Abstract: Apolipoprotein (Apo E) gene polymorphism was determined in 53 unrelated Bangladeshi individual by using restriction enzyme isoform genotyping. This gene is polymorphic, with three common alleles coding for three major isoforms ε2, ε3 and ε4, resulting in six genotypes (ε2/ε2, ε3/ε2, ε4/ε2, ε3/ε3, ε4/ε3 and ε4/ε4). These isoforms differ from each other by a single amino acid substitution. Apo E functions as ligands of various receptors and determines the metabolic fate of lipoproteins. The genetic variations at the Apo E locus could thus be a major determinant of the inter-individual variation in susceptibility to coronary artery disease. The allele frequencies for Apo ε2, ε3 and ε4 alleles were found to be 0.05, 0.80 and 0.15, respectively in Bangladeshi population. The allele frequencies found in this study were compared with other Asian populations using Chi-square (χ2) test and cluster analysis. The Bangladeshi population shows close relationship with Japanese population living in Tokyo, Sendai and Indian populations living in Singapore. This study found ε3 to be the predominant isoform but the relative proportions of the three isoforms have shown variation among populations.