gloux karine's Publications List

Microbes and Health Sackler Colloquium: A metagenomic {beta}-glucuronidase uncovers a core adaptive function of the human intestinal microbiome.

2011-03-16T08:57:09Z

In the human gastrointestinal tract, bacterial beta-D-glucuronidases (BG; E.C. 3.2.1.31) are involved both in xenobiotic metabolism and in some of the beneficial effects of dietary compounds. Despite their biological significance, investigations are hampered by the fact that only a few BGs have so far been studied. A functional metagenomic approach was therefore performed on intestinal metagenomic...

Karine Gloux, Olivier Berteau, Hanane El Oumami, Fabienne Béguet, Marion Leclerc, Joël Doré (2010) Proc Natl Acad Sci U S A :

Functional metagenomics: a high throughput screening method to decipher microbiota-driven NF-κB modulation in the human gut.

2011-03-16T08:57:09Z

The human intestinal microbiota plays an important role in modulation of mucosal immune responses. To study interactions between intestinal epithelial cells (IECs) and commensal bacteria, a functional metagenomic approach was developed. One interest of metagenomics is to provide access to genomes of uncultured microbes. We aimed at identifying bacterial genes involved in regulation of NF-κB signa...

Omar Lakhdari, Antonietta Cultrone, Julien Tap, Karine Gloux, Françoise Bernard, S Dusko Ehrlich, Fabrice Lefèvre, Joël Doré, Hervé M Blottière (2010) PLoS One 5: 9

A comparison of random sequence reads versus 16S rDNA sequences for estimating the biodiversity of a metagenomic library.

2011-03-16T08:57:09Z

The construction of metagenomic libraries has permitted the study of microorganisms resistant to isolation and the analysis of 16S rDNA sequences has been used for over two decades to examine bacterial biodiversity. Here, we show that the analysis of random sequence reads (RSRs) instead of 16S is a suitable shortcut to estimate the biodiversity of a bacterial community from metagenomic libraries. ...

Chaysavanh Manichanh, Charles E Chapple, Lionel Frangeul, Karine Gloux, Roderic Guigo, Joel Dore (2008) Nucleic Acids Res 36: 16 5180-5188

Development of high-throughput phenotyping of metagenomic clones from the human gut microbiome for modulation of eukaryotic cell growth.

2011-03-16T08:57:09Z

Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.

Karine Gloux, Marion Leclerc, Harout Iliozer, René L'Haridon, Chaysavanh Manichanh, Gérard Corthier, Renaud Nalin, Hervé M Blottière, Joël Doré (2007) Appl Environ Microbiol 73: 11 3734-3737

Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach.

2011-03-16T08:57:09Z

A role for the intestinal microbial community (microbiota) in the onset and chronicity of Crohn's disease (CD) is strongly suspected. However, investigation of such a complex ecosystem is difficult, even with culture independent molecular approaches.

C Manichanh, L Rigottier-Gois, E Bonnaud, K Gloux, E Pelletier, L Frangeul, R Nalin, C Jarrin, P Chardon, P Marteau, J Roca, J Dore (2006) Gut 55: 2 205-211

Mutations of ousA alter the virulence of Erwinia chrysanthemi.

2011-03-16T08:57:09Z

A negative correlation was observed between the aggressiveness of several Erwinia chrysanthemi strains on potato tuber and their osmotic tolerance. The disruption of the ousA gene encoding the major osmoprotectant uptake system highly enhanced bacterial virulence on potato tubers. The ousA disruption also increased the maceration efficiency on potato tubers under anaerobic conditions. In the absen...

Karine Gloux, Thierry Touze, Yves Pagot, Bernard Jouan, Carlos Blanco (2005) Mol Plant Microbe Interact 18: 2 150-157

Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: role of xenobiotic metabolising enzymes and intestinal microflora.

2011-03-16T08:57:09Z

We investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes ...

Christèle Humblot, Evelyne Lhoste, Siegfried Knasmüller, Karine Gloux, Aurélia Bruneau, Martine Bensaada, José Durao, Sylvie Rabot, Claude Andrieux, Fekadu Kassie (2004) J Chromatogr B Analyt Technol Biomed Life Sci 802: 1 231-237

The activities of several detoxication enzymes are differentially induced by juices of garden cress, water cress and mustard in human HepG2 cells.

2011-03-16T08:57:09Z

It has been previously demonstrated in a human-derived hepatoma cell line (HepG2) that juices from cruciferous vegetables protect against the genotoxicity caused by dietary carcinogens. HepG2 cells possess different enzymes involved in the biotransformation of xenobiotics. Therefore, we investigated the effect of cruciferous juices on the activities of CYP 1A and several phase II enzymes in this c...

E F Lhoste, K Gloux, I De Waziers, S Garrido, S Lory, C Philippe, S Rabot, S Knasmüller (2004) Chem Biol Interact 150: 3 211-219

Osmoprotection of Escherichia coli by ectoine: uptake and accumulation characteristics.

2011-03-16T08:56:40Z

Ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) is a cyclic amino acid, identified as a compatible solute in moderately halophilic bacteria. Exogenously provided ectoine was found to stimulate growth of Escherichia coli in media of inhibitory osmotic strength. The stimulation was independent of any specific solute, electrolyte or nonelectrolyte. It is accumulated in E. coli cells...

M Jebbar, R Talibart, K Gloux, T Bernard, C Blanco (1992) J Bacteriol 174: 15 5027-5035

Identification of an osmotically induced periplasmic glycine betaine-binding protein from Rhizobium meliloti.

2011-03-16T08:56:40Z

The effect of salt stress on glycine betaine-binding activity has been investigated in periplasmic fractions released from Rhizobium meliloti 102F34 by cold osmotic shock. Binding activity was monitored by three techniques: equilibrium dialysis, filter procedure, and detection of 14C ligand-protein binding by direct non-denaturing polyacrylamide gel electrophoresis (PAGE) followed by autoradiograp...

D Le Rudulier, K Gloux, N Riou (1991) Biochim Biophys Acta 1061: 2 197-205