Dr Pavlos Sarafis has graduated from the School of Nursing Officers and served in the Greek Navy. He holds M.Sc. degrees in Management of Health Services, in Public Health, and in Management of Mass Destruction and Emergency Situations. He completed his PhD degree in Basic Research/Surgical Infections at the University of Athens, School of Medicine, with a scholarship from Alexander Onassis Public Benefit Foundation. He specialized in Travel Medicine at the Ludwig-Maximilians-Universität of Munich, where he assisted in the H5N1 vaccine production research (clinical phases I/II), as a postdoctoral student. He has delivered courses in Surgical Nursing, Travel Medicine, and Intercultural Nursing at the University of Peloponnese, Faculty of Nursing. He teaches Travel Medicine and Infections to undergraduate and postgraduate students at the University of Athens, Faculty of Nursing, as a research associate. He is part of the collaborating teaching staff of the Hellenic Open University, at the postgraduate programs of Health Care Management and National Health System Services Management. He participates in scientific committees of Greek and international journals. He has a wide range of publication and research activities, and he has participated in the writing process of books related to his professional and scientific interests. Since October 2010, he holds the position of Lecturer at the Technological Educational Institute of Lamia, Faculty of Nursing.
Abstract: Human papillomavirus (HPV) infection is a causative factor for cervical cancer. Early detection of high risk HPV types might help to identify women at high risk of cervical cancer. The aim of the present study was to examine the HPV prevalence and distribution in cervical smears in a sample of Greek women attending a gynecological outpatient clinic and to explore the determinants of the infection.
Abstract: Patients who are treated in an intensive care unit (ICU) show the need of recovery of their renal function. The reason is that, in this particular cohort of patients, we have to maintain the necessary balance between body fluids, electrolytes, and acid-base, try to suspend further renal damage and purify the patient's blood to better accept the given therapy. In this paper, we try to demonstrate all the methods that can be used depending on the patient's condition, the therapist's preferences and the hospital's capabilities.
Abstract: In vitro combinations of beta-lactams with fluoroquinolones against multidrug-resistant (MDR) Pseudomonas aeruginosa were tested. From a total of 200 isolates, 24 genetically distinct isolates defined by pulsed-field gel electrophoresis (PFGE) were selected. The isolates were exposed over time to imipenem, meropenem and ceftazidime as well as to their combinations with ciprofloxacin and moxifloxacin. All isolates were resistant to all agents tested at concentrations equal to their average serum level. Synergy of any of the tested combinations was found in 10 isolates (41.7%). This was shown after 4h and 6h of exposure accompanied by re-growth after 24h. Not all the tested combinations were active against the same isolates. The combinations of imipenem+ciprofloxacin, ceftazidime+ciprofloxacin and imipenem+moxifloxacin were the most active. When time-kill assays were repeated for the latter isolates at antimicrobial concentrations equal to their maximum serum levels, synergy was prolonged to 24h. The present findings should be interpreted with caution for the management of infections by MDR P. aeruginosa. They underscore the potential interest of reporting synergism between beta-lactams and fluoroquinolones in the nosocomial setting when a MDR isolate emerges.
