http://publicationslist.org/data/marco.paggi/atom.xml Marco Paggihttp://publicationslist.org/marco.paggi$basepathfavicon.icoRecent additions to Marco Paggi's PublicationsList.org pagehttp://publicationslist.org/publications.png2008-04-10T16:18:57Z http://publicationslist.org/marco.paggi/refid1 2008-04-10T16:16:42Z

A strategy for the selective in vivo activation of prodrugs by proteases is presented. The approach is based on the design of polythiol peptides able to neutralize the toxicity of As(III) through chelation, and contemporarily to be recognized as substrates of a disease-linked specific protease. Enzyme digestion implies conversion of such polythiol peptides into monothiol fragments with irreversibl...<br/><br/>Emanuele Bellacchio, Marco G Paggi (2008) <i>J Cell Physiol</i> <i></i> <i></i> 214: 3 681-686<br/>

http://publicationslist.org/marco.paggi/refid5 2008-04-10T16:16:42Z

We investigated several phenotypic and functional parameters of T cell-mediated immunity in a large series of common variable immunodeficiency (CVID) patients. We demonstrated that the vast majority of CVID patients presented multiple T cell abnormalities intimately related among them, the severity of which was reflected in a parallel loss of CD4+ naive T cells. A strong correlation between the nu...<br/><br/>Antonello Giovannetti, Marina Pierdominici, Francesca Mazzetta, Marco Marziali, Cristina Renzi, Anna Maria Mileo, Marco De Felice, Barbara Mora, Antonella Esposito, Rossella Carello, Antonio Pizzuti, Marco G Paggi, Roberto Paganelli, Walter Malorni, Fernando Aiuti (2007) <i>J Immunol</i> <i></i> <i></i> 178: 6 3932-3943<br/>

http://publicationslist.org/marco.paggi/refid7 2008-04-10T16:16:42Z

One strategy in the development of anticancer therapeutics has been to arrest malignant proliferation through inhibition of the enzymatic activity of cyclin-dependent kinases (cdks), which are key regulatory molecules of the cell cycle. Over the past few years, numerous compounds with remarkable cdk inhibitory activity have been studied in cancer therapy, although it is very difficult to point out...<br/><br/>L Bagella, A Sun, T Tonini, G Abbadessa, G Cottone, M G Paggi, A De Luca, P P Claudio, A Giordano (2007) <i>Oncogene</i> <i></i> <i></i> 26: 13 1829-1839<br/>

http://publicationslist.org/marco.paggi/refid4 2008-04-10T16:16:42Z

The viral factor E7 plays a key role in the well-established association between "high-risk" Human Papillomavirus (HPV) infection and the development of epithelial malignant tumors, as uterine cervix and ano-genital cancer. To delve into the molecular mechanisms of HPV-mediated cell transformation, we searched for novel potential cellular targets of the HPV-16 E7 oncoprotein, by means of the yeast...<br/><br/>Anna Severino, Claudia Abbruzzese, Lucrezia Manente, Alvaro Avivar Valderas, Stefano Mattarocci, Antonio Federico, Giuseppe Starace, Alberto Chersi, Anna Maria Mileo, Marco G Paggi (2007) <i>J Cell Physiol</i> <i></i> <i></i> 212: 1 118-125<br/>

http://publicationslist.org/marco.paggi/refid3 2008-04-10T16:16:42Z

The amount of information on tumor biology has expanded enormously, essentially due to the completion of the human genome sequencing and to the application of new technologies that represent an exciting breakthrough in molecular analysis. Often these data spring from experimental procedures, such as a serial analysis of gene expression (SAGE) and DNA microarrays, which cannot be defined as hypothe...<br/><br/>Armando Felsani, Anna Maria Mileo, Vittoria Maresca, Mauro Picardo, Marco G Paggi (2007) <i>Int Rev Cytol</i> <i></i> <i></i> 261: 247-286<br/>

http://publicationslist.org/marco.paggi/refid2 2008-04-10T16:16:42Z

Retinoblastoma (RB) family proteins pRb, p107 and pRb2/p130 are important cellular factors which play a well-recognized role as tumor and growth suppressors. These proteins are actively involved in the negative control of the cell cycle and their function is modulated via complex homeostatic processes, most of them involving post-translational regulation of their phosphorylation status. Interestin...<br/><br/>Antonio Giordano, Emanuele Bellacchio, Luigi Bagella, Marco G Paggi (2007) <i>Cell Cycle</i> <i></i> <i></i> 6: 21 2591-2593<br/>

http://publicationslist.org/marco.paggi/refid9 2008-04-10T16:16:42Z

With the aim to find novel partners of human Cyclin T2a, we performed a two-hybrid screening in yeast using the full-length cDNA of this cyclin as bait, and a human heart cDNA library as preys source. Upon several interesting genes selected, our attention has been focused on the cDNA coding for PKNalpha, a fatty acid- and Rho-activated serine/threonine protein kinase, having a catalytic domain hom...<br/><br/>Giuliano Cottone, Alfonso Baldi, Emanuele Palescandolo, Lucrezia Manente, Roberta Penta, Marco G Paggi, Antonio De Luca (2006) <i>J Cell Physiol</i> <i></i> <i></i> 207: 1 232-237<br/>

http://publicationslist.org/marco.paggi/refid8 2008-04-10T16:16:42Z

RB, the most investigated tumor suppressor gene, is the founder of the RB family of growth/tumor suppressors, which comprises also p107 (RBL1) and Rb2/p130 (RBL2). The protein products of these genes, pRb, p107 and pRb2/p130, respectively, are also known as 'pocket proteins', because they share a 'pocket' domain responsible for most of the functional interactions characterizing the activity of thi...<br/><br/>A Felsani, A M Mileo, M G Paggi (2006) <i>Oncogene</i> <i></i> <i></i> 25: 38 5277-5285<br/>

http://publicationslist.org/marco.paggi/refid10 2008-04-10T16:16:42Z

Recently, after the identification of ferritin light chain (L-ferritin) gene and protein over-expression in human metastatic melanoma cells, we engineered, starting from the LM metastatic melanoma cell line, clones in which L-ferritin gene expression was down-regulated by the stable expression of a specific antisense construct. The present investigation started from the observation that L-ferritin...<br/><br/>Vittoria Maresca, Enrica Flori, Giorgia Cardinali, Stefania Briganti, Daniela Lombardi, Anna Maria Mileo, Marco G Paggi, Mauro Picardo (2006) <i>J Cell Physiol</i> <i></i> <i></i> 206: 3 843-848<br/>

http://publicationslist.org/marco.paggi/refid6 2008-04-10T16:16:42Z

High-risk human papillomaviruses (HPV) are linked to human cervical and other ano-genital cancers. Integration of the viral genome in the transformed epithelial cells is restricted to the coding regions for the E6 and E7 oncoproteins. Nevertheless, E7 plays the major role in cell transformation. We report a novel interaction between HPV-16 E7 and the Nm23-H1 and Nm23-H2 proteins identified in yeas...<br/><br/>Anna Maria Mileo, Emanuela Piombino, Anna Severino, Alessandra Tritarelli, Marco G Paggi, Daniela Lombardi (2006) <i>J Bioenerg Biomembr</i> <i></i> <i></i> 38: 3-4 215-225<br/>

http://publicationslist.org/marco.paggi/refid13 2008-04-10T16:16:42Z

The cell cycle regulatory pathway responsible for the control of the late-G1 checkpoint is found recurrently altered in human malignant melanoma, often due to lack of functional p16 or pRb (pRb-1) proteins. Here we examined the ability of p16-derived peptides to mimic p16 function in two exemplary human melanoma cell lines: the p16-defective, pRb-positive A375M cells and p16-positive, pRb-defectiv...<br/><br/>Douglas M Noonan, Anna Severino, Monica Morini, Alessandra Tritarelli, Lucrezia Manente, Igea D'Agnano, Giuseppe Starace, Alfonso Baldi, Daniela Lombardi, Adriana Albini, Armando Felsani, Marco G Paggi (2005) <i>J Cell Physiol</i> <i></i> <i></i> 202: 3 922-928<br/>

http://publicationslist.org/marco.paggi/refid11 2008-04-10T16:16:42Z

PURPOSE: Employing an in vitro model system of human melanoma progression, we previously reported ferritin light chain (L-ferritin) gene overexpression in the metastatic phenotype. Here, we attempted to characterize the role of ferritin in the biology of human melanoma and in the progression of this disease. EXPERIMENTAL DESIGN: Starting from the LM human metastatic melanoma cell line, we engineer...<br/><br/>Alfonso Baldi, Daniela Lombardi, Patrizia Russo, Emanuele Palescandolo, Antonio De Luca, Daniele Santini, Feliciano Baldi, Luigi Rossiello, Maria Lucia Dell'Anna, Arianna Mastrofrancesco, Vittoria Maresca, Enrica Flori, Pier Giorgio Natali, Mauro Picardo, Marco G Paggi (2005) <i>Clin Cancer Res</i> <i></i> <i></i> 11: 9 3175-3183<br/>

http://publicationslist.org/marco.paggi/refid17 2008-04-10T16:16:42Z

The adenoviral E1A proteins have been implicated in promotion of proliferation and transformation, inhibition of differentiation, induction of apoptosis, regulation of transcription, and suppression of tumor growth. The ability of E1A to override the fundamental controls of host cells is based on its ability to physically interact with several cellular proteins. We recently characterized RACK1 as ...<br/><br/>Anna Severino, Alfonso Baldi, Giuliano Cottone, Mei Han, Nianli Sang, Antonio Giordano, Anna Maria Mileo, Marco G Paggi, Antonio De Luca (2004) <i>J Cell Physiol</i> <i></i> <i></i> 199: 1 134-139<br/>

http://publicationslist.org/marco.paggi/refid16 2008-04-10T16:16:42Z

APAF-1 plays a pivotal role in mitochondria-dependent apoptosis, binding to cytochrome c and favoring activation of caspase-9. It has been shown that epigenetic silencing of the APAF-1 gene is a common event in several metastatic melanoma cells in vitro. We determined, by Western blot, variation in the level of expression of APAF-1 in several human melanoma cell lines and, by immunohistochemistry,...<br/><br/>Alfonso Baldi, Daniele Santini, Patrizia Russo, Caterina Catricalà, Ada Amantea, Mauro Picardo, Fabiana Tatangelo, Gerardo Botti, Emanuele Dragonetti, Raffaele Murace, Giuseppe Tonini, Pier Giorgio Natali, Feliciano Baldi, Marco G Paggi (2004) <i>Exp Dermatol</i> <i></i> <i></i> 13: 2 93-97<br/>

http://publicationslist.org/marco.paggi/refid15 2008-04-10T16:16:42Z

Signaling through the Notch cell surface receptors is a highly conserved mechanism of cell fate specification. Notch signaling regulates proliferation, differentiation and cell death. In vertebrates, putative gene duplication has originated four Notch genes, Notch-1, -2, -3 and -4. They have been implicated in neurogenesis, hematopoiesis, T-cell development, vasculogenesis and brain cortical growt...<br/><br/>Alfonso Baldi, Maria De Falco, Luca De Luca, Giuliano Cottone, Marco G Paggi, Brian J Nickoloff, Lucio Miele, Antonio De Luca (2004) <i>Biol Cell</i> <i></i> <i></i> 96: 4 303-311<br/>

http://publicationslist.org/marco.paggi/refid14 2008-04-10T16:16:42Z

The placenta has a dynamic and continuous capacity for self-renewal. The molecular mechanisms responsible for controlling trophoblast proliferation are still unclear. It is generally accepted that the simultaneous activity of proteins involved in cell proliferation, apoptosis, and extracellular matrix degradation plays an important role in correct placental development. We investigated in depth th...<br/><br/>Antonio De Luca, Maria De Falco, Valentina Fedele, Luigi Cobellis, Annunziata Mastrogiacomo, Vincenza Laforgia, Ioana L Tuduce, Mara Campioni, Domenico Giraldi, Marco G Paggi, Alfonso Baldi (2004) <i>J Histochem Cytochem</i> <i></i> <i></i> 52: 7 885-892<br/>

http://publicationslist.org/marco.paggi/refid12 2008-04-10T16:16:42Z

The human HtrA family of proteases consists of four members: HtrA1, HtrA2, HtrA3, and HtrA4. In humans the four HtrA homologues appear to be involved in several important functions such as cell growth, apoptosis, and inflammatory reactions, and they control cell fate via regulated protein metabolism. In previous studies it was shown that the expression of HtrA1 was ubiquitous in normal adult human...<br/><br/>Antonio De Luca, Maria De Falco, Luca De Luca, Roberta Penta, Viji Shridhar, Feliciano Baldi, Mara Campioni, Marco G Paggi, Alfonso Baldi (2004) <i>J Histochem Cytochem</i> <i></i> <i></i> 52: 12 1609-1617<br/>

http://publicationslist.org/marco.paggi/refid24 2008-04-10T16:16:42Z

In order to identify genes relevant for melanoma development, we carried out cDNA array experiments employing an in vitro model of human melanoma progression, consisting of two cell lines: one, LP, derived from a primary melanoma and the other, LM, from its metastatic supraclavicular lymph node. Basic cDNA array data identified 26 genes as down-regulated in the LM cell line. Northern blot analysis...<br/><br/>Alfonso Baldi, Tullio Battista, Antonio De Luca, Daniele Santini, Luigi Rossiello, Feliciano Baldi, Pier Giorgio Natali, Daniela Lombardi, Mauro Picardo, Armando Felsani, Marco G Paggi (2003) <i>Exp Dermatol</i> <i></i> <i></i> 12: 2 213-218<br/>

http://publicationslist.org/marco.paggi/refid23 2008-04-10T16:16:42Z

Marco G Paggi, Armando Felsani, Antonio Giordano (2003) <i>Methods Mol Biol</i> <i></i> <i></i> 222: 3-19<br/>

http://publicationslist.org/marco.paggi/refid26 2008-04-10T16:16:42Z

Cyclins are members of family of proteins involved in the cell cycle regulation. They are regulatory subunits of complexes with proteins called cyclin-dependent kinases (CDKs). There are three forms of cyclin T: cyclin T1, cyclin T2a, and T2b. All cyclin T contain an N-terminal "cyclin homology box," the most conserved region among different members of the cyclin family that serves to bind CDK9. I...<br/><br/>Antonio De Luca, Maria De Falco, Alfonso Baldi, Marco G Paggi (2003) <i>J Cell Physiol</i> <i></i> <i></i> 194: 2 101-107<br/>

http://publicationslist.org/marco.paggi/refid22 2008-04-10T16:16:42Z

Genetic aberrations, mostly resulting in changes in gene expression, are critical events in cancer onset and progression. The advent of the cDNA array technology allows the screening and the efficient measurement of expression of thousands genes simultaneously in a wide spectrum of experimental and clinical models. This genomic scale approach is being currently used to obtain global views of human...<br/><br/>Alfonso Baldi, Daniele Santini, Antonio De Luca, Marco G Paggi (2003) <i>J Cell Physiol</i> <i></i> <i></i> 196: 2 219-223<br/>

http://publicationslist.org/marco.paggi/refid25 2008-04-10T16:16:42Z

By means of the yeast two-hybrid system, we have discovered a novel physical interaction between the adenovirus E1A oncoprotein and Ran, a small GTPase which regulates nucleocytoplasmic transport, cell cycle progression, and mitotic spindle organization. Expression of E1A elicits induction of S phase and centrosome amplification in a variety of rodent cell lines. The induction of supernumerary cen...<br/><br/>Antonio De Luca, Rosamaria Mangiacasale, Anna Severino, Lorenzo Malquori, Alfonso Baldi, Antonella Palena, Anna Maria Mileo, Patrizia Lavia, Marco G Paggi (2003) <i>Cancer Res</i> <i></i> <i></i> 63: 6 1430-1437<br/>

http://publicationslist.org/marco.paggi/refid20 2008-04-10T16:16:42Z

Despite the clonal origin of most tumors, their tremendous heterogeneity suggests that cancer progression springs from the combined forces of both genetic and epigenetic events, which produce variant clonal populations, together with the selective pressures of the microenvironment, which promote growth and, perhaps, dissemination of variants with a specific set of characteristics. Although the imp...<br/><br/>Marcella Macaluso, Marco G Paggi, Antonio Giordano (2003) <i>Oncogene</i> <i></i> <i></i> 22: 42 6472-6478<br/>

http://publicationslist.org/marco.paggi/refid18 2008-04-10T16:16:42Z

Adenocarcinoma of the stomach is the second most common cause of cancer mortality in the world. The purpose of this study was to evaluate the potential role in carcinogenesis of two secreted Helicobacter pylori's proteins, CagA and HspB, both shown to increase the risk of gastric carcinoma in patients infected with H. pylori-positive strain. The effects of these two proteins on cell kinetics and t...<br/><br/>Antonio De Luca, Alfonso Baldi, Patrizia Russo, Anna Todisco, Lucia Altucci, Nicola Giardullo, Luigi Pasquale, Salvatore Iaquinto, Vittorio D'Onofrio, Maria Caterina Parodi, Marco G Paggi, Gaetano Iaquinto (2003) <i>Cancer Res</i> <i></i> <i></i> 63: 19 6350-6356<br/>

http://publicationslist.org/marco.paggi/refid19 2008-04-10T16:16:42Z

The small DNA virus proteins E1A and E1B from human Adenovirus, E6 and E7 from human papillomavirus, and large T and small T antigens from SV40, are multifaceted molecular tools that can carry out an impressive number of tasks in the host cell. These viral factors, collectively termed 'oncoproteins' for their ability to induce cancer, can be viewed as paradigmatic oncogenic factors which can disru...<br/><br/>Patrizia Lavia, Anna Maria Mileo, Antonio Giordano, Marco G Paggi (2003) <i>Oncogene</i> <i></i> <i></i> 22: 42 6508-6516<br/>

http://publicationslist.org/marco.paggi/refid21 2008-04-10T16:16:42Z

The human HtrA family of proteases consists of three members: HtrA1, HtrA2, and HtrA3. In bacteria, the chief role of HtrA is recognition and degradation of misfolded proteins in the periplasm, combining a dual activity of chaperone and protease. In humans, the three HtrA homologues appear to be involved in diverse functions such as cell growth, apoptosis, allergic reactions, fertilization, contro...<br/><br/>Antonio De Luca, Maria De Falco, Anna Severino, Mara Campioni, Daniele Santini, Feliciano Baldi, Marco G Paggi, Alfonso Baldi (2003) <i>J Histochem Cytochem</i> <i></i> <i></i> 51: 10 1279-1284<br/>

http://publicationslist.org/marco.paggi/refid28 2008-04-10T16:16:42Z

BACKGROUND: Mesothelioma is the most commonly occurring primary pleural neoplasm. Several studies have documented an increase in the incidence of this malignancy during the last decades. Although the association between asbestos exposure and development of mesothelioma is generally accepted, the exact mechanism of carcinogenesis is unknown. Recently, Simian virus 40 large T antigen (SV40 Tag) expr...<br/><br/>A Baldi, A M Groeger, V Esposito, R Cassandro, G Tonini, T Battista, M P Di Marino, B Vincenzi, M Santini, A Angelini, R Rossiello, F Baldi, M G Paggi (2002) <i>Thorax</i> <i></i> <i></i> 57: 4 353-356<br/>

http://publicationslist.org/marco.paggi/refid27 2008-04-10T16:16:42Z

Differential gene expression of cell lines derived from a malignant melanoma or its autologous lymph node metastasis using cDNA arrays indicated down-regulation of PRSS11, a gene encoding the serine protease HtrA1, a homolog of the Escherichia coli protease HtrA, in the metastatic line. Stable PRSS11 overexpression in the metastatic cell line strongly inhibited proliferation, chemoinvasion and Nm2...<br/><br/>Alfonso Baldi, Antonio De Luca, Monica Morini, Tullio Battista, Armando Felsani, Feliciano Baldi, Caterina Catricalà, Ada Amantea, Douglas M Noonan, Adriana Albini, Pier Giorgio Natali, Daniela Lombardi, Marco G Paggi (2002) <i>Oncogene</i> <i></i> <i></i> 21: 43 6684-6688<br/>

http://publicationslist.org/marco.paggi/refid34 2008-04-10T16:16:42Z

A Baldi, D Santini, M G Paggi (2001) <i>J Cell Physiol</i> <i></i> <i></i> 188: 1 139-142<br/>

http://publicationslist.org/marco.paggi/refid37 2008-04-10T16:16:42Z

The adenoviral E1A proteins are able to promote proliferation and transformation, inhibit differentiation, induce apoptosis, and suppress tumor growth. The extreme N terminus and conserved region one of E1A, which are indispensable for transcriptional regulation and for binding to p300/CBP, TBP, and pCAF, play essential roles in these abilities. These observations strongly suggest an intrinsic lin...<br/><br/>N Sang, A Severino, P Russo, A Baldi, A Giordano, A M Mileo, M G Paggi, A De Luca (2001) <i>J Biol Chem</i> <i></i> <i></i> 276: 29 27026-27033<br/>

http://publicationslist.org/marco.paggi/refid35 2008-04-10T16:16:42Z

Cyclin T2a was recently identified as one of the regulatory subunits of the cdk-cyclin complex P-TEFb, the most studied positive factor in the regulation of transcription elongation. By fluorescent in situ hybridization (FISH), the gene codifying for cyclin T2a has been mapped on human chromosome 2q21. This locus also has been linked to different forms of myopathy. By use of a new specific antiser...<br/><br/>A De Luca, A Tosolini, P Russo, A Severino, A Baldi, L De Luca, I Cavallotti, F Baldi, A Giordano, J R Testa, M G Paggi (2001) <i>J Histochem Cytochem</i> <i></i> <i></i> 49: 6 693-698<br/>

http://publicationslist.org/marco.paggi/refid36 2008-04-10T16:16:42Z

Cyclin T1 was recently identified, together with cdk9 (previously named PITALRE), as part of the TAK multiprotein complex, a co-factor targeted by the human immunodeficiency virus Type 1 (HIV-1) protein named Tat, suggesting a role for this complex in transcription elongation. Although studies on mRNA and protein expression have shown that cyclin T1 is ubiquitous in adult human tissues, no data ha...<br/><br/>A De Luca, P Russo, A Severino, A Baldi, T Battista, I Cavallotti, L De Luca, F Baldi, A Giordano, M G Paggi (2001) <i>J Histochem Cytochem</i> <i></i> <i></i> 49: 6 685-692<br/>

http://publicationslist.org/marco.paggi/refid33 2008-04-10T16:16:42Z

This review portrays an updated overview about the possible tumor suppressive properties of the Rb2/p130 gene, the third member of the retinoblastoma (RB) family of genes, including RB itself and p107. After a brief analysis of the established structural and functional similarities among the three genes, the main purpose is to critically analyze present evidence whether Rb2/p130 shares the role of...<br/><br/>M G Paggi, A Giordano (2001) <i>Cancer Res</i> <i></i> <i></i> 61: 12 4651-4654<br/>

http://publicationslist.org/marco.paggi/refid31 2008-04-10T16:16:42Z

Increasing evidence indicates that the nm23 genes, initially documented as suppressors of metastasis progression, are involved in normal development and differentiation. We have shown previously that the murine nm23 gene enhances pheochromocytoma PC12 cells responsiveness to NGF by accelerating cell growth arrest and neurite outgrowth. The present study was aimed at elucidating the mechanisms by w...<br/><br/>D Lombardi, E Palescandolo, A Giordano, M G Paggi (2001) <i>Cell Death Differ</i> <i></i> <i></i> 8: 5 470-476<br/>

http://publicationslist.org/marco.paggi/refid32 2008-04-10T16:16:42Z

Rb2/p130, a member of the Retinoblastoma family of growth and tumour suppressor genes, is extensively implicated in the control of cell cycle and differentiation. The minimal promoter region of Rb2/p130 in T98G human glioblastoma cells was identified and its analysis revealed the presence of a KER1 palindromic sequence able to bind the transcription factor AP-2, a regulatory protein that plays a c...<br/><br/>M G Paggi, F Bonetto, A Severino, A Baldi, T Battista, F Bucci, A Felsani, D Lombardi, A Giordano (2001) <i>Oncogene</i> <i></i> <i></i> 20: 20 2570-2578<br/>

http://publicationslist.org/marco.paggi/refid29 2008-04-10T16:16:42Z

The AP-2 transcription factor plays a pivotal role in regulating the expression of several genes involved in tumor growth and progression of melanoma. We determined, by Western blot, variation in the level of expression of AP-2 and three of its downstream targets, c-kit, E-cadherin, and p21 in several human melanoma cell lines and, by immunohistochemistry, in a group of 99 histological samples inc...<br/><br/>A Baldi, D Santini, T Battista, E Dragonetti, G Ferranti, T Petitti, A M Groeger, A Angelini, R Rossiello, F Baldi, P G Natali, M G Paggi (2001) <i>J Cell Biochem</i> <i></i> <i></i> 83: 3 364-372<br/>

http://publicationslist.org/marco.paggi/refid30 2008-04-10T16:16:42Z

BACKGROUND: The expression of several genes is modulated during neuroblastoma differentiation. The retinoblastoma family proteins, pRb, p107 and pRb2/p130, act in the repression of proliferation genes, interacting mainly with the E2F transcription factors. PROCEDURE AND RESULTS: In this study, we found that, in neuroblastoma cell lines, pRb and p107 proteins decreased, undergoing progressive depho...<br/><br/>G Raschellà, B Tanno, F Bonetto, A Negroni, R Amendola, M G Paggi (2001) <i>Med Pediatr Oncol</i> <i></i> <i></i> 36: 1 104-107<br/>

http://publicationslist.org/marco.paggi/refid39 2008-04-10T16:16:42Z

Tumor suppressor genes have a pivotal role in normal cells regulating cell cycle processes negatively. Furthermore, the inhibition of cell proliferation is a crucial step in the achievement of cell differentiation. Increasing evidence suggests that the nm23 genes, initially documented as suppressors of the invasive phenotype in some cancer types, are involved in the control of normal development a...<br/><br/>D Lombardi, M L Lacombe, M G Paggi (2000) <i>J Cell Physiol</i> <i></i> <i></i> 182: 2 144-149<br/>

http://publicationslist.org/marco.paggi/refid38 2008-04-10T16:16:42Z

The p53 protein accumulates rapidly through post-transcriptional mechanisms following cellular exposure to DNA damaging agents and is also activated as a transcription factor leading to growth arrest or apoptosis. Phosphorylation of p53 occurs after DNA damage thereby modulating its activity and impeding the interaction of p53 with its negative regulator oncogene Mdm2. The serines 15 and 37 presen...<br/><br/>C Cinti, P P Claudio, A D Luca, M Cuccurese, C M Howard, M D'Esposito, M G Paggi, D L Sala, L Azzoni, T D Halazonetis, A Giordano, N M Maraldi (2000) <i>Oncogene</i> <i></i> <i></i> 19: 44 5098-5105<br/>

http://publicationslist.org/marco.paggi/refid40 2008-04-10T16:16:42Z

An association between cyclin D3 and the C-terminal domain of pRb2/p130 was demonstrated using the yeast two-hybrid system. Further analysis restricted the epitope responsible for the binding within the 74 N-terminal amino acids of cyclin D3, independent of the LXCXE amino acid motif present in the D-type cyclin N-terminal region. In a coprecipitation assay in T98G cells, a human glioblastoma cell...<br/><br/>F Bonetto, M Fanciulli, T Battista, A De Luca, P Russo, T Bruno, R De Angelis, M Di Padova, A Giordano, A Felsani, M G Paggi (1999) <i>J Cell Biochem</i> <i></i> <i></i> 75: 4 698-709<br/>

http://publicationslist.org/marco.paggi/refid41 2008-04-10T16:16:42Z

The retinoblastoma family of nuclear factors is composed of RB, the prototype of the tumour suppressor genes and of the strictly related genes p107 and Rb2/p130. The three genes code for proteins, namely pRb, p107 and pRb2/p130, that share similar structures and functions. These proteins are expressed, often simultaneously, in many cell types and are involved in the regulation of proliferation and...<br/><br/>G Raschellà, B Tanno, F Bonetto, A Negroni, P P Claudio, A Baldi, R Amendola, B Calabretta, A Giordano, M G Paggi (1998) <i>Cell Death Differ</i> <i></i> <i></i> 5: 5 401-407<br/>

http://publicationslist.org/marco.paggi/refid42 2008-04-10T16:16:42Z

The effect of the antitumor drug lonidamine (LND) on respiration, aerobic glycolysis, adenylate pool, doxorubicin (DOX) uptake, and efflux in DOX-resistant and DOX-sensitive Ehrlich tumor cells was investigated. The results may be summarized as follows: 1) In both types of cells, LND inhibited both respiration and glycolysis in a dose-dependent manner and lowered the ATP concentration. The effect ...<br/><br/>A Floridi, T Bruno, S Miccadei, M Fanciulli, A Federico, M G Paggi (1998) <i>Biochem Pharmacol</i> <i></i> <i></i> 56: 7 841-849<br/>

http://publicationslist.org/marco.paggi/refid45 2008-04-10T16:16:42Z

B-MYB expression is associated with cell proliferation and recent studies have suggested that it promotes the S phase of mammalian cells. Based on its homology to the transcription factors c-MYB and A-MYB, B-MYB is thought to be involved in transcriptional regulation; however, its activity is not detectable in several cell lines. It was postulated that B-MYB function may depend on the presence of ...<br/><br/>A Sala, M Kundu, I Casella, A Engelhard, B Calabretta, L Grasso, M G Paggi, A Giordano, R J Watson, K Khalili, C Peschle (1997) <i>Proc Natl Acad Sci U S A</i> <i></i> <i></i> 94: 2 532-536<br/>

http://publicationslist.org/marco.paggi/refid44 2008-04-10T16:16:42Z

We studied the effect of phosphorothioate oligodeoxynucleotides ([S]ODNs) complementary to the bcr-abl junction on cells taken at diagnosis from 41 patients with Philadelphia-positive chronic myelogenous leukaemia (CML). Experiments included the evaluation of the anti-leukaemic effect of 16- and 26-mer antisense [S]ODNs on both mononuclear and CD34+ cells, evaluation of incubation time and correla...<br/><br/>P de Fabritiis, T Skorski, M S De Propris, M G Paggi, M Nieborowska-Skorska, A Lisci, S Buffolino, K Campbell, T Geiser, B Calabretta (1997) <i>Leukemia</i> <i></i> <i></i> 11: 6 811-819<br/>

http://publicationslist.org/marco.paggi/refid43 2008-04-10T16:16:42Z

Neuroblastoma cells can undergo neural differentiation upon treatment with a variety of chemical inducers and growth factors. During this process, many cell cycle-related genes are downregulated while differentiation-specific genes are triggered. The retinoblastoma family proteins, pRb, p107, and pRb2/p130, are involved in transcriptional repression of proliferation genes, mainly through their int...<br/><br/>G Raschellà, B Tanno, F Bonetto, R Amendola, T Battista, A De Luca, A Giordano, M G Paggi (1997) <i>J Cell Biochem</i> <i></i> <i></i> 67: 3 297-303<br/>

http://publicationslist.org/marco.paggi/refid49 2008-04-10T16:16:42Z

The effect of the local anesthetic bupivacaine on transmembrane potential of mitochondria within Ehrlich ascites tumor cells has been investigated by the safranine method. The following summarizes the results. 1) When Ehrlich ascites tumor cells were added into a medium containing safranine, a differential spectrum with a decrease at 520 nm appeared. The addition of bupivacaine did not reverse the...<br/><br/>R Pulselli, E Arcuri, M G Paggi, A Floridi (1996) <i>Oncol Res</i> <i></i> <i></i> 8: 7-8 267-271<br/>

http://publicationslist.org/marco.paggi/refid50 2008-04-10T16:16:42Z

Respiration, glycolysis, utilization of carbon from 14C-labeled glucose and the activities of some regulatory enzymes of the Krebs cycle and glycolysis in adriamycin-sensitive (EH-WT) and -resistant (EH-ADR) Ehrlich ascites tumor cells have been investigated. The following summarizes the results: 1. Compared with wild-type cells, EH-ADR cells exhibited an enhanced rate of oxygen consumption as wel...<br/><br/>S Miccadei, M Fanciulli, T Bruno, M G Paggi, A Floridi (1996) <i>Oncol Res</i> <i></i> <i></i> 8: 1 27-35<br/>

http://publicationslist.org/marco.paggi/refid48 2008-04-10T16:16:42Z

The retinoblastoma (Rb) family consists of the tumor suppressor pRb and related proteins p107 and pRb2/p130. Ectopic expression of pRb and p107 results in a growth arrest of sensitive cells in the G1 phase of the cell cycle. We demonstrated here that the growth-suppressive properties of pRb2/p130 were also specific for the G1 phase. The A-, E-, and D-type cyclins as well as transcription factor E2...<br/><br/>P P Claudio, A De Luca, C M Howard, A Baldi, E J Firpo, A Koff, M G Paggi, A Giordano (1996) <i>Cancer Res</i> <i></i> <i></i> 56: 9 2003-2008<br/>

http://publicationslist.org/marco.paggi/refid46 2008-04-10T16:16:42Z

The retinoblastoma gene (RB1) is frequently deleted or mutated in many tumor types and in all cases of retinoblastoma. Apart from its role in regulation of the cell cycle, the RB1 gene product (p110RB1) appears to be involved in control of differentiation. Malignant metastatic cells show many properties of poorly differentiated cells, and are highly invasive in vitro and in vivo. We have transfect...<br/><br/>P Valente, A Melchiori, M G Paggi, L Masiello, D Ribatti, L Santi, R Takahashi, A Albini, D M Noonan (1996) <i>Oncogene</i> <i></i> <i></i> 13: 6 1169-1178<br/>

http://publicationslist.org/marco.paggi/refid47 2008-04-10T16:16:42Z

Two genes, p107 and Rb2/p130, are strictly related to RB, the most investigated tumor suppressor gene, responsible for susceptibility to retinoblastoma. The products of these three genes, namely pRb, p107, and pRb2/p130 are characterized by a peculiar steric conformation, called "pocket," responsible for most of the functional interactions characterizing the activity of these proteins in the homeo...<br/><br/>M G Paggi, A Baldi, F Bonetto, A Giordano (1996) <i>J Cell Biochem</i> <i></i> <i></i> 62: 3 418-430<br/>

http://publicationslist.org/marco.paggi/refid53 2008-04-10T16:16:42Z

We have described previously three mouse multifinger coding genes (C. Passananti et al., Proc. Natl. Acad. Sci. USA, 86: 9417-9421, 1989). We have analyzed the expression of one of them, termed Mfg2 and renamed Zfp59, and demonstrated that Zfp59 mRNA and its translation product are present in specific stages of mouse spermatogenesis. The predicted amino acid sequence of the Zfp59 protein has been ...<br/><br/>C Passananti, N Corbi, M G Paggi, M A Russo, M Perez, F Cotelli, M Stefanini, P Amati (1995) <i>Cell Growth Differ</i> <i></i> <i></i> 6: 8 1037-1044<br/>

http://publicationslist.org/marco.paggi/refid52 2008-04-10T16:16:42Z

The retinoblastoma susceptibility gene in leukemia and lymphoma has been investigated using different approaches involving either gene or protein analysis. In this study, a novel method, which evaluates the functional status of the retinoblastoma gene product by a binding assay to an in vitro-translated viral oncoprotein, has been applied to leukemic cells from acute myeloid leukemia patients. One...<br/><br/>M G Paggi, P de Fabritiis, F Bonetto, L Amadio, G Santarelli, A Spadea, F P Gentile, A Floridi, A Felsani (1995) <i>Cancer Res</i> <i></i> <i></i> 55: 20 4552-4556<br/>

http://publicationslist.org/marco.paggi/refid51 2008-04-10T16:16:42Z

The Rb2/p130 protein has been shown to have a high sequence homology with the retinoblastoma gene product (pRb), one of the most well-characterized tumor suppressor genes, and with pRB-related p107, especially in their conserved pocket domains, which display a primary role in the function of these proteins. In this study, we report on the biochemical and immunocytochemical characterization of the ...<br/><br/>A Baldi, A De Luca, P P Claudio, F Baldi, G G Giordano, M Tommasino, M G Paggi, A Giordano (1995) <i>J Cell Biochem</i> <i></i> <i></i> 59: 3 402-408<br/>

http://publicationslist.org/marco.paggi/refid55 2008-04-10T16:16:42Z

The glycolytic enzyme hexokinase plays a key role in regulating cell energy metabolism. Its activity has been associated with cell growth rate and, notably, with neoplastic transformation. NIH-3T3 cells were transfected with a tumor hexokinase cDNA. The transfected cells showed increased hexokinase amount and activity, mainly located in the particulate cellular fraction, increased glycolytic rate ...<br/><br/>M Fanciulli, M G Paggi, T Bruno, C Del Carlo, F Bonetto, F P Gentile, A Floridi (1994) <i>Oncol Res</i> <i></i> <i></i> 6: 9 405-409<br/>

http://publicationslist.org/marco.paggi/refid54 2008-04-10T16:16:42Z

Inactivating mutations of the retinoblastoma susceptibility gene (Rb) are involved in the pathogenesis of hereditary and sporadic retinoblastoma. Alterations in the Rb gene have also been found in several other human tumors occurring with epidemiological incidence higher than that of retinoblastoma. Four human malignant glioma cell lines were examined for abnormalities in the retinoblastoma gene p...<br/><br/>M G Paggi, F Martelli, M Fanciulli, A Felsani, S Sciacchitano, M Varmi, T Bruno, C M Carapella, A Floridi (1994) <i>Cancer Res</i> <i></i> <i></i> 54: 4 1098-1104<br/>

http://publicationslist.org/marco.paggi/refid58 2008-04-10T16:16:42Z

The utilization of carbon from 14C-labeled glucose by adriamycin (ADM)-sensitive and -resistant LoVo human colon carcinoma cells has been investigated. The following summarizes the results: 1. Aerobic glycolysis is the main energy-yielding process in both cell lines, whereas only a small proportion of glucose carbon atoms are incorporated into CO2, lipids, nucleic acids, and supporting structures....<br/><br/>M Fanciulli, T Bruno, S Castiglione, C Del Carlo, M G Paggi, A Floridi (1993) <i>Oncol Res</i> <i></i> <i></i> 5: 9 357-362<br/>

http://publicationslist.org/marco.paggi/refid57 2008-04-10T16:16:42Z

The effect of hyperthermia and lonidamine, alone and in combination, on the clonogenic activity of a human glioma cell line was investigated. The time-temperature relationship of asynchronous, exponentially growing cells was defined in the range of 40-45 degrees C. All survival curves were exponential and an Arrhenius plot for heat killing was linear over the temperature range tested, with an acti...<br/><br/>A Floridi, F P Gentile, T Bruno, A Delpino, C Iacobini, M G Paggi, S Castiglione, M Benassi (1993) <i>Oncol Res</i> <i></i> <i></i> 5: 1 1-10<br/>

http://publicationslist.org/marco.paggi/refid56 2008-04-10T16:16:42Z

Rhein (RH), 4,5 dihydroxyanthraquinone-2-carboxylic acid, is known to inhibit the glycolysis of neoplastic cells by impairing glucose uptake. In order to establish whether this might be due to a selective interaction of the carrier with the drug or to functional modifications of the cell membrane, the effect of RH on glucose uptake in Ehrlich ascites tumor cells has been investigated. RH strongly ...<br/><br/>S Castiglione, M Fanciulli, T Bruno, M Evangelista, C Del Carlo, M G Paggi, A Chersi, A Floridi (1993) <i>Anticancer Drugs</i> <i></i> <i></i> 4: 3 407-414<br/>

http://publicationslist.org/marco.paggi/refid60 2008-04-10T16:16:42Z

The effect of Rhein (RH) on the protein synthetic activity and adenylate energy charge in human glioma cells cultured in vitro has been investigated. The results demonstrate that in RH-treated cells, the protein synthesis is strongly decreased, but no modifications in the qualitative pattern occur. The extent of inhibition is a function of the drug concentration as well as of the time of exposure....<br/><br/>A Delpino, M G Paggi, P F Gentile, S Castiglione, T Bruno, M Benass, A Floridi (1992) <i>Cancer Biochem Biophys</i> <i></i> <i></i> 12: 4 241-252<br/>

http://publicationslist.org/marco.paggi/refid59 2008-04-10T16:16:42Z

The effect of the combination of adriamycin (ADM) with the anti-inflammatory drug rhein (RH) on the membrane redox activity in human glioma cells was investigated. RH, although less effective than ADM, inhibits ferricyanide reduction by human glioma cells in a dose-dependent manner as well as ferricyanide-induced proton release. The inhibition of the plasma membrane redox system might represent a ...<br/><br/>M Fanciulli, F P Gentile, T Bruno, M G Paggi, M Benassi, A Floridi (1992) <i>Anticancer Drugs</i> <i></i> <i></i> 3: 6 615-621<br/>

http://publicationslist.org/marco.paggi/refid61 2008-04-10T16:16:42Z

In Northern blot analysis of a series of tumor cell lines a single hexokinase mRNA species of 4.3 Kb was detected. Detailed examination of one such line, the rat AS-30D hepatoma, revealed that two mitochondrial species of hexokinase are present with a molecular mass of 115 and 107 KDa. The smaller of the two species is 4-fold more active than the larger. Only the larger, less active species is det...<br/><br/>M G Paggi, M Fanciulli, C Del Carlo, G Citro, T Bruno, A Floridi (1991) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 178: 2 648-655<br/>

http://publicationslist.org/marco.paggi/refid62 2008-04-10T16:16:42Z

The effect of association of 1,3-bis-(2-chloroethyl)-l-nitrosourea (BCNU)- Rhein (RH) and BCNU-Lonidamine (LND) on the clonogenic activity of human glioma cells was evaluated. Both RH and LND modulate the lethal effect of BCNU regardless of the schedule of treatment. The analysis of the interactions, performed with the isbolar method according to Berembaum, demonstrates an additivity of the effect...<br/><br/>A Floridi, P F Gentile, T Bruno, M Fanciulli, M G Paggi, M Zeuli, M Benassi (1991) <i>Anticancer Res</i> <i></i> <i></i> 11: 2 789-792<br/>

http://publicationslist.org/marco.paggi/refid65 2008-04-10T16:16:42Z

Hexokinase is a key enzyme in carbohydrate metabolism. Its activity has been shown elevated in cells with high mitotic index. In particular, experimental cancer cells, due to their peculiar energy metabolism, display a hexokinase activity proportional to the degree of malignancy. This is the case also for human gliomas in which glucose metabolism, evaluated via positron emission tomography, has be...<br/><br/>M G Paggi, M Fanciulli, C Del Carlo, G Citro, C M Carapella, A Floridi (1990) <i>J Neurosurg Sci</i> <i></i> <i></i> 34: 3-4 209-213<br/>

http://publicationslist.org/marco.paggi/refid64 2008-04-10T16:16:42Z

Lonidamine (LND) is a drug that interferes with energy metabolism of cancer cells, principally inhibiting aerobic glycolytic activity, by its effect on mitochondrially-bound hexokinase (HK). In such way LND could impair energy-requiring processes, as recovery from potentially lethal damage, induced by radiation treatment and by some cytotoxic drugs. A randomized study started in November 1983, to ...<br/><br/>C M Carapella, M G Paggi, F Calvosa, F Cattani, B Jandolo, R Mastrostefano, L Raus, A Riccio (1990) <i>J Neurosurg Sci</i> <i></i> <i></i> 34: 3-4 261-264<br/>

http://publicationslist.org/marco.paggi/refid66 2008-04-10T16:16:42Z

Recent data from phase II trials have shown that lonidamine (LND) is effective in the treatment of tumors of various histogenesis, including gliomas. In the present work, we tested the antiproliferative effect of LND on a human glioblastoma cell line (LI) in different culture conditions. When LI are cultured in their standard conditions, a reduction of cell growth is seen after 3 days of treatment...<br/><br/>F Iacopino, G Sica, P Macrì, M G Paggi, M Scerrati, R Roselli, P Marchetti, G R Della Cuna, L Marini (1990) <i>J Neurosurg Sci</i> <i></i> <i></i> 34: 3-4 193-197<br/>

http://publicationslist.org/marco.paggi/refid63 2008-04-10T16:16:42Z

The action of rhein, 4,5-dihydroxyanthraquinone-2-carboxylic acid, on protein synthesis of neoplastic cells has been investigated. Rhein decreases amino acid incorporation in all cells tested. The inhibition of incorporation of labeled precursors into acid-insoluble material cannot be ascribed to an impairment of amino acid uptake, which is unaffected by the drug. Tests on cell-free system showed ...<br/><br/>S Castiglione, M G Paggi, A Delpino, M Zeuli, A Floridi (1990) <i>Biochem Pharmacol</i> <i></i> <i></i> 40: 5 967-973<br/>

http://publicationslist.org/marco.paggi/refid69 2008-04-10T16:16:42Z

Among the features of the reprogrammed neoplastic phenotype there is the metabolic property to display an increased glycolytic capacity and the ability to convert glucose to lactic acid in the presence of oxygen. Human gliomas in vivo and in vitro are capable to metabolize glucose in a way strictly related to the pathological degree of malignancy. The drug Lonidamine [1-(2,4-dichlorobenzyl)-1H-ind...<br/><br/>A Floridi, M G Paggi, M Fanciulli (1989) <i>J Neurosurg Sci</i> <i></i> <i></i> 33: 1 55-64<br/>

http://publicationslist.org/marco.paggi/refid68 2008-04-10T16:16:42Z

Receptors for Insulin, Epidermal Growth Factor, Platelet-Derived Growth Factor and Insulin-like Growth Factor type 1 are tyrosine-specific protein kinases. This enzymatic activity may play a role in mediating the biological actions of these peptides. It has recently been identified a Mr 120 KDa glycoprotein in rat liver plasma membranes which can be phosphorylated by the insulin receptor and by th...<br/><br/>M Fanciulli, M G Paggi, A Mancini, C Del Carlo, A Floridi, S I Taylor, N Perrotti (1989) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 160: 1 168-173<br/>

http://publicationslist.org/marco.paggi/refid67 2008-04-10T16:16:42Z

Up-to-date unsatisfactory results obtained in multimodality treatments of malignant glioma have prompted the research of new therapeutic modalities with 'unconventional' modes of action. Lonidamine (LND) is a drug which reduces aerobic glycolytic activity in both human and experimental tumors. This effect mainly depends on the inhibition of mitochondrially-bound hexokinase (HK) which is present in...<br/><br/>C M Carapella, M G Paggi, F Cattani, G B Ciottoli, A Floridi, B Iandolo, L Raus, A Riccio, A Caputo (1989) <i>J Neurooncol</i> <i></i> <i></i> 7: 1 103-108<br/>

http://publicationslist.org/marco.paggi/refid73 2008-04-10T16:16:42Z

M G Paggi, M Fanciulli, N Perrotti, A Floridi, M Zeuli, B Silvestrini, A Caputo (1988) <i>Ann N Y Acad Sci</i> <i></i> <i></i> 551: 358-360<br/>

http://publicationslist.org/marco.paggi/refid71 2008-04-10T16:16:42Z

Lonidamine (LND) has been shown to inhibit tumor aerobic glycolysis. Its effect was evaluated on several human astrocytomas at different degrees of malignancy; a correlation was found between LDN effect on lactate production and tumor malignancy: in grade I and II astrocytomas LND stimulates lactate production, while in grade III, IV and glioblastoma multiforme lactate production is inhibited. In ...<br/><br/>M G Paggi, C M Carapella, M Fanciulli, C Del Carlo, S Giorno, G Zupi, B Silvestrini, A Caputo, A Floridi (1988) <i>J Neurooncol</i> <i></i> <i></i> 6: 3 203-209<br/>

http://publicationslist.org/marco.paggi/refid72 2008-04-10T16:16:42Z

Recent studies from this laboratory have demonstrated that a form of hexokinase characteristic of rapidly growing, highly glycolytic tumor cells is bound to an outer mitochondrial membrane receptor complex containing a Mr 35,000 pore protein (D. M. Parry and P. L. Pedersen, J. Biol. Chem., 258: 10904-10912, 1983; R. A. Nakashima, et al., Biochemistry, 25: 1015-1021, 1986). In new studies reported ...<br/><br/>R A Nakashima, M G Paggi, L J Scott, P L Pedersen (1988) <i>Cancer Res</i> <i></i> <i></i> 48: 4 913-919<br/>

http://publicationslist.org/marco.paggi/refid70 2008-04-10T16:16:42Z

The effect of Lonidamine, 1-(2,4 dichlorobenzyl)-1-H-indazol-3-carboxylic acid, on the uptake of Adriamycin by Ehrlich ascites tumor cells has been investigated. The uptake of Adriamycin is greatly stimulated by Lonidamine and the increase depends on the energy sources of the cell. In the presence of glucose the intracellular drug content is remarkably lower than that in its absence. This differen...<br/><br/>A Floridi, A Gambacurta, A Bagnato, C Bianchi, M G Paggi, B Silvestrini, A Caputo (1988) <i>Exp Mol Pathol</i> <i></i> <i></i> 49: 3 421-431<br/>

http://publicationslist.org/marco.paggi/refid76 2008-04-10T16:16:42Z

The effect of hyperthermia (1 hr, 41 degrees C) on the functional properties of Ehrlich ascites tumor mitochondria was investigated. Mitochondria isolated from Ehrlich ascites tumor after exposure of whole cells to 41 degrees C for 1 hr still phosphorylate and maintain a normal acceptor control ratio (ACR). The temperature decreases state 4 and ADP-and FCCP-stimulated respiration on various substr...<br/><br/>A Floridi, A Nista, M G Paggi, P L Pellegrini, A Bagnato, M Fanciulli, A Caputo (1987) <i>Exp Mol Pathol</i> <i></i> <i></i> 46: 3 279-293<br/>

http://publicationslist.org/marco.paggi/refid75 2008-04-10T16:16:42Z

The effect of lonidamine (LND), 1-(2,4-dichlorobenzyl)-1H-indazol-3 carboxylic acid, on the utilization of carbon from 14C-labeled glucose by cell cultures of the permanent strain LI derived from a human glioblastoma multiforme (astrocytoma) has been investigated. The results may be summarized as follows. Aerobic glycolysis is the main energy-yielding process as shown by the fact that the greatest...<br/><br/>M G Paggi, G Zupi, M Fanciulli, C Del Carlo, S Giorno, N Laudonio, B Silvestrini, A Caputo, A Floridi (1987) <i>Exp Mol Pathol</i> <i></i> <i></i> 47: 2 154-165<br/>

http://publicationslist.org/marco.paggi/refid77 2008-04-10T16:16:42Z

The effect of Adriamycin on mitochondria of the rat heart, liver, and Ehrlich ascites tumor mitochondria has been evaluated. The results may be summarized as follows: Adriamycin reduces both ADP- and FCCP-stimulated respiration, inhibits oxidative phosphorylation, decreases mitochondrial ATP-ase activity, and affects the redox state of respiratory carriers. These alterations are common to all type...<br/><br/>C Bianchi, A Bagnato, M G Paggi, A Floridi (1987) <i>Exp Mol Pathol</i> <i></i> <i></i> 46: 1 123-135<br/>

http://publicationslist.org/marco.paggi/refid74 2008-04-10T16:16:42Z

Low concentrations of Adriamycin (ADM) do not inhibit the oxygen consumption of rat liver mitochondria because of the inability to cross the outer membrane. The involvement of this membrane as a permeability barrier is demonstrated by the results with exogenous ferrocytochrome c. ADM does not affect the basal rate of ferrocytochrome c oxidation, which, on the contrary, increases in Lonidamine (LND...<br/><br/>A Floridi, C Bianchi, A Bagnato, A Gambacurta, M G Paggi, B Silvestrini, A Caputo (1987) <i>Anticancer Res</i> <i></i> <i></i> 7: 6 1149-1152<br/>

http://publicationslist.org/marco.paggi/refid80 2008-04-10T16:16:42Z

Lonidamine induces in murine and human tumor cells severe morphological damage of the mitochondria and other cytoplasmic structures both 'in vitro' and 'in vivo'. Biochemical studies have demonstrated that the drug decreases oxygen consumption and lactate production. The sensitivity of human tumor cells is not related to their histotype. Lonidamine's effects on mitochondria, glycolysis, pentose ph...<br/><br/>C De Martino, T Battelli, M G Paggi, A Nista, M L Marcante, S D'Atri, W Malorni, M Gallo, A Floridi (1984) <i>Oncology</i> <i></i> <i></i> 41 Suppl 1: 15-29<br/>

http://publicationslist.org/marco.paggi/refid79 2008-04-10T16:16:42Z

The effect of the association of gossypol and Lonidamine on the electron transport in Ehrlich ascites tumor mitochondria has been investigated by addition of drugs to isolated mitochondria. The results may be summarized as follows. (1) Low concentrations of gossypol increase the rate of oxygen consumption at the level of three energy-conserving sites of the respiratory chain. Higher concentrations...<br/><br/>A Floridi, S D'Atri, M Bellocci, M L Marcante, M G Paggi, B Silvestrini, A Caputo, C De Martino (1984) <i>Exp Mol Pathol</i> <i></i> <i></i> 40: 2 246-261<br/>

http://publicationslist.org/marco.paggi/refid78 2008-04-10T16:16:42Z

The AS-30D rat hepatoma cell line is characteristic of that class of rapidly growing tumors which exhibit high rates of aerobic glucose utilization and lactic acid production (Bustamante, E., Morris, H.P., and Pedersen, P.L., J. Biol. Chem., 256: 8699-8704, 1981). In this study, we have examined the coupling properties of the mitochondria in intact AS-30D hepatoma cells and the relative contributi...<br/><br/>R A Nakashima, M G Paggi, P L Pedersen (1984) <i>Cancer Res</i> <i></i> <i></i> 44: 12 Pt 1 5702-5706<br/>

http://publicationslist.org/marco.paggi/refid82 2008-04-10T16:16:42Z

The action of Lonidamine [1-(2,4-chlorobenzyl)-1H-indazol-3-carboxylic acid] on respiration and aerobic lactate production of several murine tumor cells and normal differentiated murine cells was investigated. Lonidamine reduced the oxygen consumption in both normal and neoplastic cells. In contrast, it increased the aerobic glycolysis of normal cells but inhibited that of tumor cells. This select...<br/><br/>A Floridi, M G Paggi, M L Marcante, B Silvestrini, A Caputo, C De Martino (1981) <i>J Natl Cancer Inst</i> <i></i> <i></i> 66: 3 497-499<br/>

http://publicationslist.org/marco.paggi/refid83 2008-04-10T16:16:42Z

A Floridi, M Bellocci, M G Paggi, M L Marcante, C De Martino (1981) <i>Chemotherapy</i> <i></i> <i></i> 27 Suppl 2: 50-60<br/>

http://publicationslist.org/marco.paggi/refid81 2008-04-10T16:16:42Z

The action of Lonidamine [1-(2,4-dichlorobenzyl)-1-H-indazol-3-carboxylic acid] on oxygen consumption and the rate of aerobic and anaerobic lactate production by Ehrlich ascites tumor cells has been investigated. The rate of oxygen consumption decreases exponentially with the increase of Lonidamine concentration, with maximal inhibition occurring at 0.40 mM Lonidamine. The rate of aerobic lactate ...<br/><br/>A Floridi, M G Paggi, S D'Atri, C De Martino, M L Marcante, B Silvestrini, A Caputo (1981) <i>Cancer Res</i> <i></i> <i></i> 41: 11 Pt 1 4661-4666<br/>