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Mario Pestarino

Dipartimento di Biologia
Università di Genova
viale Benedetto XV, 5
16132 Genova, ITALY
pesta@unige.it
Place and Date of Birth: Genova (Italy), 10.08.1947
Work Address: Dipartimento di Biologia, Università degli Studi di Genova, viale Benedetto XV 5, 16132 Genova. Tel. +39.010.3538043, fax: +39010.3533062, e-mail: pesta@unige.it
Degree in Biological Sciences: 25.03.1971, Università di Genova (Italy).
1. TEACHING ACTIVITY
1.1. Professional Career
1981-1992 Assistant professor (Researcher), Institute of Comparative Anatomy, Faculty of Sciences, University of Genova (Italy)
1983-1984 Attended to the following theoretical and practical courses "Immunolabelling for Electron Microscopy", "Immunocytochemistry for Light Microscopy", "Regulatory Peptides", "Monoclonal Antibodies", at the Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, London (UK).
1984 Attended to the practical course "Anticorpi monoclonali e loro applicazioni" at the Scuola Superiore di Oncologia e Scienze Biomediche, Istituto scientifico per lo studio e la cura dei tumori, Genova (Italy).
1991-1992 Adjunct professor of Comparative Anatomy, Facolty of Sciences, University of Genova (Italy)
1992-2000 Associate professor of Comparative Anatomy and Cytology, Faculty of Sciences, University of Genova (Italy)
2000-present Full professor of Cytology and Histology, and Cell and Developmental Biology, Faculty of Sciences, University of Genova (Italy)
2001-present Member of the Ph.D. Course in “Neurochemistry and Neurobiology” of the University of Genova (Italy)
2002-2005 Secretary-Treasurer of the Italian Society for Neuroscience
2003-present Secretary of the Course of Studies in Biotechnology, Faculty of Sciences, University of Genova (Italy)
2004-present Coordinator of the Course of High level in Molecular and cellular Biology, Faculty of Sciences, University of Genova (Italy)
1.2. Rapporteur of experimental thesis
Rapporteur of more than one hundred Thesis in Biological Sciences and Biotechnology, discussed at the University of Genova (Italy)
2. RESEARCH ACTIVITY
2.1. Collaborations with National and International Laboratories
1977-1978 Guest Investigator on “Ascidians systematics and biology”, Laboratoire de biologie des invertébrés marins et malacologie del Muséum National d'Histoire Naturelle de Paris, France, under the supervision of Prof. Claude and Françoise Monniot.
1979-1980 Guest investigator on “Ultrastructure of the branchial basket of ascidians”, Laboratoire Arago, Université Pierre et Marie Curie, Paris 6, Banyuls-sur-mer, France, under the supervision of Prof. Aline Fiala-Medioni.
1990 Guest investigator on “ Hormone peptides and growth factors on the development of ascidians and appendicularians”, Friday Harbor Laboratories, Department of Zoology, University of Washington, Seattle, USA, under the supervision of Prof. Aubrey Gorbman.
1997-1998 Guest investigator on “Histochemistry of appendicularians” Kristineberg Marine Research Station, Royal S wedish Academy of Sciences, Fiskebäckskill, Sweden, under the supervision of Prof. Ragnar Olsson.
1998-present Joint researches on “Molecular and developmental biology of the amphioxus Branchiostoma floridae” and “Sequencing of the amphioxus genome”, at the Department of Biology, University of South Florida, Tampa, FL, in collaboration of Proff. Linda and Nick Holland, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA.
1999-present Joint researches on “Role of the neurotransmitters in the development of protochordates”, in collaboration with Prof. Fiorenza De Bernardi, University of Milan, Italy.
2003-present Joint researches on “Developmental biology of the amphioxus Branchiostoma lanceolatum” in collaboration with Dr. Hector Escrivà-Garcia, Laboratoire Arago, Université Pierre et Marie Curie, Paris 6, Banyuls-sur-mer, France.
2006-present Joint researches on “Molecular evolution of the huntingtin gene” in collaboration with Prof. Elena Cattaneo, University of Milan, Italy
2006-present Joint researches on “Evolutionary biology of synapsins in protochordates” in collaboration with Prof. Fabio Benfenati, University of Genova, Italy.
Member of the following scientific societies: Unione Zoologica Italiana, Società Italiana di Istochimica, Associazione di Biologia Cellulare e del Differenziamento, Società Italiana di Neuroscienze, Ray Society of London, European Society for Comparative Endocrinologists, Society for Developmental Biology, American Association of Anatomists, The New York Academy of Sciences.
2.2. National and international research projects
1986-1992 Principal investigator of the project “"Neuroni peptidergici in vertebrati inferiori e protocordati", (MURST-40%), national coordinator Prof. Aldo Fasolo, University of Turin, Italy.
1999-2008 National coordinator of the following research projects (PRIN):
“Role of neurotransmitters in the development and differentiation of the nervous system” (MIUR-PRIN 9905111147).
“Neurotransmitters as early signals for nervous system developmentand differentiation” (MIUR-PRIN 2002055187)
“Role of neurotransmitters on neural development and differentiation: molecular, functional and evolutionary aspects” (MIUR-PRIN 2004057732).
“Transcription factors and neurotransmitters in the neural development: molecular, functional and evolutionary aspects” (MIUR-PRIN 2006058952)
2001-2004 National coordinator of the research project (FIRB) on “POU genes in the differentiation of the nervous system of protochordates” (MIUR-FIRB RBAU01WAFY).
2000-2008 Member of the international consortium on the amphioxus genome sequencing.
2.3. Referee and translation
- Referee of the following journals: Acta Biochimica et Biophysica Sinica, BMC Genomics, Brain Research, Cell and Tissue Research, European Journal of Histochemistry, European Journal of Cell Biology, Evolution and Development, Gene, The Histochemical Journal, , Marine Biology, Micron,
- Translator of the chapter “Fondamenti di Biologia molecolare”, of the book edited by Cooper G.M. and Hausman R.E., “La Cellula: un approccio molecolare”, Piccin Editore (in coll. with Dr. Simona Candiani)
2.4. Papers
Author of 76 papers in extenso published in journals with impact factor and ranked by ISI.

Books

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Journal articles

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1988
M Pestarino, J C Garaud, F Ravera (1988)  Localization of immunoreactive gastrin-like cells in the alimentary tract of the ascidian Styela plicata.   Gen Comp Endocrinol 69: 1. 13-18 Jan  
Abstract: The occurrence of a gastrin-like immunoreactivity in the alimentary tract of the ascidian Styela plicata has been investigated using immunocytochemical methods. Gastrin-like cells are present only in the gastric epithelium among the cell types responsible for digestion and absorption of food. The physiological role played by the ascidian gastrin-like peptides is discussed together with the evolutionary history of peptides of the gastrin/cholecystokinin family.
Notes:
M Pestarino (1988)  Occurrence of melanotropin- and corticotropin-like material in the cerebral ganglion of a protochordate.   Neurosci Lett 87: 3. 210-214 May  
Abstract: The cerebral ganglion of the ascidian Styela plicata has been investigated using cytochemical and immunocytochemical methods. Peptidergic neurons are present mainly in the cortical zone and they contain alpha-MSH or ACTH-like immunoreactive substances. Coexistence of the two pituitary hormones is also demonstrated in some neurons by means of the double immunofluorescence method. The possible physiological role of these peptides in the ascidian central nervous system is discussed as well as the conservative characteristics of alpha-MSH- and ACTH-like molecules.
Notes:
1985
M Pestarino (1985)  CCK-like peptides in the neural complex of a protochordate.   Peptides 6 Suppl 3: 389-392  
Abstract: The neural complex of the ascidian Styela plicata has been investigated by means of cytochemical and immunocytochemical methods. In the cerebral ganglion, using a mammalian antibody to synthetic CCK-8, immunoreactive neurons and nerve fibers have been localized; at the same time immunofluorescent cells are scattered in some glandular lobules of the neural gland. The possible functions of a CCK-8-like peptide in ascidians is suggested and discussed.
Notes:
M Pestarino (1985)  A pituitary-like role of the neural gland of an ascidian.   Gen Comp Endocrinol 60: 2. 293-297 Nov  
Abstract: The neural gland of the ascidian Styela plicata has been investigated by means of the indirect immunofluorescence method. The possible homology with the vertebrate anterior pituitary is raised and discussed with reference to the existing literature and to the presence of ACTH-like immunoreactive cells in the neural gland.
Notes:
1984
M Pestarino (1984)  Immunocytochemical demonstration of prolactin-like activity in the neural gland of the ascidian Styela plicata.   Gen Comp Endocrinol 54: 3. 444-449 Jun  
Abstract: The neural gland of the ascidian Styela plicata has been studied by means of cytochemical and immunocytochemical methods. Several cells of the glandular lobules are erythrosin-positive and immunoreactive after incubation with a mammalian antibody to human prolactin. On the basis of the results obtained and of the existing literature, the possible pituitary-like function of the neural gland is discussed.
Notes:
1983
M Pestarino (1983)  Immunofluorescent localisation of somatostatin-like cells in the esophagus of a protochordate.   Arch Anat Microsc Morphol Exp 72: 4. 273-278  
Abstract: By means of the indirect immunofluorescence method, somatostatin-like immunoreactive (SLI) cells are detected in the esophagus of the ascidian Styela plicata. They are of the "open" type; they act probably by a paracrine mechanism on the esophageal secretin-like cells. The ascidian SLI cells are negative to all the specific cytochemical methods characteristic of vertebrate somatostatin (D) cells, which were applied in this work. In consequence, special SLI cells are probably present in ascidians.
Notes:
M Pestarino, G Tagliafierro (1983)  Immunocytochemical localization of secretin-like cells in the digestive tract of the ascidian Halocynthia papillosa.   Basic Appl Histochem 27: 2. 141-148  
Abstract: The digestive tract of the ascidian Halocynthia papillosa was studied by means of both immunofluorescent and immunocytochemical methods. In the esophageal epithelium there are immunoreactive secretin-like (S2) cells, which are at the same time serotonin-containing cells. In the gastric and intestinal epithelia, immunoreactive (S1) cells are present which contain exclusively secretin-like substances. On the basis of the comparison between the gastric morphology of H. papillosa and that of other ascidians (styelids) it is possible to reach some conclusions of an evolutionary nature.
Notes:
M Pestarino (1983)  Prolactinergic neurons in a protochordate.   Cell Tissue Res 233: 2. 471-474  
Abstract: Peptidergic neurons have been detected in the cerebral ganglion of the ascidian Styela plicata by means of cytochemical methods. After incubation with a mammalian antibody to human prolactin the perikarya show a strong immunoreactivity. The possible function of prolactin-like peptides in the nervous system of protochordates is discussed.
Notes:
1982
M Pestarino (1982)  Cytochemical investigations on endocrine-like cells of the digestive tract of the ascidian Styela plicata.   Basic Appl Histochem 26: 2. 107-116  
Abstract: The digestive tract of Styela plicata was examined by cytochemical methods with the aim of displaying the endocrine-like cells. There are different endocrine-like cell types in the gastric epithelium which can be distinguished by their cytochemical characteristics in G-like (Type I), A(A2)-like (Type II), B-like (Type III) and (D (A1)-like (Type IV) cells; while in the esophageal and intestinal epithelium only enterochromaffin-like cells are present.
Notes:
M Pestarino (1982)  Occurrence of different secretin-like cells in the digestive tract of the ascidian Styela plicata (Urochordata, Ascidiacea).   Cell Tissue Res 226: 1. 231-235  
Abstract: Secretin-like cells have been detected in the digestive tract of the ascidian Styela plicata by means of immunofluorescent and immunocytochemical methods. Especially, in the esophageal epithelium there are immunoreactive cells (S2) in which a biogenic amine (5-HT) and a regulatory peptide (secretin) occur together. In the gastric epithelium only secretin-like cells (S1) are present. Tests of cross-reactivity performed with glucagon, GIP and VIP, have confirmed the presence of a secretin-like molecule only in the S1 and S2 cells.
Notes:
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1971

Conference papers

2004
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2002
R PENNATI, S GROPPELLI, U SOTGIA, M FASCIO, M PESTARINO, DE BERNARDI F (2002)  Serotonin localization in Phallusia mammillata larvae and during first events of metamorphosis    
Abstract:
Notes: Gli atti sono stati pubblicati sulla rivista Animal Biology, 11: 3-14, 2002
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