http://publicationslist.org/data/massimo.pinto/atom.xml Massimo Pintohttp://publicationslist.org/massimo.pinto$basepathfavicon.icoRecent additions to Massimo Pinto's PublicationsList.org pagehttp://publicationslist.org/publications.png2008-02-05T17:05:14Z http://publicationslist.org/massimo.pinto/refid7 2008-02-05T17:04:20Z

Targeted therapies result in heterogeneous drug delivery, often with highly variable drug uptake in the targeted cells and significant numbers of cells that are essentially untargeted. However both the variably targeted cells and neighboring bystander cells may respond to the treatment. Using ionizing radiation as an example of a targeted therapeutic agent, we describe a quantitative immunofluores...<br/><br/>Massimo Pinto, Roger W Howell (2007) <i>Biotechniques</i> <i></i> <i></i> 43: 1 64, 66-64, 71<br/>

http://publicationslist.org/massimo.pinto/refid8 2008-02-05T17:04:20Z

Prediction of risks and therapeutic outcome in nuclear medicine largely rely on calculation of the absorbed dose. Absorbed dose specification is complex due to the wide variety of radiations emitted, non-uniform activity distribution, biokinetics, etc. Conventional organ absorbed dose estimates assumed that radioactivity is distributed uniformly throughout the organ. However, there have been drama...<br/><br/>R W Howell, P V S V Neti, M Pinto, B I Gerashchenko, V R Narra, E I Azzam (2006) <i>Radiat Prot Dosimetry</i> <i></i> <i></i> 122: 1-4 521-527<br/>

http://publicationslist.org/massimo.pinto/refid9 2008-02-05T17:04:20Z

Research on the radiation-induced bystander effect has been carried out mainly in 2-D tissue culture systems. This study uses a 3-D model, wherein apparently normal human diploid fibroblasts (AG1522) are grown in a carbon scaffold, to investigate the induction of a G(1) checkpoint in bystander cells present alongside radiolabelled cells. Cultures were simultaneously pulse-labelled with (3)H-deoxyc...<br/><br/>M Pinto, E I Azzam, R W Howell (2006) <i>Radiat Prot Dosimetry</i> <i></i> <i></i> 122: 1-4 252-255<br/>

http://publicationslist.org/massimo.pinto/refid1 2008-02-05T17:03:51Z

The rejoining kinetics of double-stranded DNA fragments, along with measurements of residual damage after postirradiation incubation, are often used as indicators of the biological relevance of the damage induced by ionizing radiation of different qualities. Although it is widely accepted that high-LET radiation-induced double-strand breaks (DSBs) tend to rejoin with kinetics slower than low-LET r...<br/><br/>M Pinto, K M Prise, B D Michael (2005) <i>Radiat Res</i> <i></i> <i></i> 164: 1 73-85<br/>

http://publicationslist.org/massimo.pinto/refid2 2008-02-05T17:03:51Z

In studies of radiation-induced DNA fragmentation and repair, analytical models may provide rapid and easy-to-use methods to test simple hypotheses regarding the breakage and rejoining mechanisms involved. The random breakage model, according to which lesions are distributed uniformly and independently of each other along the DNA, has been the model most used to describe spatial distribution of ra...<br/><br/>M Pinto, K M Prise, B D Michael (2004) <i>Radiat Res</i> <i></i> <i></i> 162: 4 453-463<br/>

http://publicationslist.org/massimo.pinto/refid3 2008-02-05T17:03:51Z

When a charged-particle track intercepts the chromatin fibre in DNA of mammalian cells, clustered damage is induced depending on the DNA conformation, local environment and track structure. Intra-track correlated DNA damage may have a higher probability of being mis-repaired or left un-repaired. Fragment size-distributions of DNA double strand breaks (DSBs) induced in primary human fibroblasts by ...<br/><br/>M Pinto, K M Prise, B D Michael (2002) <i>Radiat Prot Dosimetry</i> <i></i> <i></i> 99: 1-4 133-136<br/>

http://publicationslist.org/massimo.pinto/refid4 2008-02-05T17:03:51Z

PURPOSE: To assess the applicability of methods of quantification of double-strand breaks (DSB) based on the random breakage paradigm, measuring yield and distribution of DSB induced by varying radiation quality. MATERIAL AND METHODS: 240 kVp X-rays and (238)Pu alpha-particles were used to induce DSB in AG01522B primary human fibroblasts. DNA molecular weight distributions were resolved by means o...<br/><br/>M Pinto, K M Prise, B D Michael (2002) <i>Int J Radiat Biol</i> <i></i> <i></i> 78: 5 375-388<br/>

http://publicationslist.org/massimo.pinto/refid5 2008-02-05T17:03:51Z

Underpinning current models of the mechanisms of the action of radiation is a central role for DNA damage and in particular double-strand breaks (DSBs). For radiations of different LET, there is a need to know the exact yields and distributions of DSBs in human cells. Most measurements of DSB yields within cells now rely on pulsed-field gel electrophoresis as the technique of choice. Previous meas...<br/><br/>K M Prise, M Pinto, H C Newman, B D Michael (2001) <i>Radiat Res</i> <i></i> <i></i> 156: 5 Pt 2 572-576<br/>

http://publicationslist.org/massimo.pinto/refid6 2008-02-05T17:03:51Z

PURPOSE: To analyse the currently existing methods to infer the extent of cellular DNA damage induced by ionizing radiation when the pulsed field gel electrophoresis (PFGE) technique is used. RESULTS AND CONCLUSIONS: PFGE is currently the method of choice for the measurement of radiation-induced double-strand breaks (dsb). For accurate determination of both the yields and distributions of breaks, ...<br/><br/>M Pinto, H C Newman, K M Prise, B D Michael (2000) <i>Int J Radiat Biol</i> <i></i> <i></i> 76: 6 741-748<br/>