http://publicationslist.org/data/silvia.ravera/atom.xml Silvia Raverahttp://publicationslist.org/silvia.ravera$basepathfavicon.icoRecent additions to Silvia Ravera's PublicationsList.org pagehttp://publicationslist.org/publications.png2010-05-04T09:37:00Z http://publicationslist.org/silvia.ravera/refid16 2010-05-04T09:35:16Z

We describe a procedure for the labeling of membranous vesicular purified subcellular fractions, to image them, typically by confocal laser scanning microscopy. Being intracellular organelles, these fractions, once purified cannot be attached to glass slides as for cells. Fractions are labeled "in batch" without prior embedding or freezing. Each labeling step performed by passages of resuspension/...<br/><br/>Isabella Panfoli, Daniela Calzia, Silvia Ravera, Paolo Bianchini, Alberto Diaspro (2010) <i>Microsc Res Tech</i> <i></i> <i></i> : <br/>

http://publicationslist.org/silvia.ravera/refid18 2010-05-04T09:35:16Z

The effects of extremely low frequency magnetic fields (ELF-MF) on acetylcholinesterase (AChE) activity of synaptosomal membranes were investigated. Sinusoidal fields with 50 Hz frequency and different amplitudes caused AChE activity to decrease about 27% with a threshold of about 0.74 mT. The decrease in enzymatic activity was independent of the time of permanence in the field and was completely ...<br/><br/>Silvia Ravera, Bruno Bianco, Carlo Cugnoli, Isabella Panfoli, Daniela Calzia, Alessandro Morelli, Isidoro M Pepe (2010) <i>Bioelectromagnetics</i> <i></i> <i></i> 31: 4 270-276<br/>

http://publicationslist.org/silvia.ravera/refid17 2010-05-04T09:35:16Z

The mammalian hormone, leptin, is mainly synthesized in adipose tissue along with other tissues. Leptin plays a role in numerous processes such as in the control of food intake, homeostasis, immune function and reproduction. In this study, we detected and localized leptin immunoreactivity to the muscle of early juvenile sea bass (Dicentrarchus labrax) by Western blot analysis and immunohistochemis...<br/><br/>Chiara Gambardella, Sara Ferrando, Lorenzo Gallus, Silvia Ravera, Paolo Bianchini, Paola Ramoino, Salvatore Fasulo, Grazia Tagliafierro (2010) <i>Microsc Res Tech</i> <i></i> <i></i> : <br/>

http://publicationslist.org/silvia.ravera/refid13 2010-05-04T09:35:16Z

The disks of the vertebrate retinal rod Outer Segment (OS), devoid of mitochondria, are the site of visual transduction, a very energy demanding process. In a previous proteomic study we reported the expression of the respiratory chain complexes I-IV and the oxidative phosphorylation Complex V (F(1)F(0)-ATP synthase) in disks. In the present study, the functional localization of these proteins in ...<br/><br/>Isabella Panfoli, Daniela Calzia, Paolo Bianchini, Silvia Ravera, Alberto Diaspro, Giovanni Candiano, Angela Bachi, Massimiliano Monticone, Maria Grazia Aluigi, Stefano Barabino, Giovanni Calabria, Maurizio Rolando, Carlo Tacchetti, Alessandro Morelli, Isidoro M Pepe (2009) <i>Int J Biochem Cell Biol</i> <i></i> <i></i> 41: 12 2555-2565<br/>

http://publicationslist.org/silvia.ravera/refid14 2009-09-04T12:44:26Z

Even though brain represents only 2-3% of the body weight, it consumes 20% of total body oxygen, and 25% of total body glucose. This sounds surprising, in that mitochondrial density in brain is low, while mitochondria are thought to be the sole site of aerobic energy supply. These data would suggest that structures other than mitochondria are involved in aerobic ATP production. Considering that a ...<br/><br/>Silvia Ravera, Isabella Panfoli, Daniela Calzia, Maria Grazia Aluigi, Paolo Bianchini, Alberto Diaspro, Gianluigi Mancardi, Alessandro Morelli (2009) <i>Int J Biochem Cell Biol</i> <i></i> <i></i> 41: 7 1581-1591<br/>

http://publicationslist.org/silvia.ravera/refid15 2009-09-04T12:44:51Z

The mechanosensory lateral line (LL) is involved in many fish and amphibian behaviors, however little is known about the molecules involved in the signal transmission. Neuropeptide Y (NPY) has a number of functions in vertebrate physiology and also plays important roles in different sensory systems. The Antarctic nototheniods are a monophyletic radiation of fishes that have evolved under the extre...<br/><br/>Massimiliano Bottaro, Sara Ferrando, Silvia Ravera, Marino Vacchi, Lorenzo Gallus, Chiara Gambardella, Grazia Tagliafierro (2009) <i>Neurosci Lett</i> 458: 1 37-42<br/>

http://publicationslist.org/silvia.ravera/refid19 2010-05-04T09:35:16Z

The interaction of direct electric current (dc) and proteins is a little explored topic. We had reported that exposure of Crotalus atrox venom to dc caused irreversible inactivation of phospholipase A(2) and metalloprotease and that the eukaryote adenylate kinases (AK) precipitate in nondenaturing gel electrophoresis. AK1 displays an elevated percent difference of CHarged versus POlar amino acid c...<br/><br/>Daniela Calzia, Isabella Panfoli, Silvia Ravera, Elisa Dazzi, Simona Gandolfo, Isidoro Mario Pepe, Laura Vergani, Alessandro M Morelli (2009) <i>J Biochem Mol Toxicol</i> <i></i> <i></i> 23: 5 309-317<br/>

http://publicationslist.org/silvia.ravera/refid20 2010-05-04T09:35:16Z

In the olfactory and vomeronasal systems of vertebrates, the morphology of the receptor neurons, the receptor gene family they express, the G-protein coupled with the receptor (in particular the G-protein alpha subunit), and their projection to the olfactory bulb are correlated. Much information about this complicated system have been collected in different groups, but nothing is known about Chond...<br/><br/>Sara Ferrando, Chiara Gambardella, Silvia Ravera, Sergio Bottero, Tiziana Ferrando, Lorenzo Gallus, Valentina Manno, Amir P Salati, Paola Ramoino, Grazia Tagliafierro (2009) <i>Anat Rec (Hoboken)</i> <i></i> <i></i> 292: 11 1771-1779<br/>

http://publicationslist.org/silvia.ravera/refid11 2009-01-30T11:09:13Z

The vertebrate retina is an array of "narrow-capture" photoreceptive elements of diverse cellular types that allow the fine spatial resolution characteristic of vision. Imaging of photoreceptors and of the whole retina has been previously reported; however, both were achieved exclusively after fixation. We report our development of a new technique for imaging live bovine retinas ex vivo. Using thi...<br/><br/>Paolo Bianchini, Daniela Calzia, Silvia Ravera, Giovanni Candiano, Angela Bachi, Alessandro Morelli, Maurizio Bruschi, Isidoro M Pepe, Alberto Diaspro, Isabella Panfoli (2008) <i>J Biomed Opt</i> <i></i> <i></i> 13: 5 <br/>

http://publicationslist.org/silvia.ravera/refid12 2009-01-30T11:09:13Z

The initial events of vision at low light take place in vertebrate retinal rods. The rod outer segment consists of a stack of flattened disks surrounded by the plasma membrane. A list of the proteins that reside in disks has not been achieved yet. We present the first comprehensive proteomic analysis of purified rod disks, obtained by combining the results of two-dimensional gel electrophoresis se...<br/><br/>Isabella Panfoli, Luca Musante, Angela Bachi, Silvia Ravera, Daniela Calzia, Angela Cattaneo, Maurizio Bruschi, Paolo Bianchini, Alberto Diaspro, Alessandro Morelli, Isidoro M Pepe, Carlo Tacchetti, Giovanni Candiano (2008) <i>J Proteome Res</i> <i></i> <i></i> 7: 7 2654-2669<br/>

http://publicationslist.org/silvia.ravera/refid5 2008-02-11T13:27:01Z

Adenylate kinases (AKs) are ubiquitous monomeric phosphotransferases catalyzing the reversible reaction, AMP + MgATP = ADP + MgADP, which plays a pivotal role in the energetic metabolism. In vertebrates, six AK isoforms are known. In this work, we report the detection of many AK isoforms directly on gel or NC after separation by denaturing electrophoresis and electroblotting, by an optimized proto...<br/><br/>Silvia Ravera, Daniela Calzia, Isabella Panfoli, Isidoro Mario Pepe, Alessandro Morelli (2007) <i>Electrophoresis</i> <i></i> <i></i> 28: 3 291-300<br/>

http://publicationslist.org/silvia.ravera/refid3 2008-02-11T13:27:01Z

To achieve our aim of understanding the interactions between direct current and enzymes in solution, we exposed reconstituted Crotalus atrox venom to direct electric current by immersing two platinum thread electrodes connected to a voltage generator (between 0 and 8 V) into a reaction mixture for a few seconds. Then, we assayed the residual activity of phospholipases A(2) (PLA(2)),metalloproteina...<br/><br/>Isabella Panfoli, Silvia Ravera, Daniela Calzia, Elisa Dazzi, Simona Gandolfo, Isidoro M Pepe, Alessandro Morelli (2007) <i>J Biochem Mol Toxicol</i> <i></i> <i></i> 21: 1 7-12<br/>

http://publicationslist.org/silvia.ravera/refid2 2008-02-11T13:27:01Z

Adenylate kinases (AKs) are ubiquitous phosphotransferases that contribute to homeostasis of adenine nucleotide composition in cells. Six AK isoforms were found in vertebrates. We report that soluble AK isoform 1 is expressed in the cytosol of bovine retina consistently devoid of rod outer segments. Immunoblotting analysis with a polyclonal antibody raised against soluble adenylate kinase and subs...<br/><br/>Silvia Ravera, Luca Musante, Daniela Calzia, Isabella Panfoli, Maurizio Bruschi, Giovanni Candiano, Isidoro Mario Pepe, Alessandro Morelli (2007) <i>Curr Eye Res</i> <i></i> <i></i> 32: 3 249-257<br/>

http://publicationslist.org/silvia.ravera/refid10 2008-02-15T11:21:28Z

Adenylate kinase is a monomeric enzyme, found in all organisms. The enzyme is involved in the interconversion of adenine nucleotides, playing a pivotal role in the energetic metabolism. In vertebrates, six isoforms of adenylate kinase are known. Previously, we identified the cytosolic adenylate kinase isoform 1 in bovine retina consistently devoid of rod outer segments. In this paper the kinet...<br/><br/>D. Calzia, S. Ravera, A. Morelli, I.Panfoli. (2007) <i>Expermental Biology Reports</i> 1: 1 <br/>

http://publicationslist.org/silvia.ravera/refid1 2008-02-11T13:27:01Z

Vertebrate retinal rod outer segment disks house the proteins involved in the phototransduction cascade that converts light into neuronal signal. We develop a technique for the immunofluorescent labeling of osmotically intact isolated rod outer segment disks for confocal laser scanning microscopy imaging. Osmotically intact Ficoll-flotation isolated bovine disks are directly labeled with antibodie...<br/><br/>Silvia Ravera, Daniela Calzia, Paolo Bianchini, Alberto Diaspro, Isabella Panfoli (2007) <i>J Biomed Opt</i> <i></i> <i></i> 12: 5 <br/>

http://publicationslist.org/silvia.ravera/refid4 2008-02-11T13:27:01Z

PURPOSE: Calcium ions play a pivotal role in phototransduction. In this study, the presence and functional role of the adenosine diphosphoribosyl (ADPR)-cyclase-cyclic ADP-ribose (cADPR) system in bovine retinal rod outer segments (ROS) was investigated. METHODS: A Ca(2+) release from osmotically intact ROS discs elicited by cADPR was studied in the presence of the Ca(2+) tracer fluo-3. Endogenous...<br/><br/>Isabella Panfoli, Silvia Ravera, Andrea Fabiano, Raffaella Magrassi, Alberto Diaspro, Alessandro Morelli, Isidoro M Pepe (2007) <i>Invest Ophthalmol Vis Sci</i> <i></i> <i></i> 48: 3 978-984<br/>

http://publicationslist.org/silvia.ravera/refid6 2008-02-11T13:28:15Z

Exposure of fertilized eggs of the sea urchin Paracentrotus lividus to an electromagnetic field of 75-Hz frequency and low amplitudes (from 0.75 to 2.20 mT of magnetic component) leads to a dramatic loss of synchronization of the first cell cycle, with formation of anomalous embryos linked to irregular separation of chromatids during the mitotic events. Because acetylcholinesterase (ACHE) is thoug...<br/><br/>Silvia Ravera, Carla Falugi, Daniela Calzia, Isidoro M Pepe, Isabella Panfoli, Alessandro Morelli (2006) <i>Biol Reprod</i> <i></i> <i></i> 75: 6 948-953<br/>

http://publicationslist.org/silvia.ravera/refid7 2008-02-11T13:28:15Z

The effects of extremely low frequency electromagnetic fields of 75 Hz were studied on different membrane-associated enzymes. Only the activities of three enzymes out of seven exposed to the field decreased approximately of about 54-61% with field amplitudes above a threshold of 73-151 microT depending on the enzyme. The same field had no effect on the activities of either integral membrane enzyme...<br/><br/>A Morelli, S Ravera, I Panfoli, I M Pepe (2005) <i>Arch Biochem Biophys</i> <i></i> <i></i> 441: 2 191-198<br/>

http://publicationslist.org/silvia.ravera/refid9 2008-02-11T13:28:15Z

Adenylate kinase activity in rod outer segment membranes of bovine retina decreased of about 55% when exposed to an extremely low frequency electromagnetic field of 75 Hz and 250 microT. The effect was independent of the time of permanence in the field. Negligible effects of the field were found on the enzymatic activity of a soluble isoform of adenylate kinase or of rod outer segment membranes so...<br/><br/>S Ravera, E Repaci, A Morelli, I M Pepe, R Botter, D Beruto (2004) <i>Bioelectrochemistry</i> <i></i> <i></i> 63: 1-2 317-320<br/>

http://publicationslist.org/silvia.ravera/refid8 2008-02-11T13:28:15Z

Extremely low frequency electromagnetic fields (ELF-EMFs) of 75 Hz with amplitudes above a threshold of about 125 microT have a dramatic effect on the adenylate kinase (AK) activity of the rod outer segment (ROS) membranes. In fact, the ATP production by ROS membranes or by purified disk membranes placed in the field decreased by approximately 54%. The decrease in enzymatic activity was independen...<br/><br/>S Ravera, E Repaci, A Morelli, I M Pepe, R Botter, D Beruto (2004) <i>Bioelectromagnetics</i> <i></i> <i></i> 25: 7 545-551<br/>