Giuseppe Bardi's Publications List

Immunological properties of Andean starch films are independent of their nanometric roughness and stiffness.

2015-03-20T10:00:56Z

Starch is a natural material extracted from roots, seeds, stems and tubers of different plants. It can be processed as a thermoplastic to produce a variety promising products for biomedical applications, including foams, sheets and films. In the present work, we investigated the immunological properties of microfilms prepared with starches extracted from six different types of Andean potatoes and ...

F G Torres, O P Troncoso, O Gamucci, S Corvaglia, V Brunetti, G Bardi (2015) International journal of biological macromolecules 75: 460-466

Biomedical Nanoparticles: Overview of Their Surface Immune-Compatibility

2014-03-18T08:40:59Z

Gamucci O, Bertero A. Gagliardi M, Bardi* G. (2014) Coatings 1: 4 139-159

Surface functionalisation regulates polyamidoamine dendrimer toxicity on blood-brain barrier cells and the modulation of key inflammatory receptors on microglia.

2014-03-18T08:41:23Z

Dendrimers are branched polymers with spherical morphology. Their tuneable chemistry and surface modification make them valuable nanomaterials for biomedical applications. In view of possible dendrimer uses as brain-aimed nanocarriers, the authors studied amine- and lipid-functionalised (G4) polyamidoamine (PAMAM) biocompatibility with cell population forming the blood-brain barrier (BBB). Both am...

Alice Bertero, Adriano Boni, Mauro Gemmi, Mariacristina Gagliardi, Angelo Bifone, Giuseppe Bardi* (2014) Nanotoxicology 8: 2 158-168

Detection of fluorescent nanoparticle interactions with primary immune cell subpopulations by flow cytometry.

2014-07-08T09:10:59Z

Gamucci O, Bertero A, Malvindi M, Sabella S, Pompa PP, Mazzolai B, Bardi* G. (2014) Journal of Visualized Experiments 85:

Cerium dioxide nanoparticles selectively up-regulate C-C chemokine receptor 2 and CD16 expression on human monocytes

2015-03-20T10:04:12Z

Gamucci O, Bardi G (2014) Euro Nanotox Letters 5: 1 1-16

Novel siRNA delivery strategy: a new "strand" in CNS translational medicine?

2014-03-18T08:29:05Z

RNA interference has been envisaged as a powerful tool for molecular and clinical investigation with a great potential for clinical applications. In recent years, increased understanding of cancer biology and stem cell biology has dramatically accelerated the development of technology for cell and gene therapy in these areas. This paper is a review of the most recent report of innovative use of si...

Lisa Gherardini, Giuseppe Bardi, Mariangela Gennaro, Tommaso Pizzorusso (2014) Cellular and molecular life sciences : CMLS 71: 1 1-20

Cross-desensitization of CCR1, but not CCR2, following activation of the formyl peptide receptor FPR1.

2014-07-08T09:12:41Z

The cross-regulation of G protein-coupled receptors (GPCRs) plays an important role in the immune response. Studies from several laboratories have suggested that a hierarchy of sensitivities to cross-desensitization exists for the chemoattractant GPCRs. We carried out experiments to study the capacity of the formyl peptide receptor-1 (FPR1) to desensitize chemokine receptors CCR1 and CCR2. Our res...

Filip Bednar, Changcheng Song, Giuseppe Bardi, William Cornwell, Thomas J Rogers (2014) Journal of immunology (Baltimore, Md. : 1950) 192: 11 5305-5313

Negligible particle-specific toxicity mechanism of silver nanoparticles: The role of Ag(+) ion release in the cytosol.

2015-03-20T10:00:56Z

Toxicity of silver nanoparticles (AgNPs) is supported by many observations in literature, but no mechanism details have been proved yet. Here we confirm and quantify the toxic potential of fully characterized AgNPs in HeLa and A549 cells. Notably, through a specific fluorescent probe, we demonstrate the intracellular release of Ag(+) ions in living cells after nanoparticle internalization, showing...

Valeria De Matteis, Maria Ada Malvindi, Antonio Galeone, Virgilio Brunetti, Elisa De Luca, Sachin Kote, Prakash Kshirsagar, Stefania Sabella, Giuseppe Bardi, Pier Paolo Pompa (2014) Nanomedicine : nanotechnology, biology, and medicine :

Targeted drug delivery across biological barriers using polymer nanoparticles.

2014-03-18T08:46:08Z

Gagliardi MC, Bardi G, Gamucci O, Mazzolai B. (2013) Hot Topics in Therapeutic Delivery

In Vivo Distribution and Toxicity of PAMAM Dendrimers in the Central Nervous System Depend on Their Surface Chemistry.

2014-03-18T08:41:42Z

Dendrimers have been described as one of the most tunable and therefore potentially applicable nanoparticles both for diagnostics and therapy. Recently, in order to realize drug delivery agents, most of the effort has been dedicated to the development of dendrimers that could internalize into the cells and target specific intracellular compartments in vitro and in vivo. Here, we describe cell inte...

Lorenzo Albertazzi, Lisa Gherardini, Marco Brondi, Sebastian Sulis Sato, Angelo Bifone, Tommaso Pizzorusso, Gian Michele Ratto, Giuseppe Bardi* (2013) Mol Pharm 10: 1 249-260

Functionalized carbon nanotubes in the brain: cellular internalization and neuroinflammatory responses.

2014-03-18T08:29:05Z

The potential use of functionalized carbon nanotubes (f-CNTs) for drug and gene delivery to the central nervous system (CNS) and as neural substrates makes the understanding of their in vivo interactions with the neural tissue essential. The aim of this study was to investigate the interactions between chemically functionalized multi-walled carbon nanotubes (f-MWNTs) and the neural tissue followin...

Giuseppe Bardi, Antonio Nunes, Lisa Gherardini, Katie Bates, Khuloud T Al-Jamal, Claire Gaillard, Maurizio Prato, Alberto Bianco, Tommaso Pizzorusso, Kostas Kostarelos (2013) PloS one 8: 11

Selective targeting capability acquired with a protein corona adsorbed on the surface of 1,2-dioleoyl-3-trimethylammonium propane/DNA nanoparticles.

2014-03-18T08:29:05Z

A possible turning point in drug delivery has been recently reached: the protein shell, which covers nanocarriers in vivo, can be used for targeting. Here, we show that nanoparticles can acquire a selective targeting capability with a protein corona adsorbed on the surface. We demonstrate that lipid particles made of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and DNA, upon interaction with h...

Giulio Caracciolo, Francesco Cardarelli, Daniela Pozzi, Fabrizio Salomone, Giuseppe Maccari, Giuseppe Bardi, Anna Laura Capriotti, Chiara Cavaliere, Massimiliano Papi, Aldo Laganà (2013) ACS applied materials & interfaces 5: 24 13171-13179

Safety of carbon nanotubes for neuronal tissue

2014-03-18T08:43:54Z

Giuseppe Bardi* (2012) Nanomedicine and the nervous system, Nanoscience Applied to Health and Medicine 3-16

Chapter 9: Neurological System

2012-07-13T09:19:40Z

Sandra Ceccatelli, Giuseppe Bardi* (2012) Adverse Effects of Engineered Nanomaterials 157-168

Multiwalled carbon nanotube antennas induce effective plasmid DNA transfection of bacterial cells

2012-07-13T09:26:33Z

Raffa V, Vittorio O, Costa M, Ziaei A, Nitodas S, Riggio C, Al-Jamal K, Gherardini L, Bardi G, Pizzorusso T, Karachalios T, Cuschieri A. (2012) Journal of Nanoneuroscience 2: 1 56-62

Polymeric nanocarriers for controlled and enhanced delivery of therapeutic agents to the CNS.

2012-09-04T14:10:57Z

Polymeric nanocarriers are versatile structures that can be engineered to obtain high drug loading, good delivery yields and tunable release kinetics. Moreover, the particle surface can be modified for selective targeting of organs or tissues. In particular, polymeric nanocarriers can be conjugated with functional groups promoting translocation through the blood-brain barrier, thus providing a pro...

Mariacristina Gagliardi, Giuseppe Bardi, Angelo Bifone (2012) Ther Deliv 3: 7 875-887

Enhanced bioactivity of internally functionalized cationic dendrimers with PEG cores.

2013-01-12T14:04:23Z

Hybrid dendritic-linear block copolymers based on a 4-arm poly(ethylene glycol) (PEG) core were synthesized using an accelerated AB(2)/CD(2) dendritic growth approach through orthogonal amine/epoxy and thiol-yne chemistries. The biological activity of these 4-arm and the corresponding 2-arm hybrid dendrimers revealed an enhanced, dendritic effect with an exponential increase in cell internalizatio...

Lorenzo Albertazzi, Frauke M Mickler, Giovanni M Pavan, Fabrizio Salomone, Giuseppe Bardi, Mariangela Panniello, Elizabeth Amir, Taegon Kang, Kato L Killops, Christoph Bräuchle, Roey J Amir, Craig J Hawker (2012) Biomacromolecules 13: 12 4089-4097

A novel chimeric cell-penetrating peptide with membrane-disruptive properties for efficient endosomal escape.

2013-01-12T14:04:29Z

Efficient endocytosis into a wide range of target cells and low toxicity make the arginine-rich Tat peptide (Tat(11): YGRKKRRQRRR, residues 47-57 of HIV-1 Tat protein) an excellent transporter for delivery purposes. Unfortunately, molecules taken up by endocytosis undergo endosomal entrapment and possible metabolic degradation. Escape from the endosome is therefore actively researched. In this con...

Fabrizio Salomone, Francesco Cardarelli, Mariagrazia Di Luca, Claudia Boccardi, Riccardo Nifosì, Giuseppe Bardi, Lorenzo Di Bari, Michela Serresi, Fabio Beltram (2012) J Control Release 163: 3 293-303

PKC{zeta} mediates {micro}-opioid receptor-induced cross-desensitization of CCR5.

2011-04-12T11:42:12Z

We have previously shown that the μ-opioid receptor (MOR) is capable of mediating cross-desensitization of several chemokine receptors including CCR5, but the biochemical mechanism of this process has not been fully elucidated. We have carried out a series of functional and biochemical studies and found that the mechanism of MOR-induced cross-desensitization of CCR5 involves the activation of PKC...

Changcheng Song, Rahil T Rahim, Penelope C Davey, Filip Bednar, Giuseppe Bardi, Lily Zhang, Ning Zhang, Joost J Oppenheim, Thomas J Rogers (2011) J Biol Chem :

Functional motor recovery from brain ischemic insult by carbon nanotube-mediated siRNA silencing.

2011-11-05T14:33:19Z

Stroke is the second cause of death worldwide with ischemic stroke accounting for 80% of all stroke insults. Caspase-3 activation contributes to brain tissue loss and downstream biochemical events that lead to programmed cell death after traumatic brain injury. Alleviation of symptoms following ischemic neuronal injury can be potentially achieved by either genetic disruption or pharmacological inh...

Khuloud T Al-Jamal, Lisa Gherardini, Giuseppe Bardi, Antonio Nunes, Chang Guo, Cyrill Bussy, M Antonia Herrero, Alberto Bianco, Maurizio Prato, Kostas Kostarelos, Tommaso Pizzorusso (2011) Proc Natl Acad Sci U S A 108: 27 10952-10957

SiO2 NPs: Promising Candidates for Drug and Gene Delivery

2014-07-08T09:16:20Z

Giuseppe Bardi* (2011) Drug Delivery Letters 1: 1 9-12

Carbon nanotube-mediated wireless cell permeabilization: drug and gene uptake.

2012-07-13T08:54:39Z

This work aims to exploit the 'antenna' properties of multiwalled carbon nanotubes (MWCNTs). They can be used to induce cell permeabilization in order to transfer drugs (normally impermeable to cell membranes) both in in vitro and in vivo models.

Vittoria Raffa, Lisa Gherardini, Orazio Vittorio, Giuseppe Bardi, Afshin Ziaei, Tommaso Pizzorusso, Cristina Riggio, Stephanos Nitodas, Theodoros Karachalios, Khuloud T Al-Jamal, Kostas Kostarelos, Mario Costa, Alfred Cuschieri (2011) Nanomedicine (Lond) 6: 10 1709-1718

Carbon nanotubes and neurons: biocompatibility concerns

2014-03-18T08:42:49Z

Giuseppe Bardi* (2011) Journal of Nanonscience Letters 1: 3 166-171

The biocompatibility of amino functionalized CdSe/ZnS quantum-dot-Doped SiO(2) nanoparticles with primary neural cells and their gene carrying performance.

2014-03-18T08:42:17Z

Nanoparticles have an enormous potential for the development of applications in biomedicine such as gene or drug delivery. We developed and characterized NH(2) functionalized CdSe/ZnS quantum dot (QD)-doped SiO(2) nanoparticles (NPs) with both imaging and gene carrier capabilities. We show that QD-doped SiO(2) NPs are internalized by primary cortical neural cells without inducing cell death in vit...

Giuseppe Bardi*, Maria Ada Malvindi, Lisa Gherardini, Mario Costa, Pier Paolo Pompa, Roberto Cingolani, Tommaso Pizzorusso (2010) Biomaterials :

In vitro and in vivo biocompatibility testing of functionalized carbon nanotubes.

2013-10-10T08:22:58Z

The explosive growth of nanotechnology in the last years has led to dramatic innovations in pharmacology, and it is revolutioning the development of biologically active compounds. Carbon nanotubes (CNTs) are widely explored for biomedical applications such as intracellular transporters for (bio)molecules, and represent promising future tools for efficient and safe cell therapy. Due to their nanosc...

Gianni Ciofani, Vittoria Raffa, Orazio Vittorio, Alfred Cuschieri, Tommaso Pizzorusso, Mario Costa, Giuseppe Bardi (2010) Methods Mol Biol 625: 67-83

Adipocytes differentiation in the presence of Pluronic F127-coated carbon nanotubes.

2014-03-18T08:43:16Z

Carbon nanotubes are considered important nanodevices in biomedicine, and several applications in drug and gene delivery in different organs and tissues are presently under investigation. Among them very little attention has been dedicated to white adipose tissue (WAT), despite its wide distribution and endocrine role, which could potentially be used to release therapeutic agents. An important pre...

Giuseppe Bardi*, Orazio Vittorio, Margherita Maffei, Tommaso Pizzorusso, Mario Costa (2009) Nanomedicine 5: 4 378-381

Pluronic-coated carbon nanotubes do not induce degeneration of cortical neurons in vivo and in vitro.

2014-03-18T08:43:30Z

Carbon nanotubes (CNTs) are nanodevices with important potential applications in biomedicine such as drug and gene delivery. Brain diseases with no current therapy could be candidates for CNT-based therapies. Little is known about toxicity of CNTs and of their dispersion factors in the brain. Here we show that multiwall CNTs (MWCNTs) coated with Pluronic F127 (PF127) surfactant can be injected in ...

Giuseppe Bardi*, Paola Tognini, Gianni Ciofani, Vittoria Raffa, Mario Costa, Tommaso Pizzorusso (2009) Nanomedicine 5: 1 96-104

The obesity and inflammatory marker haptoglobin attracts monocytes via interaction with chemokine (C-C motif) receptor 2 (CCR2).

2010-01-08T13:57:04Z

ABSTRACT: BACKGROUND: Obesity is a chronic low inflammatory state. In the obesity condition the white adipose tissue (WAT) is massively infiltrated with monocytes/macrophages, and the nature of the signals recruiting these inflammatory cells has yet to be fully elucidated. Haptoglobin (Hp) is an inflammatory marker and its expression is induced in the WAT of obese subjects. In an effort to elucida...

Margherita Maffei, Marcella Funicello, Teresa Vottari, Olimpia Gamucci, Mario Costa, Simonetta Lisi, Alessandro Viegi, Osele Ciampi, Giuseppe Bardi, Paolo Vitti, Aldo Pinchera, Ferruccio Santini (2009) BMC Biol 7: 1

Bi-directional heterologous desensitization between the major HIV-1 co-receptor CXCR4 and the kappa-opioid receptor.

2009-05-28T12:33:19Z

We previously characterized multiple interactions between chemokine and opioid G protein-coupled receptors (GPCR), and we found both mu and delta-opioid receptors cross-desensitize CCR1, CCR2, CCR5, but not CXCR4. Here we report that the kappa-opioid receptor (KOR) is able to cross-desensitize CXCR4, and this phenomenon is bi-directional. Chemotactic responses by KOR activation are diminished with...

Matthew J Finley, Xiaohong Chen, Guiseppe Bardi, Penny Davey, Ellen B Geller, Lily Zhang, Martin W Adler, Thomas J Rogers (2008) J Neuroimmunol 197: 2 114-123

Human immunodeficiency virus gp120-induced apoptosis of human neuroblastoma cells in the absence of CXCR4 internalization.

2009-05-28T12:33:19Z

The chemokine receptor CXCR4 functions as human immunodeficiency virus (HIV)-1 coreceptor and is involved in acquired immunodeficiency virus (AIDS) neuropathogenesis. CXCR4 is expressed by most cell types in the brain, including microglia, astrocytes, and neurons. Studies have shown that the HIV envelope protein gp120 binds to neuronal CXCR4 and activates signal transduction pathways leading to ap...

Giuseppe Bardi, Rajarshi Sengupta, Muhammad Z Khan, Jeegar P Patel, Olimpia Meucci (2006) J Neurovirol 12: 3 211-218

Modulation of neuronal CXCR4 by the micro-opioid agonist DAMGO.

2009-05-28T12:33:19Z

The chemokine receptor CXCR4 regulates neuronal survival and differentiation and is involved in a number of pathologies, including cancer and human immunodeficiency virus (HIV). Recent data suggest that chemokines act in concert with neurotransmitters and neuropeptides, such as opioids. This study aimed to determine whether mu-opioid agonists alter the effect of CXCL12 (the specific CXCR4 ligand) ...

Jeegar P Patel, Rajarshi Sengupta, Giuseppe Bardi, Muhammad Z Khan, Anna Mullen-Przeworski, Olimpia Meucci (2006) J Neurovirol 12: 6 492-500

Vav-dependent and vav-independent phosphatidylinositol 3-kinase activation in murine B cells determined by the nature of the stimulus.

2009-05-28T12:33:19Z

We show in this study that B cell activation following high avidity ligation of IgM or coligation of membrane Ig with CD19 elicits similar levels of Ca(2+) flux using different mechanisms. Each form of activation requires the function of Vav and PI3K. However, Vav regulates Ca(2+) flux independently of PI3K following anti-IgM cross-linking. By contrast, Vav function is essential for PI3K activatio...

Elena Vigorito, Giuseppe Bardi, Janet Glassford, Eric W-F Lam, Elizabeth Clayton, Martin Turner (2004) J Immunol 173: 5 3209-3214

Rho kinase is required for CCR7-mediated polarization and chemotaxis of T lymphocytes.

2009-05-28T12:33:19Z

We studied the role of Rho kinase and extracellular signal-regulated kinase (ERK)-2 in the polarization and migration of T lymphocytes in response to the CCR7 ligands EBI1 ligand chemokine (ELC; CCL19) and secondary lymphoid-tissue chemokine (SLC; CCL21). Both Rho kinase protein isoforms are expressed in T lymphocytes. Inhibition of the Rho kinases with Y-27632 strongly inhibited SLC- and ELC-indu...

Giuseppe Bardi, Verena Niggli, Pius Loetscher (2003) FEBS Lett 542: 1-3 79-83

A crucial role for the p110delta subunit of phosphatidylinositol 3-kinase in B cell development and activation.

2009-05-28T12:33:19Z

Mice lacking the p110delta catalytic subunit of phosphatidylinositol 3-kinase have reduced numbers of B1 and marginal zone B cells, reduced levels of serum immunoglobulins, respond poorly to immunization with type II thymus-independent antigen, and are defective in their primary and secondary responses to thymus-dependent antigen. p110delta(-/-) B cells proliferate poorly in response to B cell rec...

Elizabeth Clayton, Giuseppe Bardi, Sarah E Bell, David Chantry, C Peter Downes, Alexander Gray, Lisa A Humphries, David Rawlings, Helen Reynolds, Elena Vigorito, Martin Turner (2002) J Exp Med 196: 6 753-763

Eotaxin is a natural antagonist for CCR2 and an agonist for CCR5.

2009-05-28T12:33:19Z

Eotaxin is a potent inducer of eosinophil chemotaxis and was considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. This study shows that eotaxin also interacts with CCR2 and CCR5 and can, thus, affect the responses of monocytes, which express both receptors. In human monocytes pretreatment with eotaxin decreased r...

P Ogilvie, G Bardi, I Clark-Lewis, M Baggiolini, M Uguccioni (2001) Blood 97: 7 1920-1924

The T cell chemokine receptor CCR7 is internalized on stimulation with ELC, but not with SLC.

2009-05-28T12:33:19Z

ELC and SLC are potent agonists for CCR7, a receptor of up-most importance for the regulation of the homing and traffic of lymphocytes into and within secondary lymphoid tissues. We have studied the effects of both chemokines on receptor re-distribution in T lymphocytes and other CCR7-bearing cells by flow cytometry and by assessing receptor mediated functions. In this paper we show that ELC and S...

G Bardi, M Lipp, M Baggiolini, P Loetscher (2001) Eur J Immunol 31: 11 3291-3297

The ligands of CXC chemokine receptor 3, I-TAC, Mig, and IP10, are natural antagonists for CCR3.

2009-05-28T12:33:19Z

Th1 and Th2 lymphocytes express a different repertoire of chemokine receptors (CCRs). CXCR3, the receptor for I-TAC (interferon-inducible T cell alpha-chemoattractant), Mig (monokine induced by gamma-interferon), and IP10 (interferon-inducible protein 10), is expressed preferentially on Th1 cells, whereas CCR3, the receptor for eotaxin and several other CC chemokines, is characteristic of Th2 cell...

P Loetscher, A Pellegrino, J H Gong, I Mattioli, M Loetscher, G Bardi, M Baggiolini, I Clark-Lewis (2001) J Biol Chem 276: 5 2986-2991

Ornithine decarboxylase activity during development of cerebellar granule neurons.

2009-05-28T12:33:19Z

Ornithine decarboxylase (ODC), the key enzyme for polyamine biosynthesis, dramatically decreases in activity during normal cerebellar development, in parallel with the progressive differentiation of granule neurons. We have studied whether a similar pattern is displayed by cerebellar granule neurons during survival and differentiation in culture. We report that when granule cells were kept in vitr...

M Sparapani, M Virgili, G Bardi, M Tregnago, B Monti, M Bentivogli, A Contestabile (1998) J Neurochem 71: 5 1898-1904