Jean-Claude Mollet's Publications List

Combined Experimental and computational approaches reveal distinct pH-dependence of pectin methyl esterase inhibitors

2017-08-25T15:06:21Z

The fine-tuning of the degree of methylesterification of cell wall pectin is a key to regulate cell elongation and ultimately the shape of plant body. Pectin methylesterification is spatio-temporally controlled by pectin methylesterases (PMEs, 66 members in Arabidopsis). The comparably large number of proteinaceous pectin methylesterase inhibitors (PMEIs, 76 members in Arabidopsis) questions the s...

Hocq L, Sénéchal F, Lefebvre V, Lehner A, Domon JM, Mollet JC, Dehors J, Pageau K, Marcelo P, Guérineau F, Kolšek K, Mercadante D, Pelloux J (2017) Plant Physiology 173: 1075-1093

Holaphyllamine, a steroid, is able to induce defense responses in Arabidopsis thaliana and increases resistance against bacterial infection

2017-08-25T15:03:11Z

Main conclusion A chemical screen of plant-derived compounds identified holaphyllamine, a steroid, able to trigger defense responses in Arabidopsis thaliana and improve resistance against the pathogenic bacterium Pseudomonas syringae pv tomato DC3000. A chemical screen of 1600 plant-derived compounds was conducted and allowed the identification of a steroid able to activate defense responses in...

Zahid A, Jaber R, Laggoun F, Lehner A, Remy-Jouet I, Leprince J, Follet-Gueye ML, Vicré-Gibouin M, Pamlard O, Beaupierre S, Latour X, Richard V, Guillou C, Lerouge P, Driouich A, Mollet JC (2017) Planta :

UUAT1 is a Golgi-localized UDP-uronic acid transporter that modulates the polysaccharide composition of Arabidopsis seed mucilage

2017-02-10T17:15:45Z

UDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides, and is required for production of seed mucilage. Following synthesis in the cytosol, it is transported into the lumen of the Golgi apparatus, where it is converted to UDP-galacturonic acid (UDP-GalA), UDP-arabinose and UDP-xylose. To identify the Golgi-localized UDP-GlcA transporter, we screened Arabidopsis th...

Saez-Aguayo S, Rautengarten C, Sanhueza D, Temple H, Ejsmentewicz T, Sandoval-Ibañez O, Doñas D, Ebert B, Lehner A, Mollet JC, Dupree P, Scheller HV, Heazlewood JL, Reyes FC, Orellana A (2017) Plant Cell 29: 129-143

AtPME3, a ubiquitous cell wall pectin methylesterase of Arabidopsis thaliana, alters the metabolism of cruciferin seed storage proteins during post-germinative growth of seedlings

2017-04-04T15:28:26Z

AtPME3 (At3g14310) is a ubiquitous cell wall pectin methylesterase. Atpme3-1 loss-of-function mutants exhibited distinct phenotypes from the wild type (WT), and were characterized by earlier germination and reduction of root hair production. These phenotypical traits were correlated with the accumulation of a 21.5-kDa protein in the different organs of 4-day-old Atpme3-1 seedlings grown in the dar...

Guénin S, Hardouin J, Paynel F, Müller K, Mongelard G, Driouich A, Lerouge P, Kermode AR, Lehner A, Mollet JC, Pelloux J, Gutierrez L, Mareck A (2017) Journal of Experimental Botany 68: 1083-1095

Plant cell wall imaging by metabolic click-mediated labelling of rhamnogalacturonan II using azido 3-deoxy-D-manno-oct-2-ulosonic acid analogue

2016-05-09T16:00:44Z

In plants, 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) is a monosaccharide that is only found in the cell wall pectin, rhamnogalacturonan-II (RG-II). Incubation of 4-day-old light-grown Arabidopsis seedlings or tobacco BY-2 cells with 8-azido 8-deoxy Kdo (Kdo-N3) followed by coupling to an alkyne-containing fluorescent probe resulted in the specific in muro labelling of RG-II through a copper-cat...

Dumont M, Lehner A, Vauzeilles B, Malassis J, Marchant A, Smyth K, Linclau B, Baron A, Mas Pons J, Anderson CT, Mollet JC, Lerouge P (2016) The Plant Journal 25: 437-447

Salicylic acid regulates pollen tip growth through a NPR3/NPR4-independent pathway

2016-11-21T12:32:41Z

Tip growth is a common strategy for the rapid elongation of cells to forage the environment and/or to target to long-distance destinations. In the model tip growth system, Arabidopsis pollen tubes, several small molecule hormones regulate their elongation, but how these rapidly-diffusing molecules control extremely localized growth remains mysterious. Here we show that the interconvertible salicyl...

Rong D, Luo N, Mollet JC, Liu X, Yang Z (2016) Molecular Plant 9: 1478-1491

Two tomato GDP-D-mannose epimerase isoforms involve in ascorbate biosynthesis play specific roles in cell wall biosynthesis and development.

2016-08-29T12:31:01Z

GDP-D-mannose epimerase (GME, EC 5.1.3.18) converts GDP-D-mannose to GDP-L-galactose, and is considered to be a central enzyme connecting the major ascorbate biosynthesis pathway to primary cell wall metabolism in higher plants. Our previous work demonstrated that GME is crucial for both ascorbate and cell wall biosynthesis in tomato. The aim of the present study was to investigate the respective ...

Louise Mounet-Gilbert, Marie Dumont, Carine Ferrand, Céline Bournonville, Antoine Monier, Joana Jorly, Martine Lemaire-Chamley, Kentaro Mori, Isabelle Atienza, Michel Hernould, Rebecca Stevens, Arnaud Lehner, Jean Claude Mollet, Christophe Rothan, Patrice Lerouge, Pierre Baldet (2016) Journal of experimental botany 67: 4767-4777

PECTIN METHYLESTERASE48 is involved in Arabidopsis pollen grain germination

2015-12-22T11:03:27Z

Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis thaliana pollen. A combination of functional genomics, gene expression, in vivo and in vitro pollen germination, immunolabeling and biochemical a...

Christelle Leroux, Sophie Bouton, Marie-Christine Kiefer-Meyer, Tohnyui Ndinyanka Fabrice, Alain Mareck, Stéphanie Guénin, Françoise Fournet, Christoph Ringli, Jérôme Pelloux, Azeddine Driouich, Patrice Lerouge, Arnaud Lehner, Jean-Claude Mollet (2015) Plant Physiology 167: 367–380

A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers

2015-12-22T10:46:08Z

Dumont M, Cataye C, Lehner A, Maréchal E, Lerouge P, Falconet D, Mollet JC (2015) 5

Pollen tube cell walls of wild and domesticated tomatoes contain arabinosylated and fucosylated xyloglucan

2014-12-20T17:31:46Z

Background and Aims In flowering plants, fertilization relies on the delivery of the sperm cells carried by the pollen tube to the ovule. During the tip growth of the pollen tube, proper assembly of the cell wall polymers is required to maintain the mechanical properties of the cell wall. Xyloglucan (XyG) is a cell wall polymer known for maintaining the wall integrity and thus allowing cell expa...

Flavien Dardelle, François Le Mauff, Arnaud Lehner, Corinne Loutelier-Bourhis, Muriel Bardor, Christophe Rihouey, Mathilde Causse, Patrice Lerouge, Azeddine Driouich, Jean-Claude Mollet (2015) Annals of Botany 115: 55-66

In silico prediction of proteins related to xyloglucan fucosyltransferases in Solanaceae genomes

2015-12-22T10:52:08Z

Lehner A, Menu-Bouaouiche L, Dardelle F, Le Mauff F, Driouich A, Lerouge P, Mollet JC (2015) Plant Signaling & Behavior 10: 6 e1026023

Analysis of Sugar Component of a Hot Water Extract from Arabidopsis thaliana Pollen Tubes Using GC-EI-MS

2015-12-22T10:48:11Z

Dumont M, Lehner A, Loutelier-Bourhis C, Mollet JC, Lerouge P (2015) 5

Modifications of cell wall pectin in tomato cell suspension in response to cadmium and zinc

2015-12-22T10:53:59Z

Muschitz A, Riou C, Mollet J-C, Gloaguen V, Faugeron C (2015) Acta Physiologiae Plantarum 37: 245

Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy-L-fucose induces defects in root cell elongation

2016-05-09T15:51:24Z

Screening of commercially available fluoro monosaccharides as putative growth inhibitors in Arabidopsis thaliana revealed that 2-fluoro 2-L-fucose (2F-Fuc) reduces root growth at micromolar concentrations. The inability of 2F-Fuc to affect an Atfkgp mutant that is defective in the fucose salvage pathway indicates that 2F-Fuc must be converted to its cognate GDP nucleotide sugar in order to inhi...

Dumont M, Lehner A, Bardor M, Burel C, Vauzeilles B, Lerouxel O, Anderson CT, Mollet J-C, Lerouge P (2015) The Plant Journal 84: 1137-1151

The cell wall pectic polymer rhamnogalacturonan-II is required for proper pollen tube elongation: implication of a putative sialyltransferase-like protein.

2014-12-20T14:24:49Z

Background and Aims Rhamnogalacturonan-II (RG-II) is one of the pectin motifs found in the cell wall of all land plants. It contains sugars such as 2-keto-3-deoxy-d-lyxo-heptulosaric acid (Dha) and 2-keto-3-deoxy-d-manno-octulosonic acid (Kdo), and within the wall RG-II is mostly found as a dimer via a borate diester cross-link. To date, little is known regarding the biosynthesis of this motif...

Dumont M, Lehner A, Bouton S, Kiefer-Meyer MC, Voxeur A, Pelloux J, Lerouge P, Mollet JC (2014) Annals of Botany 114: 1177-1188.

Kiwi fruit PMEI inhibits PME activity, modulates root elongation and induces pollen tube burst in Arabidopsis thaliana

2014-12-20T14:25:38Z

Pectins are major components of primary cell wall that play a crucial role in plant development. After biosynthesis, pectins are secreted in the cell wall by Golgi-derived vesicles under a highly methylesterified form and are de-methylesterified by pectin methylesterases (PME). It is hypothesized that PME might be regulated by pectin methylesterase inhibitor (PMEI). In this paper, we show by isoel...

Paynel F, Leroux C, Surcouf O, Schaumann A, Pelloux J, Driouich A, Mollet JC, Lerouge P, Lehner A, Mareck A (2014) Plant Growth Regulation 74: 285-297

Effect of water deficit on the cell wall of the date palm (Phoenix dactylifera ‘Deglet nour’, Arecales) fruit during development

2014-08-30T13:00:43Z

Date palm (Phoenix dactylifera) is an important crop providing a valuable nutrition source for people in many countries including the Middle East and North Africa. In recent years, the amount of rain in North Africa and especially in the Tunisian palm grove areas has dropped significantly. We investigated the growth and cell wall remodelling of fruits harvested at three key development stages from...

Gribaa A, Dardelle F, Lehner A, Rihouey C, Burel C, Ferchichi A, Driouich A, Mollet JC. (2013) Plant, Cell and Environment 36: 1056-1070

Cell Wall Composition, Biosynthesis and Remodeling during Pollen Tube Growth

2013-03-08T11:45:55Z

The pollen tube is a fast tip-growing cell carrying the two sperm cells to the ovule allowing the double fertilization process and seed setting. To succeed in this process, the spatial and temporal controls of pollen tube growth within the female organ are critical. It requires a massive cell wall deposition to promote fast pollen tube elongation and a tight control of the cell wall remodeling to ...

Jean-Claude Mollet , Christelle Leroux, Flavien Dardelle, Arnaud Lehner (2013) Plants 2: 107-147

Immuno-Glyco-imaging in plant cells: localization of cell wall carbohydrate epitopes and their biosynthesizing enzymes

2012-03-09T11:31:10Z

Follet-Gueye ML, Mollet JC, Vicré-Gibouin M, Bernard S, Chevalier L, Plancot B, Dardelle F, Ramdani Y, Coimbra S, Driouich A (2012) Applications of Immunocytochemistry : 297-320

Arabinogalactan-proteins in root and pollen tube cells: distribution and functional aspects

2012-07-19T09:26:05Z

Background Arabinogalactan proteins (AGPs) are complex proteoglycans of the cell wall found in the entire plant kingdom and in almost all plant organs. AGPs encompass a large group of heavily glycosylated cell-wall proteins which share common features, including the presence of glycan chains especially enriched in arabinose and galactose and a protein backbone particularly rich in hydroxyproli...

Nguema-Ona E, Coimbra S, Vicré-Gibouin M, Mollet JC, Driouich A (2012) Annals of Botany 110: 383-404

Pectins in the cell wall of Arabidopsis thaliana pollen tube and pistil

2011-03-31T10:54:30Z

Plant sexual reproduction involves the growth of tip-polarized pollen tubes through the female tissues in order to deliver the sperm nuclei to the egg cells. Despite the importance of this crucial step, little is known about the molecular mechanisms involved in this spatial and temporal control of the tube growth. In order to study this process and to characterize the structural composition of the...

Lehner A, Dardelle F, Soret-Morvan O, Lerouge P, Driouich A, Mollet JC (2010) Plant Signaling and Behavior 5: 1282-1285

Biochemical and immunocytological characterizations of Arabidopsis thaliana pollen tube cell wall

2011-03-30T15:59:17Z

During plant sexual reproduction, pollen germination and tube growth require development under tight spatial and temporal control for the proper delivery of the sperm cells to the ovules. Pollen tubes are fast growing tip-polarized cells able to perceive multiple guiding signals emitted by the female organ. Adhesion of pollen tubes via cell wall molecules may be part of the battery of signals. In ...

Dardelle F, Lehner A, Ramdani Y, Bardor M, Lerouge P, Driouich A, Mollet JC (2010) Plant Physiology 153: 1563-1576

Isolation, characterization and valorization of hemicelluloses from Aristida pungens leaves as biomaterial

2011-03-30T15:56:27Z

Aristida pungens, a perennial grass widely distributed on the Algerian arid regions, may potentially be the source of raw cell wall material to promote a local development in North Africa. Hemicelluloses, the main matrix cell wall polymers of this species were isolated from leaves with 14% KOH aqueous solution, characterized and converted as plastic material. The extraction procedure yielded two h...

Chaa L, Joly N, Lequart V, Faugeron C, JP Cavrot, Mollet J-C, Martin P, Morvan H (2008) Carbohydrate Polymers 74: 597-602

A relationship between structural features and lily pollen tube adhesion activity of SCA (Stigma/style Cysteine-rich Adhesin) isoforms

2011-03-31T12:52:12Z

Lily pollen tubes grow adhering to an extracellular matrix produced by the transmitting tract epidermis in a hollow style. SCA, a small (∼9.4 kDa), basic protein plus low esterified pectin from this extracellular matrix are involved in the pollen tube adhesion event. The mode of action for this adhesion event is unknown. We partially separated three SCA isoforms from the lily stigma in serial si...

Chae K, Zhang K, Zhang L, Morikis D, Kim S, Mollet J-C, de LaRosa N, Tan K, Lord EM (2007) Journal of Biological Chemistry 282: 33845-33858

New fractionation method for kappa- and lambda-carrageenans isolated from Gigartina pistillata (Rhodophyta).

2011-03-31T11:51:04Z

Carrageenans isolated from the cell wall of Gigartina pistillata (Gmelin) Stackhouse were investigated using two extraction methods. The first one involved sequential extraction with increasing temperatures starting from 20°C up to 100°C by 20°C increment. The second method was carried out by using successive ethanol concentrations ranging from 80, 60, 40, 20 and 0%. Chemical characterization o...

Mouradi A, Amimi A, Mollet J-C, Givernaud T (2007) Algological Studies 122: 107-122

Cell adhesion, separation and guidance in compatible plant reproduction

2012-03-09T11:36:36Z

Mollet J-C, Faugeron C, Morvan H (2007) Plant Cell separation and adhesion 69-90

Activities of de-N-glycosylation are ubiquitously found in tomato plant

2011-03-31T11:49:04Z

Activities of two de-N-glycosylation enzymes, PNGase (peptide N 4(N-acetyl-glucosaminyl)asparagine amidase) and ENGase (endo N-acetyl-β-D-glucosaminidase), involved in the release of N-glycans from N-glycoproteins, were monitored in several organs of tomato plants (Lycopersicon esculentum, Mill., cv. Dombito) with a fluorescence-HPLC procedure using a resofurin-labelled N-glycopeptide substrate....

Faugeron C, Mollet J-C, Karamanos Y, Morvan H (2006) Acta Physiologiae Plantarum 28: 417-425

Lily (Lilium longiflorum L.) pollen protoplast adhesion is increased in the presence of the peptide SCA

2011-03-31T10:06:07Z

The style of lily produces a specialized extracellular matrix (ECM) in the transmitting tract epidermis that functions to guide pollen tubes to the ovary. This adhesive ECM contains low esterified pectins and a peptide, SCA (stigma/stylar cysteine-rich adhesin). Together they form a matrix to which pollen tubes adhere as they grow through the style. Pollen tubes also adhere to each other but only ...

Zhao J, Mollet J-C, Lord EM (2004) Sexual Plant Reproduction 16: 227-233

In vivo pollen tube growth: tube cell adhesion and movement in lily

2012-03-09T11:36:11Z

Tip growth is a type of cellular growth that is represented in many and diverse cell types, which are important to plant breeding (pollen tubes), agriculture (root hairs) and plant and animal pathology (fungal hyphae). Also moss and fern protonemata as well as algal zygotes and yeasts exhibit the features unique for tip growth: directable apical linear growth which renders the cell able to penetra...

Lord EM, Mollet J-C, Park S-Y (2003) Cell Biology of Plant and Fungal Tip Growth 187-201

Interaction of a stylar pectic polysaccharide and a basic protein (SCA) mediates lily pollen tube adhesion

2012-03-09T11:32:31Z

Mollet J-C, Park S-Y, Lord EM (2003) Advances in Pectin and Pectinase Research 1-13

Chemocyanin, a small basic protein from the lily stigma, induces pollen tube chemotropism

2011-03-31T10:14:37Z

In plant reproduction, pollination is an essential process that delivers the sperm through specialized extracellular matrices (ECM) of the pistil to the ovule. Although specific mechanisms of guidance for pollen tubes through the pistil are not known, the female tissues play a critical role in this event. Many studies have documented the existence of diffusible chemotropic factors in the lily stig...

Kim S, Mollet J-C, Dong J, Zhang K, Park S-Y, Lord EM (2003) Proceedings of the National Academy of Sciences USA 100: 16125-16130

AGPs, pollen tube growth and the reversible effects of Yariv phenylglycoside

2011-03-31T10:22:55Z

Arabinogalactan proteins (AGPs) are abundant complex macromolecules involved in both reproductive and vegetative plant growth. They are secreted at pollen tube tips in Lilium longiflorum. Here, we report the effect of the (β-D-glucosyl)3 Yariv phenylglycoside, known to interact with AGPs, on pollen tube extension in several plant species. In Annona cherimola the Yariv reagent clearly inhibited po...

Mollet J-C, Kim S, Jauh, G-Y, Lord EM (2002) Protoplasma 219: 89-98

Plant cell adhesion: a bioassay facilitates discovery of the first pectin biosynthetic gene

2011-03-31T10:18:55Z

Lord EM, Mollet J-C (2002) Proceedings of the National Academy of Sciences USA 99: 15843-15845

A lipid transfer-like protein is necessary for lily pollen tube adhesion to an in vitro stylar matrix

2011-03-31T10:29:26Z

Flowering plants possess specialized extracellular matrices in the female organs of the flower that support pollen tube growth and sperm cell transfer along the transmitting tract of the gynoecium. Transport of the pollen tube cell and the sperm cells involves a cell adhesion and migration event in species such as lily that possess a transmitting tract epidermis in the stigma, style, and ovary. A ...

Park S-Y, Jauh G-Y, Mollet J-C, Eckard KJ, Nothnagel EA, Walling LL, Lord EM (2000) The Plant Cell 12: 151-163

A lily stylar pectin is necessary for pollen tube adhesion to an in vitro stylar matrix

2011-03-31T10:26:41Z

Pollen tube cells adhere to the wall surface of the stylar transmitting tract epidermis in lily. This adhesion has been proposed as essential for the proper delivery of the sperm cells to the ovule. An in vitro adhesion bioassay has been used to isolate two stylar molecules required for lily pollen tube adhesion. The first molecule was determined to be a small, cysteine-rich protein with some sequ...

Mollet J-C, Park S-Y, Nothnagel EA, Lord EM (2000) The Plant Cell 12: 1737-1749

AGPs, pollen tube growth and effect of Yariv phenylglycoside

2012-03-09T11:35:05Z

Mollet J-C, Kim S, Jauh G-Y, Lord EM (2000) Cell and Developmental Biology of Arabinogalactan-Proteins 284-285

Yield, chemical composition and gel strength of agarocolloids of Gracilaria gracilis, Gracilariopsis longissima and the newly reported Gracilaria cf. vermiculophylla from Roscoff (Brittany).

2011-03-31T10:32:19Z

Agarocolloids were extracted from field samples of Gracilaria gracilis, Gracilariopsis longissima and the newly reported Gracilaria cf. vermiculophylla harvested at different periods of the year near Roscoff (France). Native and alkali modified extracts were characterized by GLC, HPLC and FT-IR spectroscopy. The main components of agarocolloids isolated by freeze-thawing method, were 3,6-anhydroga...

Mollet J-C, Rahaoui A, Lemoine Y (1998) Journal of Applied Phycology 10: 59-66

Analysis of red algal extracellular matrix polysaccharides: Cellulose and Carrageenan

2012-03-09T11:34:33Z

Gretz MR, Mollet J-C, Falshaw R (1997) Techniques in Glycobiology 613-628

Extracellular matrix assembly in diatoms (Bacillariophyceae). II. 2,6-dichloro-benzonitrile inhibition of motility and stalk production in Achnanthes longipes.

2011-03-31T10:52:28Z

The cellulose synthesis inhibitor 2,6-dichlorobenzonitrile (DCB) and the DCB analogs 2-chloro-6-fluorobenzonitrile, 3-amino-2,6-dichlorobenzonitrile, and 5-dimethylamino-naphthalene-1-sulfonyl-(3-cyano-2, 4-dichloro)aniline (DCBF) inhibited extracellular adhesive production in the marine diatom Achnanthes longipes, resulting in a loss of motility and a lack of permanent adhesion. The effect was fu...

Wang Y, Lu J, Mollet J-C, Gretz MR, Hoagland KD (1997) Plant Physiology 113: 1071-1080

Improved protoplast yield and cell wall regeneration in Gracilaria verrucosa (Huds.) Papenfuss (Gracilariales, Rhodophyta).

2011-03-31T12:21:55Z

Protoplasts were isolated enzymatically from the red alga Gracilaria verrucosa using only two enzymes: agarase prepared from marine bacteria and commercial cellulase. Yields of protoplasts were dependent on the donor material and by choosing young blades or algae in a state of higher growth rate, the production of protoplasts reached a maximum of 107 protoplasts per gram of fresh tissue. Cell viab...

Mollet J-C, Verdus M-C, Kling R, Morvan H (1995) Journal of Experimental Botany 46: 239-247

Direct structural identification of polysaccharides from red algae by FTIR microspectrometry. I. Localization of agar in Gracilaria verrucosa sections

2011-03-31T12:26:35Z

Unlike carrageenans, agars have not been studied very extensively by infrared spectroscopy, in so far as the structures of this kind of polygalactanes are not as well defined as carrageenans. However, in a previous work we have carried out a vibrational analysis of both carrageenans and agars and some important assignments of the main absorptions have been made. Consequently, the present work has ...

Sekkal M, Huvenne J-P, Legrand P, Sombret B, Mollet J-C, Mouradi-Givernaud A, Verdus M-C (1993) Microchimica Acta 112: 1-10