younousse saidi's Publications List

The Membrane-Associated Transient Receptor Potential Vanilloid Channel is the Central Heat Shock Receptor Controlling the Cellular Heat Shock Response in Epithelial Cells.

2013-03-07T20:52:05Z

Z Bromberg, P Goloubinoff, Y Saidi, Y G Weiss (2013) PlosOne 8: 2 e57149

Function and evolution of 'green' GSK3/Shaggy-like kinases

2012-04-25T11:25:56Z

Glycogen synthase kinase 3 (GSK3) proteins, also known as SHAGGY-like kinases, have many important cell signalling roles in animals, fungi and amoebae. In particular, GSK3s participate in key developmental signalling pathways and also regulate the cytoskeleton. GSK3-encoding genes are also present in all land plants and in algae and protists, raising questions about possible ancestral functions in...

Saidi Y, Hearn TH and Coates JC (2012) Trends in Plant Science 7: 1 39–46

Heat shock response in photosynthetic organisms: membrane and lipid connections

2012-04-25T11:25:20Z

The ability of photosynthetic organisms to adapt to increases in environmental temperatures is becoming more important with climate change. Heat stress is known to induce heat-shock proteins (HSPs) many of which act as chaperones. Traditionally, it has been thought that protein denaturation acts as a trigger for HSP induction. However, increasing evidence has shown that many stress events cause HS...

Horváth I, Glatz A, Nakamoto H, Mishkind ML, Munnik T, Saidi Y, Goloubinoff P, Haward J. and Vigh L (2012) Progress in Lipid Research 51: 3 208–220

ARABIDILLO gene homologues in basal land plants: species-specific gene duplication and likely functional redundancy

2013-01-25T13:43:19Z

Moody LA, Saidi Y, Smiles EJ, Bradshaw SJ, Meddings M, Winn PJ and Coates JC. (2012) Planta 236: 6 1927

Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance

2013-01-25T13:44:51Z

Finka A, Henriquez AF, Maathuis FJM, Saidi Y and Goloubinoff P. (2012) Plant Cell 24: 8 3333

Heat perception and signalling in plants: a tortuous path to thermotolerance

2011-05-16T18:49:11Z

An accurate assessment of the rising ambient temperature by plant cells is crucial for the timely activation of various molecular defences before the appearance of heat damage. Recent findings have allowed a better understanding of the early cellular events that take place at the beginning of mild temperature rise, to timely express heat-shock proteins (HSPs), which will, in turn, confer thermotol...

Y Saidi, A Finka and P Goloubinoff (2011) New Phytologist 190: 3 556–565

Plants and the Earth System – past events and future challenge.

2011-01-11T18:39:03Z

J.C Coates, L.A Moody and Y Saidi (2011) New Phytologist 189: 2 370–373

Rice cDNA Encoding PROLM is Capable of Rescuing Salt Sensitive Yeast Phenotypes G19 and Axt3K from Salt Stress.

2011-12-15T14:53:27Z

Senadheera P., Saidi Y., Maathuis FJM. (2011) WORLD ACADEMY OF SCIENCE, ENGINEERING AND TECHNOLOGY (59) pp981

Plant Armadillo-related proteins have conserved functions involved in abscisic acid response.

2011-12-15T14:55:15Z

Y. Saidi, L. A. Moody, D. J. Gibbs & J. C. Coates. (2011) MOSS2011 meeting, Black Forest, Germany,

Armadillo repeat proteins in the moss Physcomitrella patens: structure, stability and subcellular localisation.

2010-10-27T09:54:18Z

Y.Saidi, L. A. Moody, S. Bradshaw & J.C. Coates. (2010) 25th New Phytologist Symposium: Colonization of the terrestrial environment, Bristol, United Kingdom, September

Membrane lipid composition affects plant heat sensing and modulates Ca2+-dependent heat shock response

2011-06-29T10:48:33Z

Understanding how plants sense and respond to heat stress is central to improve crop tolerance and productivity. Recent findings in Physcomitrella patens demonstrated that the controlled passage of calcium ions across the plasma membrane regulates the heat shock response (HSR). To investigate the effect of membrane lipid composition on the plant HSR, we acclimated P. patens to a slightly elevated...

Y Saidi, M Peter, A Finka, C Cicekli, L Vigh and P Goloubinoff (2010) plant signaling and behavior 5: 12 1530-3

The CaMV 35S promoter has a weak expression activity in dark grown tissues of moss Physcomitrella patens

2011-01-11T18:40:45Z

The constitutive Cauliflower Mosaic Virus 35S promoter (CaMV 35S) is widely used as a tool to express recombinant proteins in plants, but with different success. We previously showed that the expression of an F-actin marker, GFP-talin, in Physcomitrella patens using the CaMV 35S promoter failed to homogenously label moss tissues. Here, we show a significant diminution of the GFP fluorescence in da...

Y Saidi, DG. Schaefer, P Goloubinoff, JP Zryd and A Finka (2009) Plant signaling and behavior 4: 5 457-459

The Heat Shock Response in Moss Plants Is Regulated by Specific Calcium-Permeable Channels in the Plasma Membrane

2012-02-03T12:40:05Z

Land plants are prone to strong thermal variations and must therefore sense early moderate temperature increments to induce appropriate cellular defenses, such as molecular chaperones, in anticipation of upcoming noxious temperatures. To investigate how plants perceive mild changes in ambient temperature, we monitored in recombinant lines of the moss Physcomitrella patens the activation of a heat-...

Y Saidi, A Finka, M Muriset, Z Bromberg, Y. G. Weiss, F. J.M. Maathuis and P Goloubinoff (2009) Plant Cell 21: 9 2829-2843

A novel family of cation/calcium exchangers impacts on osmotic and drought stress tolerance in Arabidopsis thaliana

2010-10-27T09:53:29Z

Y. Saidi, D Sanders and F.J.M Maathuis. (2008) SEB Plant Transport Group Meeting. Manchester, United Kingdom, September

The knock-out of ARP3a gene affects F-actin cytoskeleton organization altering cellular tip growth, morphology and development in moss Physcomitrella patens.

2009-03-27T15:17:17Z

The seven subunit Arp2/3 complex is a highly conserved nucleation factor of actin microfilaments. We have isolated the genomic sequence encoding a putative Arp3a protein of the moss Physcomitrella patens. The disruption of this ARP3A gene by allele replacement has generated loss-of-function mutants displaying a complex developmental phenotype. The loss-of function of ARP3A gene results in shortene...

A Finka, Y Saidi, P Goloubinoff, JM Neuhaus, JP Zrÿd, DG Schaefer (2008) Cell Motil Cytoskeleton 65: 10 769-784

Specific plasma membrane calcium channels control the plant heat-shock response

2009-03-27T15:01:08Z

Y. Saidi and P. Goloubinoff. (2008) The annual international symposium for moss experimental research (FESPB)

The plant heat-shock response is controlled by specific calcium channels in the plasma membrane

2009-03-27T18:57:42Z

Y. Saidi and P. Goloubinoff (2008) Non-Ionising Radiation - Health and Environment

Activation of the heat shock response in plants by chlorophenols: transgenic Physcomitrella patens as a sensitive biosensor for organic pollutants.

2009-03-27T15:17:50Z

The ability to detect early molecular responses to various chemicals is central to the understanding of biological impact of pollutants in a context of varying environmental cues. To monitor stress responses in a model plant, we used transgenic moss Physcomitrella patens expressing the beta-glucuronidase reporter (GUS) under the control of the stress-inducible promoter hsp17.3B. Following exposure...

Y Saidi, M Domini, F Choy, JP Zryd, JP Schwitzguebel, P Goloubinoff (2007) Plant Cell Environ 30: 6 753-763

How plants sense heat: the triggering mechanism of the heat-shock response

2009-03-27T14:58:08Z

Y. Saidi and P. Goloubinoff (2007) 16th Swiss Plant Molecular and Cell Biology Conference pp25

Activation of the heat-shock response in plants by chlorophenols: transgenic Physcomitrella patens as a sensitive biosensor for organic pollutant

2009-08-14T14:17:23Z

Y. Saidi, M. Domini, F. Choy, J-P. Schwitzguebel and P. Goloubinoff (2007) Vacuoles and plastids: development and functions pp21

Recombinant Physcomitrella patens as a sensitive tool to study heat sensing and heat-shock signal transduction in plants

2009-03-28T02:44:41Z

When exposed to heat stress, plants display a particular set of cellular and molecular responses, such as chaperones expression, which are highly conserved in all organisms. In chapter 1, I studied the ability of heat shock genes to become transiently and abundantly induced under various temperature regimes. To this aim, I designed a highly sensitive heat-shock dependent conditional gene expressio...

Younousse Saidi (2007)

In vivo visualization of F-actin structures during the development of the moss Physcomitrella patens.

2009-03-27T15:18:19Z

* The 'in planta' visualization of F-actin in all cells and in all developmental stages of a plant is a challenging problem. By using the soybean heat inducible Gmhsp17.3B promoter instead of a constitutive promoter, we have been able to label all cells in various developmental stages of the moss Physcomitrella patens, through a precise temperature tuning of the expression of green fluorescent pro...

A Finka, DG Schaefer, Y Saidi, P Goloubinoff, JP Zrÿd (2007) New Phytol 174: 1 63-76

The moss Physcomitrella patens as a tool for plant biotechnology and stress-sensing research

2009-03-27T14:46:47Z

Y. Saidi, M. Domini, A. Finka, D. Schaefer and P. Goloubinoff (2006) Deuxieme Congres International de Biochimie pp71

The use of the heat-inducible promoter to modulate gene expression in P. paten

2009-08-14T14:17:31Z

Y. Saidi, A. Finka, D. Schaefer, J-P. Zryd and P. Goloubinoff (2006) The 9th Annual Moss International Conference

Controlled expression of recombinant proteins in Physcomitrella patens by a conditional heat-shock promoter: a tool for plant research and biotechnology.

2009-03-27T15:18:47Z

The ability to express tightly controlled amounts of endogenous and recombinant proteins in plant cells is an essential tool for research and biotechnology. Here, the inducibility of the soybean heat-shock Gmhsp17.3B promoter was addressed in the moss Physcomitrella patens, using beta-glucuronidase (GUS) and an F-actin marker (GFP-talin) as reporter proteins. In stably transformed moss lines, Gmhs...

Y Saidi, A Finka, M Chakhporanian, JP Zrÿd, DG Schaefer, P Goloubinoff (2005) Plant Mol Biol 59: 5 697-711

Transgenic Physcomitrella patens carrying a heat inducible promoter: powerful tool for controlled expression of recombinant proteins and useful system for heat shock response analysis

2009-08-14T14:17:39Z

Y. Saidi, A. Finka, J.P. Zryd, D. Schaefer, P. Goloubinoff (2005) The 8th Annual Moss International Conference pp22

High conditional expression of recombinant protein in Physcomitrella patens using a soybean heat-shock promoter

2009-03-27T19:00:36Z

Y. Saidi, A. Finka, D. Schaefer, J.P. Zryd, P. Goloubinoff. (2004) The 7th Annual Moss International Conference pp 48

Tackling the problem of thermal versus non thermal biological effects of high frequency electromagnetic radiations

2009-03-27T14:45:10Z

J.P. Zryd, E. Alasonati, P Goloubinoff, Y. Saidi, P. Zweiacker, F. Rachidi (2004) Progress in Electromagnetics Research Symposium (PIERS) pp181-184