http://publicationslist.org/data/e.palmer/atom.xml Ed Palmerhttp://publicationslist.org/e.palmer$basepathfavicon.icoRecent additions to Ed Palmer's PublicationsList.org pagehttp://publicationslist.org/publications.png2009-08-22T14:57:50Z http://publicationslist.org/e.palmer/refid87 2009-08-22T14:19:46Z

Following infection, naïve CD8+ T cells bearing pathogen-specific T cell receptors (TCRs) differentiate into a mixed population of short-lived effector and long-lived memory T cells to mediate an adaptive immune response. How the TCR regulates memory T cell development has remained elusive. Using a mutant TCR transgenic model, we found that point mutations in the TCR beta transmembrane domain (be...<br/><br/>Emma Teixeiro, Mark A Daniels, Sara E Hamilton, Adam G Schrum, Rafael Bragado, Stephen C Jameson, Ed Palmer (2009) <i>Science</i> 323: 5913 502-505<br/>

http://publicationslist.org/e.palmer/refid78 2009-08-22T14:16:15Z

The affinity of the T-cell receptor (TCR) for self antigen is the basis for the selection of a useful (MHC-restricted) and safe (self-tolerant) T-cell repertoire. However, it has been difficult to understand how thymocytes measure ligand affinity and translate this signal into a cellular response. In this Opinion article, we propose a new model that describes how the TCR discriminates between low-...<br/><br/>Ed Palmer, Dieter Naeher (2009) <i>Nat Rev Immunol</i> <i></i> <i></i> 9: 3 207-213<br/>

http://publicationslist.org/e.palmer/refid88 2009-08-22T14:20:58Z

The ex vivo induction of alloantigen-specific hyporesponsiveness by costimulatory pathway blockade or exposure to immunoregulatory cytokines has been shown to inhibit proliferation, IL-2 production, and the graft-versus-host disease (GVHD) capacity of adoptively transferred T-cells. We hypothesized that inhibition of the intracellular NF-kappaB pathway in alloreactive T-cells, which is critical fo...<br/><br/>M J O'Shaughnessy, C Vogtenhuber, K Sun, R Sitcheran, A S Baldwin, W J Murphy, L Dang, B Jaffee, E Palmer, J S Serody, B R Blazar (2009) <i>Am J Transplant</i> <i></i> <i></i> 9: 3 452-462<br/>

http://publicationslist.org/e.palmer/refid100 2009-08-22T14:31:55Z

The use of appropriate fluorescent proteins has allowed the use of FRET microscopy for investigation of intermolecular interactions in living cells. This method has the advantage of both being dynamic and of working at the subcellular level, so that the time and place where proteins interact can be visualized. We have used FRET microscopy to analyze the interactions between the T cell antigen rece...<br/><br/>Nicholas R J Gascoigne, Jeanette Ampudia, Jean-Pierre Clamme, Guo Fu, Carina Lotz, Michel Mallaun, Nathalie Niederberger, Ed Palmer, Vasily Rybakin, Pia P Yachi, Tomasz Zal (2009) <i>Curr Top Microbiol Immunol</i> 334: 31-46<br/>

http://publicationslist.org/e.palmer/refid90 2009-08-22T14:22:34Z

TCR engagement by peptide-MHC class I (pMHC) ligands induces a conformational change (Deltac) in CD3 (CD3Deltac) that contributes to T cell signaling. We found that when this interaction took place between primary T lineage cells and APCs, the CD8 coreceptor was required to generate CD3Deltac. Interestingly, neither enhancement of Ag binding strength nor Src kinase signaling explained this corecep...<br/><br/>Diana Gil, Adam G Schrum, Mark A Daniels, Ed Palmer (2008) <i>J Immunol</i> <i></i> <i></i> 180: 6 3900-3909<br/>

http://publicationslist.org/e.palmer/refid79 2009-08-22T14:16:15Z

The CD8 coreceptor contributes to the recognition of peptide-MHC (pMHC) ligands by stabilizing the TCR-pMHC interaction and enabling efficient signaling initiation. It is unclear though, which structural elements of the TCR ensure a productive association of the coreceptor. The alpha-chain connecting peptide motif (alpha-CPM) is a highly conserved sequence of eight amino acids in the membrane prox...<br/><br/>Michel Mallaun, Dieter Naeher, Mark A Daniels, Pia P Yachi, Barbara Hausmann, Immanuel F Luescher, Nicholas R J Gascoigne, Ed Palmer (2008) <i>J Immunol</i> <i></i> <i></i> 180: 12 8211-8221<br/>

http://publicationslist.org/e.palmer/refid89 2009-08-22T14:20:58Z

Cyclic AMP (cAMP) is an important negative regulator of T cell activation, and an increased level of cAMP is associated with T cell hyporesponsiveness in vitro. We sought to determine whether elevating intracellular cAMP levels ex vivo in alloreactive T cells during primary mixed lymphocyte reactions (MLR) is sufficient to induce alloantigen-specific tolerance and prevent graft-versus-host disease...<br/><br/>Matthew J O'Shaughnessy, Zong-Ming Chen, Irene Gramaglia, Patricia A Taylor, Angela Panoskaltsis-Mortari, Christine Vogtenhuber, Ed Palmer, Thomas Grader-Beck, Vassiliki A Boussiotis, Bruce R Blazar (2007) <i>Biol Blood Marrow Transplant</i> <i></i> <i></i> 13: 5 530-542<br/>

http://publicationslist.org/e.palmer/refid91 2009-08-22T14:22:34Z

Most mechanisms of cell development, physiology, and signal transduction are controlled by protein-protein interactions. Immunoprecipitation of multiprotein complexes detected by flow cytometry (IP-FCM) is a means to quantitatively measure these interactions. The high sensitivity of this method makes it useful even when very little biomaterial is available for analysis, as in the case of rare prim...<br/><br/>Adam G Schrum, Diana Gil, Elaine P Dopfer, David L Wiest, Laurence A Turka, Wolfgang W A Schamel, Ed Palmer (2007) <i>Sci STKE</i> <i></i> <i></i> 2007: 389 <br/>

http://publicationslist.org/e.palmer/refid80 2009-08-22T14:16:15Z

T cell tolerance depends on the T cell receptor's affinity for peptide/major histocompatibility complex (MHC) ligand; this critical parameter determines whether a thymocyte will be included (positive selection) or excluded (negative selection) from the T cell repertoire. A quantitative analysis of ligand binding was performed using an experimental system permitting receptor-coreceptor interactions...<br/><br/>Dieter Naeher, Mark A Daniels, Barbara Hausmann, Philippe Guillaume, Immanuel Luescher, Ed Palmer (2007) <i>J Exp Med</i> <i></i> <i></i> 204: 11 2553-2559<br/>

http://publicationslist.org/e.palmer/refid109 2009-08-22T14:49:28Z

Ed Palmer (2007) <i>Swiss Med Wkly</i> <i></i> <i></i> 137 Suppl 155: 99S-100S<br/>

http://publicationslist.org/e.palmer/refid110 2009-08-22T14:49:28Z

The immune system can be roughly divided into innate and adaptive compartments. The adaptive compartment includes the B and T lymphocytes, whose antigen receptors are generated by recombination of gene segments. The consequence is that the creation of self-reactive lymphocytes is unavoidable. For the host to remain viable, the immune system has evolved a strategy for removing autoimmune lymphocyte...<br/><br/>Ed Palmer (2006) <i>J Recept Signal Transduct Res</i> <i></i> <i></i> 26: 5-6 367-378<br/>

http://publicationslist.org/e.palmer/refid66 2009-08-22T14:08:30Z

A healthy individual can mount an immune response to exogenous pathogens while avoiding an autoimmune attack on normal tissues. The ability to distinguish between self and non-self is called 'immunological tolerance' and, for T lymphocytes, involves the generation of a diverse pool of functional T cells through positive selection and the removal of overtly self-reactive thymocytes by negative sele...<br/><br/>Mark A Daniels, Emma Teixeiro, Jason Gill, Barbara Hausmann, Dominique Roubaty, Kaisa Holmberg, Guy Werlen, Georg A Holländer, Nicholas R J Gascoigne, Ed Palmer (2006) <i>Nature</i> <i></i> <i></i> 444: 7120 724-729<br/>

http://publicationslist.org/e.palmer/refid67 2009-08-22T14:08:30Z

The src family kinases p56lck (Lck) and p59fyn (Fyn) are the most proximal signaling molecules to be activated downstream of the T-cell receptor. Using an inducible transgenic model, we can regulate the expression of Lck in primary T cells and ask how the signaling cascade and differentiation potential are affected by the absence or the presence of reduced levels of Lck. We show that in naïve T c...<br/><br/>Matthew Lovatt, Andrew Filby, Valentino Parravicini, Guy Werlen, Ed Palmer, Rose Zamoyska (2006) <i>Mol Cell Biol</i> <i></i> <i></i> 26: 22 8655-8665<br/>

http://publicationslist.org/e.palmer/refid93 2009-08-22T14:22:34Z

The CD3epsilon proline-rich sequence (PRS) binds to the cytosolic adaptor molecule Nck after TCR ligation. It has been proposed that this interaction is essential for immunological synapse formation and T cell activation. To assess the physiological importance of the CD3epsilon PRS, we have generated mice that lack this motif (CD3epsilon.PRS(M)). Pull-down experiments demonstrated the inability of...<br/><br/>Andrea L Szymczak, Creg J Workman, Diana Gil, Smaroula Dilioglou, Kate M Vignali, Ed Palmer, Dario A A Vignali (2005) <i>J Immunol</i> <i></i> <i></i> 175: 1 270-275<br/>

http://publicationslist.org/e.palmer/refid92 2009-08-22T14:22:34Z

The T cell receptor (TCR) can recognize a variety of cognate peptide/major histocompatibility complex (pMHC) ligands and translate their affinity into distinct cellular responses. To achieve this, the nonsignaling alphabeta heterodimer communicates ligand recognition to the CD3 signaling subunits by an unknown mechanism. In thymocytes, we found that both positive- and negative-selecting pMHC ligan...<br/><br/>Diana Gil, Adam G Schrum, Balbino Alarcón, Ed Palmer (2005) <i>J Exp Med</i> <i></i> <i></i> 201: 4 517-522<br/>

http://publicationslist.org/e.palmer/refid106 2009-08-22T14:45:06Z

Ed Palmer (2005) <i>Nat Immunol</i> 6: 1 9-10<br/>

http://publicationslist.org/e.palmer/refid94 2009-08-22T14:23:42Z

Naive T cells become programmed for clonal expansion and contraction during the early hours of antigenic signaling. Recent studies support an 'autopilot' model, wherein the commitment to proliferate and the magnitude of the proliferative response are simultaneously determined during a single, brief period of antigen exposure. Here, we have examined whether the proliferation of naive CD4+ T cells m...<br/><br/>Adam G Schrum, Ed Palmer, Laurence A Turka (2005) <i>Eur J Immunol</i> <i></i> <i></i> 35: 2 449-459<br/>

http://publicationslist.org/e.palmer/refid81 2009-08-22T14:16:15Z

We have studied the role of the T cell receptor (TCR) beta chain transmembrane and cytoplasmic domains (betaTM/Cyto) in T cell signaling. Upon antigen stimulation, T lymphocytes expressing a TCR with mutant and betaTM and Cyto domains accumulate in large numbers and are specifically defective in undergoing activation-induced cell death (AICD). The mutant TCR poorly recruits the protein adaptor Car...<br/><br/>Emma Teixeiro, Mark A Daniels, Barbara Hausmann, Adam G Schrum, Dieter Naeher, Immanuel Luescher, Margot Thome, Rafael Bragado, Ed Palmer (2004) <i>Immunity</i> <i></i> <i></i> 21: 4 515-526<br/>

http://publicationslist.org/e.palmer/refid84 2009-08-22T14:16:15Z

T cells expressing T cell receptor (TCR) complexes that lack CD3 delta, either due to deletion of the CD3 delta gene, or by replacement of the connecting peptide of the TCR alpha chain, exhibit severely impaired positive selection and TCR-mediated activation of CD8 single-positive T cells. Because the same defects have been observed in mice expressing no CD8 beta or tailless CD8 beta, we examined ...<br/><br/>Marie-Agnès Doucey, Laurence Goffin, Dieter Naeher, Olivier Michielin, Petra Baumgärtner, Philippe Guillaume, Ed Palmer, Immanuel F Luescher (2003) <i>J Biol Chem</i> <i></i> <i></i> 278: 5 3257-3264<br/>

http://publicationslist.org/e.palmer/refid82 2009-08-22T14:16:15Z

Recognition by CD8+ cytotoxic T lymphocytes (CTLs) of antigenic peptides bound to major histocompatibility class (MHC) I molecules on target cells leads to sustained calcium mobilization and CTL degranulation resulting in perforin-dependent killing. We report that beta1 and beta3 integrin-mediated adhesion to extracellular matrix proteins on target cells and/or surfaces dramatically promotes CTL d...<br/><br/>Marie-Agnès Doucey, Daniel F Legler, Mustapha Faroudi, Nicole Boucheron, Petra Baumgaertner, Dieter Naeher, Marek Cebecauer, Denis Hudrisier, Curzio Rüegg, Ed Palmer, Salvatore Valitutti, Claude Bron, Immanuel F Luescher (2003) <i>J Biol Chem</i> <i></i> <i></i> 278: 29 26983-26991<br/>

http://publicationslist.org/e.palmer/refid95 2009-08-22T14:23:42Z

It is important to understand how T-cell antigen receptor (TCR) engagement and signaling are regulated throughout an immune response. This review examines the dynamics of surface TCR expression and signaling capacity during thymic and effector T-cell development. Although the TCR can undergo vast changes in surface expression, T cells remain capable of sustaining TCR engagement for long periods of...<br/><br/>Adam G Schrum, Laurence A Turka, Ed Palmer (2003) <i>Immunol Rev</i> <i></i> <i></i> 196: 7-24<br/>

http://publicationslist.org/e.palmer/refid103 2009-08-22T14:38:49Z

Dead cells are a prominent feature of the thymic landscape as only 5% of developing thymocytes are exported as mature T cells. The remaining thymocytes die by one of two mechanisms; most thymocytes die because they are not positively selected and do not receive a survival signal, whereas a minority of thymocytes undergo T-cell receptor (TCR)-mediated apoptosis, a process known as negative selectio...<br/><br/>Ed Palmer (2003) <i>Nat Rev Immunol</i> 3: 5 383-391<br/>

http://publicationslist.org/e.palmer/refid86 2009-08-22T14:17:55Z

The application of TCR transgenic mice to transplantation immunology is hampered by the limited lines available. Recently, we reported CD4+ T cell receptor (TCR) transgenic mice specific for I-Abm12 expressed on B6.C.H-2bm12 mice. Here, we characterized rejection of skin and vascularized cardiac allografts in these mice, which we term ABM (for anti-bm12). In vivo proliferative experiments reveal t...<br/><br/>Mohamed H Sayegh, Zihao Wu, Wayne W Hancock, Peter B Langmuir, Marielena Mata, Sigrid Sandner, Koji Kishimoto, Masayuki Sho, Ed Palmer, Richard N Mitchell, Laurence A Turka (2003) <i>Am J Transplant</i> <i></i> <i></i> 3: 4 381-389<br/>

http://publicationslist.org/e.palmer/refid85 2009-08-22T14:17:55Z

We have developed an adoptive transfer model system to visualize the dynamics of alloantigen-specific CD4+ T-cell activation in vivo. Using TCR-transgenic (tg) mice reactive to I-Ab(m12), we studied the clonal expansion and differentiation of alloreactive T cells by tracking the fate of adoptively transferred TCR-tg CD4+ T cells in syngeneic mice transplanted with skin grafts expressing I-Ab(m12)....<br/><br/>Sigrid E Sandner, Alan D Salama, Stuart L Houser, Ed Palmer, Laurence A Turka, Mohamed H Sayegh (2003) <i>Am J Transplant</i> <i></i> <i></i> 3: 10 1242-1250<br/>

http://publicationslist.org/e.palmer/refid68 2009-08-22T14:08:30Z

T lymphocytes are generated in the thymus, where developing thymocytes must accept one of two fates: They either differentiate or they die. These fates are chiefly determined by signals that originate from the T cell receptor (TCR), a single receptor complex with a remarkable capacity to decide between distinct cell fates. This review explores TCR signaling in thymocytes and focuses on the kinetic...<br/><br/>Guy Werlen, Barbara Hausmann, Dieter Naeher, Ed Palmer (2003) <i>Science</i> <i></i> <i></i> 299: 5614 1859-1863<br/>

http://publicationslist.org/e.palmer/refid73 2009-08-22T14:10:31Z

Both the direct and indirect antigen presentation pathways are important mechanisms for T cell-mediated allograft rejection. Studies using knockout mice and monoclonal antibodies have demonstrated that CD4+ T cells are both necessary and sufficient for the rejection of allogeneic tissues, including skin, heart, and islet. Furthermore, combined blockade of the CD28/B7 and CD154/CD40 costimulatory p...<br/><br/>Ingrid C Rulifson, Gregory L Szot, Ed Palmer, Jeffrey A Bluestone (2002) <i>Am J Transplant</i> 2: 6 510-519<br/>

http://publicationslist.org/e.palmer/refid76 2009-08-22T14:13:41Z

The origin and specificity of alphabeta TCR(+) T cells that express CD8alphaalpha have been controversial issues. Here we provide direct evidence that precursors of functional CD8alphaalpha T cells are positively selected in the thymus in the presence of agonist self-peptides. Like conventional positive selection, this agonist selection process requires functional TCR alpha-CPM, whereas it is inde...<br/><br/>Andrew J Leishman, Laurent Gapin, Myriam Capone, Ed Palmer, H Robson MacDonald, Mitchell Kronenberg, Hilde Cheroutre (2002) <i>Immunity</i> <i></i> <i></i> 16: 3 355-364<br/>

http://publicationslist.org/e.palmer/refid102 2009-08-22T14:35:56Z

Ed Palmer (2002) <i>Nat Immunol</i> 3: 8 710-711<br/>

http://publicationslist.org/e.palmer/refid69 2009-08-22T14:08:30Z

Signal transduction in T cells is a dynamic process involving a large number of membrane and cytosolic proteins. The TCR macromolecular complex (signalosome) is initiated by receptor occupancy and becomes more elaborate over time. This review describes how 'vertical' displacement mechanisms and lateral coalescence of lipid-raft-associated scaffold proteins combine to form distinct signalosomes, wh...<br/><br/>Guy Werlen, Ed Palmer (2002) <i>Curr Opin Immunol</i> <i></i> <i></i> 14: 3 299-305<br/>

http://publicationslist.org/e.palmer/refid83 2009-08-22T14:16:15Z

To generate peripheral T cells that are both self-MHC restricted and self-MHC tolerant, thymocytes are subjected to positive and negative selection. How the TCR discriminates between positive and negative selection ligands is not well understood, although there is substantial evidence that the CD4 and CD8 coreceptors play an important role in this cell fate decision. We have previously identified ...<br/><br/>Dieter Naeher, Immanuel F Luescher, Ed Palmer (2002) <i>J Immunol</i> <i></i> <i></i> 169: 6 2964-2970<br/>

http://publicationslist.org/e.palmer/refid77 2009-08-22T14:13:41Z

Natural killer T (NKT) cells are a subset of mature alpha beta TCR(+) cells that co-express NK lineage markers. Whereas most NKT cells express a canonical Valpha14/Vbeta8.2 TCR and are selected by CD1d, a minority of NKT cells express a diverse TCR repertoire and develop independently of CD1d. Little is known about the selection requirements of CD1d-independent NKT cells. We show here that NKT cel...<br/><br/>M Capone, M Troesch, G Eberl, B Hausmann, E Palmer, H R MacDonald (2001) <i>Eur J Immunol</i> <i></i> <i></i> 31: 6 1867-1875<br/>

http://publicationslist.org/e.palmer/refid71 2009-08-22T14:08:30Z

The widely accepted kinetic proofreading theory proposes that rapid TCR dissociation from a peptide/MHC ligand allows for stimulation of early but not late T cell activation events, explaining why low-affinity TCR ligands are poor agonists. We identified a low-affinity TCR ligand which stimulated late T cell responses but, contrary to predictions from kinetic proofreading, inefficiently induced ea...<br/><br/>C Rosette, G Werlen, M A Daniels, P O Holman, S M Alam, P J Travers, N R Gascoigne, E Palmer, S C Jameson (2001) <i>Immunity</i> <i></i> <i></i> 15: 1 59-70<br/>

http://publicationslist.org/e.palmer/refid74 2009-08-22T14:11:21Z

A key role in the communication between the alphabetaTCR and the CD3/zeta complex is played by a specific motif within the connecting peptide domain of the TCR alpha chain (alpha-CPM). T cell hybridomas expressing an alpha-CPM-mutated TCR show a dramatic impairment in antigen-driven interleukin-2 production. This defect can be complemented by a calcium ionophore, indicating that activation of the ...<br/><br/>C Ulivieri, A Peter, E Orsini, E Palmer, C T Baldari (2001) <i>J Biol Chem</i> 276: 5 3574-3580<br/>

http://publicationslist.org/e.palmer/refid75 2009-08-22T14:12:05Z

Alloreactive T cell precursor frequency was measured in vivo using fluorescent dye labeling in combination with novel models based on lymphocyte activation and recovery. CFSE-labeled C57BL/6 (H-2(b)) spleen and lymph node cells were adoptively transferred to C57BL/6xDBA F(1) (H-2(b/d)) recipients, a parent-->F(1) MHC mismatch in which only donor cells respond. Recipients were sacrificed at serial ...<br/><br/>E J Suchin, P B Langmuir, E Palmer, M H Sayegh, A D Wells, L A Turka (2001) <i>J Immunol</i> 166: 2 973-981<br/>

http://publicationslist.org/e.palmer/refid70 2009-08-22T14:08:30Z

Positive selection allows thymocytes that recognize an individual's own major histocompatibility complex (self-MHC) molecules to survive and differentiate, whereas negative selection removes overtly self-reactive thymocytes. Although both forms of thymic selection are mediated by the alphabeta T-cell receptor (TCR) and require self-MHC recognition, an important question is whether they are control...<br/><br/>G Werlen, B Hausmann, E Palmer (2000) <i>Nature</i> <i></i> <i></i> 406: 6794 422-426<br/>

http://publicationslist.org/e.palmer/refid65 2009-08-22T14:06:28Z

To investigate the molecular basis that makes heterodimeric CD8alphabeta a more efficient coreceptor than homodimeric CD8alphaalpha, we used various CD8 transfectants of T1.4 T cell hybridomas, which are specific for H-2Kd, and a photoreactive derivative of the Plasmodium berghei circumsporozoite peptide PbCS 252-260 (SYIPSAEKI). We demonstrate that CD8 is palmitoylated at the cytoplasmic tail of ...<br/><br/>A Arcaro, C Grégoire, N Boucheron, S Stotz, E Palmer, B Malissen, I F Luescher (2000) <i>J Immunol</i> 165: 4 2068-2076<br/>

http://publicationslist.org/e.palmer/refid72 2009-08-22T14:09:45Z

T cell development is tightly controlled by thymic stromal cells. Alterations in stromal architecture affect T cell maturation and the development of self-tolerance. The monogenic autoimmune syndrome APECED (autoimmune-polyendocrinopathy-candidiasis-ectodermal dystrophy) is characterized by the loss of self-tolerance to multiple organs. Although mutations in the autoimmune regulator (AIRE) gene ar...<br/><br/>S Zuklys, G Balciunaite, A Agarwal, E Fasler-Kan, E Palmer, G A Holländer (2000) <i>J Immunol</i> 165: 4 1976-1983<br/>

http://publicationslist.org/e.palmer/refid63 2009-08-20T20:59:27Z

Antagonist peptides inhibit T cell responses by an unknown mechanism. By coexpressing two independent T cell receptors (TCRs) on a single T cell hybridoma, we addressed the question of whether antagonist ligands induce a dominant-negative signal that inhibits the function of a second, independent TCR. The two receptors, Valpha2Vbeta5 and Valpha2Vbeta10, restricted by H-2Kb and specific for the oct...<br/><br/>S H Stotz, L Bolliger, F R Carbone, E Palmer (1999) <i>J Exp Med</i> <i></i> <i></i> 189: 2 253-264<br/>

http://publicationslist.org/e.palmer/refid9 2009-08-18T14:07:13Z

Hybrids of New Zealand Black (NZB) and New Zealand White (NZW) mice spontaneously develop a disease similar to human systemic lupus erythematosus. MHC and non-MHC genes contribute to disease susceptibility in this murine model. Multiple studies have shown that the NZW H2z locus is strongly associated with the development of lupus-like disease in these mice. The susceptibility gene(s) within H2z is...<br/><br/>S J Rozzo, T J Vyse, C S David, E Palmer, S Izui, B L Kotzin (1999) <i>J Immunol</i> 162: 5 2623-2630<br/>

http://publicationslist.org/e.palmer/refid61 2009-08-20T20:59:27Z

The zeta-chain homodimer is a key component in the TCR complex and exerts its function through its cytoplasmic immunoreceptor-tyrosine activation motif (1). The zeta-chain extracellular (EC) domain is highly conserved; however, its functional and structural contributions to the TCR signaling have not been elucidated. We show that the EC domain of the zeta homodimer is essential for TCR surface exp...<br/><br/>B Johansson, E Palmer, L Bolliger (1999) <i>J Immunol</i> <i></i> <i></i> 162: 2 878-885<br/>

http://publicationslist.org/e.palmer/refid64 2009-08-20T21:00:40Z

The T cell receptor (TCR) is internalized following activation of protein kinase C (PKC) via a leucine (Leu)-based motif in CD3gamma. Some studies have indicated that the TCR is recycled back to the cell surface following PKC-mediated internalization. The functional state of recycled TCR and the mechanisms involved in the sorting events following PKC-induced internalization are not known. In this ...<br/><br/>J Dietrich, T Bäckström, J P Lauritsen, J Kastrup, M D Christensen, F von Bülow, E Palmer, C Geisler (1998) <i>J Biol Chem</i> 273: 37 24232-24238<br/>

http://publicationslist.org/e.palmer/refid10 2009-08-18T14:27:36Z

Unlike parental New Zealand Black (NZB) or New Zealand White (NZW) mice, (NZB x NZW)F1 mice exhibit a lupus-like disease characterized by IgG autoantibody production and severe immune complex-mediated nephritis. In studies of the genetic susceptibility to disease in this F1 model, the NZW MHC (H2z) has been strongly linked with the development of disease, and it was hypothesized that class II MHC ...<br/><br/>T J Vyse, S J Rozzo, C G Drake, V B Appel, M Lemeur, S Izui, E Palmer, B L Kotzin (1998) <i>J Immunol</i> <i></i> <i></i> 160: 6 2757-2766<br/>

http://publicationslist.org/e.palmer/refid107 2009-08-22T14:48:23Z

The two lineages of T cells, alphabeta and gammadelta, differ in their developmental requirements: only alphabeta T cells require major histocompatibility complex recognition, a process known as positive selection. The alphabeta T cell receptor (TCR), but not its gammadelta counterpart, contains a motif within the alpha-chain connecting peptide domain (alpha-CPM) that has been conserved over the l...<br/><br/>B T Bäckström, U Müller, B Hausmann, E Palmer (1998) <i>Science</i> <i></i> <i></i> 281: 5378 835-838<br/>

http://publicationslist.org/e.palmer/refid105 2009-08-22T14:44:27Z

Antigen stimulation through the T cell receptor (TCR) induces phosphorylation of the associated CD3 gamma delta epsilon- and zeta-chain cytoplasmic tails. These events lead to the induction of the intracellular signaling pathways with concomitant receptor down-regulation. The TCR is down-regulated from the cell surface by the activation of protein kinase, C (PKC) and subsequent serine phosphorylat...<br/><br/>B T Bäckström, B Rubin, A Peter, G Tiefenthaler, E Palmer (1997) <i>Eur J Immunol</i> <i></i> <i></i> 27: 6 1433-1441<br/>

http://publicationslist.org/e.palmer/refid62 2009-08-20T20:59:27Z

The zeta chain is required in the TCR complex to guarantee its surface expression and function. However, an understanding of the interaction(s) between the zeta chain and the other proteins in the TCR/CD3 has not yet been achieved. In this report, we attempt to assign a functional role to the short extracellular (EC) domain of the zeta chain by studying its unique positive charge, a lysine at posi...<br/><br/>L Bolliger, B Johansson, E Palmer (1997) <i>Mol Immunol</i> <i></i> <i></i> 34: 12-13 819-827<br/>

http://publicationslist.org/e.palmer/refid104 2009-08-22T14:44:27Z

A single amino acid residue, Gln136, located within the connecting peptide domain of Cbeta controls the ability of the alpha/beta TCR to transmit a full signal. TCRs in which this Cbeta residue is mutated to Phe, the residue found in TCR-gamma, are unresponsive to antigenic ligands. Interestingly, this Cbeta residue is either polar or charged in every species studied thus far, including the trout ...<br/><br/>B T Bäckström, B T Hausmann, E Palmer (1997) <i>J Exp Med</i> <i></i> <i></i> 186: 11 1933-1938<br/>

http://publicationslist.org/e.palmer/refid108 2009-08-22T14:48:23Z

Mutant alphabeta TCRs were generated by replacing domains of the alpha and beta chain constant regions with homologous domains from TCR delta and gamma chains, respectively. Chimeric TCRs in which the alpha chain contains TCR delta chain sequences within the connecting peptide domain are unresponsive to alloantigens and superantigens, and have defective interactions with the CD3/zeta complex. Alth...<br/><br/>B T Bäckström, E Milia, A Peter, B Jaureguiberry, C T Baldari, E Palmer (1996) <i>Immunity</i> <i></i> <i></i> 5: 5 437-447<br/>

http://publicationslist.org/e.palmer/refid11 2009-08-18T14:27:36Z

C G Drake, S J Rozzo, T J Vyse, E Palmer, B L Kotzin (1995) <i>Immunol Rev</i> <i></i> <i></i> 144: 51-74<br/>

http://publicationslist.org/e.palmer/refid12 2009-08-18T14:27:36Z

F1 progeny of New Zealand Black (NZB) and New Zealand White (NZW) mice spontaneously develop an autoimmune process remarkably similar to human systemic lupus erythematosus. Previous studies have implicated major genetic contributions from the NZW MHC and from a dominant NZB gene on chromosome 4. To identify additional NZB contributions to lupus-like disease, (NZB x SM/J)F1 x NZW backcross mice wer...<br/><br/>C G Drake, S J Rozzo, H F Hirschfeld, N P Smarnworawong, E Palmer, B L Kotzin (1995) <i>J Immunol</i> <i></i> <i></i> 154: 5 2441-2447<br/>

http://publicationslist.org/e.palmer/refid59 2009-08-20T20:56:40Z

Current models of T cell receptor (TCR) structure are generally based on the homology observed between the TCR and the immunoglobulins. Furthermore, these models have predicted the locations of framework and complementarity determining regions within the alpha- and beta-chain variable regions. In order to test the validity of these models, we have generated a series of mutations within the V beta ...<br/><br/>D L DiGiusto, E Palmer (1994) <i>Mol Immunol</i> 31: 9 693-699<br/>

http://publicationslist.org/e.palmer/refid60 2009-08-20T20:57:23Z

We have compared the transmembrane sequences of 72 vertebrate antigen receptor (mIg and TCR) polypeptides. This allowed us to identify a Conserved Antigen Receptor Transmembrane (CART) motif which is present in all antigen receptor transmembrane domains from species as far removed as cartilaginous fish. Most of the amino acids in the CART motif are polar or aromatic and may interact with other pro...<br/><br/>K S Campbell, B T Bäckström, G Tiefenthaler, E Palmer (1994) <i>Semin Immunol</i> 6: 6 393-410<br/>

http://publicationslist.org/e.palmer/refid13 2009-08-18T14:27:36Z

Lupus-like autoimmunity in (NZB x NZW)F1 mice is frequently marked by the development of a severe and fatal renal disease. Genes from both NZB and NZW parents are required for the full expression of disease. We applied a mapping technique based on polymorphism in simple sequence repeats to the analysis of (NZB x NZW)F1 x NZW backcross mice to determine the NZB genetic contribution to disease. The ...<br/><br/>C G Drake, S K Babcock, E Palmer, B L Kotzin (1994) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 91: 9 4062-4066<br/>

http://publicationslist.org/e.palmer/refid54 2009-08-20T20:54:04Z

Self-tolerance is the process by which the T cell repertoire develops without expressing self-reactive specificities. The mechanisms which functionally eliminate self-reactive T cells are clonal deletion and clonal inactivation, and both of these phenomena have been studied in T cell populations reactive to endogenous superantigens that are encoded by endogenous mouse mammary tumor proviruses (Mtv...<br/><br/>K J Gollob, E Palmer (1993) <i>J Immunol</i> <i></i> <i></i> 150: 9 3705-3712<br/>

http://publicationslist.org/e.palmer/refid58 2009-08-20T20:55:48Z

The hapten/class I MHC-specific soluble immunoregulatory molecules produced by CD8+T cells from dinitrobenzene sulfonate-primed mice express the binding specificity and serologic determinants of alpha/beta TCR. To examine the genes used to encode these soluble immunoregulatory molecules, we utilized a surface TCR expressing Ts hybridoma, which constitutively produces a DNP/Kd-specific regulatory m...<br/><br/>R L Fairchild, E Palmer, J W Moorhead (1993) <i>J Immunol</i> 150: 1 67-77<br/>

http://publicationslist.org/e.palmer/refid57 2009-08-20T20:54:47Z

Mls-1 is an endogenous superantigen that leads to in vivo deletion and in vitro stimulation of T cell receptor (TCR) V beta 6-, 7-, 8.1-, and 9-expressing cells. The MA/MyJ mouse deletes the identical set of TCR from its mature T cell repertoire; however, it does not contain Mtv-7, the murine mammary tumor provirus (MMTV), whose sag gene encodes Mls-1. Interestingly, the superantigen activity of t...<br/><br/>C K Rudy, E Kraus, E Palmer, B T Huber (1992) <i>J Exp Med</i> 175: 6 1613-1621<br/>

http://publicationslist.org/e.palmer/refid55 2009-08-20T20:54:04Z

Several murine superantigens in association with class II major histocompatibility complex proteins have been shown to cause the deletion of T cells based on the expression of particular beta-chain variable region (V beta) gene segments. We have previously shown that mice expressing the Etc-1 superantigen, encoded by an open reading frame within the 3' long terminal repeat of the endogenous mouse ...<br/><br/>K J Gollob, E Palmer (1992) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 89: 11 5138-5141<br/>

http://publicationslist.org/e.palmer/refid45 2009-08-20T20:47:46Z

A monoclonal antibody, B20.1, was generated by fusing spleen cells from a Lou rat immunized with a soluble alpha/beta T cell receptor (TcR; V alpha 2/V beta 2) to mouse myeloma cells. Analysis of a panel of V alpha 2 mRNA-expressing T cell lines, hybridomas and transfectants revealed that the B20.1 antibody was specific for murine TcR V alpha 2 chains. The V alpha 2+ T cell population was examined...<br/><br/>H Pircher, N Rebaï, M Groettrup, C Grégoire, D E Speiser, M P Happ, E Palmer, R M Zinkernagel, H Hengartner, B Malissen (1992) <i>Eur J Immunol</i> <i></i> <i></i> 22: 2 399-404<br/>

http://publicationslist.org/e.palmer/refid40 2009-08-20T20:41:24Z

Superantigens are defined by their ability to stimulate T cells based predominantly on their V beta expression and ability to delete T cells in the thymus when expressed endogenously. We show here that the expression of one endogenous superantigen, Etc-1, is controlled by the expression of the open reading frame region of the 3' long terminal repeat of the mouse mammary tumor proviral gene, Mtv-9....<br/><br/>D L Woodland, F E Lund, M P Happ, M A Blackman, E Palmer, R B Corley (1991) <i>J Exp Med</i> <i></i> <i></i> 174: 5 1255-1258<br/>

http://publicationslist.org/e.palmer/refid35 2009-08-20T20:36:05Z

Y Utsunomiya, J Bill, E Palmer, O Kanagawa (1991) <i>Immunogenetics</i> <i></i> <i></i> 33: 3 198-201<br/>

http://publicationslist.org/e.palmer/refid46 2009-08-20T20:47:46Z

A special class of self-antigens (endogenous superantigens) is capable of deleting many murine T cells on the basis of their expression of particular T-cell receptor V beta gene segments. In mice that endogenously express these antigens, tolerance is mediated in part by the clonal deletion of the relevant V beta-bearing T cells. The deletion of I-E-reactive V beta 5.2-bearing T cells is dependent ...<br/><br/>D L Woodland, M P Happ, K J Gollob, E Palmer (1991) <i>Nature</i> <i></i> <i></i> 349: 6309 529-530<br/>

http://publicationslist.org/e.palmer/refid14 2009-08-18T14:27:36Z

J P Portanova, S K Babcock, E Palmer, B L Kotzin (1991) <i>Immunol Ser</i> <i></i> <i></i> 54: 65-95<br/>

http://publicationslist.org/e.palmer/refid34 2009-08-20T20:36:05Z

The mAb MR9-4 and MR9-8 react with T cells expressing the V beta 5.1 and -5.2 chains of the TCR. T cells expressing V beta 5.1 TCR were stained by both antibodies with similar surface fluorescence intensity. For the T cell clones and hybridomas expressing V beta 5.2 TCR, staining intensity with MR9-8 varied from negative to comparable to that stained with the anti-pan V beta 5 mAb MR9-4, whereas e...<br/><br/>O Kanagawa, Y Utsunomiya, J Bill, E Palmer, M W Moore, F R Carbone (1991) <i>J Immunol</i> <i></i> <i></i> 147: 4 1307-1314<br/>

http://publicationslist.org/e.palmer/refid56 2009-08-20T20:54:04Z

The majority of endogenous superantigens in the mouse (including the Mls loci) is encoded by mouse mammary tumor proviruses (Mtv) carried in the germline. To understand the differences between the highly stimulatory viral superantigens such as Mls-1a (encoded by Mtv-7), which have biologic activity in vivo and in vitro, and the poorly stimulatory viral superantigens such as Etc-1 (encoded by Mtv-9...<br/><br/>K J Gollob, E Palmer (1991) <i>J Immunol</i> <i></i> <i></i> 147: 8 2447-2454<br/>

http://publicationslist.org/e.palmer/refid98 2009-08-22T14:27:37Z

H-2b class I-restricted, TNP-specific CTL clones were obtained by limiting dilution cloning of either short term polyclonal CTL lines or spleen cells of TNP-immunized mice directly ex vivo. Sequence analyses of mRNA coding for TCR alpha- and beta-chains of 11 clones derived from CTL lines from individual C57BL/6 mice revealed that all of them expressed unique but clearly nonrandom receptor structu...<br/><br/>B Kempkes, E Palmer, S Martin, A von Bonin, K Eichmann, B Ortmann, H U Weltzien (1991) <i>J Immunol</i> 147: 8 2467-2473<br/>

http://publicationslist.org/e.palmer/refid99 2009-08-22T14:28:59Z

H Suzuki, F Z Chung, E Palmer, T Sasaki, N Ohara, B A Taylor, J Ohara (1991) <i>Immunogenetics</i> 34: 4 252-256<br/>

http://publicationslist.org/e.palmer/refid53 2009-08-20T20:52:36Z

The effects of quantitative differences in class II cell surface expression have been difficult to address in intact animals. This study uses several lines of H-2s/s mice carrying an A beta k transgene that differ significantly in terms of class II cell surface expression. Due to inefficient chain pairing, mice carrying 60 to 65 copies of this transgene express only low levels of A alpha s/A beta ...<br/><br/>S Gilfillan, S Aiso, D Smilek, D L Woodland, E Palmer, H O McDevitt (1991) <i>J Immunol</i> 147: 12 4074-4081<br/>

http://publicationslist.org/e.palmer/refid97 2009-08-22T14:26:11Z

We have characterized a panel of monoclonal antibodies (mAb) produced by immunizing rats with two distinct soluble mouse alpha/beta T cell receptor (TcR). Fifty mAb were found to react with the corresponding surface-bound TcR. Such observations suggest that the soluble TcR molecules used as immunogen are folded in a conformation similar to the native structure. Furthermore, the binding to T cells ...<br/><br/>A Necker, N Rebaï, M Matthes, E Jouvin-Marche, P A Cazenave, P Swarnworawong, E Palmer, H R MacDonald, B Malissen (1991) <i>Eur J Immunol</i> 21: 12 3035-3040<br/>

http://publicationslist.org/e.palmer/refid101 2009-08-22T14:34:49Z

The identity of minor lymphocytes stimulating (Mls) antigens, endogenous superantigens that can activate, or induce the deletion of, large portions of the T-cell repertoire, has recently been revealed: they are encoded by mouse mammary tumor viruses (MMTV) that have integrated into the germ line as DNA proviruses. As Hans Acha-Orbea and Ed Palmer point out, Mls-mediated modulation may be only the ...<br/><br/>H Acha-Orbea, E Palmer (1991) <i>Immunol Today</i> 12: 10 356-361<br/>

http://publicationslist.org/e.palmer/refid39 2009-08-20T20:41:24Z

Clonal deletion plays a major part in the maintenance of natural self-tolerance in both normal and transgenic mice. Self antigens that are expressed in the thymus result in the physical elimination of autoreactive thymocytes at a particular stage in their development. For example, the majority V beta 6- and V beta 8.1-bearing T cells that recognize the minor lymphocyte-stimulating antigen, Mls-1a ...<br/><br/>M A Blackman, H Gerhard-Burgert, D L Woodland, E Palmer, J W Kappler, P Marrack (1990) <i>Nature</i> <i></i> <i></i> 345: 6275 540-542<br/>

http://publicationslist.org/e.palmer/refid96 2009-08-22T14:25:25Z

We have generated a rat mAb, TR310, which recognizes a determinant encoded by the murine V beta 7 gene segment of the TCR. TR310 immunoprecipitates TCR from cell lysates, co-modulates with CD3, and can be used for immunofluorescence staining of T cells. By using this antibody, we found that the average percentage of V beta 7+ peripheral T cells in Mls-1b mice was 3.8%, but only 0.8% in Mls-1a mice...<br/><br/>C Y Okada, B Holzmann, C Guidos, E Palmer, I L Weissman (1990) <i>J Immunol</i> 144: 9 3473-3477<br/>

http://publicationslist.org/e.palmer/refid15 2009-08-18T14:27:36Z

The TCR beta-chain locus of NZW mice carries an 8.8-kb deletion which encompasses the C beta 1, D beta 2, and all six J beta 2 gene segments. On a theoretical basis, the absence of D beta 2 and J beta 2 gene segments in this strain should result in a 70% reduction of the diversity of the TCR repertoire. To experimentally assess the effects of this deletion, we bred the NZW TCR beta-chain allele on...<br/><br/>D L Woodland, B L Kotzin, E Palmer (1990) <i>J Immunol</i> <i></i> <i></i> 144: 1 379-385<br/>

http://publicationslist.org/e.palmer/refid36 2009-08-20T20:36:05Z

T cells that express the T cell receptor V beta 5.2 domain react with the class II major histocompatibility complex (MHC) molecule I-E, and V beta 5.2+ T cells are deleted in mouse strains that express I-E glycoproteins. By examination of genetically defined recombinant inbred (RI) mouse strains, it was found that the deletion was dependent on the expression of I-E and one of a limited number of n...<br/><br/>D Woodland, M P Happ, J Bill, E Palmer (1990) <i>Science</i> <i></i> <i></i> 247: 4945 964-967<br/>

http://publicationslist.org/e.palmer/refid31 2009-08-20T20:36:05Z

We have previously shown that T cells bearing V beta 5+ T-cell receptors (TCRs) are frequent in B10 (H-2b) and B10.Q (H-2q) mouse strains but are rare in the congenic strain B10.BR (H-2k). Furthermore, we have found that V beta 5 bearing T cells appear to be excluded from the B10 alloresponse to I-Abm12 despite the participation of most other V beta bearing cells. To further study MHC effects on V...<br/><br/>J Bill, O Kanagawa, J Linten, Y Utsunomiya, E Palmer (1990) <i>J Mol Cell Immunol</i> <i></i> <i></i> 4: 5 269-79; discussion 279-80<br/>

http://publicationslist.org/e.palmer/refid30 2009-08-20T20:36:05Z

Three rat mAb, RR3-15, RR3-16, and RR3-18, were established by fusing spleen cells from a rat immunized with the male Ag-specific cytolytic T cell clone, OH6, to mouse myeloma cells. The mAb was identified by their capacity to focus the cytolytic activity of the OH6 CTL clone on nonspecific target cells via FcR-FcR interaction. That all three mAb recognized the OH6 TCR was confirmed by immunopreci...<br/><br/>Y Utsunomiya, J Bill, E Palmer, K Gollob, Y Takagaki, O Kanagawa (1989) <i>J Immunol</i> <i></i> <i></i> 143: 8 2602-2608<br/>

http://publicationslist.org/e.palmer/refid28 2009-08-20T20:36:05Z

We have generated an mAb, RR3-15, that recognizes murine TCRs containing the V beta 11 domain. Using this antibody to stain peripheral T cells, we have demonstrated that V beta 11-bearing T cells are largely absent from strains of mice that express the class II MHC molecule, I-E. Studies with F1 mice demonstrate that this effect is dominant, consistent with tolerance. The clonal deletion of V beta...<br/><br/>J Bill, O Kanagawa, D L Woodland, E Palmer (1989) <i>J Exp Med</i> <i></i> <i></i> 169: 4 1405-1419<br/>

http://publicationslist.org/e.palmer/refid24 2009-08-20T20:23:11Z

We have studied the genetic diversity of the TCR repertoire to the murine alloantigen I-Abm12 by generating a panel of 178 C57BL/10-derived I-Abm12-reactive T cell hybridomas. The expression of V alpha and V beta gene families was examined in this panel and the frequency of expression of V beta, but not ofV alpha, gene families differed significantly from that observed in a companion panel of rand...<br/><br/>J Bill, J Yagüe, V B Appel, J White, G Horn, H A Erlich, E Palmer (1989) <i>J Exp Med</i> <i></i> <i></i> 169: 1 115-133<br/>

http://publicationslist.org/e.palmer/refid32 2009-08-20T20:36:05Z

The I-Abm12 mutation has been used extensively to study the relationship between structure and function of murine class II major histocompatibility molecules. I-Abm12 differs from I-Ab by three amino acid replacements in the A beta chain, and the proposed structural model of the I-Abm12 molecule places these three amino acid substitutions along one of the alpha-helices where they may affect both a...<br/><br/>J Bill, F Ronchese, R N Germain, E Palmer (1989) <i>J Exp Med</i> <i></i> <i></i> 170: 3 739-750<br/>

http://publicationslist.org/e.palmer/refid48 2009-08-20T20:47:46Z

To investigate the possible function(s) of T cell receptor (TcR) gamma delta expressing lymphocytes, we generated a series of gamma delta TcR surface positive hybridomas. Spontaneous producers of IL-2 were quite common among these hybridomas, particularly those expressing a certain V delta gene or gene family (V delta M23). Several other experiments indicated that IL-2 production in these hybridom...<br/><br/>R L O'Brien, M P Happ, A Dallas, E Palmer, R Kubo, W K Born (1989) <i>Cell</i> <i></i> <i></i> 57: 4 667-674<br/>

http://publicationslist.org/e.palmer/refid29 2009-08-20T20:36:05Z

A monoclonal antibody secreting hybridoma was established by fusing spleen cells from a rat immunized with a murine T cell clone, OI11, which has I-Ab restricted specificity for the male H-Y antigen and unrestricted specificity for the minor lymphocyte stimulating antigen, Mls-1a, to the mouse myeloma P3X63AG8.653 and screening for the capacity of the hybridoma supernatants to stimulate the OI11 T...<br/><br/>O Kanagawa, E Palmer, J Bill (1989) <i>Cell Immunol</i> <i></i> <i></i> 119: 2 412-426<br/>

http://publicationslist.org/e.palmer/refid33 2009-08-20T20:36:05Z

We have examined the frequency of expression of individual alpha and beta chain V gene families in a population of immature T cells that has not been selected or tolerized. To accomplish this, we generated 519 T cell hybridomas from freshly isolated thymocytes of newborn C57BL/10 mice and subjected RNA from these hybrids to hybridization analysis with 11 V alpha, 16 V beta, C gamma and C delta pro...<br/><br/>M P Happ, E Palmer (1989) <i>Eur J Immunol</i> <i></i> <i></i> 19: 7 1317-1325<br/>

http://publicationslist.org/e.palmer/refid16 2009-08-18T14:27:36Z

Unlike parental New Zealand Black (NZB) or New Zealand White (NZW) mice, (NZB x NZW)F1 mice exhibit a lupus-like disease characterized by high serum levels of IgG anti-nuclear antibodies and a fatal immune complex glomerulonephritis. Previous results from studying [(NZB x NZW)F1 x NZB] backcross mice indicated that the NZW major histocompatibility complex (MHC) or gene(s) closely linked to this lo...<br/><br/>S K Babcock, V B Appel, M Schiff, E Palmer, B L Kotzin (1989) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 86: 19 7552-7555<br/>

http://publicationslist.org/e.palmer/refid49 2009-08-20T20:47:46Z

E Palmer, D L Woodland, M P Happ, J Bill, O Kanagawa (1989) <i>Cold Spring Harb Symp Quant Biol</i> <i></i> <i></i> 54 Pt 1: 135-145<br/>

http://publicationslist.org/e.palmer/refid47 2009-08-20T20:47:46Z

The physiological role of lymphocytes bearing the gamma delta T-cell receptor (TCR) is still unclear. A function for a subset of these cells, however, is inferred from the finding that certain gamma delta chain-bearing lymphocytes are stimulated in a receptor-dependent fashion by mycobacterial antigens. We found that hybridomas derived from such cells in newborn murine thymus not only responded to...<br/><br/>M P Happ, R T Kubo, E Palmer, W K Born, R L O'Brien (1989) <i>Nature</i> <i></i> <i></i> 342: 6250 696-698<br/>

http://publicationslist.org/e.palmer/refid43 2009-08-20T20:45:07Z

Recent evidence suggests that the major histocompatibility complex class II molecules of B lymphocytes function as signal-transducing receptors during the generation of T lymphocyte-dependent humoral immune responses. By analogy with other receptors, we postulate that perturbation of the class II molecules is coupled to the generation of intracellular second messengers through interactions involvi...<br/><br/>W F Wade, Z Z Chen, R Maki, S McKercher, E Palmer, J C Cambier, J H Freed (1989) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 86: 16 6297-6301<br/>

http://publicationslist.org/e.palmer/refid50 2009-08-20T20:47:46Z

Using a quantitative RNA hybridization method, we have identified a third murine T-cell receptor beta-chain variable (V) region gene, V beta 9, that engenders recognition of Mls-1a gene products. V beta 9-expressing T cells are clonally deleted from the periphery of mice that carry the Mls-1a allele, and the fine specificity of V beta 9+ hybridomas suggests that V beta 6-, V beta 8.1-, and V beta ...<br/><br/>M P Happ, D L Woodland, E Palmer (1989) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 86: 16 6293-6296<br/>

http://publicationslist.org/e.palmer/refid37 2009-08-20T20:36:05Z

T lymphocytes differentiate in the thymus, where functionally immature, CD4+CD8+ (double positive) thymocytes develop into functionally mature CD4+ helper cells and CD8+ cytotoxic (single positive) T cells. The thymus is the site where self-reactive T cells are negatively selected (clonally deleted) and where T cells with the capacity to recognize foreign antigens in association with self-proteins...<br/><br/>J Bill, E Palmer (1989) <i>Nature</i> <i></i> <i></i> 341: 6243 649-651<br/>

http://publicationslist.org/e.palmer/refid38 2009-08-20T20:37:13Z

Our understanding of the immune mechanisms that lead to systemic lupus erythematosus has been greatly advanced by the availability of murine models which display both serological and clinical features of the human disease. Studies have demonstrated that CD4+ T cells are required for the full expression of disease in these mice. (NZB X NZW)F1 mice exhibit a lupus-like disease (elevated levels of Ig...<br/><br/>B L Kotzin, L R Herron, S K Babcock, J P Portanova, E Palmer (1989) <i>Clin Immunol Immunopathol</i> 53: 2 Pt 2 S35-S46<br/>

http://publicationslist.org/e.palmer/refid52 2009-08-20T20:50:15Z

The data presented here indicated that both membrane Ig and IL-4 receptors transduce signals across the plasma membrane of quiescent B cells, which results in the induction of c-fos and c-myc proto-oncogene mRNA expression. Monoclonal anti-Ig antibodies with specificity for mu, delta, or kappa chains, regardless of mitogenicity, induced increased c-fos and c-myc mRNA expression with kinetics and m...<br/><br/>M J Klemsz, L B Justement, E Palmer, J C Cambier (1989) <i>J Immunol</i> 143: 3 1032-1039<br/>

http://publicationslist.org/e.palmer/refid21 2009-08-20T20:23:11Z

Antigen receptors on most T-cells are heterodimeric glycoproteins, comprised of an alpha chain and a beta chain. These chains are encoded by discontiguous variable (V), diversity (D) and joining (J) gene segments that rearrange to produce a contiguous and functional alpha or beta chain gene. To investigate the size and diversity of the germline repertoire of alpha-chain gene segments, we have char...<br/><br/>J Yague, M Blackman, W Born, P Marrack, J Kappler, E Palmer (1988) <i>Nucleic Acids Res</i> <i></i> <i></i> 16: 23 11355-11364<br/>

http://publicationslist.org/e.palmer/refid17 2009-08-18T14:27:36Z

In contrast to parental New Zealand Black (NZB) or New Zealand White (NZW) mice, (NZB x NZW)F1 mice exhibit a lupus-like disease characterized by high levels of immunoglobulin G (IgG) antinuclear antibodies in their serum and a fatal immune-complex glomerulonephritis. At least three gene loci have been identified in NZW mice that could potentially contribute to a T cell-dependent autoimmune diseas...<br/><br/>B L Kotzin, E Palmer (1988) <i>Am J Med</i> <i></i> <i></i> 85: 6A 29-31<br/>

http://publicationslist.org/e.palmer/refid42 2009-08-20T20:43:13Z

The T-cell-activating protein TAP is a murine phosphatidylinositol-anchored glycoprotein whose expression is controlled by the Ly-6 locus. Previous studies have suggested an important role for this protein in physiological T-cell activation. Using oligonucleotide probes, we have now isolated a cDNA clone whose predicted sequence would encode a protein with an NH2-terminal sequence identical to tha...<br/><br/>H Reiser, J Coligan, E Palmer, B Benacerraf, K L Rock (1988) <i>Proc Natl Acad Sci U S A</i> 85: 7 2255-2259<br/>

http://publicationslist.org/e.palmer/refid44 2009-08-20T20:45:07Z

The TCR alpha/beta variable element V beta 17a is expressed in all strains of mice carrying the V beta a complex in which about half of the V beta elements have been lost due to a large deletion. The mAb KJ23a detects V beta 17a containing alpha/beta receptors in these mice. Mouse strains with the V beta b complex carry a full complement of V beta genes including a structural gene for V beta 17 (V...<br/><br/>T Wade, J Bill, P C Marrack, E Palmer, J W Kappler (1988) <i>J Immunol</i> <i></i> <i></i> 141: 6 2165-2167<br/>

http://publicationslist.org/e.palmer/refid18 2009-08-20T20:16:00Z

We report the molecular cloning of a human gene MER-2 located on chromosome 11 that encodes a cell surface antigen which is polymorphic on red blood cells. An essential element of the cloning strategy was cotransfection-induced linkage of pSV2-neo, which encodes resistance to the antibiotic G418, to the human MER-2 gene. An important feature of the pSV2-neo construct is that the same gene (the tra...<br/><br/>J Bill, E Palmer, C Jones (1987) <i>Somat Cell Mol Genet</i> <i></i> <i></i> 13: 5 553-561<br/>

http://publicationslist.org/e.palmer/refid22 2009-08-20T20:23:11Z

The receptors of two T cell hybridomas that recognize class I and class II major histocompatibility complex (MHC) molecules, respectively, have been compared. In both cases these receptors are hybrid molecules formed as a result of cellular fusion. The receptors contain the same alpha chain, contributed by the tumor cell fusion partner, and related beta chains, contributed by the normal T cell com...<br/><br/>M Blackman, J Yagüe, R Kubo, D Gay, C Coleclough, E Palmer, J Kappler, P Marrack (1986) <i>Cell</i> <i></i> <i></i> 47: 3 349-357<br/>

http://publicationslist.org/e.palmer/refid23 2009-08-20T20:23:11Z

The genetic control of the cytotoxic T-cell response to the male histocompatibility antigen, H-Y, was analyzed in BALB/cKe(C) and SJL/J(J) which are both nonresponders. However, the (C X J)F1 hybrid is a responder. Therefore, two dominant complementing genes are involved. Analysis of a set of (C X J) recombinant inbred (RI) lines reveals that these two complementing gene products are a restricting...<br/><br/>R Epstein, G Sham, J Womack, J Yagüe, E Palmer, M Cohn (1986) <i>J Exp Med</i> <i></i> <i></i> 163: 4 759-773<br/>

http://publicationslist.org/e.palmer/refid25 2009-08-20T20:23:11Z

The kinetics and order of rearrangements in the gene complex encoding T-cell-receptor beta chains were studied by Southern blot hybridization in a collection of hybridomas derived from fetal thymocytes at various stages of ontogeny (day 14 to day 17). Our results show a steady increase in the frequency of rearranged beta complexes during this period and suggest that these rearrangements occur with...<br/><br/>W Born, J Yagüe, E Palmer, J Kappler, P Marrack (1985) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 82: 9 2925-2929<br/>

http://publicationslist.org/e.palmer/refid27 2009-08-20T20:26:06Z

To isolate DNA segments specific to chromosome band 14q11, which has been implicated in a number of human T-cell malignancies, a genomic DNA library was prepared from a variant cell subline of the human lymphoblastic KE37 cell line. This subline (KE37-R) bears a t(8;14) (q24;q11) translocation, and the breakpoint on the resulting chromosome 8q+ has been located at the 3' end of the third c-myc exo...<br/><br/>D Mathieu-Mahul, J F Caubet, A Bernheim, M Mauchauffé, E Palmer, R Berger, C J Larsen (1985) <i>EMBO J</i> 4: 13A 3427-3433<br/>

http://publicationslist.org/e.palmer/refid26 2009-08-20T20:24:27Z

Two allelic forms of the T cell antigen receptor alpha chain gene were discerned by restriction fragment length polymorphism (RFLP) employing the T cell antigen receptor alpha chain probe pGA5, and the restriction enzyme Bgl II. Analysis revealed that the polymorphic fragments are detected by a probe specific for the constant region exon of the T cell antigen receptor alpha chain gene. Furthermore...<br/><br/>M L Hoover, J Marks, J Chipman, E Palmer, P Stastny, J D Capra (1985) <i>J Exp Med</i> 162: 3 1087-1092<br/>

http://publicationslist.org/e.palmer/refid20 2009-08-20T20:23:11Z

Three independent T cell hybridomas were isolated that have identical specificities for antigen and products of the major histocompatibility complex (MHC). All three react with the same clone-specific antireceptor antibody, and Southern blots show all three contain the same rearranged alpha and beta genes. Variants of one of these hybridomas, DO-11.10, were isolated that had lost the ability to re...<br/><br/>J Yagüe, J White, C Coleclough, J Kappler, E Palmer, P Marrack (1985) <i>Cell</i> <i></i> <i></i> 42: 1 81-87<br/>

http://publicationslist.org/e.palmer/refid19 2009-08-20T20:16:00Z

The genes encoding the alpha chain of the human T-cell receptor have been mapped to chromosome 14, the chromosome on which the human immunoglobulin heavy chain locus resides. Thus, genes encoding two different classes of antigen receptor are present on the same chromosome. Furthermore, breaks involving chromosome 14 are frequently seen in tumors of T-cell origin. The potential relation of these ch...<br/><br/>C Jones, H G Morse, F T Kao, A Carbone, E Palmer (1985) <i>Science</i> <i></i> <i></i> 228: 4695 83-85<br/>

http://publicationslist.org/e.palmer/refid7 2009-08-18T14:01:58Z

We have investigated the structure of the murine Y chromosome by first developing a novel method for specifically cloning Y-encoded DNA and then generating a library enriched for Y-specific DNA sequences. Three randomly chosen Y DNA clones were studied and found to share several interesting properties: all three are members of small Y-specific multisequence families; all three are mouse-specific; ...<br/><br/>E E Lamar, E Palmer (1984) <i>Cell</i> 37: 1 171-177<br/>

http://publicationslist.org/e.palmer/refid8 2009-08-18T14:04:11Z

The T-cell receptor has been studied intensely over the past 10 years in an effort to understand the molecular basis for major histocompatibility complex (MHC) restricted antigen recognition. The use of anti-receptor monoclonal antibodies to isolate and characterize the receptor from human and murine T-cell clones has shown that the protein consists of two disulphide-linked glycopeptides, alpha an...<br/><br/>G K Sim, J Yagüe, J Nelson, P Marrack, E Palmer, A Augustin, J Kappler (1984) <i>Nature</i> 312: 5996 771-775<br/>

http://publicationslist.org/e.palmer/refid6 2009-08-18T13:59:53Z

M Masurekar, E Palmer, B I Ono, J M Wilhelm, F Sherman (1981) <i>J Mol Biol</i> <i></i> <i></i> 147: 3 381-390<br/>

http://publicationslist.org/e.palmer/refid4 2009-08-18T13:59:53Z

E Palmer, J M Wilhelm, F Sherman (1979) <i>J Mol Biol</i> <i></i> <i></i> 128: 1 107-110<br/>

http://publicationslist.org/e.palmer/refid5 2009-08-18T13:59:53Z

E Palmer, J M Wilhelm, F Sherman (1979) <i>Nature</i> <i></i> <i></i> 277: 5692 148-150<br/>

http://publicationslist.org/e.palmer/refid111 2009-08-22T14:55:42Z

A total of 358 lys2 mutants of Saccharomyces cerevisiae have been characterized for suppressibility by the following suppressors: UAA and UAG suppressors that insert tyrosine, serine or leucine; a putative UGA suppressor; an omnipotent suppressor SUP46; and a frameshift suppressor SUF1-1. In addition, the lys2 mutants were examined for phenotypic suppression by the aminoglycoside antibiotic paromo...<br/><br/> Chattoo, Palmer, Ono, Sherman (1979) <i>Genetics</i> 93: 1 67-79<br/>

http://publicationslist.org/e.palmer/refid3 2009-08-18T13:59:53Z

Previous work from our laboratory has demonstrated that a subclass of the aminoglycoside antibiotics, those containing the drug fragment paromamine, stimulates mistranslation in cell-free protein-synthesizing systems derived from eucaryotic cells. We report here experiments which show that the ciliate Tetrahymena thermophila (formerly T. pyriformis, syngen 1) is sensitive to the paromamine-contain...<br/><br/>E Palmer, J M Wilhelm (1978) <i>Cell</i> <i></i> <i></i> 13: 2 329-334<br/>

http://publicationslist.org/e.palmer/refid1 2009-08-18T13:53:41Z

R factor proteins are synthesized in R factor-containing Escherichia coli minicells. Half of this protein remained associated with the minicell membrane upon lysis of the minicells. Over 90% of the membrane-associated protein was extracted by sodium lauryl sarcosinate, suggesting a location of these proteins in the inner membrane. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of these ...<br/><br/>S B Levy, L McMurry, E Palmer (1974) <i>J Bacteriol</i> 120: 3 1464-1471<br/>

http://publicationslist.org/e.palmer/refid2 2009-08-18T13:56:42Z

E Palmer, F Waldman, W Dewitt (1974) <i>Yale J Biol Med</i> 47: 2 82-85<br/>

http://publicationslist.org/e.palmer/refid51 2009-08-20T20:48:15Z

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http://publicationslist.org/e.palmer/refid41 2009-08-20T20:41:45Z

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