http://publicationslist.org/data/joaquin.arino/atom.xml Joaquin Arinohttp://publicationslist.org/joaquin.arino$basepathfavicon.icoRecent additions to Joaquin Arino's PublicationsList.org pagehttp://publicationslist.org/publications.png2008-04-19T08:52:20Z http://publicationslist.org/joaquin.arino/refid4 2008-04-19T08:48:20Z

The TRP1 marker has been commonly used for gene disruption experiments and subsequent phenotypic analysis. However, introduction of the TRP1 gene into a trp1 strain markedly affects growth under many conditions used for phenotypic profiling. Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses.<br/><br/>Asier González, Carolina Larroy, Josep A Biosca, Joaquín Ariño (2008) <i>FEMS Yeast Res</i> <i></i> <i></i> 8: 1 2-5<br/>

http://publicationslist.org/joaquin.arino/refid2 2008-04-19T08:48:20Z

Failure to use glucose as carbon source results in transcriptional activation of numerous genes whose expression is otherwise repressed. HXT2 encodes a yeast high-affinity glucose transporter that is only expressed under conditions of glucose limitation. We show that HXT2 is rapidly and potently induced by environmental alkalinization and this requires both the Snf1 and the calcineurin pathways. R...<br/><br/> Ruiz, Serrano, Arino (2008) <i>J Biol Chem</i> <i></i> <i></i> : <br/>

http://publicationslist.org/joaquin.arino/refid1 2008-04-19T08:48:20Z

Protein kinase CK2 is an evolutionary conserved Ser/Thr phosphotransferase composed by two distinct subunits, alpha (catalytic), and beta (regulatory), which combine to form a tetrameric complex. Plant genomes contain multiple genes for each subunit, the expression of which gives rise to different active holoenzymes. In order to study the effects of loss-of-function of CK2 on plant development we ...<br/><br/> Moreno-Romero, Espunya, Platara, Ariño, Martínez (2008) <i>Plant J</i> <i></i> <i></i> : <br/>

http://publicationslist.org/joaquin.arino/refid8 2008-04-19T08:48:20Z

Regulatable gene expression is a powerful genetic tool for analyzing the function of a given gene product. The use of tetracycline-regulatable promoters in yeast represents a substantial improvement over previously described methods for gene regulation. Here we show how this approach can be used to analyze the biological role of serine/threonine phosphatase catalytic or putative regulatory subunit...<br/><br/>Joaquín Ariño, Antonio Casamayor, Amparo Ruiz, Ivan Muñoz, Maribel Marquina (2007) <i>Methods Mol Biol</i> <i></i> <i></i> 365: 299-307<br/>

http://publicationslist.org/joaquin.arino/refid9 2008-04-19T08:48:20Z

We have recently characterized Ypi1 as an inhibitory subunit of yeast Glc7 PP1 protein phosphatase. In this work we demonstrate that Ypi1 forms a complex with Glc7 and Sds22, another Glc7 regulatory subunit that targets the phosphatase to substrates involved in cell cycle control. Interestingly, the combination of equimolar amounts of Ypi1 and Sds22 leads to an almost full inhibition of Glc7 activ...<br/><br/>Leda Pedelini, Maribel Marquina, Joaquin Ariño, Antonio Casamayor, Libia Sanz, Mathieu Bollen, Pascual Sanz, Maria Adelaida Garcia-Gimeno (2007) <i>J Biol Chem</i> <i></i> <i></i> 282: 5 3282-3292<br/>

http://publicationslist.org/joaquin.arino/refid7 2008-04-19T08:48:20Z

The efficiency of stop codons read-through in yeast is controlled by multiple interactions of genetic and epigenetic factors. In this study, we demonstrate the participation of the Hal3-Ppz1 protein complex in regulation of read-through efficiency and manifestation of non-Mendelian anti-suppressor determinant [ISP(+)]. Over-expression of HAL3 in [ISP(+)] strain causes nonsense suppression, whereas...<br/><br/>Anna Aksenova, Iván Muñoz, Kirill Volkov, Joaquín Ariño, Ludmila Mironova (2007) <i>Genes Cells</i> <i></i> <i></i> 12: 4 435-445<br/>

http://publicationslist.org/joaquin.arino/refid5 2008-04-19T08:48:20Z

Mauricio Menacho-Marquez, Jorge Perez-Valle, Joaquin Ariño, Jose Gadea, Jóse R Murguía (2007) <i>Cell Cycle</i> <i></i> <i></i> 6: 18 2302-2305<br/>

http://publicationslist.org/joaquin.arino/refid6 2008-04-19T08:48:20Z

Zinc is an essential metal that, when in excess, can be deleterious to the cell. Therefore, homeostatic mechanisms for this cation must be finely tuned. To better understand the response of yeast in front of an excess of zinc, we screened a systematic deletion mutant library for altered growth in the presence of 6 mM zinc. Eighty-nine mutants exhibited increased zinc sensitivity, including many ge...<br/><br/>M Ayelen Pagani, Antonio Casamayor, Raquel Serrano, Sílvia Atrian, Joaquín Ariño (2007) <i>Mol Microbiol</i> <i></i> <i></i> 65: 2 521-537<br/>

http://publicationslist.org/joaquin.arino/refid3 2008-04-19T08:48:20Z

Amparo Ruiz, Joaquín Ariño (2007) <i>Eukaryot Cell</i> <i></i> <i></i> 6: 12 2175-2183<br/>

http://publicationslist.org/joaquin.arino/refid13 2008-04-19T08:48:20Z

Protein phosphatases 2C are a family of conserved enzymes involved in many aspects of the cell biology. We reported that, in the yeast Saccharomyces cerevisiae, overexpression of the Ptc3p isoform resulted in increased lithium tolerance in the hypersensitive hal3 background. We have found that the tolerance induced by PTC3 overexpression is also observed in wild-type cells and that this is most pr...<br/><br/>Amparo Ruiz, Asier González, Raúl García-Salcedo, José Ramos, Joaquín Ariño (2006) <i>Mol Microbiol</i> <i></i> <i></i> 62: 1 263-277<br/>

http://publicationslist.org/joaquin.arino/refid14 2008-04-19T08:48:20Z

The yeast Debaryomyces hansenii has a remarkable capacity to proliferate in salty and alkaline environments such as seawater. A screen for D. hansenii genes able to confer increased tolerance to high pH when overexpressed in Saccharomyces cerevisiae yielded a single gene, named here DhGZF3, encoding a putative negative GATA transcription factor related to S. cerevisiae Dal80 and Gzf3. Overexpressi...<br/><br/>Raúl García-Salcedo, Antonio Casamayor, Amparo Ruiz, Asier González, Catarina Prista, Maria C Loureiro-Dias, José Ramos, Joaquín Ariño (2006) <i>Eukaryot Cell</i> <i></i> <i></i> 5: 8 1388-1398<br/>

http://publicationslist.org/joaquin.arino/refid12 2008-04-19T08:48:20Z

Adaptive response of the yeast Saccharomyces cerevisiae to environmental alkalinization results in remodeling of gene expression. A key target is the gene ENA1, encoding a Na(+)-ATPase, whose induction by alkaline pH has been shown to involve calcineurin and the Rim101/Nrg1 pathway. Previous functional analysis of the ENA1 promoter revealed a calcineurin-independent pH responsive region (ARR2, 83 ...<br/><br/>Maria Platara, Amparo Ruiz, Raquel Serrano, Aarón Palomino, Fernando Moreno, Joaquín Ariño (2006) <i>J Biol Chem</i> <i></i> <i></i> 281: 48 36632-36642<br/>

http://publicationslist.org/joaquin.arino/refid11 2008-04-19T08:48:20Z

Type 2C protein phosphatases are encoded in Saccharomyces cerevisiae by several related genes (PTC1-5 and PTC7). To gain insight into the functions attributable to specific members of this gene family, we have investigated the transcriptional profiles of ptc1-5 mutants. Two main patterns were obtained as follows: the one generated by the ptc1 mutation and the one resulting from the lack of Ptc2-5....<br/><br/>Asier González, Amparo Ruiz, Raquel Serrano, Joaquín Ariño, Antonio Casamayor (2006) <i>J Biol Chem</i> <i></i> <i></i> 281: 46 35057-35069<br/>

http://publicationslist.org/joaquin.arino/refid10 2008-04-19T08:48:20Z

Alkalinization of the external environment represents a stress situation for Saccharomyces cerevisiae. Adaptation to this circumstance involves the activation of diverse response mechanisms, the components of which are still largely unknown. We show here that mutation of members of the cell integrity Pkc1/Slt2 MAPK module, as well as upstream and downstream elements of the system, confers sensitiv...<br/><br/>Raquel Serrano, Humberto Martín, Antonio Casamayor, Joaquín Ariño (2006) <i>J Biol Chem</i> <i></i> <i></i> 281: 52 39785-39795<br/>

http://publicationslist.org/joaquin.arino/refid19 2008-04-19T08:48:20Z

The yeast gene VHS3 (YOR054c) has been recently identified as a multicopy suppressor of the G(1)/S cell cycle blockade of a conditional sit4 and hal3 mutant. Vhs3 is structurally related to Hal3, a negative regulatory subunit of the Ser/Thr protein phosphatase Ppz1 important for cell integrity, salt tolerance, and cell cycle control. Phenotypic analyses using vhs3 mutants and overexpressing strain...<br/><br/>Amparo Ruiz, Ivan Muñoz, Raquel Serrano, Asier González, Ernesto Simón, Joaquín Ariño (2004) <i>J Biol Chem</i> <i></i> <i></i> 279: 33 34421-34430<br/>

http://publicationslist.org/joaquin.arino/refid20 2008-04-19T08:48:20Z

Exposure of the yeast Saccharomyces cerevisiae to an alkaline environment represents a stress situation that negatively affects growth and results in an adaptive transcriptional response. We screened a collection of 4825 haploid deletion mutants for their ability to grow at mild alkaline pH, and we identified 118 genes, involved in numerous cellular functions, whose absence results in reduced grow...<br/><br/>Raquel Serrano, Dolores Bernal, Ernesto Simón, Joaquín Ariño (2004) <i>J Biol Chem</i> <i></i> <i></i> 279: 19 19698-19704<br/>

http://publicationslist.org/joaquin.arino/refid18 2008-04-19T08:48:20Z

Saccharomyces cerevisiae Hal3 is a conserved protein that binds the carboxyl-terminal catalytic domain of the PP1c (protein phosphatase 1)-related phosphatase Ppz1 and potently inhibits its activity, thus modulating all of the characterized functions so far of the phosphatase. It is unknown how Hal3 binds to Ppz1 and inhibits its activity. Although it contains a putative protein phosphatase 1c bin...<br/><br/>Iván Muñoz, Amparo Ruiz, Maribel Marquina, Anna Barceló, Armando Albert, Joaquín Ariño (2004) <i>J Biol Chem</i> <i></i> <i></i> 279: 41 42619-42627<br/>

http://publicationslist.org/joaquin.arino/refid17 2008-04-19T08:48:20Z

The yeast Saccharomyces cerevisiae has been widely used for the implementation of DNA chip technologies. For this reason and due to the extensive use of this organism for basic and applied studies, yeast DNA chips are being used by many laboratories for expression or genomic analyses. While membrane arrays (macroarrays) offer several advantages, for many laboratories they are not affordable. Here ...<br/><br/>Trinidad M Alberola, José García-Martínez, Oreto Antúnez, Laia Viladevall, Anna Barceló, Joaquín Ariño, José E Pérez-Ortín (2004) <i>Int Microbiol</i> <i></i> <i></i> 7: 3 199-206<br/>

http://publicationslist.org/joaquin.arino/refid16 2008-04-19T08:48:20Z

Exposure of the yeast Saccharomyces cerevisiae to alkaline stress resulted in adaptive changes that involved remodeling the gene expression. Recent evidence suggested that the calcium-activated protein phosphatase calcineurin could play a role in alkaline stress signaling. By using an aequorin luminescence reporter, we showed that alkaline stress resulted in a sharp and transient rise in cytoplasm...<br/><br/>Laia Viladevall, Raquel Serrano, Amparo Ruiz, Gema Domenech, Jesús Giraldo, Anna Barceló, Joaquín Ariño (2004) <i>J Biol Chem</i> <i></i> <i></i> 279: 42 43614-43624<br/>

http://publicationslist.org/joaquin.arino/refid15 2008-04-19T08:48:20Z

The Ppz protein phosphatases have been recently shown to negatively regulate the major potassium transport system in the yeast Saccharomyces cerevisiae, encoded by the TRK1 and TRK2 genes. We have found that, in the absence of the Trk system, Ppz mutants require abnormally high concentrations of potassium to proliferate. This can be explained by the observation that trk1 trk2 ppz1 or trk1 trk2 ppz...<br/><br/>Amparo Ruiz, María del Carmen Ruiz, Miguel Angel Sánchez-Garrido, Joaquín Ariño, José Ramos (2004) <i>FEBS Lett</i> <i></i> <i></i> 578: 1-2 58-62<br/>

http://publicationslist.org/joaquin.arino/refid24 2008-04-19T08:48:20Z

The yeast Nha1 Na(+),K(+)/H(+) antiporter may play an important role in regulation of cell cycle, as high-copy expression of the NHA1 gene is able to rescue the blockage at the G(1)/S transition of cells lacking Sit4 protein phosphatase and Hal3 activities. Interestingly, this function was independent of the role of the antiporter in improving tolerance to sodium cations, it required the integrity...<br/><br/>Ernesto Simón, Anna Barceló, Joaquín Ariño (2003) <i>FEBS Lett</i> <i></i> <i></i> 545: 2-3 239-245<br/>

http://publicationslist.org/joaquin.arino/refid25 2008-04-19T08:48:20Z

Inactivation of HAL3 in the absence of SIT4 function leads to cell cycle arrest at the G(1)-S transition. To identify genes potentially involved in the control of this phase of the cell cycle, a screening for multicopy suppressors of a conditional sit4 hal3 mutant (strain JC002) has been developed. The screening yielded several genes known to perform key roles in cell cycle events, such as CLN3, B...<br/><br/>Ivan Muñoz, Ernesto Simón, Núria Casals, Josep Clotet, Joaquín Ariño (2003) <i>Yeast</i> <i></i> <i></i> 20: 2 157-169<br/>

http://publicationslist.org/joaquin.arino/refid23 2008-04-19T08:48:20Z

The Saccharomyces cerevisiae open reading frame YFR003c encodes a small (155-amino acid) hydrophilic protein that we identified as a novel, heat-stable inhibitor of type 1 protein phosphatase (Ypi1). Ypi1 interacts physically in vitro with both Glc7 and Ppz1 phosphatase catalytic subunits, as shown by pull-down assays. Ypi1 inhibits Glc7 but appears to be less effective toward Ppz1 phosphatase act...<br/><br/>Maria Adelaida García-Gimeno, Iván Muñoz, Joaquín Ariño, Pascual Sanz (2003) <i>J Biol Chem</i> <i></i> <i></i> 278: 48 47744-47752<br/>

http://publicationslist.org/joaquin.arino/refid21 2008-04-19T08:48:20Z

Joaquín Ariño, Enric Herrero (2003) <i>Methods Enzymol</i> <i></i> <i></i> 366: 347-358<br/>

http://publicationslist.org/joaquin.arino/refid22 2008-04-19T08:48:20Z

Saccharomyces cerevisiae strains lacking the Ppz1 protein phosphatase are salt tolerant and display increased expression of the ENA1 Na(+)-ATPase gene, a major determinant for sodium extrusion, while cells devoid of the similar Ppz2 protein do not show these phenotypes. However, a ppz1 ppz2 mutant displays higher levels of ENA1 expression than the ppz1 strain. We show here that the increased activ...<br/><br/>Amparo Ruiz, Lynne Yenush, Joaquín Ariño (2003) <i>Eukaryot Cell</i> <i></i> <i></i> 2: 5 937-948<br/>

http://publicationslist.org/joaquin.arino/refid30 2008-04-19T08:48:20Z

During the last decade several novel yeast genes encoding proteins related to the PPP family of Ser/Thr protein phosphatases have been discovered and their functional characterization initiated. Most of these novel phosphatases display intriguing structural features and/or are involved in a number of important functions, such as cell cycle regulation, protein synthesis and maintenance of cellular ...<br/><br/>Joaquín Ariño (2002) <i>Eur J Biochem</i> <i></i> <i></i> 269: 4 1072-1077<br/>

http://publicationslist.org/joaquin.arino/refid31 2008-04-19T08:48:20Z

Carnitine octanoyltransferase (COT) and carnitine palmitoyltransferase (CPT) I, which facilitate the transport of medium- and long-chain fatty acids through the peroxisomal and mitochondrial membranes, are physiologically inhibited by malonyl-CoA. Using an "in silico" macromolecular docking approach, we built a model in which malonyl-CoA could be attached near the catalytic core. This disrupts the...<br/><br/>Montserrat Morillas, Paulino Gómez-Puertas, Blanca Rubí, Josep Clotet, Joaquín Ariño, Alfonso Valencia, Fausto G Hegardt, Dolors Serra, Guillermina Asins (2002) <i>J Biol Chem</i> <i></i> <i></i> 277: 13 11473-11480<br/>

http://publicationslist.org/joaquin.arino/refid29 2008-04-19T08:48:20Z

The yeast Ppz protein phosphatases and the Hal3p inhibitory subunit are important determinants of salt tolerance, cell wall integrity and cell cycle progression. We present several lines of evidence showing that these disparate phenotypes are connected by the fact that Ppz regulates K+ transport. First, salt tolerance, cell wall integrity and cell cycle phenotypes of Ppz mutants are dependent on t...<br/><br/>Lynne Yenush, José M Mulet, Joaquín Ariño, Ramón Serrano (2002) <i>EMBO J</i> <i></i> <i></i> 21: 5 920-929<br/>

http://publicationslist.org/joaquin.arino/refid28 2008-04-19T08:48:20Z

The inclusion of genetically modified (GM) foods in the human diet has caused considerable debate. There is concern that the transfer of plant-derived transgenes to the resident intestinal microflora could have safety implications. For these gene transfer events to occur, the nucleic acid would need to survive passage through the gastrointestinal tract. The aim of the present study was to evaluate...<br/><br/>Susana M Martín-Orúe, Anthony G O'Donnell, Joaquin Ariño, Trudy Netherwood, Harry J Gilbert, John C Mathers (2002) <i>Br J Nutr</i> <i></i> <i></i> 87: 6 533-542<br/>

http://publicationslist.org/joaquin.arino/refid27 2008-04-19T08:48:20Z

Maintenance of cellular integrity in Saccharomyces cerevisiae is carried out by the activation of the protein kinase C-mediated mitogen-activated protein kinase (PKC1-MAPK) pathway. Here we report that correct down-regulation of both basal and induced activity of the PKC1-MAPK pathway requires the SIT4 function. Sit4 is a protein phosphatase also required for a proper cell cycle progression. We pr...<br/><br/>Maria Angeles de la Torre-Ruiz, Jordi Torres, Joaquin Arino, Enrique Herrero (2002) <i>J Biol Chem</i> <i></i> <i></i> 277: 36 33468-33476<br/>

http://publicationslist.org/joaquin.arino/refid26 2008-04-19T08:48:20Z

The short-time transcriptional response of yeast cells to a mild increase in external pH (7.6) has been investigated using DNA microarrays. A total of 150 genes increased their mRNA level at least twofold within 45 min. Alkalinization resulted in the repression of 232 genes. The response of four upregulated genes, ENA1 (encoding a Na+-ATPase also induced by saline stress) and PHO84, PHO89 and PHO1...<br/><br/>Raquel Serrano, Amparo Ruiz, Dolores Bernal, James R Chambers, Joaquín Ariño (2002) <i>Mol Microbiol</i> <i></i> <i></i> 46: 5 1319-1333<br/>

http://publicationslist.org/joaquin.arino/refid37 2008-04-19T08:48:20Z

The gene pzl-1 from the filamentous fungus Neurospora crassa encodes a putative Ser/Thr protein phosphatase that is reminiscent of the Ppz1/Ppz2 and Pzh1 phosphatases from Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. The entire PZL-1 protein, as well as its carboxyl-terminal domain, have been expressed in Escherichia coli as active protein phosphatases. To characterize its...<br/><br/>E Vissi, J Clotet, E de Nadal, A Barceló, E Bakó , P Gergely, V Dombrádi, J Ariño (2001) <i>Yeast</i> <i></i> <i></i> 18: 2 115-124<br/>

http://publicationslist.org/joaquin.arino/refid35 2008-04-19T08:48:20Z

Exposure of yeast to increases in extracellular osmolarity activates the Hog1 mitogen-activated protein kinase (MAPK), which is essential for the induction of gene expression required for cell survival upon osmotic stress. Several genes are regulated in response to osmotic stress by Sko1, a transcriptional repressor of the ATF/CREB family. We show by in vivo coprecipitation and phosphorylation stu...<br/><br/>M Proft, A Pascual-Ahuir, E de Nadal, J Ariño, R Serrano, F Posas (2001) <i>EMBO J</i> <i></i> <i></i> 20: 5 1123-1133<br/>

http://publicationslist.org/joaquin.arino/refid34 2008-04-19T08:48:20Z

Biochemical and crystallographic data suggest that, in contrast with other organisms, the active maize protein kinase CK2 might be composed simply of a catalytic polypeptide (CK2alpha), thus lacking CK2beta regulatory subunits. To investigate the existence and functionality of CK2beta regulatory subunits in Zea mays, we have screened a maize cDNA library using different approaches and have isolate...<br/><br/>M Riera, G Peracchia, E de Nadal, J Ariño, M Pagès (2001) <i>Plant J</i> <i></i> <i></i> 25: 4 365-374<br/>

http://publicationslist.org/joaquin.arino/refid33 2008-04-19T08:48:20Z

In vivo 32P-labeled yeast proteins from wild type and ppz1 ppz2 phosphatase mutants were resolved by bidimensional electrophoresis. A prominent phosphoprotein, which in ppz mutants showed a marked shift to acidic regions, was identified by mixed peptide sequencing as the translation elongation factor 1Balpha (formerly eEF1beta). An equivalent shift was detected in cells overexpressing HAL3, a inhi...<br/><br/>E de Nadal, R P Fadden, A Ruiz, T Haystead, J Ariño (2001) <i>J Biol Chem</i> <i></i> <i></i> 276: 18 14829-14834<br/>

http://publicationslist.org/joaquin.arino/refid32 2008-04-19T08:48:20Z

A screening for multicopy suppressors of the G(1)/S blockage of a conditional sit4 hal3 mutant yielded the NHA1 gene, encoding a Na(+),K(+)/H(+) antiporter, composed of a transmembrane domain and a large carboxyl-terminal tail, which has been related to cation detoxification processes. Expression of either the powerful Saccharomyces cerevisiae Ena1 Na(+)/H(+)-ATPase or the Schizosaccharomyces pomb...<br/><br/>E Simón, J Clotet, F Calero, J Ramos, J Ariño (2001) <i>J Biol Chem</i> <i></i> <i></i> 276: 32 29740-29747<br/>

http://publicationslist.org/joaquin.arino/refid41 2008-04-19T08:48:20Z

Adaptation to changes in extracellular salinity is a critical event for cell survival. Genome-wide DNA chip analysis has been used to analyze the transcriptional response of yeast cells to saline stress. About 7% of the genes encoded in the yeast genome are induced more than 5-fold after a mild and brief saline shock (0.4 m NaCl, 10 min). Interestingly, most responsive genes showed a very transien...<br/><br/>F Posas, J R Chambers, J A Heyman, J P Hoeffler, E de Nadal, J Ariño (2000) <i>J Biol Chem</i> <i></i> <i></i> 275: 23 17249-17255<br/>

http://publicationslist.org/joaquin.arino/refid42 2008-04-19T08:48:20Z

Carnitine octanoyltransferase (COT), an enzyme that facilitates the transport of medium chain fatty acids through peroxisomal membranes, is inhibited by malonyl-CoA. cDNAs encoding full-length wild-type COT and one double mutant variant from rat peroxisomal COT were expressed in Saccharomyces cerevisiae. Both expressed forms were expressed similarly in quantitative terms and exhibited full enzyme ...<br/><br/>M Morillas, J Clotet, B Rubí, D Serra, G Asins, J Ariño, F G Hegardt (2000) <i>FEBS Lett</i> <i></i> <i></i> 466: 1 183-186<br/>

http://publicationslist.org/joaquin.arino/refid43 2008-04-19T08:48:20Z

The trk1(+) gene has been proposed as a component of the K(+) influx system in the fission yeast Schizosaccharomyces pombe. Previous work from our laboratories revealed that trk1 mutants do not show significantly altered content or influx of K(+), although they are more sensitive to Na(+). Genome database searches revealed that S. pombe encodes a putative gene (designated here trk2(+)) that shows ...<br/><br/>F Calero, N Gómez, J Ariño, J Ramos (2000) <i>J Bacteriol</i> <i></i> <i></i> 182: 2 394-399<br/>

http://publicationslist.org/joaquin.arino/refid40 2008-04-19T08:48:20Z

Exposure of yeast cells to increases in extracellular osmolarity activates the Hog1 mitogen-activated protein kinase (MAPK). Activation of Hog1 MAPK results in induction of a set of osmoadaptive responses, which allow cells to survive in high-osmolarity environments. Little is known about how the MAPK activation results in induction of these responses, mainly because no direct substrates for Hog1 ...<br/><br/>E Bilsland-Marchesan, J Ariño, H Saito, P Sunnerhagen, F Posas (2000) <i>Mol Cell Biol</i> <i></i> <i></i> 20: 11 3887-3895<br/>

http://publicationslist.org/joaquin.arino/refid39 2008-04-19T08:48:20Z

Rat peroxisomal carnitine octanoyltransferase (COT), which facilitates the transport of medium-chain fatty acids through the peroxisomal membrane, is irreversibly inhibited by the hypoglycaemia-inducing drug etomoxir. To identify the molecular basis of this inhibition, cDNAs encoding full-length wild-type COT, two different variant point mutants and one variant double mutant from rat peroxisomal C...<br/><br/>M Morillas, J Clotet, B Rubí, D Serra, J Ariño, F G Hegardt, G Asins (2000) <i>Biochem J</i> <i></i> <i></i> 351 Pt 2: 495-502<br/>

http://publicationslist.org/joaquin.arino/refid38 2008-04-19T08:48:20Z

We detected an about 200 kDa holoenzyme of protein phosphatase 2A (PP2A) in the crude extract of Medicago sativa microcallus cells by gel permeation chromatography. By polymerase chain reaction (PCR) we isolated two M. sativa cDNA fragments corresponding to the catalytic (C) subunit, and one each coding for the A and the B regulatory subunits of PP2A. The C subunit sequences were different from th...<br/><br/>E C Tóth, E Vissi, I Kovács, A Szöke, J Ariño, P Gergely, D Dudits, V Dombrádi (2000) <i>Plant Mol Biol</i> <i></i> <i></i> 43: 4 527-536<br/>

http://publicationslist.org/joaquin.arino/refid36 2008-04-19T08:48:20Z

The PPX/PP4 Ser/Thr protein phosphatases belong to the type 2A phosphatase subfamily and are present in most eukaryotic organisms. We have previously isolated two closely related DNAs encoding PPX isoforms (PPX-1 and PPX-2) of Arabidopsis thaliana. Here we report the molecular cloning of the genes encoding these proteins. The genes PPX-1 and PPX-2 are composed of eight exons and seven introns loca...<br/><br/>G Pujol, T I Baskin, A Casamayor, N Cortadellas, A Ferrer, J Ariño (2000) <i>Plant Mol Biol</i> <i></i> <i></i> 44: 4 499-511<br/>

http://publicationslist.org/joaquin.arino/refid44 2008-04-19T08:48:20Z

Saccharomyces cerevisiae cells lacking the regulatory subunit of casein kinase 2 (CK-2), encoded by the gene CKB1, display a phenotype of hypersensitivity to Na(+) and Li(+) cations. The sensitivity of a strain lacking ckb1 is higher than that of a calcineurin mutant and similar to that of a strain lacking HAL3, the regulatory subunit of the Ppz1 protein phosphatase. Genetic analysis indicated tha...<br/><br/>E de Nadal, F Calero, J Ramos, J Ariño (1999) <i>J Bacteriol</i> <i></i> <i></i> 181: 20 6456-6462<br/>

http://publicationslist.org/joaquin.arino/refid45 2008-04-19T08:48:20Z

We have disrupted seven open reading frames (ORFs) located in the left arm of chromosome XV of the yeast Saccharomyces cerevisiae. These ORFs, previously discovered by our laboratory during the programme of systematic sequencing of the yeast genome, are YOL152w, YOL151w, YOL149w, YOL130w, YOL128c, YOL125w and YOL124c. In most cases, the short flanking homology (SFH) replacement technique has been ...<br/><br/>K Hajji, J Clotet, J Ariño (1999) <i>Yeast</i> <i></i> <i></i> 15: 5 435-441<br/>

http://publicationslist.org/joaquin.arino/refid46 2008-04-19T08:48:20Z

Yeast cells overexpressing the Ser/Thr protein phosphatase Ppz1 display a slow-growth phenotype. These cells recover slowly from alpha-factor or nutrient depletion-induced G1 arrest, showing a considerable delay in bud emergence as well as in the expression of the G1 cyclins Cln2 and Clb5. Therefore, an excess of the Ppz1 phosphatase interferes with the normal transition from G1 to S phase. The gr...<br/><br/>J Clotet, E Garí, M Aldea, J Ariño (1999) <i>Mol Cell Biol</i> <i></i> <i></i> 19: 3 2408-2415<br/>

http://publicationslist.org/joaquin.arino/refid47 2008-04-19T08:48:20Z

We have previously shown that fission yeast encodes a PPZ-like phosphatase, designated Pzhl, which is an important determinant of cation homeostasis. pzh1 delta mutants display increased tolerance to Na+ ions, but they are hypersensitive to KC1 [Balcells, L., Gómez, N., Casamayor, A., Clotet, J. & Ariño, J. (1997) Eur. J. Biochem. 250, 476-483]. We have immunodetected Pzh1 in yeast extracts and ...<br/><br/>L Balcells, F Calero, N Gómez, J Ramos, J Ariño (1999) <i>Eur J Biochem</i> <i></i> <i></i> 260: 1 31-37<br/>

http://publicationslist.org/joaquin.arino/refid52 2008-04-19T08:48:20Z

The plant Arabidopsis thaliana contains five isoforms of the catalytic subunit of protein phosphatase 2A (PP2A) that can be grouped into two families, one composed by isoforms PP2A-1, -2 and -5 and the other composed by isoforms PP2A-3 and PP2A-4. An Arabidopsis genomic library was screened and several clones corresponding to genes PP2A-3 and PP2A-4 were isolated and analysed. Both genes span over...<br/><br/>E Pérez-Callejón, A Casamayor, G Pujol, M Camps, A Ferrer, J Ariño (1998) <i>Gene</i> <i></i> <i></i> 209: 1-2 105-112<br/>

http://publicationslist.org/joaquin.arino/refid53 2008-04-19T08:48:20Z

The open reading frame YGL087c in the budding yeast Saccharomyces cerevisiae genome encodes a polypeptide highly similar to the human UEV (ubiquitin-conjugating E2 enzyme variant) proteins, which have been proposed to belong to a family of putative dominant negative ubiquitin regulators. Deletion of the YGL087c open reading frame yields viable cells which are sensitive to UV irradiation or methyl ...<br/><br/>T M Thomson, H Khalid, J J Lozano, E Sancho, J Ariño (1998) <i>FEBS Lett</i> <i></i> <i></i> 423: 1 49-52<br/>

http://publicationslist.org/joaquin.arino/refid51 2008-04-19T08:48:20Z

Components of cellular stress responses can be identified by correlating changes in stress tolerance with gain or loss of function of defined genes. Previous work has shown that yeast cells deficient in Ppz1 protein phosphatase or overexpressing Hal3p, a novel regulatory protein of unknown function, exhibit increased resistance to sodium and lithium, whereas cells lacking Hal3p display increased s...<br/><br/>E de Nadal, J Clotet, F Posas, R Serrano, N Gomez, J Ariño (1998) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 95: 13 7357-7362<br/>

http://publicationslist.org/joaquin.arino/refid49 2008-04-19T08:48:20Z

J Ariño, F Posas, J Clotet (1998) <i>Methods Mol Biol</i> <i></i> <i></i> 93: 305-313<br/>

http://publicationslist.org/joaquin.arino/refid50 2008-04-19T08:48:20Z

G Pujol, A Ferrer, J Ariño (1998) <i>Methods Mol Biol</i> <i></i> <i></i> 93: 201-212<br/>

http://publicationslist.org/joaquin.arino/refid48 2008-04-19T08:48:20Z

We have previously shown that the mutation of the Schizosaccharomyces pombe PPZ-like protein phosphatase encoded by the gene pzh1+ results in increased tolerance to sodium and in hypersensitivity to potassium ions. A similar phenotype has also been reported for deletants in the spm1/pmk1 gene, encoding a mitogen-activated protein (MAP) kinase. We have found that the sodium tolerance phenotype of p...<br/><br/>L Balcells, R Martín, M C Ruiz, N Gómez, J Ramos, J Ariño (1998) <i>FEBS Lett</i> <i></i> <i></i> 435: 2-3 241-244<br/>

http://publicationslist.org/joaquin.arino/refid55 2008-04-19T08:48:20Z

Chromosome XV was one of the last two chromosomes of Saccharomyces cerevisiae to be discovered. It is the third-largest yeast chromosome after chromosomes XII and IV, and is very similar in size to chromosome VII. It alone represents 9% of the yeast genome (8% if ribosomal DNA is included). When systematic sequencing of chromosome XV was started, 93 genes or markers were identified, and most of th...<br/><br/>B Dujon, K Albermann, M Aldea, D Alexandraki, W Ansorge, J Arino, V Benes, C Bohn, M Bolotin-Fukuhara, R Bordonné, J Boyer, A Camasses, A Casamayor, C Casas, G Chéret, C Cziepluch, B Daignan-Fornier, D V Dang, M de Haan, H Delius, P Durand, C Fairhead, H Feldmann, L Gaillon, K Kleine (1997) <i>Nature</i> <i></i> <i></i> 387: 6632 Suppl 98-102<br/>

http://publicationslist.org/joaquin.arino/refid54 2008-04-19T08:48:20Z

In the yeast Saccharomyces cerevisiae, Na+ efflux is mediated by the Ena1 ATPase, and the expression of the ENA1 gene is regulated by the Ppz1 and Ppz2 Ser/Thr protein phosphatases. On the contrary, in the fission yeast Schizosaccharomyces pombe, effective output of Na+ is attributed to the H+/Na+ antiporter encoded by the sod2 gene. We have isolated a S. pombe gene (pzh1) that encodes a 515-amino...<br/><br/>L Balcells, N Gómez, A Casamayor, J Clotet, J Ariño (1997) <i>Eur J Biochem</i> <i></i> <i></i> 250: 2 476-483<br/>

http://publicationslist.org/joaquin.arino/refid58 2008-04-19T08:48:20Z

A 13421 bp fragment located near the left telomere of chromosome XV (cosmid pEOA461) has been sequenced. Seven non-overlapping open reading frames (ORFs) encoding polypeptides longer than 100 residues have been found (AOB859, AOC184, AOE375, AOX142i, AOE423, AOA476 and AOE433). An additional ORF (AOE131) is found within AOA476. Three of them (AOC184, AOA476 and AOE433) show no remarkable identity ...<br/><br/>A Casamayor, H Khalid, L Balcells, M Aldea, C Casas, E Herrero, J Ariño (1996) <i>Yeast</i> <i></i> <i></i> 12: 10B Suppl 1013-1020<br/>

http://publicationslist.org/joaquin.arino/refid57 2008-04-19T08:48:20Z

The DNA sequence of a 12,801 bp fragment located near the left telomere of chromosome XV has been determined. Sequence analysis reveals eight open reading frames (ORFs) encoding polypeptides larger than 100 residues. ORFs AOE129 and AOAA121 are in opposite strands and they overlap at their 3' ends. AOE397 has similarity with phosphofructokinase genes from other organisms and may code for a second ...<br/><br/>M Aldea, L Piedrafita, C Casas, A Casamayor, H Khalid, L Balcells, J Ariño, E Herrero (1996) <i>Yeast</i> <i></i> <i></i> 12: 10B Suppl 1053-1058<br/>

http://publicationslist.org/joaquin.arino/refid59 2008-04-19T08:48:20Z

We report the sequence of a 15.5 kb DNA segment located near the left telomere of chromosome XV of Saccharomyces cerevisiae. The sequence contains nine open reading frames (ORFs) longer than 300 bp. Three of them are internal to other ones. One corresponds to the gene LGT3 that encodes a putative sugar transporter. Three adjacent ORFs were separated by two stop codons in frame. These ORFs presente...<br/><br/>F J Gamo, M J Lafuente, A Casamayor, J Ariño, M Aldea, C Casas, E Herrero, C Gancedo (1996) <i>Yeast</i> <i></i> <i></i> 12: 7 709-714<br/>

http://publicationslist.org/joaquin.arino/refid56 2008-04-19T08:48:20Z

Deletion of the yeast Ser/Thr protein phosphatase PPZ1 results in increased tolerance to sodium and lithium. PPZ1 is also important for cell integrity, as ppz1Delta cells undergo lysis under caffeine stress and PPZ1 overexpression overrides the lytic defect of mutants in the protein kinase C/mitogen-activated protein (MAP) kinase pathway. The PPZ1 protein can be dissected in two halves. The COOH-t...<br/><br/>J Clotet, F Posas, E de Nadal, J Ariño (1996) <i>J Biol Chem</i> <i></i> <i></i> 271: 42 26349-26355<br/>

http://publicationslist.org/joaquin.arino/refid63 2008-04-19T08:48:20Z

Protein phosphatases PPZ1 and PPZ2 represent a novel form of Ser/Thr phosphatases structurally related to type 1 phosphatases and characterized by an unusual amino-terminal region. We have found that the deletion of PPZ1 gene results in increased tolerance to Na+ and Li+ cations. Simultaneous deletion of PPZ2 gene results in an additional increase in salt tolerance. After exposure to high concentr...<br/><br/>F Posas, M Camps, J Ariño (1995) <i>J Biol Chem</i> <i></i> <i></i> 270: 22 13036-13041<br/>

http://publicationslist.org/joaquin.arino/refid64 2008-04-19T08:48:20Z

The yeast homologues of mammalian protein phosphatase 2A (PP2A) are encoded by two genes, PPH21 and PPH22. To evaluate the role of these phosphatases in the control of glycogen metabolism, wild-type cells and mutants carrying deletions of PPH21 or PPH22 were studied. Our results indicate that the lack of a single gene product does not result in significant changes in glycogen content, glycogen syn...<br/><br/>J Clotet, F Posas, G Z Hu, H Ronne, J Ariño (1995) <i>Eur J Biochem</i> <i></i> <i></i> 229: 1 207-214<br/>

http://publicationslist.org/joaquin.arino/refid62 2008-04-19T08:48:20Z

The Saccharomyces cerevisiae gene PPZ1 codes for a 692-residues protein that shows in its carboxyl-terminal half about 60% identity with the catalytic subunit of mammalian and yeast protein phosphatase-1 and that is involved in salt homeostasis. The complete PPZ1 protein has been successfully expressed as a soluble glutathione-S-transferase fusion protein. The recombinant protein, after purificati...<br/><br/>F Posas, M Bollen, W Stalmans, J Ariño (1995) <i>FEBS Lett</i> <i></i> <i></i> 368: 1 39-44<br/>

http://publicationslist.org/joaquin.arino/refid61 2008-04-19T08:48:20Z

The DNA sequence of a 9873 bp fragment located near the left telomere of chromosome XV has been determined. Sequence analysis reveals seven open reading frames. One is the ARG8 gene coding for N-acetylornithine aminotransferase. Another corresponds to CDC33, which codes for the initiation factor 4E or cap binding protein. The open reading frame AOE169 can be considered as the putative gene for the...<br/><br/>C Casas, M Aldea, A Casamayor, M J Lafuente, F J Gamo, C Gancedo, J Ariño, E Herrero (1995) <i>Yeast</i> <i></i> <i></i> 11: 11 1061-1067<br/>

http://publicationslist.org/joaquin.arino/refid60 2008-04-19T08:48:20Z

The sequence of a 13 kbp fragment located in the vicinity of the left telomere of chromosome XV (cosmid pEOA179) has been determined. Seven new open reading frames (ORFs) encoding polypeptides longer than 100 residues have been found (AOB629, AOA342, AOC231, AOE555, AOE236, AOA236 and AOE1045). Three of them show no identity with proteins deposited in the data banks. ORF AOB629 (629 amino acids) h...<br/><br/>A Casamayor, M Aldea, C Casas, E Herrero, F J Gamo, M J Lafuente, C Gancedo, J Ariño (1995) <i>Yeast</i> <i></i> <i></i> 11: 13 1281-1288<br/>

http://publicationslist.org/joaquin.arino/refid65 2008-04-19T08:48:20Z

Incubation of hepatocytes from diabetic rats with glucose results in the translocation of glycogen synthase from soluble fractions to fractions which sediment at 9200 g. The extent of the translocation correlates positively with the intracellular concentration of glucose 6-phosphate. No difference was found between healthy and diabetic rats in the capacity of glycogen synthase to translocate to pe...<br/><br/>J M Fernández-Novell, J Ariño, J J Guinovart (1994) <i>Eur J Biochem</i> <i></i> <i></i> 226: 2 665-671<br/>

http://publicationslist.org/joaquin.arino/refid66 2008-04-19T08:48:20Z

We have recently reported the existence of multiple isoforms of the catalytic subunit of protein phosphatase 2A (PP2A) in Arabidopsis thaliana and the molecular cloning of cDNAs encoding three of these proteins (PP2A-1, PP2A-2, PP2A-3). The reported cDNA encoding PP2A-3 was truncated at the 5' terminus, lacking a short fragment of the N-terminal coding sequence. We have now isolated a near full-le...<br/><br/>A Casamayor, E Pérez-Callejón, G Pujol, J Ariño, A Ferrer (1994) <i>Plant Mol Biol</i> <i></i> <i></i> 26: 1 523-528<br/>

http://publicationslist.org/joaquin.arino/refid69 2008-04-19T08:48:20Z

Two DNA fragments, AP-1 and AP-2, encoding amino acid sequences closely related to Ser/Thr protein phosphatases were amplified from Arabidopsis thaliana genomic DNA. Fragment AP-1 was used to screen A. thaliana cDNA libraries and several positive clones were isolated. Clones EP8a and EP14a were sequenced and found to encode almost identical proteins (97% identity). Both proteins are 306 amino acid...<br/><br/>J Ariño, E Pérez-Callejón, N Cunillera, M Camps, F Posas, A Ferrer (1993) <i>Plant Mol Biol</i> <i></i> <i></i> 21: 3 475-485<br/>

http://publicationslist.org/joaquin.arino/refid68 2008-04-19T08:48:20Z

We have recently reported the existence in the yeast Saccharomyces cerevisiae of a gene named PPZ1, encoding a novel Ser/Thr phosphatase characterized by a large, Ser-rich amino-terminal extension, and suggested the existence of a related gene product that could have overlapping functions. We have now amplified by polymerase chain reaction techniques a genomic fragment of about 600 bp correspondin...<br/><br/>F Posas, A Casamayor, J Ariño (1993) <i>FEBS Lett</i> <i></i> <i></i> 318: 3 282-286<br/>

http://publicationslist.org/joaquin.arino/refid70 2008-04-19T08:48:20Z

Degenerate oligonucleotides were used to selectively amplify yeast genomic sequences related to Ser/Thr protein phosphatases. Among the sequences obtained, clone ST4-2 was found to code for a novel sequence related to previously known phosphatases. A size-selected yeast genomic library was constructed and screened using clone ST4-2 as probe, and one positive clone, named PPG, was isolated. DNA seq...<br/><br/>F Posas, J Clotet, M T Muns, J Corominas, A Casamayor, J Ariño (1993) <i>J Biol Chem</i> <i></i> <i></i> 268: 2 1349-1354<br/>

http://publicationslist.org/joaquin.arino/refid67 2008-04-19T08:48:20Z

In a recent paper [Ariño et al., Plant Mol Biol 21: 475-485 (1993)] we reported the amplification of a DNA fragment (AP-2) from the genome of Arabidopsis thaliana encoding an amino acid sequence corresponding to a Ser/Thr protein phosphatase distantly related to type 2A protein phosphatases. In this paper we report the use of the AP-2 fragment to isolate several cDNA clones from a leaf cDNA libra...<br/><br/>E Pérez-Callejón, A Casamayor, G Pujol, E Clua, A Ferrer, J Ariño (1993) <i>Plant Mol Biol</i> <i></i> <i></i> 23: 6 1177-1185<br/>

http://publicationslist.org/joaquin.arino/refid74 2008-04-19T08:48:20Z

Incubation of rat hepatocytes with glucose results in a decrease in the amount of glycogen synthase activity found in supernatants obtained after centrifugation of cell homogenates at 9200 g. The enzymic activity was quantitatively recovered in the sediments. This effect of translocation was dose- and time-dependent and correlated with the amount of immunoreactive enzyme determined by immunoblotti...<br/><br/>J M Fernández-Novell, J Ariño, S Vilaró, J J Guinovart (1992) <i>Biochem J</i> <i></i> <i></i> 281 ( Pt 2): 443-448<br/>

http://publicationslist.org/joaquin.arino/refid73 2008-04-19T08:48:20Z

DNA fragments containing structural characteristics found in Ser/Thr protein phosphatases were amplified by polymerase chain reaction from yeast genome. Amplification was carried out by using degenerate oligonucleotides encoding conserved sequences found in type 1, 2A, and 2B phosphatases. A 215-base pair amplification fragment was used to screen a size-selected library, and a positive clone was i...<br/><br/>F Posas, A Casamayor, N Morral, J Ariño (1992) <i>J Biol Chem</i> <i></i> <i></i> 267: 17 11734-11740<br/>

http://publicationslist.org/joaquin.arino/refid71 2008-04-19T08:48:20Z

Incubation of rat hepatocytes with glucose induces the translocation of glycogen synthase from soluble fractions to fractions which sediment at 10,000 g. Incubation of the cells with fructose, galactose, 2-deoxyglucose or 5-thioglucose, which activate glycogen synthase, also resulted in the translocation of the enzyme, whereas 3-O-methylglucose, 6-deoxyglucose and 1,5-anhydroglucitol, which do not...<br/><br/>J M Fernández-Novell, J Ariño, S Vilaró, D Bellido, J J Guinovart (1992) <i>Biochem J</i> <i></i> <i></i> 288 ( Pt 2): 497-501<br/>

http://publicationslist.org/joaquin.arino/refid72 2008-04-19T08:48:20Z

Disruption of the gene pgil of Saccharomyces cerevisiae, which codes for phosphoglucose isomerase, results in a dramatic increase in the amount of intracellular glycogen in early exponential cultures. The level of glucose 6-phosphate was much higher in mutant than in wild-type cells. Phosphorylase a activity and the state of activation of glycogen synthase were also investigated. Phosphorylase a a...<br/><br/>J Corominas, J Clotet, I Fernández-Bañares, E Boles, F K Zimmermann, J J Guinovart, J Ariño (1992) <i>FEBS Lett</i> <i></i> <i></i> 310: 2 182-186<br/>

http://publicationslist.org/joaquin.arino/refid77 2008-04-19T08:48:20Z

The gene SIT4 of S. cerevisiae, which codes for a protein structurally related to the catalytic subunit of mammalian protein phosphatase 2A, was disrupted in vitro. Analysis of glycogen synthase activity ratio in mutant haploid cells indicated that the enzyme was less active than in wild-type cells. On the contrary, glycogen phosphorylase alpha activity was much higher. The activation of glycogen ...<br/><br/>F Posas, J Clotet, J Ariño (1991) <i>FEBS Lett</i> <i></i> <i></i> 279: 2 341-345<br/>

http://publicationslist.org/joaquin.arino/refid76 2008-04-19T08:48:20Z

S. cerevisiae gene DIS2S1, which codes for a protein very similar to the catalytic subunit of mammalian protein phosphatase 1, was disrupted "in vitro". Diploid yeast cells were transformed and sporulated. Tetrad analysis demonstrated that disruption of DIS2S1 is lethal for the cell. Glycogen phosphorylase alpha and glycogen synthase activity ratio were measured in diploids carrying a disrupted al...<br/><br/>J Clotet, F Posas, A Casamayor, I Schaaff-Gerstenschläger, J Ariño (1991) <i>Curr Genet</i> <i></i> <i></i> 19: 5 339-342<br/>

http://publicationslist.org/joaquin.arino/refid75 2008-04-19T08:48:20Z

The mechanism by which yeast ras2 mutant hyperaccumulates glycogen has been investigated. Total glycogen synthase activity was between 2.5 and 1.3 times higher in the ras2 mutant than in an isogenic strain. In addition, while in the normal strain the glycogen synthase activation state decreased along the exponential phase, in the mutant strain the opposite behaviour was observed: glycogen synthase...<br/><br/>I Fernández-Bañares, J Clotet, J Ariño, J J Guinovart (1991) <i>FEBS Lett</i> <i></i> <i></i> 290: 1-2 38-42<br/>

http://publicationslist.org/joaquin.arino/refid78 2008-04-19T08:50:27Z

Glycogen synthase from Saccharomyces cerevisiae was purified to homogeneity. The enzyme showed a subunit molecular mass of 80 kDa. The holoenzyme appears to be a tetramer. Antibodies developed against purified yeast glycogen synthase inactivated the enzyme in yeast extracts and allowed the detection of the protein in Western blots. Amino acid analysis showed that the enzyme is very rich in glutama...<br/><br/>A Carabaza, J Arino, J W Fox, C Villar-Palasi, J J Guinovart (1990) <i>Biochem J</i> <i></i> <i></i> 268: 2 401-407<br/>

http://publicationslist.org/joaquin.arino/refid80 2008-04-19T08:50:27Z

F Posas, J Ariño (1989) <i>Nucleic Acids Res</i> <i></i> <i></i> 17: 20 <br/>

http://publicationslist.org/joaquin.arino/refid81 2008-04-19T08:50:27Z

Although glycogen synthase is present in a highly inactivated state in hepatocytes from streptozocin-induced diabetic rats, glucagon, vasopressin, and vanadate are still able to further decrease the basal activity of the enzyme. This inactivation was observed with the low-to-high glucose 6-phosphate activity ratio assay. The inactivation of glycogen synthase occurred concomitantly with the activat...<br/><br/>J E Rodríguez-Gil, A M Gómez-Foix, J Ariño, J J Guinovart, F Bosch (1989) <i>Diabetes</i> <i></i> <i></i> 38: 6 793-798<br/>

http://publicationslist.org/joaquin.arino/refid79 2008-04-19T08:50:27Z

F Posas, J Ariño (1989) <i>Nucleic Acids Res</i> <i></i> <i></i> 17: 20 <br/>

http://publicationslist.org/joaquin.arino/refid82 2008-04-19T08:50:27Z

Two cDNA clones were isolated from a human liver library that encode two phosphatase 2A catalytic subunits. The two cDNAs differed in eight amino acids (97% identity) with three nonconservative substitutions. All of the amino acid substitutions were clustered in the amino-terminal domain of the protein. Amino acid sequence of one human liver clone (HL-14) was identical to the rabbit skeletal muscl...<br/><br/>J Arino, C W Woon, D L Brautigan, T B Miller, G L Johnson (1988) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 85: 12 4252-4256<br/>

http://publicationslist.org/joaquin.arino/refid84 2008-04-19T08:51:02Z

Vanadate inactivated rat hepatocyte glycogen synthase and activated glycogen phosphorylase in a dose- and time-dependent manner. These effects were observed in hepatocytes from both fasted as well as fed rats. When rat hepatocytes were preincubated with [32P]phosphate and then with vanadate, and the 32P-labeled glycogen synthase was specifically immunoprecipitated, it was observed that vanadate st...<br/><br/>F Bosch, J Ariño, A M Gómez-Foix, J J Guinovart (1987) <i>J Biol Chem</i> <i></i> <i></i> 262: 1 218-222<br/>

http://publicationslist.org/joaquin.arino/refid83 2008-04-19T08:50:27Z

The Mr = 33,000 catalytic fragment of rabbit skeletal muscle type 1 protein phosphatase was digested with trypsin after reduction and alkylation. The resulting peptides were isolated, subjected to automated Edman degradation, and their sequences compared to the deduced peptide sequence of the bovine type 2A protein phosphatase cDNA. Of 10 tryptic peptides from the type 1 phosphatase that were sequ...<br/><br/>G L Johnson, D L Brautigan, C Shriner, S Jaspers, J Arino, J E Mole, T B Miller, M C Mumby (1987) <i>Mol Endocrinol</i> <i></i> <i></i> 1: 10 745-748<br/>

http://publicationslist.org/joaquin.arino/refid86 2008-04-19T08:51:02Z

Incubation of rat hepatocytes with active phorbol esters and mezerein provoked a decrease in glycogen synthase activity. After the incubation of [3 2 P] phosphate-labeled cells with these tumor promoters, an increase in the amount of 3 2 P bound to the immunoprecipitated enzyme was observed. The decrease in activity highly correlated with the phosphorylation in the smaller CNBr fragment (CB-1) and...<br/><br/>J Ariño, J J Guinovart (1986) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 134: 1 113-119<br/>

http://publicationslist.org/joaquin.arino/refid85 2008-04-19T08:51:02Z

Incubation of hepatocytes from fasted rats with LiCl provoked a concentration- and time-dependent activation of glycogen synthase. This effect was observed in the absence of glucose in the incubation medium. No changes in the intracellular concentrations of ATP or glucose-6-phosphate were detected. Lithium was also able to activate glycogen synthase in the absence of extracellular calcium. If hepa...<br/><br/>F Bosch, A M Gómez-Foix, J Ariño, J J Guinovart (1986) <i>J Biol Chem</i> <i></i> <i></i> 261: 36 16927-16931<br/>

http://publicationslist.org/joaquin.arino/refid87 2008-04-19T08:51:02Z

32P-labeled glycogen synthase specifically immunoprecipitated from 32P-phosphate incubated rat hepatocytes contains, in addition to [32P] phosphoserine, significant levels of [32P] phosphothreonine (7% of the total [32P] phosphoaminoacids). When the 32P-immunoprecipitate was cleaved with CNBr, the [32P] phosphothreonine was recovered in the large CNBr fragment (CB-2, Mapp 28 Kd). Homogeneous rat l...<br/><br/>J Ariño, M Arró, J J Guinovart (1985) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 130: 3 987-993<br/>

http://publicationslist.org/joaquin.arino/refid88 2008-04-19T08:51:02Z

The effects of epinephrine and vasopressin on the phosphorylation state of glycogen synthase were studied using rat hepatocytes incubated with 32P. After the incubation with hormones, 32P-labeled glycogen synthase was isolated using antibodies against rat liver enzyme. The immunoprecipitate showed a single radioactive band ( Mapp 88 kDa) when subjected to SDS-gel electrophoresis. Both epinephrine ...<br/><br/>J Ariño, A Mor, F Bosch, I V Baanante, J J Guinovart (1984) <i>FEBS Lett</i> <i></i> <i></i> 170: 2 310-314<br/>