ebrahim haghir ebrahimabadi

Abstract: Composition and antioxidant and antimicrobial activities of essential oil and methanol extract polar and nonpolar subfractions of Stachys inflata were determined. GC and GC/MS analyse of the essential oil showed 45 constituents representing 95.46% of the oil, the major components linalool (28.55%), α-terpineol (9.45%), spathulenol (8.37%) and (2E)-hexenal (4.62%) constituted 50.99% of it. Essential oil and extracts were also tested for their antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid assays. In the DPPH test, IC50 value for the polar subfraction was 89.50 μg/ml, indicating an antioxidant potency of about 22% of that of butylated hydroxytoluene (IC50 = 19.72 μg/ml) for this extract. In β-carotene/linoleic acid assay, the best inhibition belonged to the nonpolar subfraction (77.08%). Total phenolic content of the polar and nonpolar extract subfractions was 5.4 and 2.8% (w/w), respectively. The plant also showed a week antimicrobial activity against three strains of tested microorganisms. Linalool and α-terpineol were also tested as major components of the oil and showed no antioxidant but considerable antimicrobial activities. © 2009 Elsevier Ltd. All rights reserved.

Abstract: A series of 2-(3-chloro-2-methylphenylamino) N-substituted acetohydrazide derivatives (3a-f) have been synthesized as potential anticonvulsant agents. The 2-(3-chloro-2-methyl-phenylamino)acetamide (1) was obtained from the reaction of 3-chloro-2-methylaniline with 2-chloroacetamide. The treatment of compound 1 with hydrazine hydrate afforded 2-(3-chloro-2-methylphenylamino)acetohydrazide (2) intermediate. Reaction of compound 2 with appropriate aldehyde or ketone in absolute ethanol led to the synthesis of target compounds 3a-f. The structure of intermediates and final compounds was confirmed by elemental analysis, IR and 1H NMR spectra.