2009-12-10T09:29:50Z

D. Wade, C. Edlund, Å Engström, M. Hedberg, J.-I. Flock (1999) <i>Innovation & Perspectives in Solid Phase Synthesis & Combinatorial Libraries 1998</i> 411-412<br/>

http://publicationslist.org/david.wade/refid6 2009-12-09T05:27:28Z

Efb (previously Fib) is a fibrinogen-binding protein secreted by Staphylococcus aureus. It has previously been shown that it plays a role in a wound infection model in the rat and that antibodies against Efb reduce the number of recovered bacteria from the mammary glands in a mouse mastitis model. Efb binds to the alpha-chain of fibrinogen and does not participate in bacterial adherence to fibrino...<br/><br/>M Palma, D Wade, M Flock, J I Flock (1998) <i>J Biol Chem</i> 273: 21 13177-13181<br/>

http://publicationslist.org/david.wade/refid34 2009-12-09T09:05:43Z

Staphylococcus aureus produces and secretes a protein, Efb, that binds to fibrinogen, seems to be required for virulence, and may benefit the microorganism by delaying wound healing. Interactions of Efb with fibrinogen are influenced by divalent metal cations, including Ca2+. Increasing concentrations of Ca2+ increased the binding of fibrinogen to immobilized Efb, whereas binding of Efb to immobil...<br/><br/>D Wade, M Palma, I Löfving-Arvholm, M Sällberg, J Silberring, J I Flock (1998) <i>Biochem Biophys Res Commun</i> 248: 3 690-695<br/>

http://publicationslist.org/david.wade/refid57 2009-12-10T09:22:29Z

D. Wade, R.B. Merrifield (1998) <i>Peptides 1996</i> 887-888<br/>

http://publicationslist.org/david.wade/refid32 2009-12-09T08:55:21Z

OBJECTIVE: Several small, 15-residue peptides that contain portions of the amino acid sequences of both cecropin A and melittin have previously been shown to have broad-spectrum antibacterial activities against aerobic microorganisms, with no undesirable hemolytic properties. It would also be useful to know what effect these hybrid peptides have on anaerobic bacteria. METHODS: The minimum inhibito...<br/><br/> C.Edlund, M. Hedberg, Å. Engström, J.-I. Flock, D. Wade (1998) <i>Clin Microbiol Infect</i> 4: 4 181-185<br/>

http://publicationslist.org/david.wade/refid58 2009-12-10T09:22:00Z

D. Wade (1998) <i>Peptides 1996</i> 889-890<br/>

http://publicationslist.org/david.wade/refid33 2009-12-09T09:01:17Z

Staphylococcus aureus produces and secretes a small, basic protein, designated Efb, that binds to fibrinogen and seems to be required for virulence of the organism. A 3D model of Efb was developed, and it predicts that the C-terminal half of the protein contains substantial a-helical content. CD analysis of Efb yielded a value of 40-41% a-helix. Calcium and zinc both influence the interactions bet...<br/><br/>David Wade, Marco Palma, Jan-Ingmar Flock, Kurt D. Berndt, Jerzy Silberring and Stan G. Galaktionov (1998) <i>Protein and Peptide Letters</i> 5: 4 199-206<br/>

http://publicationslist.org/david.wade/refid56 2009-12-10T09:23:01Z

D. Wade, P. Semchuk, R.S. Hodges (1996) <i>Peptides: Chemistry, Structure and Biology</i> 796-797<br/>

http://publicationslist.org/david.wade/refid45 2009-12-09T10:19:26Z

Antibiotically and/or antimalarially active D-peptides of naturally occurring antibiotics such as cecropin A, B and D, melittin, and Magainin I and II and their addition, deletion and replacement analogs including homologous and heterologous analogs thereof.<br/><br/>R.B. Merrifield, D. Wade, H.G. Boman (1996) <i>United State Patent Office (US Patent 5585353)</i><br/>

http://publicationslist.org/david.wade/refid35 2009-12-09T09:12:22Z

D. Wade (1996) <i>Protein and Peptide Letters</i> 3: 3 169-176<br/>

http://publicationslist.org/david.wade/refid55 2009-12-10T09:12:30Z

D. Wade, H.G. Boman, S.A. Mitchell, R.B. Merrifield (1994) <i>Peptides: Chemistry, Structure and Biology</i> 470-472<br/>

http://publicationslist.org/david.wade/refid38 2009-12-09T09:20:51Z

Melittin, a 26-residue bee venom peptide, is known to induce murine Abs specific for its hydrophilic C-terminus of residues 20-26 and T cell responses specific for its hydrophobic mid-region of residue 11-19. Synthetic melittin analogues with transposed sequences of Ac(21-26) (1-20) and Ac(26-21) (1-20) are found to induce murine Abs specific for the transposed peptide segment and to induce T cell...<br/><br/>T P King, D Wade, M R Coscia, S Mitchell, L Kochoumian, B Merrifield (1994) <i>J Immunol</i> 153: 3 1124-1131<br/>

http://publicationslist.org/david.wade/refid36 2009-12-09T09:15:36Z

The last decade has seen the rapid development of research investigating the mechanisms of apoptosis in a variety of experimental systems. Among the multitude of changes observed in apoptotic cells, chromatin cleavage is considered a biochemical hallmark of apoptosis. Chromatin fragmentation is an enzymatic process which depends on the activity of endogenous nuclease(s) and the susceptibility of c...<br/><br/>B Zhivotovsky, D Wade, P Nicotera, S Orrenius (1994) <i>Int Arch Allergy Immunol</i> 105: 4 333-338<br/>

http://publicationslist.org/david.wade/refid37 2009-12-09T09:18:27Z

Isolated rat liver nuclei were incubated in the presence of divalent cations, and the mechanisms underlying the subsequent chromatin fragmentation were investigated. Either of the two cations, Ca2+ or Mg2+ was sufficient to produce chromatin fragments with sizes between 700 and 300 kbp. The formation of chromatin fragments of 50 kbp as well as the following internucleosomal DNA cleavage--which are...<br/><br/>B Zhivotovsky, D Wade, A Gahm, S Orrenius, P Nicotera (1994) <i>FEBS Lett</i> 351: 2 150-154<br/>

http://publicationslist.org/david.wade/refid54 2009-12-10T09:08:58Z

R.B. Merrifield, H.G. Boman, D. Andreu, E.L. Merrifield, S. Mitchell, D. Wade (1993) <i>Peptide Chemistry 1992-Proceedings of the 2nd Japan Symposium on Peptide Chemistry,</i> 289-292<br/>

http://publicationslist.org/david.wade/refid40 2009-12-09T09:30:25Z

We have earlier reported two 26-residue antibacterial peptides made up from different segments of cecropin A (CA) and melittin (M). We now report a substantial reduction in size at the C-terminal section of the highly active hybrid CA(1-8)M(1-18), leading to a series of 20-, 18- and 15-residue analogs with antibiotic properties similar to the larger molecule. In particular, the 15-residue hybrids ...<br/><br/>D Andreu, J Ubach, A Boman, B Wåhlin, D Wade, R B Merrifield, H G Boman (1992) <i>FEBS Lett</i> 296: 2 190-194<br/>

http://publicationslist.org/david.wade/refid53 2009-12-10T09:04:36Z

D. Wade, S.A. Mitchell, H.G. Boman, A. Boman, R.B. Merrifield (1992) <i>Peptides: Chemistry and Biology</i> 435-436<br/>

http://publicationslist.org/david.wade/refid39 2009-12-09T09:27:28Z

Eight new analogs of cecropin A, two new analogs of melittin and 30 hybrid peptides containing sequences from cecropins and melittin have been synthesized. The lengths of the peptides have varied from 37 residues (the length of cecropin A) to 18 residues. The peptides have been assayed for lysis of sheep red blood cells and for antibacterial activity against two Gram negative and three Gram positi...<br/><br/>D Wade, D Andreu, S A Mitchell, A M Silveira, A Boman, H G Boman, R B Merrifield (1992) <i>Int J Pept Protein Res</i> 40: 5 429-436<br/>

http://publicationslist.org/david.wade/refid52 2009-12-10T09:00:22Z

R.B. Merrifield, H.G. Boman, D. Andreu, Z.-Q. Li, J. Fink, D. Wade (1991) <i>Peptides 1990</i> 3-16<br/>

http://publicationslist.org/david.wade/refid42 2009-12-09T09:49:25Z

The cecropins are a family of small, basic peptides which have been isolated from insects and mammals, and shown to possess broad-spectrum antibacterial activities. This article reviews recent research into the structures and functions of cecropins, and concentrates on work involving the solid-phase synthesis and study of cecropin analogs, model peptides, and hybrids of cecropin and melittin, the ...<br/><br/>D. Wade, R.B. Merrifield, and H.G. Boman (1991) <i>Surface Reactive Peptides and Polymers: Discovery and Commercialization</i> 237-248<br/>

http://publicationslist.org/david.wade/refid41 2009-12-09T09:36:03Z

Deuteration of N-nitrosodimethylamine (NDMA) decreases its carcinogenicity and produces an isotope effect on its metabolism. Our previous results showed that deuteration causes a 5-fold increase in the apparent Km, but not the Vmax, for the demethylation and denitrosation of NDMA in microsomes. In the present work, we studied the nature of this deuterium isotope effect with several compounds using...<br/><br/>C S Yang, H Ishizaki, M J Lee, D Wade, A Fadel (1991) <i>Chem Res Toxicol</i> 4: 4 408-413<br/>

http://publicationslist.org/david.wade/refid7 2009-12-09T05:30:48Z

The D enantiomers of three naturally occurring antibiotics--cecropin A, magainin 2 amide, and melittin--were synthesized. In addition, the D enantiomers of two synthetic chimeric cecropin-melittin hybrid peptides were prepared. Each D isomer was shown by circular dichroism to be a mirror image of the corresponding L isomer in several solvent mixtures. In 20% hexafluoro-2-propanol the peptides cont...<br/><br/>D Wade, A Boman, B Wåhlin, C M Drain, D Andreu, H G Boman, R B Merrifield (1990) <i>Proc Natl Acad Sci U S A</i> 87: 12 4761-4765<br/>

http://publicationslist.org/david.wade/refid51 2009-12-10T08:56:04Z

D. Wade, R.B. Merrifield, and H.G. Boman (1990) <i>Peptides: Chemistry, Structure and Biology</i> 120-121<br/>

http://publicationslist.org/david.wade/refid43 2009-12-09T09:54:26Z

Solid phase synthesis was used to produce 5 hybrid peptides containing sequences from the antibacterial peptide, cecropin A, and from the bee venom toxin, melittin. Four of these chimeric peptides showed good antibacterial activity against representative Gram-negative and Gram-positive bacterial species. The best hybrid, cecropin A(1-13)-melittin(1-13) was 100-fold more active than cecropin A agai...<br/><br/>H G Boman, D Wade, I A Boman, B Wåhlin, R B Merrifield (1989) <i>FEBS Lett</i> 259: 1 103-106<br/>

http://publicationslist.org/david.wade/refid46 2009-12-09T10:26:04Z

David Wade (1988) <br/>

http://publicationslist.org/david.wade/refid44 2009-12-09T10:12:38Z

C.S. Yang, D. Wade, T. Anjo, L.K. Keefer (1987) <i>Relevance of N-nitroso compounds to human cancer: exposure and mechanisms.</i> 124-128<br/>

http://publicationslist.org/david.wade/refid8 2009-12-09T05:33:12Z

The present study was undertaken to examine the nature of the low Km (KmI) form of rat liver microsomal N-nitrosodimethylamine demethylase (NDMAd) and its inhibition by organic compounds which are commonly present in the assay mixture. Using radiometric and colorimetric assay methods with an NADPH-generating system consisting of 0.4 mM NADP, 10 mM glucose-6-phosphate, and glucose-6-phosphate dehyd...<br/><br/>J S Yoo, R J Cheung, C J Patten, D Wade, C S Yang (1987) <i>Cancer Res</i> 47: 13 3378-3383<br/>

http://publicationslist.org/david.wade/refid10 2009-12-09T05:37:01Z

With the goal of identifying the organic amine product(s) of enzymatic N-nitrosodimethylamine (NDMA) denitrosation, 4 mM NDMA was incubated with liver microsomes from ethanol-treated rats. The concentrations of dimethylamine and methylamine were determined by derivatization with 2,4-dinitrofluorobenzene followed by gas chromatography-mass spectrometry. There was no net increase in the concentratio...<br/><br/>L K Keefer, T Anjo, D Wade, T Wang, C S Yang (1987) <i>Cancer Res</i> 47: 2 447-452<br/>

http://publicationslist.org/david.wade/refid9 2009-12-09T05:35:32Z

In an attempt to elucidate the molecular basis for the decrease in rat liver carcinogenicity and DNA-alkylating ability that accompanies deuteration of N-nitrosodimethylamine (NDMA), NDMA and its fully deuterated analogue ([2H6]NDMA) were incubated with acetone-induced rat liver microsomes. Rates for the competing metabolic routes, denitrosation and demethylation, were determined from colorimetric...<br/><br/>D Wade, C S Yang, C J Metral, J M Roman, J A Hrabie, C W Riggs, T Anjo, L K Keefer, B A Mico (1987) <i>Cancer Res</i> 47: 13 3373-3377<br/>

http://publicationslist.org/david.wade/refid47 2009-12-09T10:28:08Z

David Wade (1982) <br/>