Rolf Lood's Publications List

Novel phage lysin capable of killing the multidrug-resistant gram-negative bacterium Acinetobacter baumannii in a mouse bacteremia model.

2015-05-23T14:46:06Z

Acinetobacter baumannii, a Gram-negative multidrug-resistant (MDR) bacterium, is now recognized as one of the more common nosocomial pathogens. Because most clinical isolates are found to be multidrug resistant, alternative therapies need to be developed to control this pathogen. We constructed a bacteriophage genomic library based on prophages induced from 13 A. baumannii strains and screened it ...

Rolf Lood, Benjamin Y Winer, Adam J Pelzek, Roberto Diez-Martinez, Mya Thandar, Chad W Euler, Raymond Schuch, Vincent A Fischetti (2015) Antimicrobial agents and chemotherapy 59: 4 1983-1991

A highly active and negatively charged Streptococcus pyogenes lysin with a rare D-alanyl-L-alanine endopeptidase activity protects mice against streptococcal bacteremia.

2015-05-23T14:46:39Z

Bacteriophage endolysins have shown great efficacy in killing Gram-positive bacteria. PlyC, a group C streptococcal phage lysin, represents the most efficient lysin characterized to date, with a remarkably high specificity against different streptococcal species, including the important pathogen Streptococcus pyogenes. However, PlyC is a unique lysin, in terms of both its high activity and structu...

Rolf Lood, Assaf Raz, Henrik Molina, Chad W Euler, Vincent A Fischetti (2014) Antimicrobial agents and chemotherapy 58: 6 3073-3084

Activity assay for bacteriophage endolysin PlyPy

2015-05-23T14:50:05Z

Bacterial viruses (bacteriophages) escape and kill their host by degrading the bacterial peptidoglycan layer through the mechanism of enzymes called endolysins: peptidoglycan degrading enzymes. The method included here is useful for the initial characterization of any endolysin, regardless of the specific catalytic domain (as long as the activity results in a reduction in the optical density), in ...

Rolf Lood, Vincent A. Fischetti (2014) Bio-protocol 4: 18 e1233

Bacteriophages infecting Propionibacterium acnes

2013-05-07T11:22:50Z

Viruses specifically infecting bacteria, or bacteriophages, are the most common biological entity in the biosphere. As such, they greatly influence bacteria, both in terms of enhancing their virulence and in terms of killing them. Since the first identification of bacteriophages in the beginning of the 20th century, researchers have been fascinated by these microorganisms and their ability to erad...

Holger Brüggemann, Rolf Lood (2013) BioMed Research International 2013: 705741

IgG glycan hydrolysis by EndoS diminishes the pro-inflammatory properties of immune complexes from patients with SLE - a possible new treatment?

2012-03-27T17:29:11Z

Lood C, Allhorn M, R Lood, Gullstrand B, Olin AI, Rönnblom L, Truedsson L, Collin M, Bengtsson AA (2012) Arthritis & Rheumatism :

Characterization and genome sequencing of two Propionibacterium acnes phages displaying pseudolysogeny

2011-09-19T11:08:43Z

Propionibacterium acnes is a Gram positive rod inhabiting the human skin that also infects orthopaedic implants and is associated with acne vulgaris. Previously, one lytic bacteriophage, PA6, from P. acnes has been sequenced and partially characterized. We recently isolated several inducible phages from P. acnes classified as Siphoviruses based on morphology and partial genome...

Rolf Lood and Mattias Collin (2011) BMC Genomics 12: 198

Biofilm formation by Propionibacterium acnes is a characteristic of invasive isolates

2011-09-19T11:12:02Z

Propionibacterium acnes is a common and probably underestimated cause of delayed joint prosthesis infection. Bacterial biofilm formation is central in the pathogenesis of infections related to foreign material, and P. acnes has been shown to form biofilm both in vitro and in vivo. Here, biofilm formation by 93 P. acnes isolates, either from invasive infections (n...

A Holmberg, R Lood, M Mörgelin, B Söderquist, E Holst, M Collin, B Christensson, M Rasmussen (2009) Clin Microbiol Infect : Published online

SufA - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation

2011-09-19T11:10:11Z

Finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. SufA was the first F. magna proteinase to be isolated and characterized. Many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified SufA efficiently...

C Karlsson, M Mörgelin, M Collin, R Lood, M Andersson, A Schmidtchen, L Björck, I M Frick (2009) Microbiology 155: 238-248

Inducible Siphoviruses in superficial and deep tissue isolates of Propionibacterium acnes

2011-09-19T11:16:11Z

Propionibacterium acnes is a commensal of human skin but is also known to be involved in certain diseases, such as acne vulgaris and infections of orthopaedic implants. Treatment of these conditions is complicated by increased resistance to antibiotics and/or biofilm formation of P. acnes bacteria. P. acnes can be infected by bacteriophages, but until recently little has been ...

Rolf Lood, Matthias Mörgelin, Anna Holmberg, Magnus Rasmussen, Mattias Collin (2008) BMC Microbiology 8: 139