Mattias Collin, Associate Professor 's Publications List

Synergism Between a Novel Chimeric Lysin and Oxacillin Protects Against Infection by Methicillin-Resistant Staphylococcus aureus

2010-01-19T21:22:59Z

Staphylococcus aureus is the causative agent of several serious infectious diseases. Emergence of antibiotic-resistant S. aureus strains has resulted in significant treatment difficulties, intensifying the need for new antimicrobial agents. Toward this end, we have developed a novel chimeric bacteriophage (phage) lysin that is active against staphylococci including methicillin-resistant S. aureus ...

Anu Daniel, Chad Euler, Mattias Collin, Peter Chahales, Kenneth Gorelick, and Vincent A. Fischetti (2010) Antimirobial Agents and Chemotherapy : Published online Jan 19, 2010

Sugar free antibodies - the bacterial solution to autoimmunity?

2009-09-02T14:25:50Z

The enzyme EndoS from Streptococcus pyogenes is an immunomodulatory molecule hydrolyzing the conserved glycans in the effector part of immunoglobulin G (IgG). EndoS is remarkably specific for IgG, and hydrolysis has profound effects on IgG effector functions. EndoS pretreatment of IgG, or direct administration to animals with experimental antibody-mediated autoimmune diseases, inhibits development...

M Allhorn, M Collin (2009) Annals of the New York Academy of Sciences 1173: 664 - 669

SpeB of Streptococcus pyogenes differentially modulates antibacterial and receptor activating properties of human chemokines

2009-05-26T12:56:58Z

Background CXC chemokines are induced by inflammatory stimuli in epithelial cells and some, like MIG/CXCL9, IP–10/CXCL10 and I–TAC/CXCL11, are antibacterial for Streptococcus pyogenes. Methodology/Principal Findings SpeB from S. pyogenes degrades a wide range of chemokines (i.e. IP10/CXCL10, I-TAC/CXCL11, PF4/CXCL4, GROα/CXCL1, GROβ/CXCL2, GROγ/CXCL3, ENA78/CXCL5, GCP-2/CXCL6, NAP-2/CX...

A Egesten, A I Olin, H M Linge, M Yadav, M Mörgelin, A Karlsson, M Collin (2009) PLoS ONE 4: e4769

SufA - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation

2009-01-01T10:35:53Z

Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently and specifica...

C Karlsson, M Mörgelin, M Collin, R Lood, M Andersson, A Schmidtchen, L Björck, I M Frick (2009) Microbiology 155: 238-248

Bacterial Sensing and Signaling

2009-07-19T21:20:10Z

One of the keys to the development of novel anti-infective strategies Over the last fifteen years it has become increasingly obvious that bacteria are not as simple and solitary as once believed. Rather, an accumulating body of work shows that bacteria are highly complicated and social organisms, constantly sensing their surroundings and altering both their environments and behaviors to en...

(2009) 16:

Biofilm formation by Propionibacterium acnes is a characteristic of invasive isolates

2009-04-27T09:19:04Z

Propionibacterium acnes is a common and probably underestimated cause of delayed joint prosthesis infection. Bacterial biofilm formation is central in the pathogenesis of infections related to foreign material, and P. acnes has been shown to form biofilm both in vitro and in vivo. Here, biofilm formation by 93 P. acnes isolates, either from invasive infections (n = 45) or from the skin of healthy ...

A Holmberg, R Lood, M Mörgelin, B Söderquist, E Holst, M Collin, B Christensson, M Rasmussen (2009) Clin Microbiol Infect : Published online

ANTIMICROBIAL POLYPEPTIDE

2009-08-21T12:25:35Z

The invention relates to a molecule comprising a first isolated peptide as shown in SEQ ID NO: 1 or part thereof or a peptide having at least 78 % homology to SEQ ID NO:1 conjugated to a second peptide wherein said second peptide is an amphipatic peptide with an alpha-helical structure or a linear cationic peptide and wherein said first and second peptide have a length of from about 5 to 100 amino...

Arne Egesten, Mattias Collin, Helena M. Linge (2009) World Intellectual Property Organization

Collagen VI is a subepithelial adhesive target for human respiratory tract pathogens

2009-08-03T06:42:32Z

Bacterial engagement of specific host tissue structures can be a means of targeting a pathogen to a particular niche, establishing persistent infections and inducing invasion. In this context, primary adhesion is often the first crucial colonization step allowing pathogens to withstand the mechanical clearing mechanisms of the host. As a consequence, bacteria have evolved adhesins with the capacit...

M Bober, C Enochsson, M Collin, M Mörgelin (2009) Journal of Innate Immunity : Published online Aug 1, 2009

METHOD AND KITS FOR DISSOCIATING FCGAMMA-RECEPTOR-IGG COMPLEXES AND FOR IGG PURIFICATION AND DETECTION

2009-05-26T13:41:16Z

The inventions provides methods and kits for the dissociation of Fcgamma-receptor-IgG complexes, and methods and kits for the isolation of IgG and Fc and Fab fragments of IgG.

M Allhorn, A I Olin, F Nimmerjahn, M Collin (2009) World Intellectual Property Organization

Rapid DNA library construction for functional genomic and metagenomic screening

2008-09-30T20:24:36Z

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning, and allows for inducible transcription in E. coli. As proof-of-principle, several anti-Bacillus lysins were cloned from bacteriophage genomes, and an aerolysin was cloned from a metagenomic sample.

Jonathan E Schmitz, Anu Daniel, Mattias Collin, Rymond Schuch, Vincent A Fischetti (2008) Appl Environ Microbiol 74: 5 1649-1652

From the cover: IgG glycan hydrolysis by a bacterial enzyme as a therapy against autoimmune conditions.

2008-09-30T20:24:52Z

EndoS from Streptococcus pyogenes efficiently hydrolyzes the functionally important and conserved N-linked glycan of IgG in human blood. Repeated i.v. administration of EndoS in rabbits completely hydrolyzes the glycans of the whole IgG pool, despite the generation of anti-EndoS antibodies. EndoS administration had no apparent effects on the health of the animals. EndoS hydrolysis of the IgG glyca...

Mattias Collin, Oonagh Shannon and Lars Björck (2008) Proc Natl Acad Sci U S A 105: 4265-4270

EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity

2008-09-30T20:24:18Z

BACKGROUND: The endoglycosidase EndoS and the cysteine proteinase SpeB from the human pathogen Streptococcus pyogenes are functionally related in that they both hydrolyze IgG leading to impairment of opsonizing antibodies and thus enhance bacterial survival in human blood. In this study, we further investigated the relationship between EndoS and SpeB by examining their in vitro temporal production...

Maria Allhorn, Arne Olsén, Mattias Collin (2008) BMC Microbiology 8: 3

The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma (Featured on the cover)

2008-09-30T20:26:21Z

The integrity of the urogenital tract against potentially invasive pathogens is important for the health of the individual, fertilization, and continuance of species. Antibiotic peptides with broad antimicrobial activity, among them chemokines, are part of the innate immune system. We investigated the presence of the antibacterial interferon (IFN)-dependent CXC chemokines, MIG/CXCL9, IP–10/CXCL1...

Helena M Linge, Mattias Collin, Alexander Giwercman, Johan Malm, Anders Bjartell, Arne Egesten (2008) Journal of Interferon and Cytokine Research 28: 191-196

Human IgG/Fc &gamma R Interactions Are Modulated by Streptococcal IgG Glycan Hydrolysis

2008-09-30T20:24:05Z

Background: The human pathogen Streptococcus pyogenes produces an endoglycosidase, EndoS that hydrolyzes the chitobiose core of the asparagine-linked glycan on the heavy chain of human IgG. IgG-binding to Fc gamma receptors (FcÎ³R) on leukocytes triggers effector functions including phagocytosis, oxidative burst and the release of inflammatory mediators. The interactions between FcÎ³R and the ...

Maria Allhorn, Anders I Olin, Falk Nimmerjahn, Mattias Collin (2008) PLoS ONE 3: 1 e1413

The human CXC chemokine granulocyte chemotactic protein 2 (GCP-2)/CXCL6 possesses membrane-disrupting properties and is antibacterial.

2008-09-22T13:45:50Z

Granulocyte chemotactic protein 2 (GCP-2)/CXCL6 is a CXC chemokine expressed by macrophages and epithelial and mesenchymal cells during inflammation. Through binding and activation of its receptors (CXCR1 and CXCR2), it exerts neutrophil-activating and angiogenic activities. Here we show that GCP-2/CXCL6 itself is antibacterial. Antibacterial activity against gram-positive and gram-negative pathog...

Helena M Linge, Mattias Collin, Pontus Nordenfelt, Matthias Mörgelin, Martin Malmsten, Arne Egesten (2008) Antimicrob Agents Chemother 52: 7 2599-2607

Inducible Siphoviruses in superficial and deep tissue isolates of Propionibacterium acnes

2008-09-30T20:26:43Z

Background Propionibacterium acnes is a commensal of human skin but is also known to be involved in certain diseases, such as acne vulgaris and infections of orthopaedic implants. Treatment of these conditions is complicated by increased resistance to antibiotics and/or biofilm formation of P. acnes bacteria. P. acnes can be infected by bacteriophages, but until recently little has been known abo...

Rolf Lood, Matthias Mörgelin, Anna Holmberg, Magnus Rasmussen, Mattias Collin (2008) BMC Microbiology 8: 139

In vivo enzymatic modulation of IgG glycosylation inhibits autoimmune disease in an IgG subclass-dependent manner

2008-09-30T20:32:29Z

IgG antibodies are potent inducers of proinflammatory responses. During autoimmune diseases such as arthritis and systemic lupus erythematosus, IgG autoantibodies are responsible for the chronic inflammation and destruction of healthy tissues by cross-linking Fc receptors on innate immune effector cells. The sugar moiety attached to the asparagine-297 residue in the constant domain of the antibody...

Heike Albert, Mattias Collin, Diana Dudziak, Jeffrey V Ravetch, Falk Nimmerjahn (2008) Proc. Natl. Acad. Sci. USA. 105: 39 15005-15009

ENDOGLYCOSIDASE ENDOS FOR TREATING IMMUNOGLOBULIN G MEDIATED DISEASES

2009-05-26T13:30:19Z

The invention provides use of an EndoS polypeptide, or a polynucleotide encoding an EndoS polypeptide, in the manufacture of a medicament for the treatment or prevention of a disease or condition mediated by IgG antibodies.

L Björck, M Collin, A Olsén, R Holmdahl, K S Nandakumar, O Shannon (2008) World Intellectual Property Organization

Constitutive expression of the antibacterial CXC chemokine GCP-2/CXCL6 by epithelial cells of the male reproductive tract

2008-09-30T20:27:01Z

The reproductive tract is continuously challenged by potential pathogens present in the environment. Therefore, robust host defense mechanisms are essential both for the health of the individual and for fertilization. Antibiotic innate immunity peptides possess broad antimicrobial activity. Recently, we found that the CXC chemokine, granulocyte chemotactic protein (GCP)-2/CXCL6, possesses antibact...

Mattias Collin, Helena M Linge, Anders Bjartell, Aleksander Giwercman, Johan Malm, Arne Egesten (2008) J. Reprod. Immunol. : In press

M1 protein of Streptococcus pyogenes increases production of the antibacterial CXC chemokine MIG/CXCL9 in pharyngeal epithelial cells.

2008-09-30T20:25:12Z

Streptococcus pyogenes adheres to epithelial cells of the human pharynx where it can cause pharyngitis. To counteract infection, inflamed epithelium produces peptide antibiotics, among them the CXC chemokine MIG/CXCL9. M protein is both a surface-associated and released virulence factor of S. pyogenes. Here, we show that soluble M1 protein enhances MIG gene expression and synthesis in IFN-gamma st...

Mette Eliasson, Inga-Maria Frick, Mattias Collin, Ole E Sørensen, Lars Björck, Arne Egesten (2007) Microb Pathog 43: 5-6 224-233

SufA a novel subtilisin-like serine proteinase of Finegoldia magna.

2008-09-30T20:25:26Z

Finegoldia magna is an anaerobic Gram-positive bacterium and commensal, which is also associated with clinically important conditions such as skin and soft tissue infections. This study describes a novel subtilisin-like extracellular serine proteinase of F. magna, denoted SufA (subtilase of Finegoldia magna), which is believed to be the first subtilase described among Gram-positive anaerobic cocci...

Christofer Karlsson, Marie-Louise Andersson, Mattias Collin, Artur Schmidtchen, Lars Björck, Inga-Maria Frick (2007) Microbiology 153: Pt 12 4208-4218

Endoglycosidase treatment abrogates IgG arthritogenicity: importance of IgG glycosylation in arthritis.

2008-09-30T20:25:57Z

The glycosylation status of IgG has been implicated in the pathology of rheumatoid arthritis. Earlier, we reported the identification of a novel secreted endo-beta-N-acetylglucosaminidase (EndoS), secreted by Streptococcus pyogenes that specifically hydrolyzes the beta-1,4-di-N-acetylchitobiose core of the asparagine-linked glycan of human IgG. Here, we analyzed the arthritogenicity of EndoS-treat...

Kutty Selva Nandakumar, Mattias Collin, Arne Olsén, Falk Nimmerjahn, Anna M Blom, Jeffrey V Ravetch, Rikard Holmdahl (2007) Eur J Immunol 37: 10 2973-2982

Flexible alternative to rifampicin

2007-10-03T14:01:51Z

Mattias Collin (2005) Drug Discov. Today 10: 531

Direct antibacterial activity of complement peptide

2007-10-03T14:02:14Z

Mattias Collin (2005) Drug Discov. Today 10: 76

Inhibition of essential enzymes in Mycobacterium tuberculosis

2008-06-03T13:34:50Z

Mattias Collin (2005) Drug Discov. Today 10: 1073

Universal vaccine and novel surface structure identified by Group B Streptococcus genomics

2008-06-03T13:34:19Z

Mattias Collin (2005) Drug Discov. Today 10: 1201-1202

S. pyogenes vascular leakage can be inhibited by integrin antagonist

2008-06-03T13:35:09Z

Mattias Collin (2004) Drug Discov. Today 9: 338

Streptococcus pyogenes IL-8 protease is inhibited by pheromone

2008-06-03T13:41:11Z

Mattias Collin (2004) Drug Discov. Today 9: 420-421

Staphylococcus aureus gets a helping van(d) from Enterococcus faecalis

2008-06-03T13:35:52Z

Mattias Collin (2004) Drug Discov. Today 9: 150

A novel secreted endoglycosidase from Enterococcus faecalis with activity on human immunoglobulin G and ribonuclease B.

2008-09-30T20:27:45Z

The human pathogen Enterococcus faecalis can degrade the N-linked glycans of human RNase B to acquire nutrients, but no gene or protein has been associated with this activity. We identified an 88-kDa secreted protein, endoglycosidase (Endo) E, which is most likely responsible for this activity. EndoE, encoded by ndoE, consists of an alpha-domain with a family 18 glycosyl hydrolase motif and a beta...

Mattias Collin, Vincent A Fischetti (2004) J Biol Chem 279: 21 22558-22570

Don't forget about Streptococcus pyogenes! (comment on Microbes Infect. 5 (2003) 1329-1335).

2008-06-03T13:40:07Z

Mattias Collin (2004) Microbes Infect 6: 3

Low antibody levels against cell wall-attached proteins of Streptococcus pyogenes predispose for severe invasive disease.

2008-09-30T20:27:27Z

Acute-phase serum samples from 70 patients with group A streptococcal (GAS) invasive disease were analyzed for IgG antibodies against 6 recently characterized GAS virulence factors (SclA, SclB, GRAB, MtsA, EndoS, and IdeS) and SpeB. Antibody levels against the cell wall-attached GAS antigens SclA, SclB, and GRAB were significantly lower in patients with severe invasive disease (streptococcal toxic...

Per Åkesson, Magnus Rasmussen, Ellen Mascini, Ulrich von Pawel-Rammingen, Robert Janulczyk, Mattias Collin, Arne Olsén, Eva Mattsson, Martin L Olsson, Lars Björck, Bertil Christensson (2004) J Infect Dis 189: 5 797-804

Fibronectin-binding in Streptococcus pyogenes: proteolytic regulation and virulence fine-tuning

2008-06-03T13:40:47Z

Mattias Collin (2004) Drug Discov. Today 9: 539

Bacteriophage evolution : nature's own antimicrobial HTS

2007-10-03T14:04:22Z

Mattias Collin (2004) Drug Discov. Today 9: 289

Caged and GRABbed: Streptococcus pyogenes protease degrades antimicrobial peptide

2008-06-03T13:39:45Z

Mattias Collin (2004) Drug Discov. Today 9: 1082

MBL-deficiency and Staphylococcus aureus susceptibility

2008-06-03T13:40:26Z

Mattias Collin (2004) Drug Discov. Today 9: 622

Novel Streptococcus pyogenes exotoxin disrupts cytoskeleton

2008-06-03T13:37:06Z

Mattias Collin (2004) Drug Discov. Today 9: 856

Co-expressed attack and defense by group B streptococci

2007-10-03T14:02:47Z

Mattias Collin (2004) Drug Discov. Today 9: 941

Cytolysin-mediated translocation and microdomain secretion in Streptococcus pyogenes

2007-10-03T14:04:32Z

Mattias Collin (2004) Drug Discov. Today 9: 680

Staphylococcal protein A induces inflammation in the airways

2007-10-03T14:03:16Z

Mattias Collin (2004) Drug Discov. Today 9: 722

Extracellular enzymes with immunomodulating activities: variations on a theme in Streptococcus pyogenes.

2008-09-30T20:28:04Z

Mattias Collin, Arne Olsén (2003) Infect Immun 71: 6 2983-2992

EndoS and SpeB from Streptococcus pyogenes inhibit immunoglobulin-mediated opsonophagocytosis.

2008-09-30T20:28:21Z

The human pathogen Streptococcus pyogenes primarily infects the upper respiratory tract and skin, but occasionally it disseminates and causes severe invasive disease with high mortality. This study revealed that the activity of extracellular EndoS, which hydrolyzes the functionally important N-linked oligosaccharides on opsonizing immunoglobulin G (IgG), contributes to increased survival of S. pyo...

Mattias Collin, Mikael D Svensson, Anders G Sjöholm, Jens C Jensenius, Ulf Sjöbring, Arne Olsén (2002) Infect Immun 70: 12 6646-6651

Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins.

2008-09-30T20:28:44Z

Streptococcus pyogenes secretes a specific immunoglobulin G (IgG)-protease, SpeB, as well as the IgG glycan-hydrolyzing enzyme EndoS. Here we show that SpeB also degrades IgA, IgM, IgD, and IgE. We also show that EndoS only hydrolyzes the glycan moiety on native but not denatured IgG. Thus, SpeB has a broad immunoglobulin-degrading activity, while EndoS is highly specific for IgG.

Mattias Collin, Arne Olsén (2001) Infect Immun 69: 11 7187-7189

Streptococcus pyogenes secreted enzymes interacting with the human host

2009-06-26T07:10:24Z

Streptococcus pyogenes is one of the most common bacteria infecting humans. One of the factors contributing to the disease-causing properties is the secreted streptococcal cysteine proteinase, SpeB, which degrades several host proteins in connective tissue and circulation. SpeB activity depends on the transformation from an inactive precursor, zymogen, into a mature proteinase. We show that the im...

M Collin (2001)

Identification of conditionally expressed genes in Streptococcus pyogenes using RNA fingerprinting.

2008-09-30T20:29:38Z

RNA fingerprinting using arbitrarily primed reverse transcription-polymerase chain reaction was employed on isolated RNA from Streptococcus pyogenes bacteria in order to identify genes that were regulated in response to environmental changes. When S. pyogenes was cultured under glucose-rich growth conditions a number of transcriptionally up-regulated products were identified, cloned and sequenced....

Mattias Collin, Arne Olsén (2001) FEMS Microbiol Lett 196: 2 123-127

EndoS, a novel secreted protein from Streptococcus pyogenes with endoglycosidase activity on human IgG.

2008-09-30T20:29:15Z

Streptococcus pyogenes is an important human pathogen that selectively interacts with proteins involved in the humoral defense system, such as immunoglobulins and complement factors. In this report we show that S.pyogenes has the ability to hydrolyze the chitobiose core of the asparagine-linked glycan on immuno globulin G (IgG) when bacteria are grown in the presence of human plasma. This activity...

Mattias Collin, Arne Olsén (2001) EMBO J 20: 12 3046-3055

Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein.

2008-09-30T20:29:52Z

In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We a...

Mattias Collin, Arne Olsén (2000) Mol Microbiol 36: 6 1306-1318

Protein H, an antiphagocytic surface protein in Streptococcus pyogenes.

2008-09-30T20:30:31Z

Surface-associated M protein is a major virulence factor in Streptococcus pyogenes which confers bacterial resistance to phagocytosis. However, many S. pyogenes strains also express additional structurally related so-called M-like proteins. The strain studied here is of the clinically important M1 serotype and expresses two structurally related surface proteins, the M1 protein and protein H. Mutan...

Britt-Marie Kihlberg, Mattias Collin, Arne Olsén, Lars Björck (1999) Infect Immun 67: 4 1708-1714

Streptococcal cysteine proteinase releases kinins: a novel virulence mechanism.

2008-09-30T20:31:07Z

Previous work has indicated a crucial role for the extracellular cysteine proteinase of Streptococcus pyogenes in the pathogenicity and virulence of this important human pathogen. Here we find that the purified streptococcal cysteine proteinase releases biologically active kinins from their purified precursor protein, H-kininogen, in vitro, and from kininogens present in the human plasma, ex vivo....

Heiko Herwald, Mattias Collin, Werner Müller-Esterl, Lars Björck (1996) J Exp Med 184: 2 665-673