Mattias Collin, Associate Professor 's Publications List

"This is an elegant study showing how effector functions of therapeutic monoclonal antibodies can be..." evaluation of: [Baruah K et al. Selective Deactivation of Serum IgG: A General Strategy for the Enhancement of Monoclonal Antibody Receptor Interactions. J Mol Biol. 2012 Apr 5; doi: 10.1016/j.jmb.2012.04.002].

2012-05-03T07:13:19Z

Collin M, Williams M (2012) Faculty of 1000, 30 Apr

Bacterial hydrolysis of host glycoproteins - powerful protein modification and efficient nutrient acquisition

2012-04-17T09:44:26Z

Glycoproteins are ubiquitous in nature and fundamental to most biological processes, including the human immune system. The glycoprotein carbohydrate moieties, or glycans, are very diverse in their structure and composition, and have major effects on the chemical, physical and biological properties of these glycoproteins. The hydrolysis of glycoprotein glycans by bacterial glycosidases can have dr...

Julia Garbe, Mattias Collin (2012) Journal of Innate Immunity : Published online Jan 3

Vorbildhafte Krankheitserreger

2012-01-26T12:23:47Z

Mattias Collin (2012) Frankfurter Allgemeine, Jan 17

IgG glycan hydrolysis by EndoS diminishes the pro-inflammatory properties of immune complexes from patients with SLE - a possible new treatment?

2012-04-17T09:44:35Z

OBJECTIVE.: Systemic lupus erythematosus (SLE) is an autoimmune disease with chronic or episodic inflammation in several organ systems, related to the presence of circulating and tissue-deposited immune complexes (ICs) which stimulate leukocytes through FcγRs with subsequent inflammation. Treatment with EndoS, an IgG glycan hydrolyzing bacterial enzyme from Streptococcus pyogenes, has shown benef...

Christian Lood, Maria Allhorn, Rolf Lood, Birgitta Gullstrand, Anders I Olin, Lars Rönnblom, Lennart Truedsson, Mattias Collin, Anders A Bengtsson (2012) Arthritis and Rheumatism : Published online March 5

Tolerance induction with T cell–dependent protein antigens induces regulatory sialylated IgGs

2012-04-17T09:44:48Z

BACKGROUND: Under inflammatory conditions, T cell-dependent (TD) protein antigens induce proinflammatory T- and B-cell responses. In contrast, tolerance induction by TD antigens without costimulation triggers the development of regulatory T cells. Under both conditions, IgG antibodies are generated, but whether they have different immunoregulatory functions remains elusive. OBJECTIVE: It was sh...

Carolin M Oefner, Andre Winkler, Constanze Hess, Alexandra K Lorenz, Vivien Holecska, Melanie Huxdorf, Tim Schommartz, Dominique Petzold, Josephine Bitterling, Anna-Lena Schoen, Alexander D Stoehr, Dana Vu Van, Yasemin Darcan-Nikolaisen, Veronique Blanchard, Inken Schmudde, Yves Laumonnier, Heike A Strover, Ahmed N Hegazy, Susanne Eiglmeier, Carolin T Schoen, Maria M M Mertes, Christoph Loddenkemper, Max Lohning, Peter Konig, Arnd Petersen, Elke O Luger, Mattias Collin, Jörg Köhl, Andreas Hutloff, Eckard Hamelmann, Markus Berger, Hedda Wardemann, and Marc Ehlers (2012) Journal of Allergy and Clinical Immunology : Published online April 16

"This is the first paper to actually show what has been suspected for a long..." Evaluation of: [Barb AW, Prestegard JH. NMR analysis demonstrates immunoglobulin G N-glycans are accessible and dynamic. Nat Chem Biol. 2011 Mar; 7(3):147-53; doi: 10.1038/nchembio.511]

2011-03-01T11:07:46Z

Mattias Collin, Marc A. Williams (2011) Faculty of 1000, 28 Feb

"This study represents the first direct example of hormonal regulation of IgG glycosylation. These findings..." Evaluation of: [Prados MB et al. Progesterone induces a switch in oligosaccharyltransferase isoform expression: Consequences on IgG N-glycosylation. Immunol Lett. 2011 Feb 12; doi: 10.1016/j.imlet.2011.01.017

2011-02-25T15:11:38Z

Mattias Collin, Marc A Williams (2011) Faculty of 1000, 21 Feb

"Nanobodies from furry giant protect against Salmonella toxin -- a surprising new development." Evaluation of: [Alzogaray V et al. Single-domain llama antibodies as specific intracellular inhibitors of SpvB, the actin ADP-ribosylating toxin of Salmonella typhimurium. FASEB J. 2011 Feb; 25(2):526-34; doi: 10.1096/fj.10-162958].

2011-02-25T15:11:53Z

Mattias Collin, Marc A Williams (2011) Faculty of 1000, 25 Feb

Prerequisites for research- The SULF research policy programme

2011-06-22T08:20:39Z

Elinor Edvardsson Stiwne, Mattias Collin, Iréne Bernhard, Anna Götlind, Gustav Malmborg, Hans Ruin, Ann Fritzell, and Christoph Bargholtz (2011) SULF:s skriftserie,issue XXXV, 38 pages, April 12

Study of the IgG endoglycosidase EndoS in group A streptococcal phagocyte resistance and virulence.

2012-02-17T12:56:56Z

BACKGROUND: The secreted enzyme EndoS, an endoglycosidase from Streptococcus pyogenes, hydrolyzes the N-linked glycan of the constant region of immunoglobulin G(IgG) heavy chain and renders the antibody unable to interact with Fc receptors and elicit effector functions. In this study we couple targeted allelic replacement mutagenesis and heterologous expression to elucidate the contribution of End...

Jonathan Sjögren, Cheryl Y.M. Okumura, Mattias Collin, Victor Nizet, Andrew Hollands (2011) BMC Microbiology 11: 1

Belöna universitet som förbättrar jämställdheten [Swedish]

2011-02-21T09:53:46Z

Pia Sandvik Wiklund, Maria Anvret, Mattias Collin, Erik Eriksson, Per Eriksson, Cristina Glad, Pia Höök, Ulf Mellström (2011) Dagens Nyheter, Jan 21

Krönika: Arma Mater

2011-06-22T08:24:40Z

Mattias Collin (2011) Universitetsläraren, 8:36

Characterization and genome sequencing of two Propionibacterium acnes phages displaying pseudolysogeny

2011-04-21T18:36:09Z

Background Propionibacterium acnes is a Gram positive rod inhabiting the human skin that also infects orthopaedic implants and is associated with acne vulgaris. Previously, one lytic bacteriophage, PA6, from P. acnes has been sequenced and partially characterized. We recently isolated several inducible phages from P. acnes classified as Siphoviruses based on morphology and partial genome sequen...

Rolf Lood and Mattias Collin (2011) BMC Genomics 12: 198

Propionibacterium acnes and its phages

2011-10-03T09:43:11Z

Microorganisms are everywhere! They can tolerate many diverse extreme environments, such as the human body. Even though many of us might associate the word microorganism with infections and disease, most are actually either harmless or even beneficial for us. Those commensals often fight off more dangerous bacteria and might also directly benefit their host. The human skin harbors several differen...

Rolf Lood (2011)

Bacteria can help us cure autoimmune disease

2012-01-18T11:22:59Z

Mattias Collin (2011) Irish Times, Dec 11

The membrane bound LRR lipoprotein Slr, and the cell wall-anchored M1 protein from Streptococcus pyogenes both interact with type I collagen.

2011-12-27T06:46:40Z

Streptococcus pyogenes is an important human pathogen and surface structures allow it to adhere to, colonize and invade the human host. Proteins containing leucine rich repeats (LRR) have been identified in mammals, viruses, archaea and several bacterial species. The LRRs are often involved in protein-protein interaction, are typically 20-30 amino acids long and the defining feature of the LRR mot...

Marta Bober, Matthias Mörgelin, Anders I Olin, Ulrich von Pawel-Rammingen, Mattias Collin (2011) PLoS One 6: e20345

Antibacterial activity of the contact and complement systems is blocked by SIC, a protein secreted by Streptococcus pyogenes

2011-04-29T08:11:15Z

Recent studies have shown that activation of the complement and contact systems results in the generation of antibacterial peptides. Streptococcus pyogenes, a major bacterial pathogen in humans, exists in more than one hundred different serotypes due to sequence variation in the surface-associated M protein. Cases of invasive and life-threatening S. pyogenes infections are commonly associated with...

Inga-Maria Frick, Oonagh Shannon, Per Åkesson, Matthias Mörgelin, Mattias Collin, Artur Schmidtchen and Lars Björck (2011) Journal of Biological Chemistry 286: 1331-40

Bacteria can help us cure autoimmune disease

2011-12-27T06:38:05Z

Mattias Collin (2011) El Paìs, Dec 16

Molecular and microscopical analysis of pathogenic streptococci - studies on surface proteins interacting with human cells and extracellular matrix

2011-10-03T09:42:37Z

Engelska Association to specific host tissue structures allows pathogenic bacteria to establish an infection and facilitates the spread within its host. Interactions between bacterial surface structures and human proteins might determine the outcome of the infection. Streptococcus pyogenes (S. pyogenes), is a human pathogen mostly causing localized infections of the skin and respiratory tract, bu...

Marta Bober (2011)

Cysteine proteinase SpeB from Streptococcus pyogenes – a potent modifier of immunologically important host and bacterial proteins

2012-01-03T10:27:56Z

Group A streptococcus (Streptococcus pyogenes) is an exclusively human pathogen that causes a wide spectrum of diseases ranging from pharyngitis, to impetigo, to toxic shock, to necrotizing fasciitis. The diversity of these disease states necessitates that S. pyogenes possess the ability to modulate both the innate and adaptive immune responses. SpeB, a cysteine proteinase, is the predominant secr...

Daniel C. Nelson, Julia Garbe, Mattias Collin (2011) Biological Chemistry 392: 1077-88

"This report presents novel clues about what makes B cells alter the functionally important glycans..." Evaluation of: [Wang J et al. Fc-glycosylation of IgG1 is modulated by B-cell stimuli. Mol Cell Proteomics. 2011 Mar 3; doi: 10.1074/mcp.M110.004655].

2011-04-26T08:24:56Z

Mattias Collin, Marc A. Williams (2011) Faculty of 1000, 26 Apr

"This study presents the first example of a Gram-negative enzyme system hydrolysing the functionally important..." Evaluation of: [Renzi F et al. The N-glycan glycoprotein deglycosylation complex (Gpd) from Capnocytophaga canimorsus deglycosylates human IgG. PLoS Pathogens. 2011; doi: 10.1371/journal.ppat.1002118]

2011-07-04T11:52:20Z

Mattias Collin, Marc A. Williams (2011) Faculty of 1000, 4 Jul

DEGLYCOSYLATED ANTIBODIES

2011-02-25T15:02:02Z

The present invention provides an antibody, or antibody fragment, with modified glycosylation for use in treating or preventing a disease or condition, where the antibody or fragment: (a) suppresses an inflammatory condition forming part of the disease or condition to be treated or prevented; and/or (b) displays increased efficacy and/or decreased side effects in comparision to treatment or preven...

Mattias Collin, Maria Allhorn, Rikard Holmdahl, Kutty Selva Nandakumar (2010) World Intellectual Property Organization

Midkine and pleiotrophin have bactericidal properties: preserved antibacterial activity in a family of heparin-binding growth factors during evolution.

2012-01-03T08:04:28Z

Antibacterial peptides of the innate immune system combat pathogenic microbes, but often have additional roles in promoting inflammation and as growth factors during tissue repair. Midkine (MK) and pleiotrophin (PTN) are the only two members of a family of heparin-binding growth factors. They show restricted expression during embryogenesis and are up-regulated in neoplasia. In addition, MK shows c...

Sara L Svensson, Mukesh Pasupuleti, Björn Walse, Martin Malmsten, Matthias Mörgelin, Camilla Sjögren, Anders I Olin, Mattias Collin, Artur Schmidtchen, Ruth Palmer, Arne Egesten (2010) Journal of Biological Chemistry 285: 21 16105-16115

The IgG-specific endoglycosidase EndoS inhibits both cellular and complement-mediated autoimmune hemolysis.

2012-01-03T07:37:57Z

EndoS from Streptococcus pyogenes is an immunomodulating enzyme that specifically hydrolyzes glycans from human immunoglobulin G and thereby affects antibody effector functions. Autoimmune hemolytic anemia is caused by antibody-mediated red blood cell (RBC) destruction and often resists treatment with corticosteroids that also cause frequent adverse effects. We show here that anti-RhD (anti-D) and...

Maria Allhorn, Juana G Briceño, Lucie Baudino, Christian Lood, Martin L Olsson, Shozo Izui, Mattias Collin (2010) Blood 115: 24 5080-5088

Synergism between a novel chimeric lysin and oxacillin protects against infection by methicillin-resistant Staphylococcus aureus.

2012-01-03T08:03:58Z

Staphylococcus aureus is the causative agent of several serious infectious diseases. The emergence of antibiotic-resistant S. aureus strains has resulted in significant treatment difficulties, intensifying the need for new antimicrobial agents. Toward this end, we have developed a novel chimeric bacteriophage (phage) lysin that is active against staphylococci, including methicillin-resistant S. au...

Anu Daniel, Chad Euler, Mattias Collin, Peter Chahales, Kenneth J Gorelick, Vincent A Fischetti (2010) Antimicrobial Agents and Chemotherapy 54: 4 1603-1612

IgG glycan hydrolysis attenuates ANCA-mediated glomerulonephritis.

2012-01-03T07:37:44Z

Anti-neutrophil cytoplasmic autoantibodies (ANCA) directed against myeloperoxidase (MPO) and proteinase 3 (Pr3) are considered pathogenic in ANCA-associated necrotizing and crescentic glomerulonephritis (NCGN) and vasculitis. Modulation of ANCA IgG glycosylation may potentially reduce its pathogenicity by abolishing Fc receptor-mediated activation of leukocytes and complement. Here, we investigate...

Mirjan M van Timmeren, Betty S van der Veen, Coen A Stegeman, Arjen H Petersen, Thomas Hellmark, Mattias Collin, Peter Heeringa (2010) Journal of the American Society for Nephrology 21: 7 1103-1114

Collagen VI is a subepithelial adhesive target for human respiratory tract pathogens.

2012-01-03T07:36:01Z

Bacterial engagement of specific host tissue structures can be a means of targeting a pathogen to a particular niche, establishing persistent infections and inducing invasion. In this context, primary adhesion is often the first crucial colonization step allowing pathogens to withstand the mechanical clearing mechanisms of the host. As a consequence, bacteria have evolved adhesins with the capacit...

Marta Bober, Charlotte Enochsson, Mattias Collin, Matthias Mörgelin (2010) Journal of Innate Immunity 2: 2 160-166

Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes.

2012-01-03T07:37:11Z

Anti-glomerular basement membrane (anti-GBM) disease often results in end-stage renal failure despite therapy with plasma exchange and immunosuppressive drugs. The newly discovered streptococcal enzymes IgG-degrading enzyme of S.pyogenes (IdeS) and endoglycosidase S (EndoS) act with remarkable specificity on circulating IgG. In this study, we investigate their ability in vivo to prevent damage med...

Rui Yang, Marielle A Otten, Thomas Hellmark, Mattias Collin, Lars Björck, Ming-Hui Zhao, Mohamed R Daha, Mårten Segelmark (2010) Nephrology Dialysis Transplantation 25: 8 2479-2486

SpeB of Streptococcus pyogenes differentially modulates antibacterial and receptor activating properties of human chemokines

2012-01-03T08:07:37Z

Background CXC chemokines are induced by inflammatory stimuli in epithelial cells and some, like MIG/CXCL9, IP–10/CXCL10 and I–TAC/CXCL11, are antibacterial for Streptococcus pyogenes. Methodology/Principal Findings SpeB from S. pyogenes degrades a wide range of chemokines (i.e. IP10/CXCL10, I-TAC/CXCL11, PF4/CXCL4, GROα/CXCL1, GROβ/CXCL2, GROγ/CXCL3, ENA78/CXCL5, GCP-2/CXCL6, NAP-2/CX...

A Egesten, A I Olin, H M Linge, M Yadav, M Mörgelin, A Karlsson, M Collin (2009) PLoS ONE 4: e4769

Bacterial Sensing and Signaling

2009-07-19T21:20:10Z

One of the keys to the development of novel anti-infective strategies Over the last fifteen years it has become increasingly obvious that bacteria are not as simple and solitary as once believed. Rather, an accumulating body of work shows that bacteria are highly complicated and social organisms, constantly sensing their surroundings and altering both their environments and behaviors to en...

(2009) 16:

Sugar free antibodies - the bacterial solution to autoimmunity?

2012-01-03T08:08:52Z

The enzyme EndoS from Streptococcus pyogenes is an immunomodulatory molecule hydrolyzing the conserved glycans in the effector part of immunoglobulin G (IgG). EndoS is remarkably specific for IgG, and hydrolysis has profound effects on IgG effector functions. EndoS pretreatment of IgG, or direct administration to animals with experimental antibody-mediated autoimmune diseases, inhibits development...

M Allhorn, M Collin (2009) Annals of the New York Academy of Sciences 1173: 664 - 669

METHOD AND KITS FOR DISSOCIATING FCGAMMA-RECEPTOR-IGG COMPLEXES AND FOR IGG PURIFICATION AND DETECTION

2009-05-26T13:41:16Z

The inventions provides methods and kits for the dissociation of Fcgamma-receptor-IgG complexes, and methods and kits for the isolation of IgG and Fc and Fab fragments of IgG.

M Allhorn, A I Olin, F Nimmerjahn, M Collin (2009) World Intellectual Property Organization

SufA - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation

2012-01-03T08:07:08Z

Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently and specifica...

C Karlsson, M Mörgelin, M Collin, R Lood, M Andersson, A Schmidtchen, L Björck, I M Frick (2009) Microbiology 155: 238-248

ANTIMICROBIAL POLYPEPTIDE

2009-08-21T12:25:35Z

The invention relates to a molecule comprising a first isolated peptide as shown in SEQ ID NO: 1 or part thereof or a peptide having at least 78 % homology to SEQ ID NO:1 conjugated to a second peptide wherein said second peptide is an amphipatic peptide with an alpha-helical structure or a linear cationic peptide and wherein said first and second peptide have a length of from about 5 to 100 amino...

Arne Egesten, Mattias Collin, Helena M. Linge (2009) World Intellectual Property Organization

Biofilm formation by Propionibacterium acnes is a characteristic of invasive isolates.

2012-01-03T07:38:34Z
Propionibacterium acnes is a common and probably underestimated cause of delayed joint prosthesis infection. Bacterial biofilm formation is central in the pathogenesis of infections related to foreign material, and P. acnes has been shown to form biofilm both in vitro and in vivo. Here, biofilm formation by 93 P. acnes isolates, either from invasive infections (n = 45) or from the skin of healthy ...

A Holmberg, R Lood, M Mörgelin, B Söderquist, E Holst, M Collin, B Christensson, M Rasmussen (2009) Clinical Microbiology and Infection 15: 8 787-795

The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma (Featured on the cover)

2012-01-03T08:10:17Z
The integrity of the urogenital tract against potentially invasive pathogens is important for the health of the individual, fertilization, and continuance of species. Antibiotic peptides with broad antimicrobial activity, among them chemokines, are part of the innate immune system. We investigated the presence of the antibacterial interferon (IFN)-dependent CXC chemokines, MIG/CXCL9, IP–10/CXCL1...

Helena M Linge, Mattias Collin, Alexander Giwercman, Johan Malm, Anders Bjartell, Arne Egesten (2008) Journal of Interferon and Cytokine Research 28: 191-196

From the cover: IgG glycan hydrolysis by a bacterial enzyme as a therapy against autoimmune conditions.

2012-01-03T07:40:20Z
EndoS from Streptococcus pyogenes efficiently hydrolyzes the functionally important and conserved N-linked glycan of IgG in human blood. Repeated i.v. administration of EndoS in rabbits completely hydrolyzes the glycans of the whole IgG pool, despite the generation of anti-EndoS antibodies. EndoS administration had no apparent effects on the health of the animals. EndoS hydrolysis of the IgG glyca...

Mattias Collin, Oonagh Shannon and Lars Björck (2008) Proceedings of the National Academy of Sciences USA 105: 4265-4270

Rapid DNA library construction for functional genomic and metagenomic screening

2012-01-03T07:39:00Z
A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning, and allows for inducible transcription in E. coli. As proof-of-principle, several anti-Bacillus lysins were cloned from bacteriophage genomes, and an aerolysin was cloned from a metagenomic sample.

Jonathan E Schmitz, Anu Daniel, Mattias Collin, Rymond Schuch, Vincent A Fischetti (2008) Applied and Environmental Microbiology 74: 5 1649-1652

Inducible Siphoviruses in superficial and deep tissue isolates of Propionibacterium acnes

2012-01-03T08:10:59Z
Background Propionibacterium acnes is a commensal of human skin but is also known to be involved in certain diseases, such as acne vulgaris and infections of orthopaedic implants. Treatment of these conditions is complicated by increased resistance to antibiotics and/or biofilm formation of P. acnes bacteria. P. acnes can be infected by bacteriophages, but until recently little has been known abo...

Rolf Lood, Matthias Mörgelin, Anna Holmberg, Magnus Rasmussen, Mattias Collin (2008) BMC Microbiology 8: 139

ENDOGLYCOSIDASE ENDOS FOR TREATING IMMUNOGLOBULIN G MEDIATED DISEASES

2009-05-26T13:30:19Z
The invention provides use of an EndoS polypeptide, or a polynucleotide encoding an EndoS polypeptide, in the manufacture of a medicament for the treatment or prevention of a disease or condition mediated by IgG antibodies.

L Björck, M Collin, A Olsén, R Holmdahl, K S Nandakumar, O Shannon (2008) World Intellectual Property Organization

EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity

2008-09-30T20:24:18Z
BACKGROUND: The endoglycosidase EndoS and the cysteine proteinase SpeB from the human pathogen Streptococcus pyogenes are functionally related in that they both hydrolyze IgG leading to impairment of opsonizing antibodies and thus enhance bacterial survival in human blood. In this study, we further investigated the relationship between EndoS and SpeB by examining their in vitro temporal production...

Maria Allhorn, Arne Olsén, Mattias Collin (2008) BMC Microbiology 8: 3

Constitutive expression of the antibacterial CXC chemokine GCP-2/CXCL6 by epithelial cells of the male reproductive tract

2012-01-03T08:11:14Z
The reproductive tract is continuously challenged by potential pathogens present in the environment. Therefore, robust host defense mechanisms are essential both for the health of the individual and for fertilization. Antibiotic innate immunity peptides possess broad antimicrobial activity. Recently, we found that the CXC chemokine, granulocyte chemotactic protein (GCP)-2/CXCL6, possesses antibact...

Mattias Collin, Helena M Linge, Anders Bjartell, Aleksander Giwercman, Johan Malm, Arne Egesten (2008) Journal of Reproductive Immunology 79: 37-43

Human IgG/Fc gamma R interactions are modulated by streptococcal IgG glycan hydrolysis.

2011-12-27T06:56:17Z
The human pathogen Streptococcus pyogenes produces an endoglycosidase, EndoS that hydrolyzes the chitobiose core of the asparagine-linked glycan on the heavy chain of human IgG. IgG-binding to Fc gamma receptors (Fc gamma R) on leukocytes triggers effector functions including phagocytosis, oxidative burst and the release of inflammatory mediators. The interactions between Fc gamma R and the Fc dom...

Maria Allhorn, Anders I Olin, Falk Nimmerjahn, Mattias Collin (2008) PLoS One 3: e1413

In vivo enzymatic modulation of IgG glycosylation inhibits autoimmune disease in an IgG subclass-dependent manner

2012-01-03T08:12:16Z
IgG antibodies are potent inducers of proinflammatory responses. During autoimmune diseases such as arthritis and systemic lupus erythematosus, IgG autoantibodies are responsible for the chronic inflammation and destruction of healthy tissues by cross-linking Fc receptors on innate immune effector cells. The sugar moiety attached to the asparagine-297 residue in the constant domain of the antibody...

Heike Albert, Mattias Collin, Diana Dudziak, Jeffrey V Ravetch, Falk Nimmerjahn (2008) Proceedings of the National Academy of Sciences USA 105: 39 15005-15009

The human CXC chemokine granulocyte chemotactic protein 2 (GCP-2)/CXCL6 possesses membrane-disrupting properties and is antibacterial.

2012-01-03T07:42:51Z
Granulocyte chemotactic protein 2 (GCP-2)/CXCL6 is a CXC chemokine expressed by macrophages and epithelial and mesenchymal cells during inflammation. Through binding and activation of its receptors (CXCR1 and CXCR2), it exerts neutrophil-activating and angiogenic activities. Here we show that GCP-2/CXCL6 itself is antibacterial. Antibacterial activity against gram-positive and gram-negative pathog...

Helena M Linge, Mattias Collin, Pontus Nordenfelt, Matthias Mörgelin, Martin Malmsten, Arne Egesten (2008) Antimicrobial Agents and Chemotherapy 52: 7 2599-2607

M1 protein of Streptococcus pyogenes increases production of the antibacterial CXC chemokine MIG/CXCL9 in pharyngeal epithelial cells.

2012-01-03T08:13:23Z
Streptococcus pyogenes adheres to epithelial cells of the human pharynx where it can cause pharyngitis. To counteract infection, inflamed epithelium produces peptide antibiotics, among them the CXC chemokine MIG/CXCL9. M protein is both a surface-associated and released virulence factor of S. pyogenes. Here, we show that soluble M1 protein enhances MIG gene expression and synthesis in IFN-gamma st...

Mette Eliasson, Inga-Maria Frick, Mattias Collin, Ole E Sørensen, Lars Björck, Arne Egesten (2007) Microbial Pathogenesis 43: 5-6 224-233

SufA a novel subtilisin-like serine proteinase of Finegoldia magna.

2012-01-03T08:14:17Z
Finegoldia magna is an anaerobic Gram-positive bacterium and commensal, which is also associated with clinically important conditions such as skin and soft tissue infections. This study describes a novel subtilisin-like extracellular serine proteinase of F. magna, denoted SufA (subtilase of Finegoldia magna), which is believed to be the first subtilase described among Gram-positive anaerobic cocci...

Christofer Karlsson, Marie-Louise Andersson, Mattias Collin, Artur Schmidtchen, Lars Björck, Inga-Maria Frick (2007) Microbiology 153: Pt 12 4208-4218

Kan man snacka bort representativ demokrati? [Swedish]

2011-02-21T10:03:12Z
Universitet och högskolor är unika myndigheter i Sverige genom att ha en demokratisk styrning med stark representation från verksamheten. I universitetsstyrelsen finns visserligen mer politiskt tillsatta ledamöter och representanter för det omgivande samhället, men bara ett steg längre ned på fakultetsnivå, som vår egen fakultet, är verksamhets- representationen i det närmaste total. ...

Mattias Collin (2007) NÄRV (Publication of the Medical Faculty, Lund University)

Endoglycosidase treatment abrogates IgG arthritogenicity: importance of IgG glycosylation in arthritis.

2012-01-03T07:45:09Z
The glycosylation status of IgG has been implicated in the pathology of rheumatoid arthritis. Earlier, we reported the identification of a novel secreted endo-beta-N-acetylglucosaminidase (EndoS), secreted by Streptococcus pyogenes that specifically hydrolyzes the beta-1,4-di-N-acetylchitobiose core of the asparagine-linked glycan of human IgG. Here, we analyzed the arthritogenicity of EndoS-treat...

Kutty Selva Nandakumar, Mattias Collin, Arne Olsén, Falk Nimmerjahn, Anna M Blom, Jeffrey V Ravetch, Rikard Holmdahl (2007) European Journal of Immunology 37: 10 2973-2982

Flexible alternative to rifampicin

2011-02-26T11:47:36Z
Mattias Collin (2005) Drug Discov. Today Drug Discovery Today, 7:530-531 10: 531

Direct antibacterial activity of complement peptide

2011-02-26T11:46:03Z
Mattias Collin (2005) Drug Discov. Today Drug Discovery Today, 10:75-77 10: 76

Universal vaccine and novel surface structure identified by Group B Streptococcus genomics

2011-02-26T11:49:36Z
Mattias Collin (2005) Drug Discov. Today Drug Discovery Today, 10:1201-1203 10: 1201-1202

Inhibition of essential enzymes in Mycobacterium tuberculosis

2011-02-26T11:48:34Z
Mattias Collin (2005) Drug Discov. Today Drug Discovery Today, 10:1073 10: 1073

Bacteriophage evolution : nature's own antimicrobial HTS

2011-02-28T09:59:26Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:287-290 9: 289

S. pyogenes vascular leakage can be inhibited by integrin antagonist

2011-02-28T10:00:19Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:338-340 9: 338

Staphylococcus aureus gets a helping van(d) from Enterococcus faecalis

2011-02-26T11:50:29Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:147-150 9: 150

Low antibody levels against cell wall-attached proteins of Streptococcus pyogenes predispose for severe invasive disease.

2012-01-03T07:48:04Z
Acute-phase serum samples from 70 patients with group A streptococcal (GAS) invasive disease were analyzed for IgG antibodies against 6 recently characterized GAS virulence factors (SclA, SclB, GRAB, MtsA, EndoS, and IdeS) and SpeB. Antibody levels against the cell wall-attached GAS antigens SclA, SclB, and GRAB were significantly lower in patients with severe invasive disease (streptococcal toxic...

Per Åkesson, Magnus Rasmussen, Ellen Mascini, Ulrich von Pawel-Rammingen, Robert Janulczyk, Mattias Collin, Arne Olsén, Eva Mattsson, Martin L Olsson, Lars Björck, Bertil Christensson (2004) Journal of Infectious Diseases 189: 5 797-804

Streptococcus pyogenes IL-8 protease is inhibited by pheromone

2011-02-28T10:01:59Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:420-422 9: 420-421

Don't forget about Streptococcus pyogenes! (comment on Microbes Infect. 5 (2003) 1329-1335).

2012-01-03T07:46:33Z
Mattias Collin (2004) Microbes Infect 6: 3

A novel secreted endoglycosidase from Enterococcus faecalis with activity on human immunoglobulin G and ribonuclease B.

2012-01-03T07:49:32Z
The human pathogen Enterococcus faecalis can degrade the N-linked glycans of human RNase B to acquire nutrients, but no gene or protein has been associated with this activity. We identified an 88-kDa secreted protein, endoglycosidase (Endo) E, which is most likely responsible for this activity. EndoE, encoded by ndoE, consists of an alpha-domain with a family 18 glycosyl hydrolase motif and a beta...

Mattias Collin, Vincent A Fischetti (2004) Journal of Biological Chemistry 279: 21 22558-22570

Caged and GRABbed: Streptococcus pyogenes protease degrades antimicrobial peptide

2011-02-28T10:05:54Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:1082-1084 9: 1082

Co-expressed attack and defense by group B streptococci

2011-02-28T10:05:18Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:941-943 9: 941

Fibronectin-binding in Streptococcus pyogenes: proteolytic regulation and virulence fine-tuning

2011-02-28T10:02:39Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:539-541 9: 539

Staphylococcal protein A induces inflammation in the airways

2011-02-28T10:04:16Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:722-724 9: 722

Novel Streptococcus pyogenes exotoxin disrupts cytoskeleton

2011-02-28T10:04:46Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:856-858 9: 856

MBL-deficiency and Staphylococcus aureus susceptibility

2011-02-28T10:03:11Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:621-622 9: 622

Cytolysin-mediated translocation and microdomain secretion in Streptococcus pyogenes

2012-01-03T08:17:41Z
Mattias Collin (2004) Drug Discov. Today Drug Discovery Today, 9:678-681 9: 680

Extracellular enzymes with immunomodulating activities: variations on a theme in Streptococcus pyogenes.

2012-01-03T07:49:58Z
Mattias Collin, Arne Olsén (2003) Infection and Immunity 71: 6 2983-2992

EndoS and SpeB from Streptococcus pyogenes inhibit immunoglobulin-mediated opsonophagocytosis.

2012-01-03T07:50:25Z
The human pathogen Streptococcus pyogenes primarily infects the upper respiratory tract and skin, but occasionally it disseminates and causes severe invasive disease with high mortality. This study revealed that the activity of extracellular EndoS, which hydrolyzes the functionally important N-linked oligosaccharides on opsonizing immunoglobulin G (IgG), contributes to increased survival of S. pyo...

Mattias Collin, Mikael D Svensson, Anders G Sjöholm, Jens C Jensenius, Ulf Sjöbring, Arne Olsén (2002) Infection and Immunity 70: 12 6646-6651

Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins.

2012-01-03T08:16:25Z
Streptococcus pyogenes secretes a specific immunoglobulin G (IgG)-protease, SpeB, as well as the IgG glycan-hydrolyzing enzyme EndoS. Here we show that SpeB also degrades IgA, IgM, IgD, and IgE. We also show that EndoS only hydrolyzes the glycan moiety on native but not denatured IgG. Thus, SpeB has a broad immunoglobulin-degrading activity, while EndoS is highly specific for IgG.

Mattias Collin, Arne Olsén (2001) Infection and Immunity 69: 11 7187-7189

Streptococcus pyogenes secreted enzymes interacting with the human host

2009-06-26T07:10:24Z
Streptococcus pyogenes is one of the most common bacteria infecting humans. One of the factors contributing to the disease-causing properties is the secreted streptococcal cysteine proteinase, SpeB, which degrades several host proteins in connective tissue and circulation. SpeB activity depends on the transformation from an inactive precursor, zymogen, into a mature proteinase. We show that the im...

M Collin (2001)

Identification of conditionally expressed genes in Streptococcus pyogenes using RNA fingerprinting.

2012-01-03T08:16:45Z
RNA fingerprinting using arbitrarily primed reverse transcription-polymerase chain reaction was employed on isolated RNA from Streptococcus pyogenes bacteria in order to identify genes that were regulated in response to environmental changes. When S. pyogenes was cultured under glucose-rich growth conditions a number of transcriptionally up-regulated products were identified, cloned and sequenced....

Mattias Collin, Arne Olsén (2001) FEMS Microbiology Letters 196: 2 123-127

EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG.

2012-01-03T07:51:48Z
Streptococcus pyogenes is an important human pathogen that selectively interacts with proteins involved in the humoral defense system, such as immunoglobulins and complement factors. In this report we show that S.pyogenes has the ability to hydrolyze the chitobiose core of the asparagine-linked glycan on immuno globulin G (IgG) when bacteria are grown in the presence of human plasma. This activity...

Mattias Collin, Arne Olsén (2001) EMBO Journal 20: 12 3046-3055

Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein.

2012-01-03T07:56:15Z
In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We a...

Mattias Collin, Arne Olsén (2000) Molecular Microbiology 36: 6 1306-1318

Protein H, an antiphagocytic surface protein in Streptococcus pyogenes.

2012-01-03T08:17:13Z
Surface-associated M protein is a major virulence factor in Streptococcus pyogenes which confers bacterial resistance to phagocytosis. However, many S. pyogenes strains also express additional structurally related so-called M-like proteins. The strain studied here is of the clinically important M1 serotype and expresses two structurally related surface proteins, the M1 protein and protein H. Mutan...

Britt-Marie Kihlberg, Mattias Collin, Arne Olsén, Lars Björck (1999) Infection and Immunity 67: 4 1708-1714

Streptococcal cysteine proteinase releases kinins: a novel virulence mechanism.

2012-01-03T08:00:03Z
Previous work has indicated a crucial role for the extracellular cysteine proteinase of Streptococcus pyogenes in the pathogenicity and virulence of this important human pathogen. Here we find that the purified streptococcal cysteine proteinase releases biologically active kinins from their purified precursor protein, H-kininogen, in vitro, and from kininogens present in the human plasma, ex vivo....

Heiko Herwald, Mattias Collin, Werner Müller-Esterl, Lars Björck (1996) Journal of Experimental Medicine 184: 2 665-673

Mattias Collin, Associate Professor 's Publications List

Bacterial hydrolysis of host glycoproteins - powerful protein modification and efficient nutrient acquisition

Vorbildhafte Krankheitserreger

IgG glycan hydrolysis by EndoS diminishes the pro-inflammatory properties of immune complexes from patients with SLE - a possible new treatment?

Tolerance induction with T cell–dependent protein antigens induces regulatory sialylated IgGs

"This is the first paper to actually show what has been suspected for a long..." Evaluation of: [Barb AW, Prestegard JH. NMR analysis demonstrates immunoglobulin G N-glycans are accessible and dynamic. Nat Chem Biol. 2011 Mar; 7(3):147-53; doi: 10.1038/nchembio.511]

Prerequisites for research- The SULF research policy programme

Study of the IgG endoglycosidase EndoS in group A streptococcal phagocyte resistance and virulence.

Belöna universitet som förbättrar jämställdheten [Swedish]

Krönika: Arma Mater

Characterization and genome sequencing of two Propionibacterium acnes phages displaying pseudolysogeny

Propionibacterium acnes and its phages

Bacteria can help us cure autoimmune disease

The membrane bound LRR lipoprotein Slr, and the cell wall-anchored M1 protein from Streptococcus pyogenes both interact with type I collagen.

Antibacterial activity of the contact and complement systems is blocked by SIC, a protein secreted by Streptococcus pyogenes

Bacteria can help us cure autoimmune disease

Molecular and microscopical analysis of pathogenic streptococci - studies on surface proteins interacting with human cells and extracellular matrix

Cysteine proteinase SpeB from Streptococcus pyogenes – a potent modifier of immunologically important host and bacterial proteins

"This report presents novel clues about what makes B cells alter the functionally important glycans..." Evaluation of: [Wang J et al. Fc-glycosylation of IgG1 is modulated by B-cell stimuli. Mol Cell Proteomics. 2011 Mar 3; doi: 10.1074/mcp.M110.004655].

DEGLYCOSYLATED ANTIBODIES

Midkine and pleiotrophin have bactericidal properties: preserved antibacterial activity in a family of heparin-binding growth factors during evolution.

The IgG-specific endoglycosidase EndoS inhibits both cellular and complement-mediated autoimmune hemolysis.

Synergism between a novel chimeric lysin and oxacillin protects against infection by methicillin-resistant Staphylococcus aureus.

IgG glycan hydrolysis attenuates ANCA-mediated glomerulonephritis.

Collagen VI is a subepithelial adhesive target for human respiratory tract pathogens.

Successful treatment of experimental glomerulonephritis with IdeS and EndoS, IgG-degrading streptococcal enzymes.

SpeB of Streptococcus pyogenes differentially modulates antibacterial and receptor activating properties of human chemokines

Bacterial Sensing and Signaling

Sugar free antibodies - the bacterial solution to autoimmunity?

METHOD AND KITS FOR DISSOCIATING FCGAMMA-RECEPTOR-IGG COMPLEXES AND FOR IGG PURIFICATION AND DETECTION

SufA - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation

ANTIMICROBIAL POLYPEPTIDE

Biofilm formation by Propionibacterium acnes is a characteristic of invasive isolates.

The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma (Featured on the cover)

From the cover: IgG glycan hydrolysis by a bacterial enzyme as a therapy against autoimmune conditions.

Rapid DNA library construction for functional genomic and metagenomic screening

Inducible Siphoviruses in superficial and deep tissue isolates of Propionibacterium acnes

ENDOGLYCOSIDASE ENDOS FOR TREATING IMMUNOGLOBULIN G MEDIATED DISEASES

EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity

Constitutive expression of the antibacterial CXC chemokine GCP-2/CXCL6 by epithelial cells of the male reproductive tract

Human IgG/Fc gamma R interactions are modulated by streptococcal IgG glycan hydrolysis.

In vivo enzymatic modulation of IgG glycosylation inhibits autoimmune disease in an IgG subclass-dependent manner

The human CXC chemokine granulocyte chemotactic protein 2 (GCP-2)/CXCL6 possesses membrane-disrupting properties and is antibacterial.

M1 protein of Streptococcus pyogenes increases production of the antibacterial CXC chemokine MIG/CXCL9 in pharyngeal epithelial cells.

SufA a novel subtilisin-like serine proteinase of Finegoldia magna.

Kan man snacka bort representativ demokrati? [Swedish]

Endoglycosidase treatment abrogates IgG arthritogenicity: importance of IgG glycosylation in arthritis.

Flexible alternative to rifampicin

Direct antibacterial activity of complement peptide

Universal vaccine and novel surface structure identified by Group B Streptococcus genomics

Inhibition of essential enzymes in Mycobacterium tuberculosis

Bacteriophage evolution : nature's own antimicrobial HTS

S. pyogenes vascular leakage can be inhibited by integrin antagonist

Staphylococcus aureus gets a helping van(d) from Enterococcus faecalis

Low antibody levels against cell wall-attached proteins of Streptococcus pyogenes predispose for severe invasive disease.

Streptococcus pyogenes IL-8 protease is inhibited by pheromone

Don't forget about Streptococcus pyogenes! (comment on Microbes Infect. 5 (2003) 1329-1335).

A novel secreted endoglycosidase from Enterococcus faecalis with activity on human immunoglobulin G and ribonuclease B.

Caged and GRABbed: Streptococcus pyogenes protease degrades antimicrobial peptide

Co-expressed attack and defense by group B streptococci

Fibronectin-binding in Streptococcus pyogenes: proteolytic regulation and virulence fine-tuning

Staphylococcal protein A induces inflammation in the airways

Novel Streptococcus pyogenes exotoxin disrupts cytoskeleton

MBL-deficiency and Staphylococcus aureus susceptibility

Cytolysin-mediated translocation and microdomain secretion in Streptococcus pyogenes

Extracellular enzymes with immunomodulating activities: variations on a theme in Streptococcus pyogenes.

EndoS and SpeB from Streptococcus pyogenes inhibit immunoglobulin-mediated opsonophagocytosis.

Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins.

Streptococcus pyogenes secreted enzymes interacting with the human host

Identification of conditionally expressed genes in Streptococcus pyogenes using RNA fingerprinting.

EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG.

Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein.

Protein H, an antiphagocytic surface protein in Streptococcus pyogenes.

Streptococcal cysteine proteinase releases kinins: a novel virulence mechanism.