http://publicationslist.org/data/derrick.robinson/atom.xml Derrick Robinsonhttp://publicationslist.org/derrick.robinson$basepathfavicon.icoRecent additions to Derrick Robinson's PublicationsList.org pagehttp://publicationslist.org/publications.png2009-09-29T15:41:22Z http://publicationslist.org/derrick.robinson/refid24 2009-09-29T15:38:41Z

In trypanosomes, the flagellum is rooted in the flagellar pocket, a surface micro-domain that is the sole site for endocytosis and exocytosis. By analysis of anterograde or retrograde intraflagellar transport in IFT88RNAi or IFT140RNAi mutant cells, we show that elongation of the new flagellum is not required for flagellar pocket formation but is essential for its organisation, orientation and fun...<br/><br/>Sabrina Absalon, Thierry Blisnick, Mélanie Bonhivers, Linda Kohl, Nadège Cayet, Géraldine Toutirais, Johanna Buisson, Derrick Robinson, Philippe Bastin (2008) <i>J Cell Sci</i> <i></i> <i></i> 121: Pt 22 3704-3716<br/>

http://publicationslist.org/derrick.robinson/refid25 2009-09-29T15:39:39Z

ABSTRACT: BACKGROUND: Trypanosoma brucei is a haemoflagellate pathogen of man, wild animals and domesticated livestock in central and southern Africa. In all life cycle stages this parasite has a single mitochondrion that contains a uniquely organised genome that is condensed into a flat disk-like structure called the kinetoplast. The kinetoplast is essential for insect form procyclic cells and th...<br/><br/>Mélanie Bonhivers, Nicolas Landrein, Marion Decossas, Derrick R Robinson (2008) <i>Parasit Vectors</i> <i></i> <i></i> 1: 1 <br/>

http://publicationslist.org/derrick.robinson/refid26 2009-09-29T15:39:39Z

Trypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket (FP), an invagination of the pellicular membrane. The pocket is the sole site for specific receptors...<br/><br/>Mélanie Bonhivers, Sophie Nowacki, Nicolas Landrein, Derrick R Robinson (2008) <i>PLoS Biol</i> <i></i> <i></i> 6: 5 <br/>

http://publicationslist.org/derrick.robinson/refid27 2009-09-29T15:38:41Z

We present here the characterisation of the Leishmania small G protein ADP-Ribosylation Factor-Like protein 1 (ARL-1). The ARL-1 gene is present in one copy per haploid genome and conserved among trypanosomatids. It encodes a protein of 20 kDa, which is equally expressed in the insect promastigote and mammalian amastigote forms of the parasite. ARL-1 localises to the Trans-Golgi Network (TGN); N-t...<br/><br/>Annelise Sahin, Benoît Espiau, Emmanuel Tetaud, Armelle Cuvillier, Lydia Lartigue, Audrey Ambit, Derrick R Robinson, Gilles Merlin (2008) <i>PLoS One</i> <i></i> <i></i> 3: 2 <br/>

http://publicationslist.org/derrick.robinson/refid28 2009-09-29T15:38:41Z

Kinetoplast DNA (kDNA), the trypanosome mitochondrial genome, is a giant network containing several thousand interlocked DNA rings. Within the mitochondrion, kDNA is condensed into a disk-shaped structure positioned near the flagellar basal body. The disk is linked to the basal body by a remarkable transmembrane filament system named the tripartite attachment complex (TAC). Following kDNA replicat...<br/><br/>Zhixing Zhao, Megan E Lindsay, Arnab Roy Chowdhury, Derrick R Robinson, Paul T Englund (2008) <i>EMBO J</i> <i></i> <i></i> 27: 1 143-154<br/>

http://publicationslist.org/derrick.robinson/refid19 2007-11-22T13:41:15Z

Kinetoplast DNA (kDNA), the trypanosome mitochondrial genome, is a giant network containing several thousand interlocked DNA rings. Within the mitochondrion, kDNA is condensed into a disk-shaped structure positioned near the flagellar basal body. The disk is linked to the basal body by a remarkable transmembrane filament system named the tripartite attachment complex (TAC). Following kDNA repli...<br/><br/>Zhixing Zhao, Megan, E. Lindsay, Arnab Roy Chowdhury, Derrick Robinson, and Paul T. Englund. (2007) <i>EMBO. J</i> in press: <br/>

http://publicationslist.org/derrick.robinson/refid29 2009-09-29T15:38:41Z

Kinetoplast DNA (kDNA) is the remarkable mitochondrial genome of trypanosomatids. Its major components are several thousands of topologically linked DNA minicircles, whose replication origins are bound by the universal minicircle sequence-binding protein (UMSBP). The cellular function of UMSBP has been studied in Trypanosoma brucei by using RNAi analysis. Silencing of the trypanosomal UMSBP genes ...<br/><br/>Neta Milman, Shawn A Motyka, Paul T Englund, Derrick Robinson, Joseph Shlomai (2007) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 104: 49 19250-19255<br/>

http://publicationslist.org/derrick.robinson/refid20 2007-11-22T13:43:31Z

Kinetoplast DNA (kDNA) is the remarkable mitochondrial genome of trypanosomatids. Its major components are several thousands of topologically-linked DNA minicircles, whose replication origins are bound by the universal minicircle sequence binding protein (UMSBP). The cellular function of UMSBP has been studied in Trypanosoma brucei using RNA interference (RNAi) analysis. Silencing of the trypanoso...<br/><br/>Neta Milman, Shawn A. Motyka, Paul T. Englund, Derrick Robinson, and Joseph Shlomai (2007) <i>PNAS</i> in press: <br/>

http://publicationslist.org/derrick.robinson/refid15 2009-09-29T15:38:41Z

To perform their multiple functions, cilia and flagella are precisely positioned at the cell surface by mechanisms that remain poorly understood. The protist Trypanosoma brucei possesses a single flagellum that adheres to the cell body where a specific cytoskeletal structure is localised, the flagellum attachment zone (FAZ). Trypanosomes build a new flagellum whose distal tip is connected to the s...<br/><br/>Sabrina Absalon, Linda Kohl, Carole Branche, Thierry Blisnick, Géraldine Toutirais, Filippo Rusconi, Jacky Cosson, Mélanie Bonhivers, Derrick Robinson, Philippe Bastin (2007) <i>PLoS One</i> <i></i> <i></i> 2: 5 <br/>

http://publicationslist.org/derrick.robinson/refid18 2009-09-29T15:38:41Z

Matrix metalloproteinase (MMP)-3 is a protease involved in cancer progression and tissue remodeling. Using immunofluorescence and immunoelectron microscopy, we identified nuclear localization of MMP-3 in several cultured cell types and in human liver tissue sections. Western blot analysis of nuclear extracts revealed two immunoreactive forms of MMP-3 at 35 and 45 kd, with the 35-kd form exhibiting...<br/><br/>Karim Si-Tayeb, Arnaud Monvoisin, Claire Mazzocco, Sébastien Lepreux, Marion Decossas, Gaëlle Cubel, Danièle Taras, Jean-Frédéric Blanc, Derrick R Robinson, Jean Rosenbaum (2006) <i>Am J Pathol</i> <i></i> <i></i> 169: 4 1390-1401<br/>

http://publicationslist.org/derrick.robinson/refid1 2009-09-29T15:38:41Z

The NIMA-related kinase 2 (NEK 2) has important cell cycle functions related to centriole integrity and splitting. Trypanosoma brucei does not possess centrioles, however, cytokinesis is coupled to basal body separation events. Here we report the first functional characterisation of a T. brucei basal body-cytoskeletal NIMA-related kinase (NRK) protein, TbNRKC. The TbNRKC kinase domain has high ami...<br/><br/>Lydie C Pradel, Mélanie Bonhivers, Nicolas Landrein, Derrick R Robinson (2006) <i>J Cell Sci</i> <i></i> <i></i> 119: Pt 9 1852-1863<br/>

http://publicationslist.org/derrick.robinson/refid13 2007-11-22T13:33:59Z

Flagella and cilia are elaborate cytoskeletal structures conserved from protists to mammals, where they fulfil functions related to motility or sensitivity. Here we demonstrate novel roles for the flagellum in the control of cell size, shape, polarity and division of the protozoan Trypanosoma brucei. To investigate the function of the flagellum, its formation was perturbed by inducible RNA interfe...<br/><br/>Linda Kohl, Derrick Robinson, Philippe Bastin (2003) <i>EMBO J</i> <i></i> <i></i> 22: 20 5336-5346<br/>

http://publicationslist.org/derrick.robinson/refid14 2009-09-29T15:38:41Z

Flagella and cilia are elaborate cytoskeletal structures conserved from protists to mammals, where they fulfil functions related to motility or sensitivity. We demonstrate a novel role for the flagellum in the control of cell morphogenesis and division of Trypanosoma brucei. To investigate flagellum functions, its formation was perturbed by inducible RNA interference silencing of components requir...<br/><br/>Linda Kohl, Derrick Robinson, Philippe Bastin (2003) <i>J Soc Biol</i> <i></i> <i></i> 197: 4 379-387<br/>

http://publicationslist.org/derrick.robinson/refid2 2007-11-22T13:28:43Z

In trypanosomes, the large mitochondrial genome within the kinetoplast is physically connected to the flagellar basal bodies and is segregated by them during cell growth. The structural linkage enabling these phenomena is unknown. We have developed novel extraction/fixation protocols to characterize the links involved in kinetoplast-flagellum attachment and segregation. We show that three specific...<br/><br/>Emmanuel O Ogbadoyi, Derrick R Robinson, Keith Gull (2003) <i>Mol Biol Cell</i> <i></i> <i></i> 14: 5 1769-1779<br/>

http://publicationslist.org/derrick.robinson/refid3 2007-11-22T13:28:43Z

The subpellicular microtubules of the trypanosome cytoskeleton are cross-linked to each other and the plasma membrane, creating a cage-like structure. We have isolated, from Trypanosoma brucei, two related low-molecular-weight cytoskeleton-associated proteins (15- and 17-kDa), called CAP15 and CAP17, which are differentially expressed during the life cycle. Immunolabeling shows a corset-like coloc...<br/><br/>Cécile Vedrenne, Christiane Giroud, Derrick R Robinson, Sébastien Besteiro, Christophe Bosc, Frédéric Bringaud, Théo Baltz (2002) <i>Mol Biol Cell</i> <i></i> <i></i> 13: 3 1058-1070<br/>

http://publicationslist.org/derrick.robinson/refid5 2007-11-22T13:28:43Z

Kinetoplast DNA (kDNA), the mitochondrial DNA of the trypanosomatid Crithidia fasciculata, is a unique structure containing 5,000 DNA minicircles topologically linked into a massive network. In vivo, the network is condensed into a disk-shaped structure. Replication of minicircles initiates at unique origins that are bound by universal minicircle sequence (UMS)-binding protein (UMSBP), a sequence-...<br/><br/>K Abu-Elneel, D R Robinson, M E Drew, P T Englund, J Shlomai (2001) <i>J Cell Biol</i> <i></i> <i></i> 153: 4 725-734<br/>

http://publicationslist.org/derrick.robinson/refid4 2007-11-22T13:28:43Z

Cell differentiation in Trypanosoma brucei involves highly regulated changes in morphology, proliferation and metabolism. However, the controls of these developmental processes are unknown. We have identified two novel proteins from the rare CCCH zinc finger family, each <140 amino acids in length and implicated in life cycle regulation. TbZFP1 is transiently enriched during differentiation from t...<br/><br/>E F Hendriks, D R Robinson, M Hinkins, K R Matthews (2001) <i>EMBO J</i> <i></i> <i></i> 20: 23 6700-6711<br/>

http://publicationslist.org/derrick.robinson/refid11 2007-11-22T13:29:43Z

Import of the ADP/ATP carrier (AAC) into mitochondria requires the soluble TIM10 complex to cross the intermembrane space. We report here that Tim9 and Tim10 purified from Escherichia coli can form a complex of the same size as the endogenous complex from yeast mitochondria. This shows that no other mitochondrial protein is required for the formation of the TIM10 complex. Co-expression of both pro...<br/><br/>P Luciano, S Vial, M A Vergnolle, S D Dyall, D R Robinson, K Tokatlidis (2001) <i>EMBO J</i> 20: 15 4099-4106<br/>

http://publicationslist.org/derrick.robinson/refid17 2007-11-22T13:35:20Z

The structural basis of mitosis, spindle organisation and chromosome segregation, in the unicellular parasite Trypanosoma brucei is poorly understood. Here, using immunocytochemistry, fluorescent in situ hybridisation and electron microscopy, we provide a detailed analysis of mitosis in this parasite. We describe the organisation of the mitotic spindle during different stages of mitosis, the compl...<br/><br/>E Ogbadoyi, K Ersfeld, D Robinson, T Sherwin, K Gull (2000) <i>Chromosoma</i> <i></i> <i></i> 108: 8 501-513<br/>

http://publicationslist.org/derrick.robinson/refid6 2007-11-22T13:28:43Z

In Trypanosoma brucei, we have cloned a gene approximately 5 kb downstream of the glucose transporter gene cluster, containing a variable number of 102 bp repeats. This gene encodes a protein with no homologues in the data bases. Antibodies raised against the 34 amino acids repeated motif recognized proteins ranging from 145 to 270 kDa, depending on strains, in both bloodstream and procyclic forms...<br/><br/>F Bringaud, D R Robinson, S Barradeau, N Biteau, D Baltz, T Baltz (2000) <i>Mol Biochem Parasitol</i> <i></i> <i></i> 111: 2 283-297<br/>

http://publicationslist.org/derrick.robinson/refid7 2007-11-22T13:28:43Z

Trypanosoma brucei has a single nucleus and a single kinetoplast (the mitochondrial genome). Each of these organelles has a distinct S phase, which is followed by a segregation period, prior to cell division. The segregation of the two genomes takes place in a specific temporal order by interaction with microtubule-based structures, the spindle for nuclear DNA and the flagellum basal bodies for th...<br/><br/>A Ploubidou, D R Robinson, R C Docherty, E O Ogbadoyi, K Gull (1999) <i>J Cell Sci</i> <i></i> <i></i> 112 ( Pt 24): 4641-4650<br/>

http://publicationslist.org/derrick.robinson/refid8 2007-11-22T13:28:43Z

Trypanosoma brucei has a precisely ordered microtubule cytoskeleton whose morphogenesis is central to cell cycle events such as organelle positioning, segregation, mitosis, and cytokinesis. We have defined microtubule polarity and show the + ends of the cortical microtubules to be at the posterior end of the cell. Measurements of organelle positions through the cell cycle reveal a high degree of c...<br/><br/>D R Robinson, T Sherwin, A Ploubidou, E H Byard, K Gull (1995) <i>J Cell Biol</i> <i></i> <i></i> 128: 6 1163-1172<br/>

http://publicationslist.org/derrick.robinson/refid23 2007-11-22T14:05:17Z

Robinson, D. R. Scott, V. Sherwin, T. Ploubidou, A. and K. Gull. (1995) <i>Protistological Actualities (Proceedings of the Second European Congress of Protistology). </i> 1.: 66–67. <br/>

http://publicationslist.org/derrick.robinson/refid9 2007-11-22T13:28:43Z

The kinetoplast is a concatenated network of circular DNA molecules found in the mitochondrion of many trypanosomes. This mass of DNA is replicated in a discrete "S" phase in the cell cycle. We have tracked the incorporation of the thymidine analogue 5-bromodeoxyuridine into newly replicated DNA by immunofluorescence and novel immunogold labeling procedures. This has allowed the detection of parti...<br/><br/>D R Robinson, K Gull (1994) <i>J Cell Biol</i> <i></i> <i></i> 126: 3 641-648<br/>

http://publicationslist.org/derrick.robinson/refid21 2007-11-22T13:46:31Z

Robinson, D. R. (1994) <i>Biological Sciences Review. </i> 3, 27–29.: <br/>

http://publicationslist.org/derrick.robinson/refid10 2007-11-22T13:28:43Z

The mitochondrial genome of Trypanosoma brucei is organized in the form of a complex catenated network of circular DNA molecules. This mass of DNA, known as the kinetoplast, is present at a unique site in the single mitochondrion, and is replicated in a discrete, periodic S phase of the cell cycle. The single-copy nature of the kinetoplast suggests that there is a mechanism ensuring segregation fi...<br/><br/>D R Robinson, K Gull (1991) <i>Nature</i> <i></i> <i></i> 352: 6337 731-733<br/>

http://publicationslist.org/derrick.robinson/refid12 2007-11-22T13:32:48Z

D Robinson, P Beattie, T Sherwin, K Gull (1991) <i>Methods Enzymol</i> 196: 285-299<br/>

http://publicationslist.org/derrick.robinson/refid22 2007-11-22T14:03:01Z

Gull, K, Birkett, C. Gerke–Bonet, R, Parma, A, Robinson, D, R, Sherwin, T, and R. Woodward, (1990). (1990) <i>Biochemical Society Transactions</i> 18,: 720–722. <br/>

http://publicationslist.org/derrick.robinson/refid16 2007-11-22T13:35:20Z

K Gull, C Birkett, R Gerke-Bonet, A Parma, D Robinson, T Sherwin, R Woodward (1990) <i>Biochem Soc Trans</i> <i></i> <i></i> 18: 5 720-722<br/>