Seiji Okada's Publications List

Identification of a population of cells with hematopoietic stem cell properties in mouse aorta-gonad-mesonephros cultures

2010-05-25T05:48:56Z

The aorta-gonad-mesonephros (AGM) region is a primary source of definitive hematopoietic cells in the midgestation mouse embryo. In cultures of dispersed AGM regions, adherent cells containing endothelial cells are observed first, and then non-adherent hematopoietic cells are produced. Here we report on the characterization of hematopoietic cells that emerge in the AGM culture. Based on the expres...

I Nobuhisa, N Ohtsu, S Okada, N Nakagata, T Taga (2007) Exp Cell Res http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17275811 313: 5 965-74

Heat shock suppresses human NK cell cytotoxicity via regulation of perforin

2010-05-25T05:48:56Z

Human natural killer (NK) cell, which is an important lymphocyte for immune surveillance, is highly sensitive to heat, but the nature of its response to and its mechanistic regulation by heat remain unclear. Here we determined the effect of in vitro heat shock and in vivo hyperthermia on human NK cell cytotoxicity. Human peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers we...

H Harada, T Murakami, S S Tea, A Takeuchi, T Koga, S Okada, M A Suico, T Shuto, H Kai (2007) Int J Hyperthermia http://www.informaworld.com/smpp/content~db=all?content=10.1080/02656730701822087 23: 8 657-65

Proliferative activation up-regulates expression of CD4 and HIV-1 co-receptors on NK cells and induces their infection with HIV-1

2010-05-25T05:48:56Z

NK cells play important roles in immune surveillance against malignancy and virus-infected cells. NK cell functions are affected in patients infected with HIV-1; however, whether there is direct interaction between NK cells and HIV-1 remains controversial. In this study the expression of CD4, an important receptor for HIV-1, was up-regulated on NK cells co-cultured with an NK cell-selective stimul...

H Harada, Y Goto, T Ohno, S Suzu, S Okada (2007) Eur J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17628860 37: 8 2148-55

Granulocyte colony-stimulating factor attenuates neuronal death and promotes functional recovery after spinal cord injury in mice

2010-05-25T05:48:56Z

Granulocyte colony-stimulating factor (G-CSF) is a protein that stimulates differentiation, proliferation, and survival of granulocytic lineage cells. Recently, a neuroprotective effect of G-CSF was reported in a model of cerebral infarction. The aim of the present study was to elucidate the potential therapeutic effect of G-CSF for spinal cord injury (SCI) in mice. We found that G-CSF is neuropro...

Y Nishio, M Koda, T Kamada, Y Someya, R Kadota, C Mannoji, T Miyashita, S Okada, A Okawa, H Moriya, M Yamazaki (2007) J Neuropathol Exp Neurol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17882016 66: 8 724-31

Granulocyte colony-stimulating factor (G-CSF) mobilizes bone marrow-derived cells into injured spinal cord and promotes functional recovery after compression-induced spinal cord injury in mice

2010-05-25T05:48:56Z

The aim of the present study was to elucidate the effects of granulocyte colony-stimulating factor (G-CSF)-mediated mobilization of bone marrow-derived stem cells on the injured spinal cord. Bone marrow cells of green fluorescent protein (GFP) transgenic mice were transplanted into lethally irradiated C57BL/6 mice. Four weeks after bone marrow transplantation, spinal cord injury was produced by a ...

M Koda, Y Nishio, T Kamada, Y Someya, A Okawa, C Mori, K Yoshinaga, S Okada, H Moriya, M Yamazaki (2007) Brain Res http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17391650 1149: 223-31

The use of hemopoietic stem cells derived from human umbilical cord blood to promote restoration of spinal cord tissue and recovery of hindlimb function in adult rats

2010-05-25T05:48:56Z

OBJECT: The use of human umbilical cord blood (HUCB) cells has been reported to improve functional recovery in cases of central nervous system injuries such as stroke, traumatic brain injury, and spinal cord injury (SCI). The authors investigated the effects of hemopoietic stem cells that were derived from HUCB and transplanted into the injured spinal cords of rats. METHODS: One week after injury,...

Y Nishio, M Koda, T Kamada, Y Someya, K Yoshinaga, S Okada, H Harada, A Okawa, H Moriya, M Yamazaki (2006) J Neurosurg Spine http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17120892 5: 5 424-33

Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis

2010-05-25T05:48:56Z

Suppressors of cytokine signaling (SOCS) proteins are negative regulators of cytokine signaling by inhibiting the JAK-STAT signal transduction pathway, but their role in innate immunity remains to be investigated. In the present study, we demonstrate that overexpression of SOCS5 in T cells augments innate immunity during septic peritonitis induced by cecal ligation and puncture (CLP). Mice with a ...

H Watanabe, M Kubo, K Numata, K Takagi, H Mizuta, S Okada, T Ito, A Matsukawa (2006) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17142765 177: 12 8650-7

BT-IgSF, a novel immunoglobulin superfamily protein, functions as a cell adhesion molecule

2010-05-25T05:48:56Z

BT-IgSF is a newly identified cell surface glycoprotein belonging to the immunoglobulin superfamily (IgSF). We have previously shown that the expression of the BT-IgSF gene was highly restricted to brain and testis, and its transcript was detected in both neurons and glial cells. In this study, to explore its function, we generated cells overexpressing BT-IgSF proteins and analyzed their phenotype...

H Harada, S Suzu, Y Hayashi, S Okada (2005) J Cell Physiol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15795899 204: 3 919-26

Selective expansion and engraftment of human CD16+ NK cells in NOD/SCID mice

2010-05-25T05:48:56Z

NK cells are large granular lymphocytes that represent a critical component of the innate immunity. Investigations of human NK cell function are largely based on in vitro assays because of the lack of suitable animal models. Here we have established conditions leading to the development of human NK cells in NOD/SCID (severe combined immunodeficiency) mice receiving grafts of cord blood mononuclear...

H Harada, S Suzu, T Ito, S Okada (2005) Eur J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16304638 35: 12 3599-609

Abnormal erythroid differentiation in neonatal bcl-6-deficient mice

2010-05-25T05:48:56Z

OBJECTIVE: The bcl-6 proto-oncogene is ubiquitously expressed in various tissues. Since we found out the smaller number of TER119(+) cells in the spleen of neonatal bcl-6-deficient (bcl-6(-/-)) mice compared with that of control (bcl-6(+/+)) littermates, we studied functions of bcl-6 in differentiation of erythroid lineage cells. MATERIALS AND METHODS: Erythroblasts in the definitive erythropoiesi...

S Asari, A Sakamoto, S Okada, Y Ohkubo, M Arima, M Hatano, Y Kuroda, T Tokuhisa (2005) Exp Hematol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15661395 33: 1 26-34

HIV-1 Nef interferes with M-CSF receptor signaling through Hck activation and inhibits M-CSF bioactivities

2010-05-25T05:48:56Z

HIV-1 Nef protein is a major determinant of the pathogenicity of the virus. It has been shown that Nef activates Hck, a member of Src family kinase, in monocytes/macrophages and that the interaction is critical for AIDS-like disease progression in a mouse model. However, it was unclear how the molecular interaction in monocytes/macrophages leads to disease progression. Here, we show for the first ...

S Suzu, H Harada, T Matsumoto, S Okada (2005) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15626739 105: 8 3230-7

Hematopoietic stem cell and marrow stromal cell for spinal cord injury in mice

2010-05-25T05:48:56Z

We compared the effects of hematopoietic stem cell and marrow stromal cell transplantation for spinal cord injury in mice. From green fluorescent protein transgenic mouse bone marrow, lineage-negative, c-kit- and Sca-1-positive cells were sorted as hematopoietic stem cells and plastic-adherent cells were cultured as marrow stromal cells. One week after injury, hematopoietic stem cells or marrow st...

M Koda, S Okada, T Nakayama, S Koshizuka, T Kamada, Y Nishio, Y Someya, K Yoshinaga, A Okawa, H Moriya, M Yamazaki (2005) Neuroreport http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16237323 16: 16 1763-7

A role for c-fos/activator protein 1 in B lymphocyte terminal differentiation

2010-05-25T05:48:56Z

Expression of B lymphocyte-induced maturation protein 1 (Blimp-1) transcription factor is essential for promoting B cell differentiation into plasma cells. However, a critical transcription factor for Blimp-1 expression in activated B cells is unclear. When splenic B cells were stimulated with CD40 ligand (CD40L) and IL-4, terminal differentiation was induced in the B cells from c-fos transgenic (...

Y Ohkubo, M Arima, E Arguni, S Okada, K Yamashita, S Asari, S Obata, A Sakamoto, M Hatano, O Wang J, M Ebara, H Saisho, T Tokuhisa (2005) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15944271 174: 12 7703-10

Hyperthermia suppresses the cytotoxicity of NK cells via down-regulation of perforin/granzyme B expression

2010-05-25T05:48:56Z

Hyperthermia, which is used as an adjunctive therapy for cancer, is known to modulate the activity of natural killer (NK) cells in vitro, but its effect in vivo is unclear. In the present study, we used a whole body hyperthermia (WBH) device heated by infrared rays to evaluate the effect of WBH on mice models. We demonstrate here that wild type C57BL/6J mice exposed to 42 degrees C for 60min had r...

T Koga, H Harada, T S Shi, S Okada, M A Suico, T Shuto, H Kai (2005) Biochem Biophys Res Commun http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16236268 337: 4 1319-23

Beta-galactosidase of ROSA26 mice is a useful marker for detecting the definitive erythropoiesis after stem cell transplantation

2010-05-25T05:48:56Z

BACKGROUND: Hematopoietic reconstitution after stem cell transplantation has been analyzed by using stem cells of Ly5 congenic mice. However, the early erythropoiesis has never been analyzed because this marker is not expressed on all of the erythroid lineage cells. The transgenic mouse expressing beta-galactosidase (beta-gal) or green fluorescent protein (GFP) has been reported. Using these marke...

S Asari, S Okada, Y Ohkubo, A Sakamoto, M Arima, M Hatano, Y Kuroda, T Tokuhisa (2004) Transplantation http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15446309 78: 4 516-23

Bcl6 acts as an amplifier for the generation and proliferative capacity of central memory CD8+ T cells

2010-05-25T05:48:56Z

Central memory CD8(+) T cells (T(CM)) are considered to be more efficient than effector ones (T(EM)) for mediating protective immunity. The molecular mechanism involved in the generation of these cells remains elusive. Because Bcl6 plays a role in the generation and maintenance of memory CD8(+) T cells, we further examined this role in the process in relation to T(CM) and T(EM) subsets. In this st...

H Ichii, A Sakamoto, Y Kuroda, T Tokuhisa (2004) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15240675 173: 2 883-91

Impaired lymphocyte development and function in Clast5/Stra13/DEC1-transgenic mice

2010-05-25T05:48:56Z

Clast5/Stra13/DEC1 is a member of the helix-loop-helix family of transcriptional repressors. We have previously shown that Clast5 is rapidly down-regulated upon B cell activation and its overexpression inhibits cell cycle progression in B lymphoma cells. In the present study, we show that Clast5 expression is developmentally regulated during B cell differentiation, being expressed at the progenito...

M Seimiya, A Wada, K Kawamura, A Sakamoto, Y Ohkubo, S Okada, M Hatano, T Tokuhisa, T Watanabe, H Saisho, M Tagawa, O Wang J (2004) Eur J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15114665 34: 5 1322-32

DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers

2010-05-25T05:48:56Z

DNA polymerase theta (Pol theta) is a recently identified family A polymerase that contains an intrinsic helicase domain. Drosophila Pol theta mutants are hypersensitive to bifunctional DNA crosslinking agents and exhibit an elevated frequency of spontaneous chromosomal aberrations, suggesting a role for Pol theta in repair of DNA interstrand crosslinks and in the general maintenance of genome sta...

K Kawamura, R Bahar, M Seimiya, M Chiyo, A Wada, S Okada, M Hatano, T Tokuhisa, H Kimura, S Watanabe, I Honda, S Sakiyama, M Tagawa, O Wang J (2004) Int J Cancer http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14735462 109: 1 9-16

Transplanted hematopoietic stem cells from bone marrow differentiate into neural lineage cells and promote functional recovery after spinal cord injury in mice

2010-05-25T05:48:56Z

Recovery in central nervous system disorders is hindered by the limited ability of the vertebrate central nervous system to regenerate lost cells, replace damaged myelin, and re-establish functional neural connections. Cell transplantation to repair central nervous system disorders is an active area of research, with the goal of reducing functional deficits. Recent animal studies showed that cells...

S Koshizuka, S Okada, A Okawa, M Koda, M Murasawa, M Hashimoto, T Kamada, K Yoshinaga, M Murakami, H Moriya, M Yamazaki (2004) J Neuropathol Exp Neurol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14748562 63: 1 64-72

Adenovirus-mediated gene transfer of Bcl-xL impedes neurite regeneration in vitro

2010-05-25T05:48:56Z

Mouse retinal explants were transfected with recombinant adenovirus vector carrying the green fluorescent protein (GFP) gene and the rat bcl-x(L) gene (Adeno-Bcl-xL) to determine its ability to protect retinal ganglion cells against apoptotic cell death and to promote retinal ganglion cell neurite regeneration. Adeno-Bcl-xL-incubated retinas had reduced apoptosis compared with controls. However, n...

T Oshitari, S Okada, T Tokuhisa, E Adachi-Usami (2003) Neuroreport http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12821800 14: 8 1159-62

Overexpression of the c-fos gene perturbs functional maturation of M1 cells into macrophages

2010-05-25T05:48:56Z

Expression of the proto-oncogene c-fos is induced in normal myelopoiesis. However, functions of c-Fos in the process of differentiation towards macrophages are still controversial. To explore the functions, we used the murine myeloblastic leukemia cell line M1. Stimulation of M1 cells with bacterial LPS promotes their terminal differentiation into functional macrophages. Overexpression of c-fos in...

S Obata, S Okada, Y Ohkubo, M Murasawa, M Hatano, S Sekiya, T Tokuhisa (2003) Mol Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12431392 39: 10 585-94

Dominant-negative effect of the c-fos family gene products on inducible NO synthase expression in macrophages

2010-05-25T05:48:56Z

Activation of murine peritoneal macrophages or the macrophage cell line RAW264 with IFN-gamma and bacterial lipopolysaccharide promotes a transient up-regulation of c-fos family gene expression following inducible NO synthase (iNOS) production. Since introduction of a double mutation into the two AP-1-binding sites in the iNOS promoter region reduced the promoter activity to 25% of the authentic o...

S Okada, S Obata, M Hatano, T Tokuhisa (2003) Int Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14565925 15: 11 1275-82

Bcl6 is a transcriptional repressor for the IL-18 gene

2010-05-25T05:48:56Z

Bcl6 functions as a sequence-specific transcriptional repressor, and Bcl6-deficient (Bcl6(-/-)) mice have been reported to display Th2-type inflammatory diseases in multiple organs. Since IL-18 is a potent stimulator of Th2 cells, we examined the expression of IL-18 mRNA in bone marrow-derived macrophages from Bcl6(-/-) mice after LPS stimulation. Here we show that the expression was strikingly up...

N Takeda, M Arima, N Tsuruoka, S Okada, M Hatano, A Sakamoto, Y Kohno, T Tokuhisa (2003) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12817026 171: 1 426-31

Bcl6-dependent transcriptional repression by BAZF

2010-05-25T05:48:56Z

BAZF, a member of Bcl6 gene family, acts as a sequence-specific transcriptional repressor in various cells including NIH3T3 cells. The DNA-binding sequence for BAZF is the same as that for Bcl6 and the repressor activity of BAZF was also inhibited by Tricostatin A, an inhibitor of histone deacetylase, suggesting the functional homology between them. However, BAZF unlike Bcl6 cannot function as a t...

M Takenaga, M Hatano, M Takamori, Y Yamashita, S Okada, Y Kuroda, T Tokuhisa (2003) Biochem Biophys Res Commun http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12659862 303: 2 600-8

Downregulation of bcl-xL is relevant to UV-induced apoptosis in fibroblasts

2010-05-25T05:48:56Z

Exposure to ultraviolet light (UV) induces apoptosis in mammalian cells. The caspase group of proteases is required for the apoptosis. This pathway is initiated by a release of cytochrome c from the mitochondria into the cytosol. Several Bcl-2 family proteins can regulate the release of cytochrome c by stabilizing the mitochondrial membrane. Here we show that expression of the endogenous bcl-xL wa...

Y Nakagawa, S Okada, M Hatano, M Ebara, H Saisho, T Tokuhisa (2002) J Biochem Mol Biol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12359085 35: 5 452-8

GL7 defines the cycling stage of pre-B cells in murine bone marrow

2010-05-25T05:48:56Z

We have identified a novel subset of early B lineage cells in the mouse bone marrow (BM) by GL7 expression on cell surface. GL7(+)B220(low) BM cells have a large cell size and are CD43(-to low), CD95(-), Sca-1(-), I-A(low), IgM(-) and IgD(-), suggesting that they are large pre-B cells. These BM cells express lambda5 and VpreB but not terminal deoxytransferase (TdT) and Bcl-2, and approximately 50 ...

M Murasawa, S Okada, S Obata, M Hatano, H Moriya, T Tokuhisa (2002) Eur J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11782020 32: 1 291-8

Growth retardation, polyploidy, and multinucleation induced by Clast3, a novel cell cycle-regulated protein

2010-05-25T05:48:56Z

We have identified a novel gene, Clast3, by subtraction of cDNAs derived from activated and naive B lymphocytes. Clast3 expression is elevated in cycling cells and down-regulated in cells undergoing growth arrest, indicating that its expression is controlled in a cell cycle-dependent manner. The deduced amino acid sequence of Clast3 cDNA exhibits no significant homology to the known proteins in ma...

R Bahar, O Wang J, K Kawamura, M Seimiya, Y Wang, M Hatano, S Okada, T Tokuhisa, T Watanabe, M Tagawa (2002) J Biol Chem http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12147697 277: 42 40012-9

A putative silencer element in the IL-5 gene recognized by Bcl6

2010-05-25T05:48:56Z

The Bcl6 gene is ubiquitously expressed in adult murine tissues and its product functions as a sequence-specific transcriptional repressor. Bcl6-deficient mice displayed eosinophilic inflammation caused by overproduction of Th2 cytokines. The regulatory mechanism of those cytokine productions by Bcl6 is controversial. When CD4(+) T cells from Bcl6-deficient and lck-Bcl6-transgenic mice were stimul...

M Arima, H Toyama, H Ichii, S Kojima, S Okada, M Hatano, G Cheng, M Kubo, T Fukuda, T Tokuhisa (2002) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12097386 169: 2 829-36

Overexpression of TIAP/m-survivin in thymocytes enhances cell proliferation

2010-05-25T05:48:56Z

TIAP/m-survivin, a member of the inhibitor of apoptosis (IAP) protein family, is expressed in a cell cycle dependent manner. It is strongly expressed in various subsets of thymocytes. To investigate a role of TIAP/m-survivin in thymocytes, mice carrying the lck-TIAP transgene were established. Two out of six transgenic mice expressed large amounts of TIAP mRNA and protein in thymocytes. Although T...

S Hikita, M Hatano, A Inoue, N Sekita, K Kobayashi, M Otaki, T Ogasawara, S Okada, H Hirasawa, T Tokuhisa (2002) Mol Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12220887 39: 5-6 289-98

Role for Bcl-6 in the generation and maintenance of memory CD8+ T cells

2010-05-25T05:48:56Z

Naive T cells proliferate and differentiate into memory cells after antigenic stimulation or in a lymphopenic environment. We showed here transient increases in memory phenotype CD8+ T cell numbers in the lymphopenic environment of spleens of very young mice. The magnitude of the increase correlated with Bcl-6 expression in the T cells. Bcl-6 controlled the generation and maintenance of antigen-sp...

H Ichii, A Sakamoto, M Hatano, S Okada, H Toyama, S Taki, M Arima, Y Kuroda, T Tokuhisa (2002) Nat Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12021781 3: 6 558-63

The role of c-fos in cell death and regeneration of retinal ganglion cells

2010-05-25T05:48:56Z

PURPOSE: To investigate the effect of c-fos on apoptotic cell death and regeneration of damaged retinal ganglion cells (RGCs) in tissue culture of retinal explants. METHODS: Retinas from transgenic mice carrying the exogenous c-fos gene under the control of the interferon (IFN)-alpha/beta inducible Mx-promoter (Mx-c-fos), c-fos-deficient mice, and littermate control mice were dissected and culture...

T Oshitari, M Dezawa, S Okada, M Takano, H Negishi, H Horie, H Sawada, T Tokuhisa, E Adachi-Usami (2002) Invest Ophthalmol Vis Sci http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12091449 43: 7 2442-9

Memory B cells without somatic hypermutation are generated from Bcl6-deficient B cells

2010-05-25T05:48:56Z

After immunization with T cell-dependent antigens, the high-affinity B cells selected in germinal centers differentiate into memory B cells or long-lived antibody-forming cells. However, a role for germinal centers in development of these B lineage cells is still controversial. We show here that Bcl6-deficient B cells, which cannot develop germinal centers, differentiated into IgM and IgG1 memory ...

H Toyama, S Okada, M Hatano, Y Takahashi, N Takeda, H Ichii, T Takemori, Y Kuroda, T Tokuhisa (2002) Immunity http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12354385 17: 3 329-39

Cutting edge : a novel role for Fas ligand in facilitating antigen acquisition by dendritic cells

2010-05-25T05:48:56Z

Fas ligand (FasL)-expressing tumor cells are found to effectively mediate rejection of the coinoculated FasL negative parental cells while having no effect on the growth of histologically distinct tumor cells. These observations indicate that FasL induces a specific immune response against Ag derived from FasL-bearing tumors and suggest a possible role for FasL in tumor Ag presentation. Indeed, tu...

Y Tada, O Wang J, Y Takiguchi, K Tatsumi, T Kuriyama, S Okada, T Tokuhisa, S Sakiyama, M Tagawa (2002) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12193688 169: 5 2241-5

Identification of Nd1, a novel murine kelch family protein, involved in stabilization of actin filaments

2010-05-25T05:48:56Z

We isolated Nd1, a novel kelch family gene that encodes two forms of proteins, Nd1-L and Nd1-S. Nd1-L contains a BTB/POZ domain in its N terminus and six kelch repeats in the C terminus. Nd1-S has the BTB/POZ domain but lacks the six kelch repeats. Nd1-L but not Nd1-S mRNA is detected ubiquitously in normal mouse tissues. Nd1-L and Nd1-S proteins can form a dimer through the BTB/POZ domain. Nd1-L ...

K Sasagawa, Y Matsudo, M Kang, L Fujimura, Y Iitsuka, S Okada, T Ochiai, T Tokuhisa, M Hatano (2002) J Biol Chem http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12213805 277: 46 44140-6

Clast5/Stra13 is a negative regulator of B lymphocyte activation

2010-05-25T05:48:56Z

CD40 is a member of the tumor necrosis factor receptor family and mediates a variety of functions of B cells, including B cell survival, proliferation, immunoglobulin gene class switching, memory B cell formation, and regulation of Fas-mediated apoptosis. To begin to elucidate the molecular mechanism governing such diverse functions of CD40, we have isolated a gene from mouse splenic B cells, term...

M Seimiya, R Bahar, Y Wang, K Kawamura, Y Tada, S Okada, M Hatano, T Tokuhisa, H Saisho, T Watanabe, M Tagawa, O Wang J (2002) Biochem Biophys Res Commun http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11890681 292: 1 121-7

Binding of BAZF and Bc16 to STAT6-binding DNA sequences

2010-05-25T05:48:56Z

BAZF, a family member of Bcl6, can function as a sequence-specific transcriptional repressor. We determined BAZF-binding DNA sequence. The consensus binding sequence (CBS) of BAZF is almost the same as those of Bcl6 previously described. Three nucleotides of T, G and A at position 6, 8, and 9 in the CBS (5'-ATTCCTAGAAAG-3') are important nucleotides for binding of both BAZF and Bcl6. Since a part ...

T Hartatik, S Okada, S Okabe, M Arima, M Hatano, T Tokuhisa (2001) Biochem Biophys Res Commun http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11374866 284: 1 26-32

Testicular germ cell apoptosis in Bcl6-deficient mice

2010-05-25T05:48:56Z

Bcl6 protein has been detected in testicular germ cells, mainly spermatocytes, of normal mice, but its physiological role is largely unknown. The number of spermatozoa in the cauda epididymis of adult Bcl6-deficient (Bcl6-/-) mice is lower than that of Bcl6+/+ mice. We have found numerous apoptotic spermatocytes at the metaphase I stage with induction of Bax protein in adult Bcl6-/- testes. Develo...

S Kojima, M Hatano, S Okada, T Fukuda, Y Toyama, S Yuasa, H Ito, T Tokuhisa (2001) Development http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11092811 128: 1 57-65

Bifurcation of osteoclasts and dendritic cells from common progenitors

2010-05-25T05:48:56Z

Osteoclasts and dendritic cells are derived from monocyte/macrophage precursor cells; however, how their lineage commitment is regulated is unknown. This study investigated the differentiation pathways of osteoclasts and dendritic cells from common precursor cells at the single-cell level. Osteoclastogenesis induced by macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear ...

T Miyamoto, O Ohneda, F Arai, K Iwamoto, S Okada, K Takagi, D M Anderson, T Suda (2001) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11588053 98: 8 2544-54

Isoform-dependent interaction of BRDG1 with Tec kinase

2010-05-25T05:48:56Z

Tec is the prototype of an emerging family of protein-tyrosine kinases. Tec and Btk, another member of this family, together participate in the development of B-cell immune system. We previously identified one of the downstream messengers for human Tec kinase, BRDG1. BRDG1 is associated with Tec and becomes tyrosine-phosphorylated in B-cells by the engagement of B-cell antigen receptor (BCR). Here...

K Yokohari, Y Yamashita, S Okada, K Ohya, S Oda, M Hatano, H Mano, H Hirasawa, T Tokuhisa (2001) Biochem Biophys Res Commun http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11716489 289: 2 414-20

Progression of T cell lineage restriction in the earliest subpopulation of murine adult thymus visualized by the expression of lck proximal promoter activity

2010-05-25T05:48:56Z

The proximal promoter of lck directs gene expression exclusively in T cells. To investigate the developmental regulation of the lck proximal promoter activity and its relationship to T cell lineage commitment, a green fluorescence protein (GFP) transgenic (Tg) mouse in which the GFP expression is under the control of the proximal promoter of lck was created. In the adult GFP-Tg mice, >90% of CD4(+...

C Shimizu, H Kawamoto, M Yamashita, M Kimura, E Kondou, Y Kaneko, S Okada, T Tokuhisa, M Yokoyama, M Taniguchi, Y Katsura, T Nakayama (2001) Int Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11133839 13: 1 105-17

A new functional domain of Bcl6 family that recruits histone deacetylases

2010-05-25T05:48:56Z

The proto-oncogene Bcl6 and its family gene, BAZF, encode a sequence-specific transcriptional repressor which contains the BTB/POZ domain in NH(2)-terminal region and zinc finger motifs in COOH-terminal region. The BTB/POZ domain and the middle portion of Bcl6 and BAZF are known to display transrepressor activity. Since we have identified the identical 17-amino acid (aa) sequence in the middle por...

H Zhang, S Okada, M Hatano, S Okabe, T Tokuhisa (2001) Biochim Biophys Acta http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11583814 1540: 3 188-200

Inhibition of LPS-induced cytokines by Bcl-xL in a murine macrophage cell line

2010-05-25T05:48:56Z

The antiapoptotic molecule Bcl-xL has been implicated in the differentiation and survival of activated macrophages in inflammatory conditions. In this report, the role of Bcl-xL in LPS-induced cytokine gene expression and secretion was studied. Bcl-xL-transfected RAW 264 macrophages were protected from gliotoxin-induced apoptosis, indicating the presence of functional Bcl-xL. Overexpression of Bcl...

V Lakics, A E Medvedev, S Okada, S N Vogel (2000) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10946304 165: 5 2729-37

Impairment of B lymphopoiesis in precocious aging (klotho) mice

2010-05-25T05:48:56Z

Inactivation of the klotho gene in mice results in multiple disorders that resemble human aging after 3 weeks of age. Because hematopoiesis, especially B lymphopoiesis, is affected in humans and mice by aging, we analyzed the hematopoietic state in homozygous klotho (kl/kl) mice. The kl/kl mice showed thymic atrophy and a reduced number of splenocytes. These mice had almost the normal number of my...

S Okada, T Yoshida, Z Hong, G Ishii, M Hatano, O M Kuro, Y Nabeshima, T Tokuhisa (2000) Int Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10837414 12: 6 861-71

A single cell analysis of TCR AV24AJ18+ DN T cells

2010-05-25T05:48:56Z

The T-cell receptor (TCR) BV gene of human TCR AV24+ double-negative (DN) T cells, a novel subset of natural killer (NK) T cells, was investigated by single-cell sorting and single-cell polymerase chain reaction (PCR) methods. Seven of eleven TCR AV24+ DN T-cell clones utilized TCR BV8, three BV9, and one BV6. Six of seven TCR AV24/BV8+ DN T-cell clones had identical TCR beta and alpha chains, ind...

H Keino, I Matsumoto, S Okada, M Kurokawa, T Kato, T Tokuhisa, M Usui, M Taniguchi, K Nishioka, T Sumida (1999) Microbiol Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10480554 43: 6 577-84

Single cell analysis of T cells infiltrating labial salivary glands from patients with Sjogren's syndrome

2010-05-25T05:48:56Z

Sjogren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration into the lacrimal and salivary glands leading to symptomatic dry eyes and mouth. To analyze the function of T cells infiltrating the labial salivary glands, we analyzed T cell receptor (TCR) beta and alpha chains, the expression of various cytokine mRNAs, and apoptosis associated genes in predominant TCR BV2...

I Matsumoto, S Okada, K Kuroda, I Iwamoto, Y Saito, T Tokuhisa, K Nishioka, T Sumida (1999) Int J Mol Med http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10534575 4: 5 519-27

Prolonged expression of c-fos suppresses cell cycle entry of dormant hematopoietic stem cells

2010-05-25T05:48:56Z

The proto-oncogene c-fos was transiently upregulated in primitive hematopoietic stem (Lin-Sca-1(+)) cells stimulated with stem cell factor, interleukin-3 (IL-3), and IL-6. To investigate a role of the c-fos in hematopoietic stem cells, we used bone marrow (BM) cells from transgenic mice carrying the c-fos gene under the control of the interferon-alpha/beta-inducible Mx-promoter (Mx-c-fos), and fet...

S Okada, T Fukuda, K Inada, T Tokuhisa (1999) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9920830 93: 3 816-25

Introduction of an osteopontin gene confers the increase in B1 cell population and the production of anti-DNA autoantibodies

2010-05-25T05:48:56Z

Osteopontin (OPN) is an Arg-Gly-Asp-containing phosphoprotein that is secreted by activated T cells. The concentration of serum OPN protein is elevated in autoimmune-prone MRL-lpr mice as well as in patients with systemic lupus erythematosus. Previously, it was shown that OPN induces the polyclonal activation of B cells, resulting in the augmented production of immunoglobulin, indicating that OPN ...

J Iizuka, Y Katagiri, N Tada, M Murakami, T Ikeda, M Sato, K Hirokawa, S Okada, M Hatano, T Tokuhisa, T Uede (1998) Lab Invest http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9881952 78: 12 1523-33

c-Fos induces apoptosis in germinal center B cells

2010-05-25T05:48:56Z

We examined the role of c-Fos in the differentiation of mature B cells into IgG-producing cells using transgenic mice carrying the c-fos gene under the control of the IFN-alpha/beta-inducible Mx promoter (Mx-c-fos) or the constitutive H-2Kb promoter (H2-c-fos). Splenic B cells from Mx-c-fos mice were cultured with LPS and rIL-4, and IgG1+ B cells were developed in the culture after day 3. When IFN...

K Inada, S Okada, J Phuchareon, M Hatano, T Sugimoto, H Moriya, T Tokuhisa (1998) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9780150 161: 8 3853-61

A physiologic role of Bcl-xL induced in activated macrophages

2010-05-25T05:48:56Z

Activated macrophages produce nitric oxide (NO) that is an important effector molecule for their antimicrobial and antitumor activities. Since this NO is also toxic for themselves, they have self-defense mechanisms. To elucidate the mechanisms in a physiologic condition, expression of bcl-2 family genes were examined in peritoneal macrophages and RAW264 macrophage cell line activated with IFN-gamm...

S Okada, H Zhang, M Hatano, T Tokuhisa (1998) J Immunol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9510156 160: 6 2590-6

BAZF, a novel Bcl6 homolog, functions as a transcriptional repressor

2010-05-25T05:48:56Z

The BCL6 gene, which has been identified from the chromosomal translocation breakpoint in B-cell lymphomas, functions as a sequence-specific transcriptional repressor. We cloned a novel Bcl6-homologous gene, BAZF (encoding Bcl6-associated zinc finger protein). The predicted amino acid sequence of BAZF indicated that the BTB/POZ domain and the five repeats of the Kruppel-like zinc finger motif are ...

S Okabe, T Fukuda, K Ishibashi, S Kojima, S Okada, M Hatano, M Ebara, H Saisho, T Tokuhisa (1998) Mol Cell Biol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9632807 18: 7 4235-44

Disruption of the Bcl6 gene results in an impaired germinal center formation

2010-05-25T05:48:56Z

The Bcl6 gene has been identified from the chromosomal translocation breakpoint in B cell lymphomas, and its products are expressed highly in germinal center (GC) B cells. To investigate the function of Bcl6 in lymphocytes, we have generated RAG1-deficient mice reconstituted with bone marrow cells from Bcl6-deficient mice (Bcl6(-/-)RM). Lymphogenesis in primary lymphoid tissues of Bcl6(-/-)RM is n...

T Fukuda, T Yoshida, S Okada, M Hatano, T Miki, K Ishibashi, S Okabe, H Koseki, S Hirosawa, M Taniguchi, N Miyasaka, T Tokuhisa (1997) J Exp Med http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9236196 186: 3 439-48

An additional form of rat Bcl-x, Bcl-xbeta, generated by an unspliced RNA, promotes apoptosis in promyeloid cells

2010-05-25T05:48:56Z

The bcl-2 oncogene product delays apoptotic cell death and prolongs the cell survival. We cloned two bcl-2-related cDNAs from a rat thymus cDNA library by low stringency hybridization with a rat bcl-2 fragment as a probe. One of these, designated bcl-xalpha, was a counterpart of the human bcl-xL reported previously as a bcl-2-related gene (Boise, L. H., Gonzalez-Garcia, M., Postema, C. E. , Ding, ...

N Shiraiwa, N Inohara, S Okada, M Yuzaki, S Shoji, S Ohta (1996) J Biol Chem http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8662675 271: 22 13258-65

Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells

2010-05-25T05:48:56Z

The epitopes Tn and sialosyl-Tn are expressed on erythrocytes of individuals with a very rare blood group, who often suffer from "Tn syndrome." We surveyed expression of Tn and sialosyl-Tn in normal blood cells, malignant transformed cells, and progenitor stem cells from bone marrow (BM). An anti-Tn antibody, IE3, and an anti-sialosyl-Tn antibody, TKH2, were used in this study. TKH2 reacted with e...

K Muroi, T Suda, M Nakamura, S Okada, H Nojiri, Y Amemiya, Y Miura, S Hakomori (1994) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7903875 83: 1 84-91

Mice lacking c-fos have normal hematopoietic stem cells but exhibit altered B-cell differentiation due to an impaired bone marrow environment

2010-05-25T05:48:56Z

Mice lacking c-fos develop severe osteopetrosis with deficiencies in bone remodeling and exhibit extramedullary hematopoiesis, thymic atrophy, and altered B-cell development. In this study, we have used these mice to characterize in detail the developmental potential of hematopoietic stem cells lacking c-fos and to analyze how the lymphoid differentiation is altered. In c-fos -/- mice, B-cell numb...

S Okada, Z Q Wang, A E Grigoriadis, E F Wagner, T von Ruden (1994) Mol Cell Biol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8264605 14: 1 382-90

Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells

2010-05-25T05:48:56Z

We confirmed that murine hematopoietic stem cells express the c-kit molecule but not lymphohematopoietic lineage markers. These lineage marker-negative c-kit-positive (Lin- c-kit+) cells were further divided according to the uptake of rhodamine-123 (Rh-123). Approximately 1,000 Lin- c-kit+ rhodamine-123dull cells, which contained 4.0 +/- 1.3 and 12.5 +/- 1.9 day 8 and day 12 spleen colony-forming ...

S Okada, K Nagayoshi, H Nakauchi, S Nishikawa, Y Miura, T Suda (1993) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7681700 81: 7 1720-5

Rat stem cell factor and IL-6 preferentially support the proliferation of c-kit-positive murine hemopoietic cells rather than their differentiation

2010-05-25T05:48:56Z

We have investigated the effect of stem cell factor (SCF) alone and in combination with interleukin-3 (IL-3) or interleukin-6 (IL-6) on the proliferation and maintenance of primitive hemopoietic progenitor cells. Results from liquid preculture of either unfractionated bone marrow cells or lineage (Lin)-c-kit+ cells indicates that the combination of SCF + IL-3 results in the greatest expansion of t...

N Miura, S Okada, K M Zsebo, Y Miura, T Suda (1993) Exp Hematol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7678085 21: 1 143-9

Synergistic effect of IL-3 and IL-6 on highly enriched murine hemopoietic progenitors

2010-05-25T05:48:56Z

It has been reported that interleukin 6 (IL-6) acts on hemopoietic stem cells in synergism with interleukin 3 (IL-3), but it has not yet been clarified whether IL-6 acts directly on the stem cells or not. To investigate the mechanism of the synergism between IL-3 and IL-6, we sorted hemopoietic stem cells from untreated murine bone marrow cells using a two-laser fluorescence-activated cell sorter ...

S Okada, H Nakauchi, K Nagayoshi, M Nakamura, Y Miura, T Suda (1992) Exp Hematol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1350248 20: 5 546-51

In vivo and in vitro stem cell function of c-kit- and Sca-1-positive murine hematopoietic cells

2010-05-25T05:48:56Z

c-kit is expressed on hematopoietic stem cells and progenitor cells, but not on lymphohematopoietic differentiated cells. Lineage marker-negative, c-kit-positive (Lin-c-kit+) bone marrow cells were fractionated by means of Ly6A/E or Sca-1 expression. Lin-c-kit+Sca-1+ cells, which consisted of 0.08% of bone marrow nucleated cells, did not contain day-8 colony-forming units-spleen (CFU-S), but 80% w...

S Okada, H Nakauchi, K Nagayoshi, S Nishikawa, Y Miura, T Suda (1992) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1281687 80: 12 3044-50

In vivo stem cell function of interleukin-3-induced blast cells

2010-05-25T05:48:56Z

The treatment of mice with high doses of 5-fluorouracil (5-FU) results in an enrichment of primitive hematopoietic progenitors. Using this procedure, we obtained a new class of murine hematopoietic colonies that had very high secondary plating efficiencies in vitro and could differentiate into not only myeloid cells but also into lymphoid lineage cells. The phenotypes of interleukin-3 (IL-3) induc...

J Tsunoda, S Okada, J Suda, K Nagayoshi, H Nakauchi, K Hatake, Y Miura, T Suda (1991) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2070070 78: 2 318-22

Enrichment and characterization of murine hematopoietic stem cells that express c-kit molecule

2010-05-25T05:48:56Z

The proto-oncogene c-kit encodes a transmembrane tyrosine kinase receptor for stem cell factor (SCF). The c-kit/SCF signal is expected to have an important role in hematopoiesis. A monoclonal antibody (ACK-2) against the murine c-kit molecule was prepared. Flow cytometric analysis showed that the bone marrow cells that expressed the c-kit molecule (approximately 5%) were B220(B)-, TER119(erythroid...

S Okada, H Nakauchi, K Nagayoshi, S Nishikawa, Y Miura, T Suda (1991) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1717068 78: 7 1706-12

Effects of interleukin 3, interleukin 6, and granulocyte colony-stimulating factor on sorted murine splenic progenitor cells

2010-05-25T05:48:56Z

In order to examine the effect of recombinant growth factors on hemopoietic stem cells, these cells were enriched using wheat germ agglutinin (WGA) and monoclonal antibodies for lineage markers (Lin) such as B220, L3T4, Lyt-2, asialo GM1, Mac-1, and AL-21. Spleen colony-forming units (CFU-S) and in vitro colony-forming units were highly enriched in the fraction of WGA+Lin- spleen cells. To elimina...

S Okada, T Suda, J Suda, N Tokuyama, K Nagayoshi, Y Miura, H Nakauchi (1991) Exp Hematol http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1703493 19: 1 42-6

A stimulatory effect of recombinant murine interleukin-7 (IL-7) on B-cell colony formation and an inhibitory effect of IL-1 alpha

2010-05-25T05:48:56Z

Using a clonal culture system, we investigated the lymphohematopoietic effects of recombinant interleukin-7 (IL-7) obtained from conditioned media of transfected COS 1 cells. IL-7 alone acted on murine bone marrow cells and supported the formation of B-cell colonies. These colony cells were positive for B220, and some of them were also found to have either IgM or Thy-1. B220+, IgM- cells, but not ...

T Suda, S Okada, J Suda, Y Miura, M Ito, T Sudo, S Hayashi, S Nishikawa, H Nakauchi (1989) Blood http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2804342 74: 6 1936-41

IL-34 and M-CSF share the receptor Fms but are not identical in biological activity and signal activation

2010-05-25T05:48:56Z

Macrophage colony-stimulating factor (M-CSF) regulates the production, survival and function of macrophages through Fms, the receptor tyrosine kinase. Recently, interleukin-34 (IL-34), which shares no sequence homology with M-CSF, was identified as an alternative Fms ligand. Here, we provide the first evidence that these ligands indeed resemble but are not necessarily identical in biological activ...

T Chihara, S Suzu, R Hassan, N Chutiwitoonchai, M Hiyoshi, K Motoyoshi, F Kimura, S Okada () Cell Death Differ http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20489731 :

Cepharanthine exerts antitumor activity on cholangiocarcinoma by inhibiting NF-kappaB

2010-05-25T05:48:56Z

Cholangiocarcinoma (CCA) is a major cause of cancer deaths in northeast Thailand. It is aggressive, highly metastatic, and responds poorly to traditional chemotherapy. We demonstrated the potential for Cepharanthine (CEP), a biscoclaurine alkaloid extracted from Stephania cepharantha, to treat CCA. CEP significantly inhibited growth of human CCA cell lines in a dose- and time-dependent manner, reg...

W Seubwai, K Vaeteewoottacharn, M Hiyoshi, S Suzu, A Puapairoj, C Wongkham, S Okada, S Wongkham () Cancer Sci http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20412118 :