http://publicationslist.org/data/nicolas.bayan/atom.xml Nicolas Bayanhttp://publicationslist.org/nicolas.bayan$basepathfavicon.icoRecent additions to Nicolas Bayan's PublicationsList.org pagehttp://publicationslist.org/publications.png2010-11-23T13:14:26Z http://publicationslist.org/nicolas.bayan/refid26 2010-07-14T11:36:04Z

Roland Bou Raad, Xavier Méniche, Celia de Sousa-d’Auria, Mohamed Chami, Christophe Salmeron, Marielle Tropis, Cecile Labarre, Mamadou Daffé, Christine Houssin and Nicolas Bayan (2010) <i>J Bacteriology</i> 192: 11 2691-2700<br/>

http://publicationslist.org/nicolas.bayan/refid27 2010-07-21T06:52:32Z

Emilie Huc, Xavier Meniche, Roland Benz, Nicolas Bayan, Alexandre Ghazi, Marielle Tropis, Mamadou Daffé (2010) <i>J of Biological Chemistry</i> 285: 21908-21912<br/>

http://publicationslist.org/nicolas.bayan/refid28 2010-10-16T13:55:04Z

The mechanosensitive channel MscL of the plasma membrane of bacteria is a homopentamer involved in the protection of cells during osmotic downshock. The MscL protein, a polypeptide of 136 residues, was recently shown to require YidC to be inserted in the inner membrane of E. coli. The insertase YidC is a component of an insertion pathway conserved in bacteria, mitochondria and chloroplasts. MscL i...<br/><br/>Catherine Berrier, Ingrid Guilvout, Nicolas Bayan, Kyu-Ho Park, Agnes Mesneau, Mohamed Chami, Anthony P Pugsley, Alexandre Ghazi (2010) <i>Biochim Biophys Acta</i> <i></i> <i></i> : <br/>

http://publicationslist.org/nicolas.bayan/refid25 2010-03-12T11:48:12Z

Corynebacterineae are gram-positive bacteria that possess a true outer membrane composed of mycolic acids and other lipids. Little is known concerning the modulation of mycolic acid composition and content in response to changes in the bacterial environment, especially temperature variations. To address this question, we investigated the function of the Rv3802c gene, a gene conserved in Corynebact...<br/><br/>Xavier Meniche, Cécile Labarre, Célia de Sousa-d'Auria, Emilie Huc, Françoise Laval, Marielle Tropis, Nicolas Bayan, Damien Portevin, Christophe Guilhot, Mamadou Daffé, Christine Houssin (2009) <i>J Bacteriol</i> <i></i> <i></i> 191: 23 7323-7332<br/>

http://publicationslist.org/nicolas.bayan/refid23 2009-08-07T10:26:41Z

Synthesis of the Klebsiella oxytoca outer membrane secretin PulD, or its membrane-associated core domain, in a liposome-supplemented Escherichia coli in vitro transcription-translation system resulted in the formation of multimers that appeared as typical dodecameric secretin rings when examined by negative-stain electron microscopy. Cryo-electron microscopy of unstained liposomes and differential...<br/><br/>Ingrid Guilvout, Mohamed Chami, Catherine Berrier, Alexandre Ghazi, Andreas Engel, Anthony P Pugsley, Nicolas Bayan (2008) <i>J Mol Biol</i> 382: 1 13-23<br/>

http://publicationslist.org/nicolas.bayan/refid4 2008-05-25T12:51:21Z

Secretins are a unique class of bacterial multimeric outer membrane proteins that probably differ considerably from other, less complex outer membrane proteins in their overall structure and organization, and in their requirements for outer membrane targeting and assembly factors. In this MicroCommentary, we discuss these differences with respect to the role of a specific class of lipoproteins, of...<br/><br/>Nicolas Bayan, Ingrid Guilvout, Anthony P Pugsley (2006) <i>Mol Microbiol</i> <i></i> <i></i> 60: 1 1-4<br/>

http://publicationslist.org/nicolas.bayan/refid3 2008-05-25T12:51:21Z

Dodecamerization and insertion of the outer membrane secretin PulD is entirely determined by the C-terminal half of the polypeptide (PulD-CS). In the absence of its cognate chaperone PulS, PulD-CS and PulD mislocalize to the inner membrane, from which they are extractable with detergents but not urea. Electron microscopy of PulD-CS purified from the inner membrane revealed apparently normal dodeca...<br/><br/>Ingrid Guilvout, Mohamed Chami, Andreas Engel, Anthony P Pugsley, Nicolas Bayan (2006) <i>EMBO J</i> <i></i> <i></i> 25: 22 5241-5249<br/>

http://publicationslist.org/nicolas.bayan/refid5 2008-05-25T12:51:21Z

Limited proteolysis, secondary structure and biochemical analyses, mass spectrometry, and mass measurements by scanning transmission electron microscopy were combined with cryo-electron microscopy to generate a three-dimensional model of the homomultimeric complex formed by the outer membrane secretin PulD, an essential channel-forming component of the type II secretion system from Klebsiella oxyt...<br/><br/>Mohamed Chami, Ingrid Guilvout, Marco Gregorini, Hervé W Rémigy, Shirley A Müller, Marielle Valerio, Andreas Engel, Anthony P Pugsley, Nicolas Bayan (2005) <i>J Biol Chem</i> <i></i> <i></i> 280: 45 37732-37741<br/>

http://publicationslist.org/nicolas.bayan/refid7 2008-05-25T12:51:21Z

Corynebacteria belong to a distinct Gram-positive group of bacteria including mycobacteria and nocardia, which are characterized by the presence of mycolic acids in their cell wall. These bacteria share the property of having an unusual cell envelope structural organization close to Gram-negative bacteria. In addition to the inner membrane, the cell envelope is constituted of a thick arabinogalact...<br/><br/>N Bayan, C Houssin, M Chami, G Leblon (2003) <i>J Biotechnol</i> <i></i> <i></i> 104: 1-3 55-67<br/>

http://publicationslist.org/nicolas.bayan/refid9 2008-05-25T12:51:21Z

Vesicles consisting of pure trehalose dicorynomycolate (TDCM), the corynebacterial analog of the most studied mycobacterial glycolipid 'cord factor', were isolated from Corynebacterium glutamicum cells by mild detergent treatment; these induced in vivo a macrophage priming similar to that obtained with mycobacterial-derived trehalose dimycolate. In vitro, both TDCM and bacterial lipopolysaccharide...<br/><br/>Mohamed Chami, Karine Andréau, Anne Lemassu, Jean-François Petit, Christine Houssin, Virginie Puech, Nicolas Bayan, Richard Chaby, Mamadou Daffé (2002) <i>FEMS Immunol Med Microbiol</i> <i></i> <i></i> 32: 2 141-147<br/>

http://publicationslist.org/nicolas.bayan/refid8 2008-05-25T12:51:21Z

Corynebacteria are Gram-positive bacteria with a very peculiar cell envelope structure as it is constituted of an inner membrane and an outer membrane-like structure. Protein secretion in Corynebacterium glutamicum was studied in vivo, using the S-layer protein PS2 as a model. We show that different variants of PS2 protein are exported through the whole cell envelope with a half-life ranging betwe...<br/><br/>C Houssin, D T Nguyen, G Leblon, N Bayan (2002) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 217: 1 71-79<br/>

http://publicationslist.org/nicolas.bayan/refid12 2008-05-25T12:51:21Z

When expressed in Escherichia coli, the 15 Klebsiella oxytoca pul genes that encode the so-called Pul secreton or type II secretion machinery promote pullulanase secretion and the assembly of one of the secreton components, PulG, into pili. Besides these pul genes, efficient pullulanase secretion also requires the host dsbA gene, encoding a periplasmic disulfide oxidoreductase, independently of di...<br/><br/>A P Pugsley, N Bayan, N Sauvonnet (2001) <i>J Bacteriol</i> <i></i> <i></i> 183: 4 1312-1319<br/>

http://publicationslist.org/nicolas.bayan/refid10 2008-05-25T12:51:21Z

PS1 is a major exported protein of Corynebacterium glutamicum homologous to mycobacterial antigen 85. It is largely associated with the mycolic acid-containing cell wall and acts as a mycoloyl transferase. The transport of PS1 to the cell wall is slow and occurs through two energetically distinct steps: the first one, which includes processing by signal peptidase, is rapid and inhibited by sodium ...<br/><br/>D T Nguyen, C Houssin, N Bayan (2001) <i>FEMS Microbiol Lett</i> <i></i> <i></i> 201: 2 145-150<br/>

http://publicationslist.org/nicolas.bayan/refid11 2008-05-25T12:51:21Z

With the recent success of the heterologous expression of mycobacterial antigens in corynebacteria, in addition to the importance of these bacteria in biotechnology and medicine, a better understanding of the structure of their cell envelopes was needed. A combination of molecular compositional analysis, ultrastructural appearance and freeze-etch electron microscopy study was used to arrive at a c...<br/><br/>V Puech, M Chami, A Lemassu, M A Lanéelle, B Schiffler, P Gounon, N Bayan, R Benz, M Daffé (2001) <i>Microbiology</i> <i></i> <i></i> 147: Pt 5 1365-1382<br/>

http://publicationslist.org/nicolas.bayan/refid13 2008-05-25T12:52:19Z

Mycolic acids, long-chain (C70-C90) alpha-alkyl, beta-hydroxy fatty acids, are characteristic cell envelope components of mycobacteria; similar but shorter-chain substances occur in corynebacteria and related taxa. These compounds apparently play an important role in the physiology of these bacteria. The deduced N-terminal region of PS1, one of the two major secreted proteins of Corynebacterium gl...<br/><br/>V Puech, N Bayan, K Salim, G Leblon, M Daffé (2000) <i>Mol Microbiol</i> <i></i> <i></i> 35: 5 1026-1041<br/>

http://publicationslist.org/nicolas.bayan/refid14 2008-05-25T12:52:19Z

Three strains of Corynebacterium producing various amounts of PS2 S-layer protein were studied. For all strains, more PS2 was produced if the bacteria were grown in minimal medium supplemented with lactate than if they were grown in minimal medium supplemented with glucose. The consumption of substrate and PS2 production was studied in cultures with mixed carbon sources. It was found that the inhi...<br/><br/>E Soual-Hoebeke, C de Sousa-D'Auria, M Chami, M F Baucher, A Guyonvarch, N Bayan, K Salim, G Leblon (1999) <i>Microbiology</i> <i></i> <i></i> 145 ( Pt 12): 3399-3408<br/>

http://publicationslist.org/nicolas.bayan/refid15 2008-05-25T12:52:19Z

In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is considerable variety in the S-layer composition, whic...<br/><br/>H Bahl, H Scholz, N Bayan, M Chami, G Leblon, T Gulik-Krzywicki, E Shechter, A Fouet, S Mesnage, E Tosi-Couture, P Gounon, M Mock, E Conway de Macario, A J Macario, L A Fernández-Herrero, G Olabarría, J Berenguer, M J Blaser, B Kuen, W Lubitz, M Sára, P H Pouwels, C P Kolen, H J Boot, S Resch (1997) <i>FEMS Microbiol Rev</i> <i></i> <i></i> 20: 1-2 47-98<br/>

http://publicationslist.org/nicolas.bayan/refid16 2008-05-25T12:52:19Z

PS2 is the S-layer protein of Corynebacterium glutamicum. The S-layer may be detached from the cell as organized sheets by detergents at room temperature. The solubilization of PS2 in the form of monomers requires detergent treatment at high temperature (70 degrees C), conditions under which the protein is denatured. Treatment of the cells with proteinase K or trypsin results in the detachment of ...<br/><br/>M Chami, N Bayan, J L Peyret, T Gulik-Krzywicki, G Leblon, E Shechter (1997) <i>Mol Microbiol</i> <i></i> <i></i> 23: 3 483-492<br/>

http://publicationslist.org/nicolas.bayan/refid17 2008-05-25T12:52:19Z

The cell surface of Corynebacterium glutamicum grown on solid medium was totally covered with a highly ordered, hexagonal surface layer. Also, freeze-fracture revealed two fracture surfaces which were totally covered with ordered arrays displaying an hexagonal arrangement and the same unit cell dimension as the surface layer. The ordered arrays on the concave fracture surface, closest to the cell ...<br/><br/>M Chami, N Bayan, J Dedieu, G Leblon, E Shechter, T Gulik-Krzywicki (1995) <i>Biol Cell</i> <i></i> <i></i> 83: 2-3 219-229<br/>

http://publicationslist.org/nicolas.bayan/refid18 2008-05-25T12:52:19Z

We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable to protons. These membrane vesicles may be the starting point for in vitro translo...<br/><br/>S Schrempp, N Bayan, E Shechter (1994) <i>FEBS Lett</i> <i></i> <i></i> 356: 1 104-108<br/>

http://publicationslist.org/nicolas.bayan/refid20 2008-05-25T12:52:19Z

PS1 is a protein translocated across the cytoplasmic membrane of Corynebacterium glutamicum, a Gram-positive bacterium. Western blots of whole cell extracts showed the presence of two bands associated with the mature and the precursor forms. Addition of chloramphenicol led to the disappearance of the precursor form while dissipation of the protonmotive force (delta microH) prior to the addition of...<br/><br/>N Bayan, S Schrempp, G Joliff, G Leblon, E Shechter (1993) <i>Biochim Biophys Acta</i> <i></i> <i></i> 1146: 1 97-105<br/>

http://publicationslist.org/nicolas.bayan/refid19 2008-05-25T12:52:19Z

PS2 is one of two major proteins detected in the culture media of various Corynebacterium glutamicum strains. The coding and promoter regions of the cspB gene encoding PS2 were cloned in lambda gt11 using polyclonal antibodies raised against PS2 for screening. Expression of the cspB gene in Escherichia coli led to the production of a major anti-PS2 labelled peptide of 63,000 Da, corresponding pres...<br/><br/>J L Peyret, N Bayan, G Joliff, T Gulik-Krzywicki, L Mathieu, E Schechter, G Leblon (1993) <i>Mol Microbiol</i> <i></i> <i></i> 9: 1 97-109<br/>

http://publicationslist.org/nicolas.bayan/refid22 2008-05-25T12:52:19Z

Acyl Carrier Protein (ACP) is a small acidic protein which interacts with the various enzymes implicated in the biosynthesis of fatty acids in E. coli. It also interacts with the inner membrane proteins implicated in the biosynthesis of phospholipids. Samples of radioactive ACP were prepared with high specific activities and bearing photoactivable aryl azide derivatives. Two photoactivable reagent...<br/><br/>N Bayan, H Thérisod (1992) <i>Biochim Biophys Acta</i> <i></i> <i></i> 1123: 2 191-197<br/>

http://publicationslist.org/nicolas.bayan/refid21 2008-05-25T12:52:19Z

Two proteins, PS1 and PS2, were detected in the culture medium of Corynebacterium glutamicum and are the major proteins secreted by this bacterium. No enzymatic activity was identified for either of the two proteins. Immunologically cross-reacting proteins were found in a variety of C. glutamicum strains but not in the coryneform Arthrobacter aureus. The gene encoding PS1, csp1, was cloned in lamb...<br/><br/>G Joliff, L Mathieu, V Hahn, N Bayan, F Duchiron, M Renaud, E Schechter, G Leblon (1992) <i>Mol Microbiol</i> <i></i> <i></i> 6: 16 2349-2362<br/>

http://publicationslist.org/nicolas.bayan/refid1 2008-05-25T12:50:43Z

We [(1989) FEBS Lett., in press] have previously shown that membrane vesicles from Escherichia coli contain protein-binding sites for the acyl carrier protein (ACP). We report now that membrane vesicles prepared from a strain amplified for glycerol-3-phosphate acyltransferase (GPAT) contain a higher number of ACP-binding sites than the membrane vesicles prepared from a wild type strain. In additio...<br/><br/>N Bayan, H Thérisod (1989) <i>FEBS Lett</i> <i></i> <i></i> 255: 2 330-334<br/>

http://publicationslist.org/nicolas.bayan/refid2 2008-05-25T12:50:43Z

We report that membrane vesicles of Escherichia coli contain protein-binding sites for acyl carrier protein. Scatchard analysis of the binding indicates a dissociation constant around 0.35 micrometers and a maximum number of protein-binding sites around 50 pmol per mg of membrane protein. Binding is on the inner membrane while the outer membrane is devoid of binding sites. These results are consis...<br/><br/>N Bayan, H Therisod (1989) <i>FEBS Lett</i> <i></i> <i></i> 253: 1-2 221-225<br/>