Jüri Jarvet's Publications List

Secondary structure conversions of Alzheimer's Abeta(1-40) peptide induced by membrane-mimicking detergents.

2011-05-05T08:40:23Z

The amyloid beta peptide (Abeta) with 39-42 residues is the major component of amyloid plaques found in brains of Alzheimer's disease patients, and soluble oligomeric peptide aggregates mediate toxic effects on neurons. The Abeta aggregation involves a conformational change of the peptide structure to beta-sheet. In the present study, we report on the effect of detergents on the structure transiti...

Anna Wahlström, Loïc Hugonin, Alex Perálvarez-Marín, Jüri Jarvet, Astrid Gräslund (2008) FEBS J 275: 20 5117-5128

Making a single-chain four-helix bundle for redox chemistry studies.

2011-05-05T08:40:23Z

The construction and characteristics of the stable and well-structured alpha(4)W protein are described. The 117-residue, single-chain protein has a molecular weight of 13.1 kDa and is designed to fold into a four-helix bundle. Experimental characterization of the expressed and purified protein shows a 69.8 +/- 0.8% helical content over a 5.5-10.0 pH range. The protein is thermostable with a T(M) >...

Kristina Westerlund, Sean D Moran, Heidi K Privett, Sam Hay, Jüri Jarvet, Brian R Gibney, Cecilia Tommos (2008) Protein Eng Des Sel 21: 11 645-652

Maximum entropy reconstruction of joint phi, psi-distribution with a coil-library prior: the backbone conformation of the peptide hormone motilin in aqueous solution from phi and psi-dependent J-couplings.

2011-05-05T08:40:23Z

In this paper, we present a new method for structure determination of flexible "random-coil" peptides. A numerical method is described, where the experimentally measured 3J(H(alpha)Nalpha) and [3J(H(alpha)Nalpha+1 couplings, which depend on the phi and psi dihedral angles, are analyzed jointly with the information from a coil-library through a maximum entropy approach. The coil-library is the dist...

Tariq Massad, Jüri Jarvet, Risto Tanner, Katrin Tomson, Julia Smirnova, Peep Palumaa, Mariko Sugai, Toshiyuki Kohno, Kalju Vanatalu, Peter Damberg (2007) J Biomol NMR 38: 2 107-123

Positioning of the Alzheimer Abeta(1-40) peptide in SDS micelles using NMR and paramagnetic probes.

2011-05-05T08:40:23Z

NMR spectroscopy combined with paramagnetic relaxation agents was used to study the positioning of the 40-residue Alzheimer Amyloid beta-peptide Abeta(1-40) in SDS micelles. 5-Doxyl stearic acid incorporated into the micelle or Mn(2+) ions in the aqueous solvent were used to determine the position of the peptide relative to the micelle geometry. In SDS solvent, the two alpha-helices induced in Abe...

Jüri Jarvet, Jens Danielsson, Peter Damberg, Marta Oleszczuk, Astrid Gräslund (2007) J Biomol NMR 39: 1 63-72

15N relaxation study of the amyloid beta-peptide: structural propensities and persistence length.

2011-05-05T08:40:23Z

The dynamics of monomeric Alzheimer Abeta1-40 in aqueous solution was studied using heteronuclear NMR experiments. 15N NMR relaxation rates of amide groups report on the dynamics in the peptide chain and make it possible to estimate structural propensities from temperature-dependent relaxation data and chemical shifts change analysis. The persistence length of the polypeptide chain was determined ...

Jens Danielsson, August Andersson, Jüri Jarvet, Astrid Gräslund (2006) Magn Reson Chem 44 Spec No: S114-S121

Characterization of the antioxidative activity of novel nontoxic neuropeptides by using capillary electrophoresis.

2011-05-05T08:40:23Z

In the present study, we have monitored the oxidation process of novel nontoxic neuropeptides and determined its rate constants, which describe the antioxidative potential of the peptides. A capillary electrophoretic method was implemented which ensures the simultaneity of analysis of reactants and products in a short time of analysis. The rate constants of oxidation of the four novel peptides, 4-...

Merike Vaher, Säde Viirlaid, Kersti Ehrlich, Riina Mahlapuu, Jüri Jarvet, Ursel Soomets, Mihkel Kaljurand (2006) Electrophoresis 27: 13 2582-2589

NMR solution structure of the peptide fragment 1-30, derived from unprocessed mouse Doppel protein, in DHPC micelles.

2011-05-05T08:40:23Z

The downstream prion-like Doppel (Dpl) protein is a homologue related to the prion protein (PrP). Dpl is expressed in the brains of mice that do not express PrP, and Dpl is known to be toxic to neurons. One mode of toxicity has been suggested to involve direct membrane interactions. PrP under certain conditions of cell trafficking retains an uncleaved signal peptide, which may also hold for the mu...

Evangelos Papadopoulos, Kamila Oglecka, Lena Mäler, Jüri Jarvet, Peter E Wright, H Jane Dyson, Astrid Gräslund (2006) Biochemistry 45: 1 159-166

Proton magnetic resonance spectroscopy in neuroblastoma: current status, prospects and limitations.

2011-05-05T08:40:23Z

Non-invasive biological information about residual neuroblastoma tumour tissue could allow treatment monitoring without the need for repeated biopsies. Magnetic resonance spectroscopy (MRS) can be performed with standard MR-scanners, providing specific biochemical information from selected tumour regions. By proton 1H-MRS, lipids, certain amino acids and lactate can be detected and their relative ...

Magnus Lindskog, Christian Spenger, Tomas Klason, Jüri Jarvet, Astrid Gräslund, John Inge Johnsen, Frida Ponthan, Lena Douglas, Bo Nordell, Per Kogner (2005) Cancer Lett 228: 1-2 247-255

Limited variations in 15N CSA magnitudes and orientations in ubiquitin are revealed by joint analysis of longitudinal and transverse NMR relaxation.

2011-05-05T08:40:23Z

The site-specific magnitudes and orientations of the chemical shift tensors have been estimated for 70 backbone (15)N-nuclei in human ubiquitin from the field dependence of dynamic independent ratios between relaxation rates, both longitudinal and transverse, measured at 9.4, 11.7, 14.1, and 18.8 T. The results were jointly analyzed with previously published relaxation data [Fushman; Tjandra; Cowb...

Peter Damberg, Jüri Jarvet, Astrid Gräslund (2005) J Am Chem Soc 127: 6 1995-2005

The Alzheimer beta-peptide shows temperature-dependent transitions between left-handed 3-helix, beta-strand and random coil secondary structures.

2011-05-05T08:40:23Z

The temperature-induced structural transitions of the full length Alzheimer amyloid beta-peptide [A(beta)(1-40) peptide] and fragments of it were studied using CD and 1H NMR spectroscopy. The full length peptide undergoes an overall transition from a state with a prominent population of left-handed 3(1) (polyproline II; PII)-helix at 0 degrees C to a random coil state at 60 degrees C, with an aver...

Jens Danielsson, Jüri Jarvet, Peter Damberg, Astrid Gräslund (2005) FEBS J 272: 15 3938-3949

Predicting resistance or response to chemotherapy by proton magnetic resonance spectroscopy in neuroblastoma.

2011-05-05T08:40:46Z

We previously showed that proton magnetic resonance spectroscopy (1H-MRS) enables estimation of neuroblastoma tumor viability. Here we investigated if 1H-MRS can predict response or resistance to chemotherapy in neuroblastoma.

Magnus Lindskog, Christian Spenger, Jüri Jarvet, Astrid Gräslund, Per Kogner (2004) J Natl Cancer Inst 96: 19 1457-1466

Unique physicochemical profile of beta-amyloid peptide variant Abeta1-40E22G protofibrils: conceivable neuropathogen in arctic mutant carriers.

2011-05-05T08:40:46Z

A new early-onset form of Alzheimer's disease (AD) was described recently where a point mutation was discovered in codon 693 of the beta-amyloid (Abeta) precursor protein gene, the Arctic mutation. The mutation translates into a single amino acid substitution, glutamic acid-->glycine, in position 22 of the Abeta peptide. The mutation carriers have lower plasma levels of Abeta than normal, while in...

A Päiviö, J Jarvet, A Gräslund, L Lannfelt, A Westlind-Danielsson (2004) J Mol Biol 339: 1 145-159

Two-site binding of beta-cyclodextrin to the Alzheimer Abeta(1-40) peptide measured with combined PFG-NMR diffusion and induced chemical shifts.

2011-05-05T08:40:46Z

The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of translational diffusion and chemical shift changes are consistent with a model w...

Jens Danielsson, Jüri Jarvet, Peter Damberg, Astrid Gräslund (2004) Biochemistry 43: 20 6261-6269

A left-handed 3(1) helical conformation in the Alzheimer Abeta(12-28) peptide.

2011-05-05T08:40:46Z

We show for the first time that the secondary structure of the Alzheimer beta-peptide is in a temperature-dependent equilibrium between an extended left-handed 3(1) helix and a flexible random coil conformation. Circular dichroism spectra, recorded at 0.03 mM peptide concentration, show that the equilibrium is shifted towards increasing left-handed 3(1) helix structure towards lower temperatures. ...

J Jarvet, P Damberg, J Danielsson, I Johansson, L E G Eriksson, A Gräslund (2003) FEBS Lett 555: 2 371-374

Cell membrane translocation of the N-terminal (1-28) part of the prion protein.

2011-05-05T08:40:46Z

The N-terminal (1-28) part of the mouse prion protein (PrP) is a cell penetrating peptide, capable of transporting large hydrophilic cargoes through a cell membrane. Confocal fluorescence microscopy shows that it transports the protein avidin (67kDa) into several cell lines. The (1-28) peptide has a strong tendency for aggregation and beta-structure formation, particularly in interaction with nega...

P Lundberg, M Magzoub, M Lindberg, M Hällbrink, J Jarvet, L E G Eriksson, U Langel, A Gräslund (2002) Biochem Biophys Res Commun 299: 1 85-90

(13)C-(1)H NMR relaxation and fluorescence anisotropy decay study of tyrosine dynamics in motilin.

2011-05-05T08:40:46Z

Tyrosine ring dynamics of the gastrointestinal hormone motilin was studied using two independent physical methods: fluorescence polarization anisotropy decay and NMR relaxation. Motilin, a 22-residue peptide, was selectively (13)C labeled in the ring epsilon-carbons of the single tyrosine residue. To eliminate effects of differences in peptide concentration, the same motilin sample was used in bot...

Peter Damberg, Jüri Jarvet, Peter Allard, Ulo Mets, Rudolf Rigler, Astrid Gräslund (2002) Biophys J 83: 5 2812-2825

Accurate measurement of translational diffusion coefficients: a practical method to account for nonlinear gradients.

2011-05-05T08:40:46Z

For NMR probes equipped with pulsed field gradient coils, which are not optimized for gradient linearity, the precision and accuracy of experimentally measured translational diffusion coefficients are limited by the linearity of the gradient pulses over the sample volume. This study shows that the accuracy and precision of measured diffusion coefficients by the Stejskal--Tanner spin-echo pulsed fi...

P Damberg, J Jarvet, A Gräslund (2001) J Magn Reson 148: 2 343-348

Secondary structure and position of the cell-penetrating peptide transportan in SDS micelles as determined by NMR.

2011-05-05T08:40:46Z

Transportan is a 27-residue peptide (GWTLN SAGYL LGKIN LKALA ALAKK IL-amide) which has the ability to penetrate into living cells carrying a hydrophilic load. Transportan is a chimeric peptide constructed from the 12 N-terminal residues of galanin in the N-terminus with the 14-residue sequence of mastoparan in the C-terminus and a connecting lysine. Circular dichroism studies of transportan and ma...

M Lindberg, J Jarvet, U Langel, A Gräslund (2001) Biochemistry 40: 10 3141-3149

Micellar systems as solvents in peptide and protein structure determination.

2011-05-05T08:40:46Z

P Damberg, J Jarvet, A Gräslund (2001) Methods Enzymol 339: 271-285

Regulation of GTPase and adenylate cyclase activity by amyloid beta-peptide and its fragments in rat brain tissue.

2011-05-05T08:40:46Z

Modulation of GTPase and adenylate cyclase (ATP pyrophosphate-lyase, EC 4.6.1.1) activity by Alzheimer's disease related amyloid beta-peptide, A beta (1-42), and its shorter fragments, A beta (12-28), A beta (25-35), were studied in isolated membranes from rat ventral hippocampus and frontal cortex. In both tissues, the activity of GTPase and adenylate cyclase was upregulated by A beta (25-35), wh...

U Soomets, R Mahlapuu, R Tehranian, J Jarvet, E Karelson, M Zilmer, K Iverfeldt, M Zorko, A Gräslund, U Langel (1999) Brain Res 850: 1-2 179-188

Structural characterization of inter-alpha-inhibitor. Evidence for an extended shape.

2011-05-05T08:39:47Z

Inter-alpha-inhibitor (IalphaI) is a 180-kDa serum protein consisting of three polypeptides. Two of these, the heavy chains 1 and 2 (H1 and H2), are of 75-80 kDa and have similar amino acid sequences. The third polypeptide, bikunin, has a molecular mass of 25 kDa and contains a 7-kDa chondroitin sulfate chain that is covalently linked to the C-terminal amino acid residues of H1 and H2. IalphaI has...

A M Blom, M Mörgelin, M Oyen, J Jarvet, E Fries (1999) J Biol Chem 274: 1 298-304

Quantitative estimation of magnitude and orientation of the CSA tensor from field dependence of longitudinal NMR relaxation rates.

2011-05-05T08:39:47Z

A method is presented that makes it possible to estimate both the orientation and the magnitude of the chemical shift anisotropy (CSA) tensor in molecules with a pair of spin 1/2 nuclei, typically (13)C-(1)H or (15) N-(1)H. The method relies on the fact that the longitudinal cross-correlation rate as well as a linear combination of the autorelaxation rates of longitudinal heterospin magnetization,...

P Damberg, J Jarvet, P Allard, A Gräslund (1999) J Biomol NMR 15: 1 27-37

Three-dimensional structure and position of porcine motilin in sodium dodecyl sulfate micelles determined by 1H NMR.

2011-05-05T08:39:47Z

The solution structure of the porcine gastrointestinal peptide hormone motilin was determined in the presence of sodium dodecyl sulfate (SDS) micelles at 28 degrees C using 1H nuclear magnetic resonance, full relaxation matrix analysis, and structure calculations based on restrained molecular dynamics. The structure of motilin in SDS micelles is described by a reverse gamma-turn and a beta-turn of...

J Jarvet, J Zdunek, P Damberg, A Gräslund (1997) Biochemistry 36: 26 8153-8163

Phase-Sensitive Two-Dimensional Heteronuclear Zero- and Double-Quantum-Coherence Spectroscopy

2011-05-05T08:39:47Z

New pulse sequences for two-dimensional phase-sensitive detection of heteronuclear zero- and double-quantum coherence are presented for use with and without pulsed field gradients. The magnetization is phase modulated during t1 and then transferred to a proton for detection using a novel approach similar to the sensitivity-enhancement technique [Palmer et al. J. Magn. Reson. 93, 151 (1991)]. These...

Jarvet, Allard (1996) J Magn Reson B 112: 3 240-244

Spectral-Density Mapping of 13Calpha-1Halpha Vector Dynamics Using Dipolar Relaxation Rates Measured at Several Magnetic Fields

2011-05-05T08:39:47Z

The spectral-density mapping of a 13Calpha-1Halpha vector of Leu10 in the 22-residue peptide hormone motilin [P. Allard, J. Jarvet, A. Ehrenberg, and A. Graslund, J. Biomol. NMR 5, 133-146 (1995)] is extended in this paper to three polarizing fields 9.4, 11.7, and 14.1 T in order to improve the accuracy of the calculated spectral-density function J(omega) and to extend the sampling range up to 750...

Jarvet, Allard, Ehrenberg, Graslund (1996) J Magn Reson B 111: 1 23-30

Mapping of the spectral density function of a C alpha-H alpha bond vector from NMR relaxation rates of a 13C-labelled alpha-carbon in motilin.

2011-05-05T08:39:47Z

The peptide hormone motilin was synthesised with a 13C-enriched alpha-carbon in the leucine at position 10. In aqueous solution, six different relaxation rates were measured for the 13C alpha-H alpha fragment as a function of temperature and with and without the addition of 30% (v/v) of the cosolvent d2-1,1,1,3,3,3-hexafluoro-2-propanol (HFP). The relaxation rates were analysed employing the spect...

P Allard, J Jarvet, A Ehrenberg, A Gräslund (1995) J Biomol NMR 5: 2 133-146

Solution structure by 1H and dynamics by natural abundance 13C NMR of a receptor recognising peptide derived from a C-terminal fragment of neuropeptide Y.

2011-05-05T08:39:47Z

A peptide consisting of 20 amino acid residues, derived from a C-terminal fragment of neuropeptide Y (NPY) and showing high affinity to NPY receptors, was synthesised. Its sequence is PAADLARYRHYINLITRQRY-NH2, and the solution structure was calculated from NMR-derived distance and torsion angle restraints, obtained at 15 degrees C in a solvent mixture of water and 30% (v/v) 1,1,1,3,3,3-hexafluoro-...

K Arvidsson, J Jarvet, P Allard, A Ehrenberg (1994) J Biomol NMR 4: 5 653-672

13C and 15N NMR and time-resolved fluorescence depolarization study of bovine carbonic anhydrase--4-methylbenzenesulfonamide complex.

2011-05-05T08:39:47Z

The influence of the binding of the high-affinity inhibitor, 4-methylbenzenesulfonamide, to the active site of bovine carbonic anhydrase B was studied by 15N- and 13C-NMR spectroscopy. The rotational correlation time dependence on temperature and concentration of the complex was determined by time-resolved fluorescence depolarization measurements. Our experiment provides evidence that the stoichio...

J Jarvet, A Olivson, U Mets, M Pooga, R Aguraiuja, E Lippmaa (1989) Eur J Biochem 186: 1-2 287-290