maria elena gelain's Publications List

What is your diagnosis? Blood smear from a dog.

2011-11-25T19:29:54Z

Melania Bardoneschi, Vittorio Garbagnoli, Luigi Venco, Maria Elena Gelain, Walter Bertazzolo (2011) Vet Clin Pathol 40: 2 271-272

Splenic marginal zone lymphoma in 5 dogs (2001-2008).

2011-04-08T19:05:08Z

Splenic marginal zone lymphomas (MZL) in dogs arise from the marginal zone of B-cell follicles and can progress slowly.

D Stefanello, P Valenti, E Zini, S Comazzi, M E Gelain, P Roccabianca, G Avallone, M Caniatti, L Marconato (2011) J Vet Intern Med 25: 1 90-93

Immunophenotype predicts survival time in dogs with chronic lymphocytic leukemia.

2011-04-08T19:05:08Z

Chronic lymphocytic leukemia (CLL) is a hematologic disorder in dogs, but studies on prognostic factors and clinical outcome are lacking. In people, several prognostic factors have been identified and currently are used to manage patients and determine therapy.

S Comazzi, M E Gelain, V Martini, F Riondato, B Miniscalco, L Marconato, D Stefanello, M Mortarino (2011) J Vet Intern Med 25: 1 100-106

Immunophenotype-related microRNA expression in canine chronic lymphocytic leukemia.

2011-11-25T19:29:54Z

MicroRNAs (miRNAs) are posttranscriptional regulatory noncoding RNAs used to profile human hematopoietic tumors. In this study, some mature miRNAs was quantitated in peripheral blood from dogs with chronic lymphocytic leukemia (CLL). Relative expression data were normalised against four endogenous controls (let-7a, miR-17-5p, miR-26b, and miR-223) selected by geNorm analysis. The results revealed ...

G Gioia, M Mortarino, M E Gelain, F Albonico, E Ciusani, I Forno, L Marconato, V Martini, S Comazzi (2011) Vet Immunol Immunopathol 142: 3-4 228-235

Identification of suitable endogenous controls and differentially expressed microRNAs in canine fresh-frozen and FFPE lymphoma samples.

2011-04-08T19:05:08Z

The elucidation of microRNA (miRNA) expression pattern in canine lymphoma is attractive for veterinary and comparative oncology due to similar genetics, physiology and exposure to environment in dogs and humans. In this work, the expression of a panel of mature miRNAs was quantitated in fresh-frozen and formalin-fixed paraffin-embedded (FFPE) lymph nodes from canine lymphoma. The major findings we...

M Mortarino, G Gioia, M E Gelain, F Albonico, P Roccabianca, E Ferri, S Comazzi (2010) Leuk Res 34: 8 1070-1077

Identification of neoplastic cells in blood using the Sysmex XT-2000iV: a preliminary step in the diagnosis of canine leukemia.

2011-04-08T19:05:08Z

Classification of leukemias requires specialized diagnostic techniques. Automated preliminary indicators of neoplastic cells in blood would expedite selection of appropriate tests.

Maria Elena Gelain, Gabriele Rossi, Luca Giori, Stefano Comazzi, Saverio Paltrinieri (2010) Vet Clin Pathol 39: 2 169-179

Acute megakaryoblastic leukemia in dogs: a report of three cases and review of the literature.

2011-04-08T19:05:08Z

Three dogs of different breeds, ages, and genders were presented with pale mucous membranes, depression, anorexia, and splenomegaly. Observed were severe normocytic, nor-mochromic, nonregenerative anemia, thrombocytopenia, and leukopenia. Blood smears contained large, atypical cells with blue vacuolated cytoplasm, cytoplasmic blebs, round to oval central nuclei, and elevated numbers of cytoplasmic...

Stefano Comazzi, Maria E Gelain, Ugo Bonfanti, Paola Roccabianca (2010) J Am Anim Hosp Assoc 46: 5 327-335

Use of flow cytometric immunophenotyping to refine the cytological diagnosis of canine lymphoma.

2011-04-08T19:05:08Z

The diagnosis of canine lymphoma is a multi-step process involving clinical examination, diagnostic imaging, cytology, haematology, biochemical profiling, histopathology and ancillary techniques such as flow cytometry (FC). In human medicine, FC (in addition to cytology) is reported to increase the accuracy of diagnosis of most lymphoma sub-types. In dogs, FC can add a number of useful diagnostic ...

S Comazzi, M E Gelain (2010) Vet J :

Macrothrombocytopenia in a group of related Norfolk terriers.

2011-04-08T19:05:08Z

M E Gelain, G F Tutino, E Pogliani, W Bertazzolo (2010) Vet Rec 167: 13 493-494

Disseminated histiocytic sarcoma with peripheral blood involvement in a Bernese Mountain dog.

2011-04-08T19:05:08Z

A 6-year-old Bernese Mountain dog was presented with a history of lethargy and weight loss of 2 weeks duration. On physical examination the dog had pale mucous membranes and tachypnea. Ultrasound examination revealed hepatomegaly, splenomegaly, and mesenteric lymphadenomegaly. Results of a CBC included marked normocytic normochromic nonregenerative anemia, marked thrombocytopenia, and moderate leu...

Silvia Rossi, Maria Elena Gelain, Stefano Comazzi (2009) Vet Clin Pathol 38: 1 126-130

ZAP-70 and Syk expression in canine lymphoid cells and preliminary results on leukaemia cases.

2011-04-08T19:05:08Z

Zeta-chain-associated protein (ZAP-70) and spleen tyrosine kinase (Syk) are structurally and functionally homologous tyrosine kinases playing a role in the T- and B-cell signal transduction. Their activation can lead to lymphokine production, cytolitic activity, antibody secretion, cell proliferation, differentiation, survival and phagocytosis. Anomalous ZAP-70 and Syk expression is reported to be...

M Mortarino, M E Gelain, G Gioia, E Ciusani, C Bazzocchi, S Comazzi (2009) Vet Immunol Immunopathol 128: 4 395-401

Extreme monocytosis in a dog with chronic monocytic leukaemia.

2011-04-08T19:05:08Z

G Rossi, M E Gelain, S Foroni, S Comazzi (2009) Vet Rec 165: 2 54-56

Association between faecal shedding of feline coronavirus and serum alpha1-acid glycoprotein sialylation.

2011-04-08T19:06:01Z

The sialylation pattern of serum alpha1-acid glycoprotein (AGP) in non-symptomatic cats infected by feline coronavirus (FCoV) and its possible relationship with the amount of FCoVs shed in faeces were investigated. Blood from three specific pathogen-free cats (group A) and from 10 non-symptomatic FCoV-positive cats from catteries with low (group B, three cats) or high (group C, seven cats) levels ...

Saverio Paltrinieri, Maria E Gelain, Fabrizio Ceciliani, Alba M Ribera, Mara Battilani (2008) J Feline Med Surg 10: 5 514-518

Aberrant phenotypes and quantitative antigen expression in different subtypes of canine lymphoma by flow cytometry.

2011-04-08T19:06:01Z

Flow cytometry may be a useful tool to analyze lymphoma samples that are obtained from fine needle aspirations (FNA). This study aimed to determine if flow cytometric analysis add more objective and standardized information on the cellularity and morphology of lymphoma cells to conventional cytology. The typical immunophenotype of different lymphoma subtypes was assessed and leukocyte marker expre...

M E Gelain, M Mazzilli, F Riondato, L Marconato, S Comazzi (2008) Vet Immunol Immunopathol 121: 3-4 179-188

Serum alpha1-acid glycoprotein (AGP) concentration in non-symptomatic cats with feline coronavirus (FCoV) infection.

2011-04-08T19:06:01Z

Previous studies have demonstrated that the concentration of alpha1-acid glycoprotein (AGP) transiently increases in asymptomatic cats infected with feline coronavirus (FCoV). In order to establish whether these fluctuations depend on the FCoV status, the serum concentration of AGP and anti-FCoV antibody titres and/or faecal shedding of FCoVs in clinically healthy cats from catteries with differen...

Saverio Paltrinieri, Cecilia Metzger, Mara Battilani, Vanessa Pocacqua, Maria Elena Gelain, Alessia Giordano (2007) J Feline Med Surg 9: 4 271-277

Flow cytometric expression of common antigens CD18/CD45 in blood from dogs with lymphoid malignancies: a semi-quantitative study.

2011-04-08T19:06:01Z

Flow cytometry is useful to study lymphoid malignancies since it allows both immunophenotyping of neoplastic cells and quantification of antigen expression. CD18 and CD45 are commonly exposed membrane antigens with different levels of expression on blood leukocyte and neoplastic cells. The aim of this retrospective study was to semi-quantitatively evaluate the expression of CD18 and CD45 in dogs w...

S Comazzi, M E Gelain, F Riondato, S Paltrinieri (2006) Vet Immunol Immunopathol 112: 3-4 243-252

Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45.

2011-04-08T19:06:01Z

In dogs, flow cytometry is used in the phenotyping of immunologic cells and in the diagnosis of hemic neoplasia. However, the paucity of specific antibodies for myeloid cells and B lymphocytes and of labeled antibodies for multicolor techniques limits the ability to detect all leukocyte subpopulations. This is especially true for neoplastic and precursor cells. CD18 and CD45 are expressed on all l...

Stefano Comazzi, M Elena Gelain, Valentina Spagnolo, Fulvio Riondato, Roberta Guglielmino, Paola Sartorelli (2006) Vet Clin Pathol 35: 1 47-54

Whole blood cytokine profiles in cats infected by feline coronavirus and healthy non-FCoV infected specific pathogen-free cats.

2011-04-08T19:06:01Z

In this study, the cytokine profiles of clinically healthy cats naturally infected with feline coronavirus (FCoV), of cats with feline infectious peritonitis (FIP) and of specific pathogen-free (SPF) cats were investigated in whole blood using a traditional reverse-transcriptase polymerase chain reaction (RT-PCR) assay and a semi-quantitative method of analysis based on computerised quantification...

Maria E Gelain, Marina Meli, Saverio Paltrinieri (2006) J Feline Med Surg 8: 6 389-399

Chronic eosinophilic leukemia in a cat: cytochemical and immunophenotypical features.

2011-04-08T19:06:01Z

A 3-year-old, male, domestic shorthaired cat was presented with a 3-day history of anorexia and depression. The cat was moderately dehydrated, had pale, slightly icteric, mucous membranes, oral ulcerations, and mild hepatosplenomegaly. A feline leukemia virus (FeLV) antigen test was positive. CBC results obtained at initial presentation included severe normocytic, normochromic, nonregenerative ane...

Maria Elena Gelain, Elisa Antoniazzi, Walter Bertazzolo, Maurizia Zaccolo, Stefano Comazzi (2006) Vet Clin Pathol 35: 4 454-459

Glycan moiety modifications of feline alpha1-acid glycoprotein in retrovirus (FIV, FeLV) affected cats.

2011-04-08T19:06:51Z

alpha1-Acid glycoprotein (AGP) is considered one of the major acute phase proteins in cats. In humans, AGP is a heavily glycosylated protein that undergoes several modifications of its glycan moiety during acute and chronic inflammatory pathologies. In this paper we present the feline AGPs (fAGP) glycan moiety modifications in the course of two prevalent feline diseases, the FIV (feline immunodefi...

Vanessa Pocacqua, Elena Provasi, Saverio Paltrinieri, Elena Gelain, Claudio Comunian, Fabrizio Ceciliani (2005) Vet Immunol Immunopathol 107: 1-2 17-26