http://publicationslist.org/data/yamagata/atom.xml Hiroshi Yamagatahttp://publicationslist.org/yamagata$basepathfavicon.icoRecent additions to Hiroshi Yamagata's PublicationsList.org pagehttp://publicationslist.org/publications.png2008-11-15T05:58:19Z http://publicationslist.org/yamagata/refid20 2008-11-13T05:22:23Z

P450 (cytochrome P450) enzymes catalyse the mono-oxygenation of a wide range of compounds such as steroids, fatty acids, vitamins and drugs. In the present paper we demonstrate a system for bioconverting diverse compounds [flavanone, DHEA (dehydroepiandrosterone) and 7-ethoxycoumarin] using P450 species expressed in Escherichia coli. First, we expressed four P450 species: rabbit CYP2B (P450 family...<br/><br/>Tomohide Uno, Sota Okamoto, Satoko Masuda, Atsushi Itoh, Yuichi Uno, Masahiko Nakamura, Kengo Kanamaru, Hiroshi Yamagata, Hiromasa Imaishi (2008) <i>Biotechnol Appl Biochem</i> <i></i> <i></i> 50: Pt 4 165-171<br/>

http://publicationslist.org/yamagata/refid17 2008-11-13T05:22:23Z

The small GTPases known as Rab proteins are key regulators of membrane trafficking. We used RT-PCR to isolate cDNA clones of insect-specific Rab proteins (BRabN1 and BRabN2) showing low homology with known Rab proteins from other animals, from mRNA of Bombyx mori. These 2 Rabs were produced in Escherichia coli and purified. BRabN1 bound [(3)H]-GDP and [(35)S]-GTPgammaS with dissociation constants ...<br/><br/> Uno, Moriwaki, Nakamura, Matsubara, Yamagata, Kanamaru, Takagi (2008) <i>Arch Insect Biochem Physiol</i> <i></i> <i></i> : <br/>

http://publicationslist.org/yamagata/refid18 2008-11-13T05:22:23Z

The cold-active protein tyrosine phosphatase found in psychrophilic Shewanella species exhibits high catalytic efficiency at low temperatures as well as low thermostability, both of which are characteristics shared by many cold-active enzymes. The structure of cold-active protein tyrosine phosphatase is notable for the presence of three hydrophobic sites (termed the CA, Zn-1 and Zn-2 sites) behind...<br/><br/>Hiroki Tsuruta, Bunzo Mikami, Chiaki Yamamoto, Hiroshi Yamagata (2008) <i>FEBS J</i> <i></i> <i></i> 275: 17 4317-4328<br/>

http://publicationslist.org/yamagata/refid19 2008-11-13T05:22:23Z

We indicated that two P450s (1A9 and 1C1) from Japanese eel (Anguilla japonica) metabolized 7-ethoxycoumarin, 7-ethoxyresorufin, and flavanone. At first, we constructed expression vectors for two types of P450 (1A9 and 1C1). The reduced CO-difference spectra of Escherichia coli cells transformed with these plasmids showed Soret peaks (450 nm) that were typical of P450s. We performed bioconversion ...<br/><br/>Tomohide Uno, Sota Okamoto, Satoko Masuda, Hiromasa Imaishi, Masahiko Nakamura, Kengo Kanamaru, Hiroshi Yamagata, Mohamed A H El-Kady, Yoshio Kaminishi, Takao Itakura (2008) <i>Comp Biochem Physiol C Toxicol Pharmacol</i> <i></i> <i></i> 147: 3 278-285<br/>

http://publicationslist.org/yamagata/refid1 2008-11-13T04:53:28Z

Cyclic GMP (cGMP) is an important signaling molecule that controls a range of cellular functions. So far, however, only a few genes have been found to be regulated by cGMP in higher plants. We investigated the cGMP-responsiveness of several genes encoding flavonoid-biosynthetic enzymes in soybean (Glycine max L.) involved in legume-specific isoflavone, phytoalexin and anthocyanin biosynthesis, suc...<br/><br/> Suita, Kiryu, Sawada, Mitsui, Nakagawa, Kanamaru, Yamagata (2008) <i>Planta</i> <i></i> <i></i> : <br/>

http://publicationslist.org/yamagata/refid21 2008-11-13T05:22:23Z

The Rab family of small GTPases are key regulators of membrane trafficking. Partially purified Rab8 from Bombyx mori (BRab8) was phosphorylated by protein kinase C in mammalian cells in vitro. To determine which of the seven serines and four threonines are phosphorylated, we generated deletion and site-directed mutants of BRab8, inserted them in Escherichia coli, partially purified the encoded fus...<br/><br/>Tomohide Uno, Takuya Nakada, Sota Okamaoto, Masahiko Nakamura, Mamoru Matsubara, Hiromasa Imaishi, Hiroshi Yamagata, Kengo Kanamaru, Michihiro Takagi (2007) <i>Arch Insect Biochem Physiol</i> <i></i> <i></i> 66: 2 89-97<br/>

http://publicationslist.org/yamagata/refid22 2008-11-13T05:22:23Z

We developed a system for bioconverting diverse compounds using P450s produced in Escherichia coli. Vectors for the expressing various P450 cDNAs quickly and easily in E. coli were developed by using several restriction enzyme sites. Three types of P450 (2C2, 2C29, and 2D22) were produced using these plasmids. Substrates were directly added to the incubation medium and metabolized. To obtain pure ...<br/><br/>Tomohide Uno, Atsushi Nakao, Satoko Masuda, Yuuki Taniguchi, Kengo Kanamaru, Hiroshi Yamagata, Masahiko Nakamura, Hiromasa Imaishi, Kiyoharu Oono (2006) <i>J Ind Microbiol Biotechnol</i> <i></i> <i></i> 33: 12 1043-1050<br/>

http://publicationslist.org/yamagata/refid23 2008-11-13T05:22:23Z

The complete nucleotide sequences of the cDNA and its gene that encode a bifunctional alpha-amylase/subtilisin inhibitor of rice (Oryza sativa L.) (RASI) were analyzed. RASI cDNA (939 bp) encoded a 200-residue polypeptide with a molecular mass of 21,417 Da, including a signal peptide of 22 amino acids. Sequence comparison and phylogenetic analysis showed that RASI is closely related to alpha-amyla...<br/><br/>Teruyuki Yamasaki, Masaki Deguchi, Toshiko Fujimoto, Takehiro Masumura, Tomohide Uno, Kengo Kanamaru, Hiroshi Yamagata (2006) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 70: 5 1200-1209<br/>

http://publicationslist.org/yamagata/refid24 2008-11-13T05:22:23Z

A cDNA clone encoding methyl DNA binding domain-containing protein (bMBD2/3) was obtained by homology searches using a Bombyx mori fat body cDNA library. The cDNA encoded a polypeptide with 249 amino acids sharing 54% similarity with the methyl DNA binding protein from Drosophila melanogaster. To characterize the biochemical properties of bMBD2/3, the clone was expressed in Escherichia coli as His...<br/><br/>Tomohide Uno, Yuka Nomura, Masahiko Nakamura, Atsushi Nakao, Shoji Tajima, Kengo Kanamaru, Hiroshi Yamagata, Yousuke Iwanaga (2005) <i>J Insect Sci</i> <i></i> <i></i> 5: <br/>

http://publicationslist.org/yamagata/refid13 2008-11-13T05:06:03Z

Protein-tyrosine phosphatase [EC 3.1.3.48] from a psychrophile, Shewanella sp. shows high activity at low temperatures and has the conserved amino acid sequence of protein-Ser/Thr-phosphatases. Site-directed mutagenesis with the conserved amino acid residues indicated that His148 could be important as a general acid catalyst and Asp115 assists the protonation with His148 of the leaving group of a ...<br/><br/>Hiroki Tsuruta, Jun Tamura, Hiroshi Yamagata, Yasuo Aizono (2004) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 68: 2 440-443<br/>

http://publicationslist.org/yamagata/refid25 2008-11-13T05:26:19Z

The signal transduction processes involved in the regulation of SAMDC gene expression by blue and red light were examined using pharmacological inhibitors of signalling pathways. Calcium and calmodulin positively regulated SAMDC gene expression in red light, whereas in blue light they regulated negatively. These results indicate that calcium homeostasis is involved in both red and blue light induc...<br/><br/>Izumi Yoshida, Hiroshi Yamagata, Eiji Hirasawa (2002) <i>J Exp Bot</i> <i></i> <i></i> 53: 373 1525-1529<br/>

http://publicationslist.org/yamagata/refid14 2008-11-13T05:06:03Z

A psychrophilic alkaline phosphatase (EC 3.1.3.1) from Shewanella sp. is a cold-active enzyme that has high catalytic activity at low temperature [Ishida et al. (1998) Biosci. Biotechnol. Biochem., 62, 2246-2250]. Here, we identified the nucleotide sequence of a gene encoding the enzyme after cloning with the polymerase chain reaction (PCR) and inverted PCR techniques. The deduced amino acid seque...<br/><br/>Takeshi Murakawa, Hiroshi Yamagata, Hiroki Tsuruta, Yasuo Aizono (2002) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 66: 4 754-761<br/>

http://publicationslist.org/yamagata/refid15 2008-11-13T05:06:03Z

Cucumisin, a subtilisin-like serine protease, is expressed at high levels in the fruit of melon (Cucumis melo L.) and accumulates in the juice. We investigated roles of the promoter regions and DNA-protein interactions in fruit-specific expression of the cucumisin gene. In transient expression analysis, a chimeric gene construct containing a 1.2-kb cucumisin promoter fused to a beta-glucuronidase ...<br/><br/>Hiroshi Yamagata, Kiyoaki Yonesu, Ayako Hirata, Yasuo Aizono (2002) <i>J Biol Chem</i> <i></i> <i></i> 277: 13 11582-11590<br/>

http://publicationslist.org/yamagata/refid26 2008-11-13T05:26:19Z

The expression of asparagine synthetase (AS; EC 6.3.5.4) in response to externally supplied nitrogen was investigated with respect to enzyme activity and protein levels as detected immunologically in rice (Oryza sativa) seedlings. The asparagine content was very low in leaves and roots of nitrogen-starved rice plants but increased significantly after the supply of 1 mM NH4+ to the nutrient solutio...<br/><br/> Kawachi, Sueyoshi, Nakajima, Yamagata, Sugimoto, Oji (2002) <i>Physiol Plant</i> <i></i> <i></i> 114: 1 41-46<br/>

http://publicationslist.org/yamagata/refid16 2008-11-13T05:06:03Z

Light induced rapid and transient activation of a 46-kDa protein kinase in soybean photomixotrophic cell culture. This kinase was designated as LAP kinase (light signal-activated protein kinase). Activation of LAP kinase in response to light was associated with tyrosine phosphorylation of the kinase, and treatment of the kinase with protein tyrosine phosphatase abolished its activity. The LAP kina...<br/><br/>H Yamagata, K Saka, T Tanaka, Y Aizono (2001) <i>FEBS Lett</i> <i></i> <i></i> 494: 1-2 24-29<br/>

http://publicationslist.org/yamagata/refid7 2008-11-13T05:04:23Z

The complete nucleotide sequences of a cDNA (RSP1) that encodes a subtilisin-like serine protease (subtilase) of rice (Oryza sativa L.) and a gene (ASP48) for Arabidopsis subtilase were analyzed. The RSPI cDNA and ASP48 DNA encoded 736- and 757-residue pre-pro-polypeptides including a signal peptide with molecular masses of 78,668 Da and 79,414 Da, respectively. RSP1 is the first known serine prot...<br/><br/>H Yamagata, M Uesugi, K Saka, T Iwasaki, Y Aizono (2000) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 64: 9 1947-1957<br/>

http://publicationslist.org/yamagata/refid8 2008-11-13T05:04:23Z

A bifunctional alpha-amylase/subtilisin inhibitor (RASI) was purified to electrophoretic homogeneity from rice (Oryza sativa L.) bran. Its molecular mass was 21 kDa by SDS-PAGE and its isoelectric point was 9.05. Purified RASI inhibited subtilisin Carlsberg strongly and inhibited alpha-amylase from germinating rice seeds weakly. It inhibited rice alpha-amylase more than barley alpha-amylase, and t...<br/><br/>H Yamagata, K Kunimatsu, H Kamasaka, T Kuramoto, T Iwasaki (1998) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 62: 5 978-985<br/>

http://publicationslist.org/yamagata/refid9 2008-11-13T05:04:23Z

A cDNA encoding glutamine-dependent asparagine synthetase was isolated from dark-adapted Glycine max cell culture. The deduced amino acid sequence showed 76-89% identity with other plant sequences. The gene for asparagine synthetase is expressed predominantly in shoots as compared to roots of etiolated plants and the level of expression decreases following light treatment, suggesting that the gene...<br/><br/>H Yamagata, A Nakajima, C Bowler, T Iwasaki (1998) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 62: 1 148-150<br/>

http://publicationslist.org/yamagata/refid27 2008-11-13T05:30:00Z

From the soybean cDNA library, we isolated and analyzed the chlH gene encoding a subunit of Mg-chelatase. The subunit was a polypeptide of 1,383 amino acids with a molecular mass of 153,491 Da, which shared 90% identity with the olive gene from Antirrhinum majus. The regulation of the expression of chlH was investigated in photomix-otrophic soybean suspension cells (SB-P). The expression was light...<br/><br/>M Nakayama, T Masuda, T Bando, H Yamagata, H Ohta, K Takamiya (1998) <i>Plant Cell Physiol</i> <i></i> <i></i> 39: 3 275-284<br/>

http://publicationslist.org/yamagata/refid2 2008-11-13T04:53:28Z

The plant photoreceptor phytochrome A utilizes three signal transduction pathways, dependent upon calcium and/or cGMP, to activate genes in the light. In this report, we have studied the phytochrome A regulation of a gene that is down-regulated by light, asparagine synthetase (AS1). We show that AS1 is expressed in the dark and repressed in the light. Repression of AS1 in the light is likely contr...<br/><br/>G Neuhaus, C Bowler, K Hiratsuka, H Yamagata, N H Chua (1997) <i>EMBO J</i> <i></i> <i></i> 16: 10 2554-2564<br/>

http://publicationslist.org/yamagata/refid28 2008-11-13T05:30:00Z

A cDNA for early light-inducible protein (ELIP) was isolated from Glycine max L. and the nucleotides were sequenced. The cDNA encodes a 192-residue polypeptide of 20.3 kDa. The deduced amino acid sequence included a transit peptide in the amino-terminus and three hydrophobic regions long enough to span the thylakoid membranes, and had a high similarity to those of ELIPs and related polypeptides fr...<br/><br/>H Yamagata, C Bowler (1997) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 61: 12 2143-2144<br/>

http://publicationslist.org/yamagata/refid29 2008-11-13T05:30:00Z

Mg-insertion is the first committed step in chlorophyll synthesis and is catalyzed by Mg-chelatase. In photosynthetic bacteria, bchI gene product was suggested to be a subunit of Mg-chelatase. We isolated a bchI homolog from a soybean cDNA library and designated it as chlI. CHLI consisted of 421 amino acid residues and the sequence exhibited a high similarity to other BchI homologs. CHLI contained...<br/><br/>M Nakayama, T Masuda, N Sato, H Yamagata, C Bowler, H Ohta, Y Shioi, K Takamiya (1995) <i>Biochem Biophys Res Commun</i> <i></i> <i></i> 215: 1 422-428<br/>

http://publicationslist.org/yamagata/refid3 2008-11-13T04:53:28Z

Three signal transduction pathways, dependent on cGMP and/or calcium, are utilized by phytochrome to control the expression of genes required for chloroplast development and anthocyanin biosynthesis in plant cells. For example, chs is controlled by a cGMP-dependent pathway, cab is controlled by a calcium-dependent pathway, and fnr is regulated by a pathway that requires both cGMP and calcium. Usin...<br/><br/>C Bowler, H Yamagata, G Neuhaus, N H Chua (1994) <i>Genes Dev</i> <i></i> <i></i> 8: 18 2188-2202<br/>

http://publicationslist.org/yamagata/refid10 2008-11-13T05:04:23Z

Cucumisin is a thermostable alkaline serine protease that is found in the juice of melon fruits (Cucumis melo L.). We have determined the complete nucleotide sequence of a cucumisin cDNA (2,552 nucleotides) and deduced the corresponding amino acid sequence. The open reading frame of the cDNA consists of 731 codons and encodes a large precursor (molecular weight, 78,815) relative to the observed si...<br/><br/>H Yamagata, T Masuzawa, Y Nagaoka, T Ohnishi, T Iwasaki (1994) <i>J Biol Chem</i> <i></i> <i></i> 269: 52 32725-32731<br/>

http://publicationslist.org/yamagata/refid4 2008-11-13T04:53:28Z

We have previously used single-cell assays in a phytochrome-deficient tomato mutant to demonstrate that phytochrome signaling involves heterotrimeric G proteins, calcium, and calmodulin. While G protein activation could stimulate full chloroplast development and anthocyanin pigment biosynthesis, calcium and calmodulin could not induce anthocyanins and were only able to stimulate the development of...<br/><br/>C Bowler, G Neuhaus, H Yamagata, N H Chua (1994) <i>Cell</i> <i></i> <i></i> 77: 1 73-81<br/>

http://publicationslist.org/yamagata/refid11 2008-11-13T05:04:23Z

The complex of an adzuki bean subtilisin inhibitor (ASI-II) with its target enzyme, prepared at pH 7.6, was subjected to reversed-phase HPLC in a trifluoroacetic acid-acetonitrile system. Two peptide fragments derived from the reactive site-modified ASI-II were obtained. The analyses of the amino acid composition and sequence of these two fragments revealed that one corresponded to the region from...<br/><br/>H Nozawa, H Yamagata, Y Aizono, T Iwasaki (1992) <i>J Biochem</i> <i></i> <i></i> 111: 4 456-459<br/>

http://publicationslist.org/yamagata/refid5 2008-11-13T05:04:23Z

H Yamagata, T Nomura, S Arai, K Tanaka, T Iwasaki (1992) <i>Biosci Biotechnol Biochem</i> <i></i> <i></i> 56: 3 <br/>

http://publicationslist.org/yamagata/refid12 2008-11-13T05:04:23Z

The complete amino acid sequence of a major molecular form of subtilisin inhibitor from adzuki beans (Vigna angularis) was established by manual analysis using 4-N,N-dimethylaminoazobenzene-4'-isothiocyanate (DABITC). Sequencing was performed on the peptides which were derived by digesting the inhibitor with lysyl-endopeptidase and Staphylococcus aureus V8-protease. The inhibitor consisted of 92 a...<br/><br/>H Nozawa, H Yamagata, Y Aizono, M Yoshikawa, T Iwasaki (1989) <i>J Biochem</i> <i></i> <i></i> 106: 6 1003-1008<br/>

http://publicationslist.org/yamagata/refid30 2008-11-13T05:34:32Z

Preworkshift and postworkshift urinary fluoride concentrations were measured in 142 hydrofluoric acid (HF) workers and 82 unexposed workers aged 18 to 59. Postshift urinary fluoride concentration in HF workers was significantly higher than that in preshift or control workers. A linear relationship was observed between the mean values in urinary fluoride concentration and the HF concentration in th...<br/><br/>K Kono, Y Yoshida, H Yamagata, M Watanabe, Y Shibuya, K Doi (1987) <i>Environ Res</i> <i></i> <i></i> 42: 2 415-420<br/>

http://publicationslist.org/yamagata/refid31 2008-11-13T05:34:32Z

K Kono, Y Yoshida, H Yamagata, M Watanabe, Y Takeda, M Murao, K Doi, M Takatsu (1985) <i>Ind Health</i> <i></i> <i></i> 23: 1 37-45<br/>

http://publicationslist.org/yamagata/refid6 2008-11-13T04:58:43Z

Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the starchy endosperm protein of rice (Oryza sativa L. Japonica cv Koshihikari) during seed development confirmed that storage protein begins to accumulate about 5 days after flowering. Two polypeptide groups, 22 to 23 and 37 to 39 kilodaltons, the components of glutelin, the major storage protein in rice seed, appeared 5 days a...<br/><br/> Yamagata, Sugimoto, Tanaka, Kasai (1982) <i>Plant Physiol</i> <i></i> <i></i> 70: 4 1094-1100<br/>