http://publicationslist.org/data/j.a.gil/atom.xml José Gilhttp://publicationslist.org/j.a.gil$basepathfavicon.icoRecent additions to José Gil's PublicationsList.org pagehttp://publicationslist.org/publications.png2015-05-26T11:51:58Z http://publicationslist.org/j.a.gil/refid70 2015-05-26T11:16:56Z

Despite current remediation efforts, arsenic contamination in water sources is still a major health problem, highlighting the need for new approaches. In this work, strains of the nonpathogenic and highly arsenic-resistant bacterium Corynebacterium glutamicum were used as inexpensive tools to accumulate inorganic arsenic, either as arsenate (As(V)) or arsenite (As(III)) species. The assays made us...<br/><br/>Almudena F Villadangos, Efrén Ordóñez, Brandán Pedre, Joris Messens, Jose A Gil, Luis M Mateos (2014) <i>Applied microbiology and biotechnology</i> <i></i> <i></i> 98: 24 10143-10152<br/>

http://publicationslist.org/j.a.gil/refid68 2012-09-28T17:23:37Z

Resistance to arsenite (As(III)) by cells is generally accomplished by arsenite efflux permeases from Acr3 or ArsB unrelated families. We analyzed the function of three Acr3 proteins from Corynebacterium glutamicum, CgAcr3-1, CgAcr3-2, and CgAcr3-3. CgAcr3-1 conferred the highest level of As(III) resistance and accumulation in vivo. CgAcr3-1 was also the most active when everted membranes vesicles...<br/><br/>Almudena F Villadangos, Hsueh-Liang Fu, Jose A Gil, Joris Messens, Barry P Rosen, Luis M Mateos (2012) <i>J Biol Chem</i> <i></i> <i></i> 287: 1 723-735<br/>

http://publicationslist.org/j.a.gil/refid69 2012-09-28T17:23:37Z

Although bacteria are considered the simplest life forms, we are now slowly unraveling their cellular complexity. Surprisingly, not only do bacterial cells have a cytoskeleton but also the building blocks are not very different from the cytoskeleton that our own cells use to grow and divide. Nonetheless, despite important advances in our understanding of the basic physiology of certain bacterial m...<br/><br/>Michal Letek, María Fiuza, Almudena F Villadangos, Luís M Mateos, José A Gil (2012) <i>Int J Cell Biol</i> <i></i> <i></i> 2012: <br/>

http://publicationslist.org/j.a.gil/refid67 2012-04-14T15:15:30Z

Arsenate reductases (ArsCs) evolved independently as a defence mechanism against toxic arsenate. In the genome of Corynebacterium glutamicum, there are two arsenic resistance operons (ars1 and ars2) and four potential genes coding for arsenate reductases (Cg_ArsC1, Cg_ArsC2, Cg_ArsC1' and Cg_ArsC4). Using knockout mutants, in vitro reconstitution of redox pathways, arsenic measurements and enzyme ...<br/><br/>Almudena F Villadangos, Karolien Van Belle, Khadija Wahni, Veronica Tamu Dufe, Sofia Freitas, Haneen Nur, Sandra De Galan, José A Gil, Jean-Francois Collet, Luis M Mateos, Joris Messens (2011) <i>Mol Microbiol</i> <i></i> <i></i> 82: 4 998-1014<br/>

http://publicationslist.org/j.a.gil/refid64 2011-03-29T11:29:39Z

A novel method for the retention of arsenate [As(V)] combining time-controlled solid-phase extraction with living bacterial biomass is presented. As(V) retention was carried out by exposing the extractant, consisting of a living double-mutant of Corynebacterium glutamicum strain ArsC1-C2, to the sample for a retention time of 1-7min, before the arsenic distribution equilibrium between the sample s...<br/><br/>A F Villadangos, E Ordóñez, M I Muñoz, I M Pastrana, M Fiuza, J A Gil, L M Mateos, A J Aller (2010) <i>Talanta</i> <i></i> <i></i> 80: 3 1421-1427<br/>

http://publicationslist.org/j.a.gil/refid66 2011-03-29T11:29:39Z

Corynebacteria grow by wall extension at the cell poles, with DivIVA being an essential protein orchestrating cell elongation and morphogenesis. DivIVA is considered a scaffolding protein able to recruit other proteins and enzymes involved in polar peptidoglycan biosynthesis. Partial depletion of DivIVA induced overexpression of cg3264, a previously uncharacterized gene that encodes a novel coiled...<br/><br/>Maria Fiuza, Michal Letek, Jade Leiba, Almudena F Villadangos, José Vaquera, Isabelle Zanella-Cléon, Luís M Mateos, Virginie Molle, José A Gil (2010) <i>J Biol Chem</i> <i></i> <i></i> 285: 38 29387-29397<br/>

http://publicationslist.org/j.a.gil/refid61 2010-06-02T07:14:05Z

Corynebacterium glutamicum is a rod-shaped actinomycete with a distinct model of peptidoglycan synthesis during cell elongation, which takes place at the cell poles and is sustained by the essential protein DivIVA(CG) (C. glutamicum DivIVA). This protein contains a short conserved N-terminal domain and two coiled-coil regions: CC1 and CC2. Domain deletions and chimeric versions of DivIVA were used...<br/><br/>Michal Letek, María Fiuza, Efrén Ordóñez, Almudena F Villadangos, Klas Flärdh, Luís M Mateos, José A Gil (2009) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 297: 1 110-116<br/>

http://publicationslist.org/j.a.gil/refid60 2010-06-01T19:07:27Z

We identified the first enzymes that use mycothiol and mycoredoxin in a thiol/disulfide redox cascade. The enzymes are two arsenate reductases from Corynebacterium glutamicum (Cg_ArsC1 and Cg_ArsC2), which play a key role in the defense against arsenate. In vivo knockouts showed that the genes for Cg_ArsC1 and Cg_ArsC2 and those of the enzymes of the mycothiol biosynthesis pathway confer arsenate ...<br/><br/>Efrén Ordóñez, Karolien Van Belle, Goedele Roos, Sandra De Galan, Michal Letek, Jose A Gil, Lode Wyns, Luis M Mateos, Joris Messens (2009) <i>J Biol Chem</i> 284: 22 15107-15116<br/>

http://publicationslist.org/j.a.gil/refid62 2010-06-02T07:14:39Z

The coiled-coil protein DivIVA is a determinant of apical growth and hyphal branching in Streptomyces coelicolor. We have investigated the properties of this protein and the involvement of different domains in its essential function and subcellular targeting. In S. coelicolor cell extracts, DivIVA was present as large oligomeric complexes that were not strongly membrane associated. The purified pr...<br/><br/>Sheng-Bing Wang, Stuart Cantlay, Niklas Nordberg, Michal Letek, José A Gil, Klas Flärdh (2009) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 297: 1 101-109<br/>

http://publicationslist.org/j.a.gil/refid63 2010-06-02T07:15:40Z

Members of the Acr3 family of arsenite permeases confer resistance to trivalent arsenic by extrusion from cells, with members in every phylogenetic domain. In this study bacterial Acr3 homologues from Alkaliphilus metalliredigens and Corynebacterium glutamicum were cloned and expressed in Escherichia coli. Modification of a single cysteine residue that is conserved in all analyzed Acr3 homologues ...<br/><br/>Hseuh-Liang Fu, Yuling Meng, Efrén Ordóñez, Almudena F Villadangos, Hiranmoy Bhattacharjee, José A Gil, Luís M Mateos, Barry P Rosen (2009) <i>J Biol Chem</i> <i></i> <i></i> 284: 30 19887-19895<br/>

http://publicationslist.org/j.a.gil/refid55 2010-06-01T19:16:09Z

The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVA protein (DivIVA(Cg)) resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a...<br/><br/>Michal Letek, Efrén Ordóñez, José Vaquera, William Margolin, Klas Flärdh, Luis M Mateos, José A Gil (2008) <i>J Bacteriol</i> <i></i> <i></i> 190: 9 3283-3292<br/>

http://publicationslist.org/j.a.gil/refid56 2010-06-01T19:04:34Z

Corynebacterium glutamicum contains four serine/threonine protein kinases (STPKs) named PknA, PknB, PknG, and PknL. Here we present the first biochemical and comparative analysis of all four C. glutamicum STPKs and investigate their potential role in cell shape control and peptidoglycan synthesis during cell division. In vitro assays demonstrated that, except for PknG, all STPKs exhibited autokina...<br/><br/>Maria Fiuza, Marc J Canova, Isabelle Zanella-Cléon, Michel Becchi, Alain J Cozzone, Luís M Mateos, Laurent Kremer, José A Gil, Virginie Molle (2008) <i>J Biol Chem</i> <i></i> <i></i> 283: 26 18099-18112<br/>

http://publicationslist.org/j.a.gil/refid54 2010-06-02T07:18:27Z

Bacterial cell growth and cell division are highly complicated and diversified biological processes. In most rod-shaped bacteria, actin-like MreB homologues produce helicoidal structures along the cell that support elongation of the lateral cell wall. An exception to this rule is peptidoglycan synthesis in the rod-shaped actinomycete Corynebacterium glutamicum, which is MreB-independent. Instead, ...<br/><br/>Michal Letek, María Fiuza, Efrén Ordóñez, Almudena F Villadangos, Astrid Ramos, Luís M Mateos, José A Gil (2008) <i>Antonie Van Leeuwenhoek</i> <i></i> <i></i> 94: 1 99-109<br/>

http://publicationslist.org/j.a.gil/refid59 2010-06-01T19:06:45Z

The Mur ligases play an essential role in the biosynthesis of bacterial cell-wall peptidoglycan and thus represent attractive targets for the design of novel antibacterials. These enzymes catalyze the stepwise formation of the peptide moiety of the peptidoglycan disaccharide peptide monomer unit. MurC is responsible of the addition of the first residue (L-alanine) onto the nucleotide precursor UDP...<br/><br/>Maria Fiuza, Marc J Canova, Delphine Patin, Michal Letek, Isabelle Zanella-Cléon, Michel Becchi, Luís M Mateos, Dominique Mengin-Lecreulx, Virginie Molle, José A Gil (2008) <i>J Biol Chem</i> <i></i> <i></i> 283: 52 36553-36563<br/>

http://publicationslist.org/j.a.gil/refid57 2010-06-01T19:05:31Z

Expression of the genes for resistance to heavy metals and metalloids is transcriptionally regulated by the toxic ions themselves. Members of the ArsR/SmtB family of small metalloregulatory proteins respond to transition metals, heavy metals, and metalloids, including As(III), Sb(III), Cd(II), Pb(II), Zn(II), Co(II), and Ni(II). These homodimeric repressors bind to DNA in the absence of inducing m...<br/><br/>Efrén Ordóñez, Saravanamuthu Thiyagarajan, Jeremy D Cook, Timothy L Stemmler, José A Gil, Luís M Mateos, Barry P Rosen (2008) <i>J Biol Chem</i> 283: 37 25706-25714<br/>

http://publicationslist.org/j.a.gil/refid58 2010-06-01T19:17:38Z

Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and cell shape in Streptomyces.<br/><br/>Antje Marie Hempel, Sheng-bing Wang, Michal Letek, José A Gil, Klas Flärdh (2008) <i>J Bacteriol</i> <i></i> <i></i> 190: 22 7579-7583<br/>

http://publicationslist.org/j.a.gil/refid1 2010-06-02T07:16:20Z

Analysis of the complete genome sequence of Corynebacterium glutamicum indicated that, in addition to ftsI, there are eight proteins with sequence motifs that are strongly conserved in penicillin binding proteins (PBPs): four genes that code for high-molecular-weight (HMW)-PBPs (PBP1a, PBP1b, PBP2a and PBP2b), two genes encoding low-molecular-weight PBPs (PBP4 and PBP4b) and two probable beta-lact...<br/><br/>Noelia Valbuena, Michal Letek, Efrén Ordóñez, Juan Ayala, Richard A Daniel, José A Gil, Luis M Mateos (2007) <i>Mol Microbiol</i> <i></i> <i></i> 66: 3 643-657<br/>

http://publicationslist.org/j.a.gil/refid2 2010-06-02T07:17:40Z

The natural resistance mechanisms of corynebacteria to respond to the environments containing high levels of arsenic were successfully adopted to develop inexpensive and selective extractants for submicrogram amounts of arsenic. Kinetic and equilibrium characteristics were evaluated, and a preliminary exploration of the capability of these strains to be used for arsenic speciation was also made in...<br/><br/>J C Feo, E Ordoñez, M Letek, M A Castro, M I Muñoz, J A Gil, L M Mateos, A J Aller (2007) <i>Water Res</i> <i></i> <i></i> 41: 3 531-542<br/>

http://publicationslist.org/j.a.gil/refid53 2010-06-01T21:25:00Z

Of the five promoters detected for the ftsZ gene in Corynebacterium glutamicum, three were located within the coding region of the upstream ftsQ gene and two within the intergenic ftsQ-ftsZ region. The most distant ftsZ promoter showed activity in Escherichia coli and controlled high-level transcriptional expression of ftsZ in C. glutamicum. Quantitative Western blotting showed that all five promo...<br/><br/>Michal Letek, Efrén Ordóñez, María Fiuza, Pilar Honrubia-Marcos, José Vaquera, José A Gil, Dolores Castro, Luís M Mateos (2007) <i>Int Microbiol</i> <i></i> <i></i> 10: 4 271-282<br/>

http://publicationslist.org/j.a.gil/refid6 2010-06-01T19:15:24Z

The genes involved in gluconate catabolism (gntP and gntK) in Corynebacterium glutamicum are scattered in the chromosome, and no regulatory genes are apparently associated with them, in contrast with the organization of the gnt operon in Escherichia coli and Bacillus subtilis. In C. glutamicum, gntP and gntK are essential genes when gluconate is the only carbon and energy source. Both genes contai...<br/><br/>Michal Letek, Noelia Valbuena, Angelina Ramos, Efrén Ordóñez, José A Gil, Luís M Mateos (2006) <i>J Bacteriol</i> <i></i> <i></i> 188: 2 409-423<br/>

http://publicationslist.org/j.a.gil/refid5 2010-06-01T19:01:01Z

In Corynebacterium glutamicum, as in many Gram-positive bacteria, the cell division gene ftsI is located at the beginning of the dcw cluster, which comprises cell division- and cell wall-related genes. Transcriptional analysis of the cluster revealed that ftsI is transcribed as part of a polycistronic mRNA, which includes at least mraZ, mraW, ftsL, ftsI and murE, from a promoter that is located up...<br/><br/>Noelia Valbuena, Michal Letek, Angelina Ramos, Juan Ayala, Diana Nakunst, Joern Kalinowski, Luis M Mateos, José A Gil (2006) <i>Microbiology</i> <i></i> <i></i> 152: Pt 8 2491-2503<br/>

http://publicationslist.org/j.a.gil/refid4 2010-06-01T21:26:12Z

Arsenic is an extremely toxic metalloid that, when present in high concentrations, severely threatens the biota and human health. Arsenic contamination of soil, water, and air is a global growing environmental problem due to leaching from geological formations, the burning of fossil fuels, wastes generated by the gold mining industry present in uncontrolled landfills, and improper agriculture or m...<br/><br/>Luís M Mateos, Efrén Ordóñez, Michal Letek, José A Gil (2006) <i>Int Microbiol</i> <i></i> <i></i> 9: 3 207-215<br/>

http://publicationslist.org/j.a.gil/refid3 2007-12-14T17:32:53Z

The actinomycete Corynebacterium amycolatum is a saprophytic bacterium usually associated with the human skin, but it is at present considered an emergent pathogen as it is isolated from nosocomial settings from samples of immunosuppressed patients. The conventional method to distinguish C. amycolatum from closely related species is mainly based on phenotypic or chemotaxonomic studies. We develope...<br/><br/>M Letek, E Ordóñez, I Fernández-Natal, J A Gil, L M Mateos (2006) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 265: 2 256-263<br/>

http://publicationslist.org/j.a.gil/refid9 2007-12-14T17:32:53Z

Our goal in this work was to overexpress the essential cell division FtsZ protein from Corynebacterium glutamicum (Brevibacterium lactofermentum) (FtsZCG) in Escherichia coli to produce anti-FtsZCG polyclonal antibodies. Previous results from our laboratory showed that ftsZCG was not expressed in E. coli in a sufficient amount to purify FtsZCG. However, when ftsZCG (without upstream sequences) was...<br/><br/>María Pilar Honrubia-Marcos, Angelina Ramos, José A Gil (2005) <i>Can J Microbiol</i> <i></i> <i></i> 51: 1 85-89<br/>

http://publicationslist.org/j.a.gil/refid8 2010-06-01T18:59:51Z

Corynebacterium glutamicum is a Gram-positive bacterium that lacks the cell division FtsA protein and actin-like MreB proteins responsible for determining cylindrical cell shape. When the cell division ftsZ gene from C. glutamicum (ftsZ(Cg)) was cloned in different multicopy plasmids, the resulting constructions could not be introduced into C. glutamicum; it was assumed that elevated levels of Fts...<br/><br/>Angelina Ramos, Michal Letek, Ana Belén Campelo, José Vaquera, Luis M Mateos, José A Gil (2005) <i>Microbiology</i> <i></i> <i></i> 151: Pt 8 2563-2572<br/>

http://publicationslist.org/j.a.gil/refid7 2010-06-01T21:28:51Z

Corynebacterium glutamicum is able to grow in media containing up to 12 mM arsenite and 500 mM arsenate and is one of the most arsenic-resistant microorganisms described to date. Two operons (ars1 and ars2) involved in arsenate and arsenite resistance have been identified in the complete genome sequence of Corynebacterium glutamicum. The operons ars1 and ars2 are located some distance from each ot...<br/><br/>Efrén Ordóñez, Michal Letek, Noelia Valbuena, José A Gil, Luis M Mateos (2005) <i>Appl Environ Microbiol</i> <i></i> <i></i> 71: 10 6206-6215<br/>

http://publicationslist.org/j.a.gil/refid11 2007-12-14T17:34:13Z

The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse several steps in the formation of the precursors for peptidoglycan synthesis in C. ...<br/><br/>Angelina Ramos, Maria P Honrubia, Daniel Vega, Juan A Ayala, Ahmed Bouhss, Dominique Mengin-Lecreulx, José A Gil (2004) <i>Res Microbiol</i> <i></i> <i></i> 155: 3 174-184<br/>

http://publicationslist.org/j.a.gil/refid10 2007-12-14T17:34:13Z

The Aspergillus nidulans gene xlnA coding for the fungal xylanase X22 has been cloned and expressed in two heterologous bacterial hosts: Streptomyces lividans and Brevibacterium lactofermentum. Streptomyces strains yielded 10 units/ml of xylanase when the protein was produced with its own signal peptide, and 19 units/ml when its signal peptide was replaced by the one for xylanase Xys1 from Strepto...<br/><br/>M Díaz, S A I Adham, D Ramón, J A Gil, R I Santamaría (2004) <i>Appl Microbiol Biotechnol</i> <i></i> <i></i> 65: 4 401-406<br/>

http://publicationslist.org/j.a.gil/refid16 2007-12-14T17:37:46Z

The biosynthesis of the aromatic polyene macrolide antibiotic candicidin, produced by Streptomyces griseus IMRU 3570, begins with a p-aminobenzoic acid (PABA) molecule which is activated to PABA-CoA and used as starter for the head-to-tail condensation of four propionate and 14 acetate units to produce a polyketide molecule to which the deoxysugar mycosamine is attached. Using the gene coding for ...<br/><br/>J A Gil, A B Campelo-Diez (2003) <i>Appl Microbiol Biotechnol</i> <i></i> <i></i> 60: 6 633-642<br/>

http://publicationslist.org/j.a.gil/refid15 2007-12-14T17:34:13Z

Over the past 15 years the biosynthetic gene clusters for numerous bioactive polyketides have been intensively studied and recently this work has been extended to the antifungal polyene macrolides. These compounds consist of large macrolactone rings that have a characteristic series of conjugated double bonds, as well as an exocyclic carboxyl group and an unusual mycosamine sugar. The biosynthetic...<br/><br/>J F Aparicio, P Caffrey, J A Gil, S B Zotchev (2003) <i>Appl Microbiol Biotechnol</i> <i></i> <i></i> 61: 3 179-188<br/>

http://publicationslist.org/j.a.gil/refid12 2010-06-01T18:59:04Z

In Brevibacterium lactofermentum, as in many Gram-positive bacteria, a divIVA gene is located downstream from the dcw cluster of cell-division- and cell-wall-related genes. This gene (divIVA(BL)) is mostly expressed during exponential growth, and the protein encoded, DivIVA(BL,) bears some sequence similarity to antigen 84 (Ag84) from mycobacteria and was detected with monoclonal antibodies agains...<br/><br/>Angelina Ramos, María Pilar Honrubia, Noelia Valbuena, José Vaquera, Luis M Mateos, José A Gil (2003) <i>Microbiology</i> <i></i> <i></i> 149: Pt 12 3531-3542<br/>

http://publicationslist.org/j.a.gil/refid13 2007-12-14T17:34:13Z

A 20-kDa Brevibacterium lactofermentum protein was detected when purifying the His-tagged FtsZBL. The protein was identified by matrix-assisted laser desorption/ionisation time of flight as the inorganic pyrophosphatase encoded by the ppa gene, which is present as a single copy in the genome of Corynebacterium glutamicum. The ppa gene was cloned from B. lactofermentum chromosomal DNA by polymerase...<br/><br/>Angelina Ramos, Sirin A I Adham, José A Gil (2003) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 225: 1 85-92<br/>

http://publicationslist.org/j.a.gil/refid14 2007-12-14T17:34:13Z

The tyrosinase operon ( melC) from Streptomyces glaucescens was cloned and functionally expressed in Brevibacterium lactofermentum and Corynebacterium glutamicum under the control of the promoter of the kan gene from Tn 5. Recombinant corynebacterial cells containing the tyrosinase operon produced melanin on agar plates and in liquid culture when supplemented with copper and tyrosine. A conjugativ...<br/><br/>Sirin A I Adham, Sonia Rodríguez, Angelina Ramos, Ramón I Santamaría, José A Gil (2003) <i>Arch Microbiol</i> <i></i> <i></i> 180: 1 53-59<br/>

http://publicationslist.org/j.a.gil/refid18 2010-06-01T18:58:00Z

A 205 kb DNA region from Streptomyces griseus IMRU 3570, including the candicidin biosynthetic gene cluster, was cloned and partially sequenced. Analysis of the sequenced DNA led to identification of genes encoding part of a modular polyketide synthase (PKS), genes for thioesterase, macrolactone ring modification, mycosamine biosynthesis and attachment to the macrolide ring, candicidin export and ...<br/><br/>Ana Belén Campelo, José A Gil (2002) <i>Microbiology</i> <i></i> <i></i> 148: Pt 1 51-59<br/>

http://publicationslist.org/j.a.gil/refid20 2007-12-14T17:37:46Z

The three ORFs (YFIH, ORF5 and ORF6) located downstream of the cell division genes ftsQ and ftsZ in Brevibacterium lactofermentum were disrupted by single homologous recombination events between internal fragments of the corresponding genes and the chromosomal sequences. The phenotypes of the disrupted mutants were similar to that of the wild type, suggesting that these genes are dispensable for g...<br/><br/>M P Honrubia, A Ramos, J A Gil (2001) <i>Mol Genet Genomics</i> <i></i> <i></i> 265: 6 1022-1030<br/>

http://publicationslist.org/j.a.gil/refid19 2010-06-01T21:30:25Z

A xylanolytic strain of Brevibacterium lactofermentum containing the Streptomyces halstedii His-tagged xysA gene was generated. The new strain contains DNA derived from S. halstedii, expresses xylanolytic activity, and was obtained by an integrative process mediated by a conjugative plasmid targeted to a dispensable chromosomal region located downstream from the essential cell division gene ftsZ. ...<br/><br/>S A Adham, A B Campelo, A Ramos, J A Gil (2001) <i>Appl Environ Microbiol</i> <i></i> <i></i> 67: 12 5425-5430<br/>

http://publicationslist.org/j.a.gil/refid17 2007-12-14T17:37:46Z

The xylanase ( xysA) and the cellulase ( celA1) genes from Streptomyces halstedii JM8 were cloned into Escherichia coli/ Brevibacterium lactofermentum shuttle vectors and successfully expressed in both hosts when placed downstream from the kanamycin resistance promoter (Pkan) from Tn 5 but not when under the control of their own promoters. Xylanase was secreted into the culture media of B. lactofe...<br/><br/>S A Adham, P Honrubia, M Díaz, J M Fernández-Abalos, R I Santamaría, J A Gil (2001) <i>Arch Microbiol</i> <i></i> <i></i> 177: 1 91-97<br/>

http://publicationslist.org/j.a.gil/refid21 2007-12-14T17:37:46Z

The ftsZ gene was cloned from the chromosomal DNA of Brevibacterium lactofermentum by the polymerase chain reaction (PCR) using two oligonucleotides designed from two conserved regions found in most of the previously cloned and sequenced ftsZ genes from other microorganisms. ftsZ is a single-copy gene in corynebacteria and is located downstream from ftsQ and murC, indicating linkage between genes ...<br/><br/>M P Honrubia, F J Fernández, J A Gil (1998) <i>Mol Gen Genet</i> <i></i> <i></i> 259: 1 97-104<br/>

http://publicationslist.org/j.a.gil/refid22 2007-12-14T17:37:46Z

The Brevibacterium lactofermentum EF-P gene, encoding the elongation factor protein P, was cloned and sequenced. According to DNA sequence analysis of this gene, the B. lactofermentum EF-P protein consists of 187 amino acids with a calculated molecular weight of 20,584. Southern hybridization of an internal fragment of the EF-P gene from B. lactofermentum with chromosomal DNAs from different micro...<br/><br/>A Ramos, J R Macias, J A Gil (1997) <i>Gene</i> <i></i> <i></i> 198: 1-2 217-222<br/>

http://publicationslist.org/j.a.gil/refid24 2007-12-14T17:37:46Z

Two plasmid cloning vectors (pULMJ55 and pULMJ95) were constructed for Brevibacterium lactofermentum using the origin of replication of the endogenous plasmid pBL1. Plasmid pULMJ55 is a replacement vector with transcriptional terminators from the B. lactofermentum trp operon flanking the BglII cloning sites. Religation of the BglII digested vector without insert creates a 376 bp perfect palindrome...<br/><br/>R F Cadenas, C Fernández-González, J F Martín, J A Gil (1996) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 137: 1 63-68<br/>

http://publicationslist.org/j.a.gil/refid23 2007-12-14T17:37:46Z

The mobilization of plasmids from gram-negative Escherichia coli to gram-positive Brevibacterium lactofermentum, mediated by P-type transfer functions, was used to construct disrupted mutants blocked specifically in the homoserine branch of the aspartate pathway. The mutant strain B. lactofermentum R31 showed an efficiency of conjugal transfer two to three orders of magnitude higher than that of t...<br/><br/>C Fernández-González, J A Gil, L M Mateos, A Schwarzer, A Schäfer, J Kalinowski, A Pühler, J F Martín (1996) <i>Appl Microbiol Biotechnol</i> <i></i> <i></i> 46: 5-6 554-558<br/>

http://publicationslist.org/j.a.gil/refid26 2007-12-14T17:37:46Z

The amy gene of Streptomyces griseus was not expressed in Escherichia coli cells due to the lack of recognition of the amy promoter by the E. coli RNA polymerase, as confirmed by using promoter-probe vectors. The expression of the amy gene in E. coli was detected only when the promoter-less gene was placed under the control of the lacZ promoter and was dependent on the level of IPTG added to the m...<br/><br/>T Vigal, J F Martin, J A Gil (1994) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 118: 3 259-263<br/>

http://publicationslist.org/j.a.gil/refid25 2010-06-01T19:14:14Z

The minimal region for autonomous replication of pBL1, a 4.5-kb cryptic plasmid of Brevibacterium lactofermentum ATCC 13869 that has been used to construct a variety of corynebacterium vectors, was shown to be contained on a 1.8-kb HindII-SphI DNA fragment. This region contains two open reading frames (ORFs) (ORF1 and ORF5) which are essential for pBL1 replication in B. lactofermentum. Accumulatio...<br/><br/>C Fernandez-Gonzalez, R F Cadenas, M F Noirot-Gros, J F Martin, J A Gil (1994) <i>J Bacteriol</i> <i></i> <i></i> 176: 11 3154-3161<br/>

http://publicationslist.org/j.a.gil/refid28 2007-12-14T17:37:46Z

The nucleotide (nt) sequence of the gene (pab) encoding p-aminobenzoic acid (PABA) synthase, a key enzyme in the biosynthesis of candicidin by Streptomyces griseus IMRU3570, was determined and an open reading frame (ORF) of 2171 nt was found. The predicted amino acid sequence demonstrated extensive sequence identity with PABA synthases (Pab) from Gram-negative Enterobacteria. The protein encoded b...<br/><br/>L M Criado, J F Martín, J A Gil (1993) <i>Gene</i> <i></i> <i></i> 126: 1 135-139<br/>

http://publicationslist.org/j.a.gil/refid27 2007-12-14T17:37:46Z

The codon usage (CU) of 34 genes from the closely related species, Brevibacterium lactofermentum and Corynebacterium glutamicum (BLCG), was analysed and compared with that of 23 genes from other Brevibacterium and Corynebacterium species. The G+C content of the BLCG genes ranged from 50 to 62%. A wider range was found in other corynebacterial genes (25-71%). The G+C contents of non-coding regions ...<br/><br/>M Malumbres, J A Gil, J F Martín (1993) <i>Gene</i> <i></i> <i></i> 134: 1 15-24<br/>

http://publicationslist.org/j.a.gil/refid29 2007-12-14T17:37:46Z

The alpha-amylase gene (amy) from Streptomyces griseus IMRU 3570 and the beta-galactosidase gene (lac) from S. lividans were subcloned into Brevibacterium lactofermentum or B. lactofermentum/Escherichia coli shuttle vectors. The amy gene was not expressed in B. lactofermentum from its own promoter but was efficiently expressed when the promoter of the kanamycin resistance gene (kan) was inserted u...<br/><br/>R F Cadenas, J A Gil, J F Martín (1992) <i>Appl Microbiol Biotechnol</i> <i></i> <i></i> 38: 3 362-369<br/>

http://publicationslist.org/j.a.gil/refid33 2007-12-14T17:37:46Z

Several multicopy promoter-probe plasmid vectors have been constructed that replicate in Brevibacterium lactofermentum and related coryneform amino acid-producing bacteria. Transcriptional activity is detected by the expression of a promoter-less aminoglycoside phosphotransferase gene (kan) derived from transposon Tn5; expression of this gene confers kanamycin resistance in B. lactofermentum. An e...<br/><br/>R F Cadenas, J F Martín, J A Gil (1991) <i>Gene</i> <i></i> <i></i> 98: 1 117-121<br/>

http://publicationslist.org/j.a.gil/refid32 2007-12-14T17:37:46Z

A gene, amy, encoding an alpha-amylase, was cloned on a 4.8 kb Sau3A fragment from the DNA of Streptomyces griseus IMRU3570. The gene was localized to a 2.27 kb fragment by subcloning and deletion mapping experiments. The gene contained an open reading frame (ORF) of 1698 nucleotides that encoded a protein of 566 amino acids with a deduced Mr of 59713 Da. Dot-blot analysis revealed that the copy n...<br/><br/>T Vigal, J A Gil, A Daza, M D García-González, J F Martín (1991) <i>Mol Gen Genet</i> <i></i> <i></i> 225: 2 278-288<br/>

http://publicationslist.org/j.a.gil/refid31 2007-12-14T17:37:46Z

Five different mutations were introduced into the leader peptide region of the alpha-amylase gene of Streptomyces griseus IMRU 3570. A mutation which increased the positive charge of the N-terminal region of the leader peptide enhanced the secretion of alpha-amylase by two- to threefold. Replacement of the native promoter of the amylase gene by the promoter of the Tn5 neo gene or by the promoter o...<br/><br/>T Vigal, J A Gil, A Daza, M D García-González, P Villadas, J F Martín (1991) <i>Mol Gen Genet</i> <i></i> <i></i> 231: 1 88-96<br/>

http://publicationslist.org/j.a.gil/refid30 2007-12-14T17:37:46Z

The product of the saf gene of Streptomyces griseus ATCC10137 mediated an increase in the production of several extracellular enzymes and retarded the formation of pigments and spores in Streptomyces [Daza et al., Mol. Gen. Genet. 222 (1990) 384-392]. A promoter upstream from saf was identified by subcloning a DNA fragment in the promoter probe pIJ486. Using the Escherichia coli-Brevibacterium lac...<br/><br/>A Daza, J F Martín, T Vigal, J A Gil (1991) <i>Gene</i> <i></i> <i></i> 108: 1 63-71<br/>

http://publicationslist.org/j.a.gil/refid36 2007-12-14T17:37:46Z

A p-aminobenzoic synthase gene (pabS) from Streptomyces griseus IMRU 3570 involved in candicidin production was used as probe to find new aromatic polyene producing Streptomyces strains. The pab gene hybridizes with 6 out of 16 Streptomyces strains, and those strains which hybridize turned out to be polyene producers. Such strains were never before described as polyene producers.<br/><br/>J A Gil, L M Criado, T Alegre, J F Martín (1990) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 58: 1 15-18<br/>

http://publicationslist.org/j.a.gil/refid34 2007-12-14T17:37:46Z

Three different bld mutants from S. griseus ATCC 10137 were isolated by nitrosoguanidine mutagenesis. They simultaneously lost the capability of antibiotic production and the formation of pigments. The three bld mutants were differently affected by different carbon sources. Two of these mutants showed a high efficiency of transformation with several plasmid vectors, in contrast to the low efficien...<br/><br/>A Daza, J F Martin, J A Gil (1990) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 59: 3 259-264<br/>

http://publicationslist.org/j.a.gil/refid37 2010-06-01T18:56:55Z

A high L-asparaginase (L-asparagine amidohydrolase: EC 3.5.1.1) activity was found under conditions of lysine overproduction in cultures of Corynebacterium glutamicum. L-Asparaginase was purified 98-fold by protamine sulphate precipitation. DEAE-Sephacel anion exchange, ammonium sulphate precipitation and Sephacryl S-200 gel filtration. The asparaginase protein was subjected to PAGE under non-dena...<br/><br/>J M Mesas, J A Gil, J F Martín (1990) <i>J Gen Microbiol</i> <i></i> <i></i> 136: 3 515-519<br/>

http://publicationslist.org/j.a.gil/refid35 2007-12-14T17:37:46Z

A 7.2 kb Bg/II restriction fragment, which increases the production of several extracellular enzymes, including alkaline phosphatase, amylase, protease, lipase and beta-galactosidase, was cloned in Streptomyces lividans from the DNA of S. griseus ATCC 10137. This gene (named saf) showed a positive gene dosage effect on production of extracellular enzymes. When the saf gene was introduced into cell...<br/><br/>A Daza, J A Gil, T Vigal, J F Martin (1990) <i>Mol Gen Genet</i> <i></i> <i></i> 222: 2-3 384-392<br/>

http://publicationslist.org/j.a.gil/refid48 2010-06-01T18:55:52Z

Streptomyces griseus ATCC 10137, S. griseus IMRU 3570, S. griseus JI 2212, S. acrimycini JI 2236 and S. albus G sporulated abundantly in several liquid media after nutritional downshift. Spores formed in submerged cultures were viable and as thermoresistant as aerial spores. Scanning electron microscopy showed that submerged spores are morphologically similar to aerial spores. The sporulation of t...<br/><br/>A Daza, J F Martín, A Dominguez, J A Gil (1989) <i>J Gen Microbiol</i> 135: 9 2483-2491<br/>

http://publicationslist.org/j.a.gil/refid40 2007-12-14T17:37:46Z

J F Martin, A Daza, T Vigal, T Alegre, M D Garcia, P Liras, J A Gil (1989) <i>Biochem Soc Trans</i> <i></i> <i></i> 17: 2 342-344<br/>

http://publicationslist.org/j.a.gil/refid39 2007-12-14T17:37:46Z

Phosphate strongly repressed the formation of p-aminobenzoic acid (PABA) synthase, an enzyme involved in candicidin biosynthesis. Expression in Streptomyces lividans of the pabS gene (encoding PABA synthase) of Streptomyces griseus is repressed by phosphate at concentrations above 0.1 mM. However, expression of the pabS gene in Escherichia coli is not regulated by phosphate. Phosphate control of t...<br/><br/>A Rebollo, J A Gil, P Liras, J A Asturias, J F Martín (1989) <i>Gene</i> <i></i> <i></i> 79: 1 47-58<br/>

http://publicationslist.org/j.a.gil/refid38 2007-12-14T17:37:46Z

The nucleotide sequence of a gene (cat) encoding chloramphenicol acetyltransferase (CAT) in Streptomyces acrimycini was determined. The predicted amino acid sequence demonstrates extensive homology with those of CATs isolated from Gram-negative Enterobacteria, notably with the type III variant encoded by the IncK plasmid R387. Transcript mapping indicates a single cat mRNA with a 5' end coinciding...<br/><br/>I A Murray, J A Gil, D A Hopwood, W V Shaw (1989) <i>Gene</i> <i></i> <i></i> 85: 2 283-291<br/>

http://publicationslist.org/j.a.gil/refid52 2007-12-14T20:10:30Z

J.F. Martín, R. Santamaría, H. Sandoval, G. del Real, L.M. Mateos, J.A. Gil and A. Aguilar (1987) <i>Nature Biotechnology</i> 5: 137-146<br/>

http://publicationslist.org/j.a.gil/refid41 2007-12-14T17:37:46Z

A strong promoter P1 has been found in plasmid pUL340, a cloning vector used to transform corynebacteria. This promoter is also expressed efficiently in Escherichia coli. A gene (cat) for chloramphenicol acetyltransferase from Streptomyces acrimycini and a gene (hyg) for hygromycin phosphotransferase from Streptomyces hygroscopicus were subcloned in different positions of the Brevibacterium lactof...<br/><br/>R I Santamaría, J F Martín, J A Gil (1987) <i>Gene</i> <i></i> <i></i> 56: 2-3 199-208<br/>

http://publicationslist.org/j.a.gil/refid44 2007-12-14T17:37:46Z

A gene (cat) for chloramphenicol (Cm) acetyltransferase (CAT) was cloned from Streptomyces acrimycini into S. lividans 66 on the plasmid vector pIJ61. The cat gene was localized on a 1.7-kb BclI fragment, which probably also carries the cat promoter. This DNA fragment conferred Cm resistance, through CAT activity, on S. lividans, S. coelicolor and S. parvulus, but not on Escherichia coli when inse...<br/><br/>J A Gil, H M Kieser, D A Hopwood (1985) <i>Gene</i> <i></i> <i></i> 38: 1-3 1-8<br/>

http://publicationslist.org/j.a.gil/refid43 2010-06-01T19:11:21Z

An efficient polyethylene glycol-assisted method for transformation of Brevibacterium lactofermentum protoplasts that uses plasmid vectors has been developed. Two small plasmids, pUL330 (5.2 kilobases) and pUL340 (5.8 kilobases), both containing the kanamycin resistance gene from transposon Tn5 and the replication origin of the natural plasmid pBL1 of B. lactofermentum, were selected as vectors. S...<br/><br/>R I Santamaria, J A Gil, J F Martin (1985) <i>J Bacteriol</i> <i></i> <i></i> 162: 1 463-467<br/>

http://publicationslist.org/j.a.gil/refid42 2010-06-01T18:51:13Z

p-Aminobenzoic acid synthase (PABA synthase) of Streptomyces griseus catalyses the conversion of chorismic acid to p-aminobenzoic acid (PABA), a precursor of the aromatic p-aminoacetophenone moiety of candicidin, a polyene macrolide antibiotic. This enzyme uses glutamine or ammonia as amino donors for PABA formation. Enzyme extracts converted [14C]chorismic acid to labelled PABA. PABA synthase was...<br/><br/>J A Gil, G Naharro, J R Villanueva, J F Martín (1985) <i>J Gen Microbiol</i> <i></i> <i></i> 131: 6 1279-1287<br/>

http://publicationslist.org/j.a.gil/refid50 2007-12-14T20:07:56Z

J.F. Martín, J.A. Gil (1984) <i>Nature Biotechnology</i> 2: 63-72<br/>

http://publicationslist.org/j.a.gil/refid65 2010-06-01T18:47:41Z

Santamaría RI. Gil JA. Mesas J and Martín JF (1984) <i>Journal of General Microbiology</i> 130: 2237 - 2246<br/>

http://publicationslist.org/j.a.gil/refid51 2007-12-14T20:09:34Z

J.F. Martín, J.A. Gil. (1983) <i>Nature Biotechnology</i> 1: 483‑484<br/>

http://publicationslist.org/j.a.gil/refid49 2007-12-14T20:06:29Z

D.A. Hopwood, M.J. Bibb, C.J. Bruton, K.F. Chater, J.S. Feitelson and J.A. Gil (1983) <i>Trends in Biotechnology</i> 1: 42‑48<br/>

http://publicationslist.org/j.a.gil/refid45 2010-06-02T07:08:53Z

4.5-kb BamHI fragments of DNA coding for p-aminobenzoic acid (PABA) synthetase from the candicidin-producing Streptomyces griseus IMRU 3570 and from a sulphonamide resistant mutant of it were cloned on the plasmid vector pIJ41 into Streptomyces lividans 66. The cloned DNA restored prototrophy to a pab auxotroph of S. lividans; when the S. griseus donor was a sulphonamide resistant, PABA-overproduc...<br/><br/>J A Gil, D A Hopwood (1983) <i>Gene</i> <i></i> <i></i> 25: 1 119-132<br/>

http://publicationslist.org/j.a.gil/refid46 2010-06-01T18:50:04Z

The biosynthesis by Streptomyces griseus of candicidin, an aromatic polyene macrolide antibiotic, was inhibited by L-tryptophan, L-phenylalanine and, to a lesser degree, by L-tyrosine. A mixture of the three aromatic amino acids inhibited candicidin biosynthesis to a greater extent than did each amino acid separately. L-Tryptophan strongly inhibited the incorporation of the labelled precursors pro...<br/><br/>J A Gil, P Liras, G Naharro, J R Villanueva, J F Martín (1980) <i>J Gen Microbiol</i> <i></i> <i></i> 118: 1 189-195<br/>

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J F Martín, J A Gil (1979) <i>Jpn J Antibiot</i> <i></i> <i></i> 32 Suppl: S122-S128<br/>