Samantha Chan's Publications List

Evaluation of Three Catabolic Enzymes to induce Intervertebral Disc Degeneration in a bovine Organ Culture Model

2013-01-11T13:55:28Z

Introduction: Intervertebral disc degeneration (IDD) shows a balance shift from anabolic towards catabolic enzymes. These consist mainly of matrix metalloproteinases (MMPs), members of the ADAMTS (A Disintegrin And Metalloprotease with ThromboSpondin motifs)-family and HTRA1 (high temperature requirement serine protease A1), a serine protease recently recognized as a detrimental factor in IDD path...

T. Furtwängler, S. C. W. Chan, G. Bahrenberg , P. J. Richards , B. Gantenbein-Ritter (2013) Global Spine Congress 4-6 April 2013

Mechanobiological Study of the Intervertebral Disc under Complex Loading using a Bi-axial Loading Bioreactor

2013-01-11T13:58:18Z

Introduction: The intervertebral disc (IVD) functions to transmit the loading of the spine: the loadings applied to the disc are a complex combination of compression, torsion and bending. Complex loading strongly affects the disc's mechanical response and its ability to resist rupture. [1] Research on in vitro IVD mechanobiology has been limited to axial compression, whereas the importance of tors...

SCW Chan, J. Walser , P. Käppeli , M. J. Shamsollahi, S. J. Ferguson, B. Gantenbein-Ritter (2013) Global Spine Congress 4-6 April 2013, Hong Kong

A papain-induced disc degeneration model for the assessment of thermo-reversible hydrogel-cells therapeutic approach.

2013-01-13T10:37:04Z

Nucleus pulposus (NP) regeneration by the application of injectable cell-embedded hydrogels is an appealing approach for tissue engineering. We investigated a thermo-reversible hydrogel (TR-HG), based on a modified polysaccharide with a thermo-reversible polyamide [poly(N-isopropylacrylamide), pNIPAM], which is made to behave as a liquid at room temperature and hardens at > 32 °C. In order ...

C Malonzo, S C W Chan, A Kabiri, D Eglin, S Grad, H M Bonél, L M Benneker, B Gantenbein-Ritter (2013) J Tissue Eng Regen Med :

Assessment of the Matrix Degenerative Effects of MMP-3, ADAMTS-4 and HTRA1 injected into a bovine Intervertebral Disc Organ Culture Model.

2013-07-16T13:26:15Z

Study Design. In vitro study to develop an intervertebral disc degeneration (IDD) organ culture model, using coccygeal bovine intervertebral discs (IVDs) and injection of proteolytic enzymes MMP-3, ADAMTS-4 and HTRA1.Objective. This study aimed to develop an in-vitro model of enzyme-mediated IDD to mimic the clinical outcome in humans for investigation of therapeutic treatment options.Summary of B...

Tina Furtwängler, Samantha C W Chan, Gregor Bahrenberg, Peter J Richards, Benjamin Gantenbein-Ritter (2013) Spine :

Papain-induced in-vitro disc degeneration model for the study of injectable nucleus pulposus therapy

2013-01-11T14:01:18Z

BACKGROUND CONTEXT: Proteolytic enzyme digestion of the intervertebral disc (IVD) offers a method to simulate a condition of disc degeneration for the study of cell-scaffold constructs in the degenerated disc. PURPOSE: To characterize an in-vitro disc degeneration model (DDM) of different severity of glycosaminoglycans (GAG) and water loss by using papain and to determine the initial response of...

Chan SCW, Bürki A, Bonél H, Benneker L, Gantenbein-Ritter B (2013) The Spine J In press:

Region specific response of intervertebral disc cells to complex dynamic loading: an organ culture study using a dynamic torsion-compression bioreactor.

2014-02-05T20:36:52Z

The spine is routinely subjected to repetitive complex loading consisting of axial compression, torsion, flexion and extension. Mechanical loading is one of the important causes of spinal diseases, including disc herniation and disc degeneration. It is known that static and dynamic compression can lead to progressive disc degeneration, but little is known about the mechanobiology of the disc subje...

Samantha C W Chan, Jochen Walser, Patrick Käppeli, Mohammad Javad Shamsollahi, Stephen J Ferguson, Benjamin Gantenbein-Ritter (2013) PloS one 8: 8

Evolution of In-vitro Organ Culture Models for the Intervertebral Disc

2013-01-11T11:03:09Z

Back pain is a common health problem in modern western society, where back pain can be caused by disc degeneration. An in-vitro disc organ culture model could help answer questions concerning causes of disc degeneration and for testing various disc regeneration strategies. The overall aim of this thesis is to develop a new genera- tion disc organ culture system with complex loading, consisting of ...

Samantha CW Chan (2012)

Optimization of the Enzyme-induced Disc Degeneration Model for the in-vitro study of Nucleus Pulposus Tissue Engineering.

2011-11-18T10:44:59Z

Gantenbein-Ritter B, Benneker L, Siebenrock K, Chan SC. (2012) Proceedings of the 58th Annual Meeting of the Orthopaedic Research Society

Discogenic Pre-Conditioning of human Mesenchymal Stem Cells in synthetic MMP-Degradable Poly-Ethylene-Glycol (PEG) Hydrogels under Load and rhGDF-5

2011-11-18T10:51:20Z

B Gantenbein, S Guggisberg, S C Chan, L Benneker, S Grad (2012) Proceedings of the 58th Annual Meeting of the Orthopaedic Research Society, San Francisco

Ein reproduzierbares Papain-induziertes Bandscheibendegenerations-Modell mit Reduziertem GAG und Wassergehalt

2013-01-11T11:10:20Z

1. Einleitung Nucleus pulposus (NP) Verdau mittels Chymopapain ist ein klinisches Verfahren um die Bandscheibenherniation zu behandeln. Der Verdau führt zu einem reduzierten Wasser- und Glykosaminoglykan (GAG) Gehalt. Das Ziel dieser Studie ist es ein in vitro Bandscheiben- Degenerationsmodell (DDM) zu testen, wo verschiedene Dosen von Papain in bovine Schwanzbandscheiben injiziert werden, die d...

SCW Chan, L Benneker, H Bonel, B Gantenbein (2012) 7. Deutscher Wirbelsäulenkongress, 6- 08 Dezember 2012, in Stuttgart, Deutschland.

Preparation of intact bovine tail intervertebral discs for organ culture.

2013-01-11T14:02:39Z

The intervertebral disc (IVD) is the joint of the spine connecting vertebra to vertebra. It functions to transmit loading of the spine and give flexibility to the spine. It composes of three compartments: the innermost nucleus pulposus (NP) encompassing by the annulus fibrosus (AF), and two cartilaginous endplates connecting the NP and AF to the vertebral body on both sides. Discogenic pain possib...

S C W Chan, B Gantenbein-Ritter (2012) J Vis Exp : 60

Intervertebral disc regeneration or repair with biomaterials and stem cell therapy - feasible or fiction?

2012-06-03T12:04:00Z

The "gold standard" for treatment of intervertebral disc herniations and degenerated discs is still spinal fusion, corresponding to the saying "no disc - no pain". Mechanical prostheses, which are currently implanted, do only have medium outcome success and have relatively high re-operation rates. Here, we discuss some of the biological intervertebral disc replacement approaches, which can be subd...

SCW Chan, B Gantenbein-Ritter (2012) Swiss Med Wkly 142:

New generation of bi-axial mechanical loading bioreactor for the intervertebral disc

2013-01-11T11:13:46Z

SCW Chan, J Walser, P Käpperli, MJ Shamsollahi, SJ Ferguson, B Gantenbein (2012) Annual meeting of The Swiss Society for Biomedical Engineering.

A Reproducible Papain-induced in-vitro Disc Degeneration Model for the Study of Injectable Nucleus Pulposus therapy

2013-01-13T10:32:58Z

S. C. W. Chan, L. M. Benneker, K. Siebenrock, B. Gantenbein-Ritter (2012) SpineWeek 2012 Amsterdam, The Netherlands on May 28 – June 1

A Reproducible Papain-induced Disc Degeneration Model of reduced GAG and Hydration

2013-01-13T10:28:15Z

Introduction: Nucleus pulposus (NP) digestion using chymopapain is a clinical procedure to release pressure of a herniated disc. It reduces matrix content and hence offers a method to simulate a condition of disc degeneration with reduced glycosaminoglycans (GAG) and water content. [1] The aims of the study is first to characterize a set of in-vitro disc degeneration model (DDM) of different sever...

S. C. W. Chan, L. M. Benneker, K. Siebenrock, B. Gantenbein-Ritter (2012) World Forum for Spine Research 2012 in Helsinki, Finland June 18 - 21

Thermo-Reversible Hydrogel- for Nucleus Pulposus Replacement: Feasibility under Static Loading in a mild Papain-induced Disc Degeneration Model

2013-01-13T10:25:53Z

Introduction: Nucleus pulposus (NP) replacement by the application of injectable hydrogels seems a straightforward approach for tissue engineering. We investigate a thermo-reversible hydrogel (TH-RHG), based on a modified polysaccharide with a thermo-reversible polyamide (poly(N-isopropylacrylamide = pNIPAM). Using “click chemistry” and reversible addition fragmentation transfer (RAFT) polymer...

C. Malonzo, S. C. W. Chan, H. M. Bonél, L. M. Benneker, B. Gantenbein-Ritter (2012) World Forum for Spine Research 2012, Helsinki 18-21 June

Mesenchymal stem cell therapy in an enzymatic-induced disc degeneration model: a feasibility study.

2011-11-18T10:48:32Z

Chan SC, Benneker L, Siebenrock K, Gantenbein-Ritter B. (2011) Annual meeting of The Swiss Society for Biomedical Engineering

The Evolutionary Importance of Cell Ratio between Notochordal and Nucleus Pulposus Cells - An Experimental 3D Co-culture Study

2011-03-17T13:39:09Z

Gantenbein-Ritter B, Chan SCW (2011) Proceedings of the 57th Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, 13-16 January, 2011

Repetitive Torsion Activates Matrix Remodeling of the Intervertebral Disc

2011-03-17T13:39:09Z

Chan SCW, Ferguson SJ, Wuertz K, Gantenbein-Ritter B. (2011) Proceedings of the 57th Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, 13-16 January, 2011

The evolutionary importance of cell ratio between notochordal and nucleus pulposus cells: an experimental 3-D co-culture study.

2012-02-03T15:04:12Z

INTRODUCTION: Notochordal cells and nucleus pulposus cells are co-existing in the intervertebral disc at various ratios among different mammalians. This fact rises the question about the interactions and the evolutionary relevance of this phenomenon. It has been described that these relatively large notochordal cells are mainly dominant in early lifetime of all vertebrates an...

Benjamin Gantenbein-Ritter, Samantha C W Chan (2011) Eur Spine J :

Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers

2011-03-17T13:39:09Z

B Gantenbein-Ritter, C M Sprecher, S W C Chan, S Illien-Jünger, S Grad (2011) Mammalian Cell Viability Methods

Nucleus Pulposus Tissue Engineering using Poly-Ethylene-Glycol Hydrogel and Mesenchymal Stem Cells in an ex-vivo Disc Degeneration Model

2011-11-18T10:46:54Z

S.C.W. Chan, L. Benneker, K.A. Siebenrock, B. Gantenbein-Ritter (2011) The symposium of AO Exploratory Research: Where Science meets Clinics

Interaction of Synthetic PEG Scaffold, Growth Factors and Mechanical Loading on Mesenchymal Stem Cells

2011-11-18T10:47:08Z

S. Guggisberg, S C W Chan, LM Benneker and B. Gantenbein-Ritter (2011) The symposium of AO Exploratory Research: Where Science meets Clinics

The role of cryopreservation in the biomechanical properties of the intervertebral disc.

2012-04-08T13:39:17Z

Implantation of intervertebral disc (IVD) allograft or tissue engineered disc constructs in the spine has emerged as an alternative to artificial disc replacement for the treatment of severe degenerative disc disease (DDD). Establishment of a bank of cryopreserved IVD allografts enables size matching and facilitates logistics for effective clinical management. However, the biomechanical properties...

S K L Lam, S C W Chan, V Y L Leung, W W Lu, K M C Cheung, K D K Luk (2011) Eur Cell Mater 22: 393-402

Biological response of the intervertebral disc to repetitive short-term cyclic torsion.

2012-02-03T15:04:12Z

In vitro study of the biological response of the intervertebral disc (IVD) to cyclic torsion by using bovine caudal IVDs.

Samantha C W Chan, Stephen J Ferguson, Karin Wuertz, Benjamin Gantenbein-Ritter (2011) Spine (Phila Pa 1976) 36: 24 2021-2030

The effects of dynamic loading on the intervertebral disc.

2012-02-03T15:04:12Z

Loading is important to maintain the balance of matrix turnover in the intervertebral disc (IVD). Daily cyclic diurnal assists in the transport of large soluble factors across the IVD and its surrounding circulation and applies direct and indirect stimulus to disc cells. Acute mechanical injury and accumulated overloading, however, could induce disc degeneration. Recently, there is more informatio...

Samantha C W Chan, Stephen J Ferguson, Benjamin Gantenbein-Ritter (2011) Eur Spine J 20: 11 1796-1812

Effect of Short-Term Torsion to the Intervertebral Disc

2011-03-17T13:39:09Z

Gantenbein-Ritter B., Chan, S. and Ferguson, S.J. (2010) World Forum of Spine Research - The Intervertebral Disc. Montréal, Canada, 2010

Intervertebral Disc and Torsion.

2011-06-01T14:19:28Z

Chan SCW, Ferguson SJ, Wuertz K, Gantenbein-Ritter B. (2010) Swiss-Japanese Minisymposium on Disc/Spine Research. Irchel University, Zürich (September 6, 2010)

Effect of Short Term Torsion to the Intervertebral Disc: An Organ Culture Study

2011-03-17T13:39:09Z

Chan, S.C.W., Ferguson, S.J., Wuertz, K. and Gantenbein-Ritter, B. (2010) Proceedings of the eCM XI Congress, Davos, Switzerland, 2010

Quantifying Cell Shape Changes of Human Mesenchymal Stem Cells undergoing Differentiation

2011-03-17T13:39:09Z

Gantenbein-Ritter B, Seiler C, Chan SCW, Kühn J, Depeursinge C. (2010) Proceedings of ECM XI, Davos, 2010

Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs.

2012-02-03T15:04:12Z

Severe intervertebral disc (IVD) degeneration often requires disc excision and spinal fusion, which leads to loss of spinal segment mobility. Implantation of an allograft disc or tissue engineered disc construct emerges as an alternative to artificial disc replacement for preserving the motion of the degenerated level. Establishment of a bank of cadaveric or engineered cryopreserved discs enables ...

Samantha C W Chan, Stephen K L Lam, Victor Y L Leung, Danny Chan, Keith D K Luk, Kenneth M C Cheung (2010) Eur Cell Mater 19: 273-283

Cryopreserved intervertebral disc with injected bone marrow-derived stromal cells: a feasibility study using organ culture.

2012-02-03T15:04:12Z

A recent clinical study demonstrated that cryopreserved allogeneic intervertebral disc transplantation relieved pain and preserved motion, thus opening up a new treatment option for degenerative disc disease. However, these transplanted discs continued to degenerate, possibly due to a lack of viable cells. Bone marrow-derived stromal cell (BMSC) implantation has been shown to delay disc degenerati...

Samantha C W Chan, Benjamin Gantenbein-Ritter, Victor Y L Leung, Danny Chan, Kenneth M C Cheung, Keita Ito (2010) Spine J 10: 6 486-496

Torsion Increases Intervertebral Disc Cell Survival in Organ Culture

2011-03-17T13:39:09Z

Chan, S.C.W., Ferguson, S.J., Gantenbein-Ritter B. (2010) Proceedings of the 17th Congress of the European Society of Biomechanics, Edinburgh, United Kingdom, 2010

Effect of Cryopreservation on the Biomechanical Properties of the Intervertebral Discs

2012-04-08T13:48:09Z

Intervertebral Disc (IVD) allograft transplantation is presented to be a practical treatment of severe Degenerative Disc Disease (DDD). Limited availability of fresh allograft tissues necessitate the establishment of tissue banking for long-term storage. However, the effects of the cryopreservants on the mechanical properties of the IVD remains unknown. We hypothesize that the appropriate use of c...

S.K.L. Lam, S. C. W. Chan, V. Y. L. Leung, W. W. Lu, K. M. C. Cheung and K. D. K. Luk (2009) 13TH INTERNATIONAL CONFERENCE ON BIOMEDICAL ENGINEERING 1252-1254

Intervertebral disc cryopreservation.

2011-06-03T13:19:58Z

Chan S, Leung V, Chan D, Lu W, Luk K, Cheung K. (2008) Proceeding of the 29th SICOT(Société Internationale de Chirurgie Orthopédique et de Traumatologie), 24-28 Aug 2008,Hong Kong SAR

Fate of Bone Marrow-Derived Stromal Cells injected into Cell-depleted Intervertebral Disc in Physiological Organ Culture.

2011-06-03T13:15:14Z

Gantenbein B, Chan S, , Chan D, Lu W, Cheung K, Ito K (2008) Proceedings of the 54th Annual Meeting of the Orthopaedic Research Society, San Francisco

In vitro study of the cryopreserved intervertebral disc

2011-06-01T14:15:56Z

Degenerative disc disease (DDD) is one of the major causes of low back pain. Conventional surgical treatments for DDD can alleviate pain but usually results in fusion of the motion segment and consequently leads to adjacent level disc degeneration. Clinical result of transplanting cryopreserved allograft disc for DDD treatment has demonstrated satisfactory result in motion and stability preservati...

Samantha CW Chan (2008)

Cell Repopulation of the frozen intervertebral disc by bone marrow-derived stem cells.

2011-06-01T14:08:48Z

Chan S, Gantenbein B, Leung V., Chan D, Lu W, Cheung K, Ito K. (2008) AO Spine Forum, 23-27 June 2008, Kyoto, Japan.

Fate of mesenchymal stem cells injected into intervertebral discs

2011-06-01T14:22:46Z

Chan S, Gantenbein B, Chan D, Lu W, Cheung K, Ito K (2007) Proceedings of the 34th Annual Meeting of the International Society for the Study of the Lumbar Spine 10-14 June, Hong Kong.

Surface mechanical properties, corrosion resistance, and cytocompatibility of nitrogen plasma-implanted nickel-titanium alloys: a comparative study with commonly used medical grade materials.

2012-04-08T13:40:42Z

Stainless steel and titanium alloys are the most common metallic orthopedic materials. Recently, nickel-titanium (NiTi) shape memory alloys have attracted much attention due to their shape memory effect and super-elasticity. However, this alloy consists of equal amounts of nickel and titanium, and nickel is a well known sensitizer to cause allergy or other deleterious effects in living tissues. Ni...

K W K Yeung, R W Y Poon, P K Chu, C Y Chung, X Y Liu, W W Lu, D Chan, S C W Chan, K D K Luk, K M C Cheung (2007) J Biomed Mater Res A 82: 2 403-414

Outcome assessment of bracing in adolescent idiopathic scoliosis by the use of the SRS-22 questionnaire.

2012-04-08T13:41:15Z

The SRS-22 questionnaire is specifically designed for the assessment of quality of life in spinal deformity patients. This study is the first to use it to assess the quality of life of adolescent idiopathic scoliosis patients under brace treatment and compares the results with an observational group matched by age and curve magnitude. Forty-six patients were enrolled into each group. Overall, it w...

Kenneth M C Cheung, Elaine Y L Cheng, Samantha C W Chan, Kelvin W K Yeung, Keith D K Luk (2007) Int Orthop 31: 4 507-511

Suppression of nickel release in nickel-titanium alloys by plasma immersion ion implantation surface treatment: towards a new generation of "smart" orthopaedic implants

2011-06-03T13:13:31Z

KWK Yeung, YL Chan, SCW Chan, XY Liu, CY Chung, PK Chu, WW Lu, KDK Luk, D Chan, KMC Cheung. (2006) Proceeding of 33rd IEEE International Conference on Plasma Science. Traverse City, Michigan, USA. Jun 4-8

Prevention of oxidative degradation of polyurethane by covalent attachment of di-tert-butylphenol residues.

2012-04-08T13:41:45Z

Polyurethane (PU) components of cardiovascular devices are subjected to oxidation-initiated surface degradation, which leads to cracking and ultimately device failure. In the present study, we investigated a novel bromoalkylation chemical strategy to covalently attach the antioxidant, di-tert-butylphenol (DBP), and/or cholesterol (Chol) to the PU urethane nitrogen groups to hypothetically prevent ...

Stanley J Stachelek, Ivan Alferiev, Hoon Choi, Chun Wai Chan, Brett Zubiate, Michael Sacks, Russell Composto, I-Wei Chen, Robert J Levy (2006) J Biomed Mater Res A 78: 4 653-661

Quantitative studies of the interaction between plasma and tissue components in human intima.

2014-02-05T20:36:04Z

E B Smith, K M Alexander, I B Massie (1977) Advances in experimental medicine and biology 82: 872-877

Microcalorimetric study of the anaerobic growth of Escherichia coli: growth thermograms in a synthetic medium.

2014-02-05T20:36:04Z

A microcalorimetric technique was used for studying the growth of Escherichia coli during anaerobiosis. The growth thermograms obtained are complex and the shape of curves is dependent on the hydrogen lyase activity of the cells. Fermentation balances are given for different culture conditions, and simple growth thermograms are obtained when the hydrogen lyase activity is inhibitied.

A Belaich, J P Belaich (1976) Journal of bacteriology 125: 1 14-18