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{userid:"alltoy01", "articletype":"article","pages":"596-605","author":"Todd Ashworth, Ananda L Roy","year":"2009","title":"Phase specific functions of the transcription factor TFII-I during cell cycle.","month":"Feb","journal":"Cell Cycle","publisher":"","volume":"8","number":"4","note":"","tags":"Animals,CDC2 Protein Kinase,Cell Cycle,Chromatin,Gene Expression Profiling,Gene Silencing,Hela Cells,Humans,Mice,Oligonucleotide Array Sequence Analysis,Transcription Factors, TFII","booktitle":"","editor":"","abstract":"The post-embryonic cells, in a non-proliferating quiescent state (G(0)), require mitogenic signaling to drive them into cell cycle entry (G(1)). However, cell cycle events become largely independent of external signaling once cells begin DNA synthesis in S phase. Given these two phases of cell cycle are mechanistically distinct, it is unclear whether there could be coordinated transcriptional regulation between these phases. The signal induced multifunctional transcription factor TFII-I, upon growth factor signaling, undergoes tyrosine phosphorylation, which is essential for its transcriptional activation function and corresponding G(0)-G(1) transition. Here we show that silencing of TFII-I has unexpected defects in S-phase. The TFII-I KD cells exhibit significant delay entering into and executing S-phase progression and entry into G(2)\/M phase but do not exhibit any significant mitotic defects as evidenced by post-mitotic G(1) entry and frequency of binucleation. Microarray analysis, coupled with functional validation, reveals cyclin D1 and PKC-beta as major downstream targets of TFII-I. Cyclin D1 is induced in G(1) and is necessary for G(1)\/S transition. PKC-beta also activates cyclin D1 via NFkappaB. These observations suggest a transcriptional network during early phases of cell cycle mediated by TFII-I. Finally, we show that Cdk1 phosphorylates TFII-I at the G(2)\/M boundary, which likely leads to its displacement from the condensed chromatin during prophase to pro-metaphase transition. Taken together, TFII-I appears to have distinct roles in distinct phases of the mammalian cell cycle.","address":"","school":"","issn":"1551-4005","doi":"","isi":"","pubmed":"19182516","key":"Ashworth2009","howpublished":"","urllink":"","refid":3}
,

{userid:"alltoy01", "articletype":"article","pages":"2631-2635","author":"Todd Ashworth, Ananda L Roy","year":"2007","title":"Cutting Edge: TFII-I controls B cell proliferation via regulating NF-kappaB.","month":"Mar","journal":"J Immunol","publisher":"","volume":"178","number":"5","note":"","tags":"Active Transport, Cell Nucleus,Animals,Apoptosis,B-Lymphocytes,Cell Line,Cell Nucleus,Cell Proliferation,Down-Regulation,Immunoglobulin M,Mice,NF-kappa B p50 Subunit,Proto-Oncogene Proteins c-rel,Signal Transduction,Transcription Factors, TFII,Transforming Growth Factor beta,Up-Regulation","booktitle":"","editor":"","abstract":"The multifunctional transcription factor TFII-I physically and functionally interacts with Bruton's tyrosine kinase in murine B cells. However, the downstream functions of TFII-I in B cells are unknown. Toward achieving this goal, we established stable posttranscriptional silencing of TFII-I in WEHI-231 immature murine B cells, which undergoes growth arrest and apoptosis either upon anti-IgM or TGF-beta signaling. In this study, we show that TFII-I promotes growth arrest of cells in a signal-dependent manner. Unlike control cells, B cells exhibiting loss of TFII-I function fail to undergo arrest upon signaling due to up-regulation of c-Myc expression and concomitant down-regulation of both p21 and p27. Loss of TFII-I is also associated with simultaneous increase in nuclear c-rel and decrease in p50 homodimer binding. Thus, besides controlling c-myc transcription, TFII-I controls B cell proliferation by regulating both nuclear translocation of c-rel and DNA-binding activity of p50 NF-kappaB.","address":"","school":"","issn":"0022-1767","doi":"","isi":"","pubmed":"17312101","key":"Ashworth2007","howpublished":"","urllink":"","refid":8}
,

{userid:"jill.maben", "articletype":"article","pages":"e36-e48","author":"P Griffiths, T Murrells, J Maben, S Jones, M Ashworth","year":"2010","title":"Nurse staffing and quality of care in UK general practice : cross-sectional study using routinely collected data","month":"","journal":"British Journal of General Practice","publisher":"","volume":"60","number":"1","note":"[1] xD;[2] xD;[3]","tags":"","booktitle":"","editor":"","abstract":"","address":"","school":"","issn":"","doi":"","isi":"","pubmed":"","key":"Griffiths2010a","howpublished":"http:\/\/www.ingentaconnect.com\/content\/rcgp\/bjgp\/2010\/00000060\/00000570\/art00009","urllink":"http:\/\/www.ingentaconnect.com\/content\/rcgp\/bjgp\/2010\/00000060\/00000570\/art00009","refid":152}
,

{userid:"jill.maben", "refid":"157","repocollections":"","attachment":"","_thumb":"","articletype":"inproceedings","sectionheading":"","title":"Nurse Staffing and Quality of Care in UK General Practice : Cross sectional study using routinely collected data. (poster). 1st - 3rd June 2009.","year":"2009","author":"P Griffiths, T Murrells, J Maben, S Jones, M Ashworth","booktitle":"Delivering Better Health Services: Annual NHS Confederation SDO \/ Health Service Research Network Conference. Birmingham.","editor":"","pages":"","organization":"","address":"","publisher":"","doi":"","pubmed":"","pdflink":"","urllink":"","abstract":"","note":"","tags":"","month":"","journal":"","volume":"","number":"","school":"","issn":"","isi":"","key":"Griffiths2009","howpublished":""}
,

{userid:"j.f.campodonico", "refid":9,"repocollections":"","attachment":"","_thumb":"","articletype":"article","sectionheading":"","title":"Mapuchea pinoi sp. nov. (Hemiptera: Membracoidea: Myerslopiidae): especie f\u00f3sil de un lecho de turba de Monteverde, regi\u00f3n de Los Lagos, Chile.","year":"2017","author":"J F Campodonico, E I Fa\u00fandez, A C Ashworth","journal":"Anales del Instituto de la Patagonia","volume":"45","number":"1","pages":"49-52","month":"","doi":"10.4067\/S0718-686X2017000100049","pubmed":"","pdflink":"http:\/\/www.scielo.cl\/pdf\/ainpat\/v45n1\/0718-686X-ainpat-45-01-00049.pdf","urllink":"","abstract":"Se describe Mapuchea pinoi sp. nov. (Myerslopiidae) a partir de material f\u00f3sil de Monte Verde, a orillas de la cala de Chinchihuapi, regi\u00f3n de Los Lagos, Chile, en base a un tegmen izquierdo, con una data de 13.429 a\u00f1os AP. A pesar de ser una especie descrita en base a material f\u00f3sil, se cree que esta especie podr\u00eda no encontrarse extinta.\r\n\r\nA fossil myerslopiid, Mapuchea pinoi sp. nov., is described from Monte Verde, Los Lagos Region, Chile. The new species is described on basis of a left tegmen, of 13,429 yr. BP. Although it is a fossil species it is possible that this taxon is not extinct.","note":"","tags":"Myerslopiidae, Mapuchea, nueva especie, Chile, Monte Verde, f\u00f3sil","weight":9}
,

{userid:"jill.maben", "refid":"156","repocollections":"","attachment":"","_thumb":"","articletype":"inproceedings","sectionheading":"","title":"Nurse staffing and quality of care in UK general practice : cross-sectional study using routinely collected data. 12-15 May 2010.","year":"2010","author":"P Griffiths, T Murrells, J Maben, S Jones, M Ashworth, D Dawoud","booktitle":"Royal College of Nursing International Research Conference. Gateshead.","editor":"","pages":"","organization":"","address":"","publisher":"","doi":"doi:10.3399\/bjgp10X482086","pubmed":"","pdflink":"","urllink":"","abstract":"","note":"","tags":"","month":"","journal":"","volume":"","number":"","school":"","issn":"","isi":"","key":"Griffiths2010","howpublished":""}
,

{userid:"antonywoliver", "articletype":"article","pages":"990-996","author":"Antony W Oliver, Sally Swift, Christopher J Lord, Alan Ashworth, Laurence H Pearl","year":"2009","title":"Structural basis for recruitment of BRCA2 by PALB2.","month":"Sep","journal":"EMBO reports","publisher":"","volume":"10","number":"9","note":"","tags":"Amino Acid Sequence,Animals,Apoptosis Regulatory Proteins,BRCA2 Protein,Binding Sites,Cell Line,Crystallography, X-Ray,Fanconi Anemia Complementation Group N Protein,Humans,Models, Molecular,Molecular Sequence Data,Nuclear Proteins,Protein Binding,Protein Structure, Quaternary,Protein Structure, Tertiary,Sequence Alignment,Tumor Suppressor Proteins","booktitle":"","editor":"","abstract":"The breast cancer 2, early onset protein (BRCA2) is central to the repair of DNA damage by homologous recombination. BRCA2 recruits the recombinase RAD51 to sites of damage, regulates its assembly into nucleoprotein filaments and thereby promotes homologous recombination. Localization of BRCA2 to nuclear foci requires its association with the partner and localizer of BRCA2 (PALB2), mutations in which are associated with cancer predisposition, as well as subtype N of Fanconi anaemia. We have determined the structure of the PALB2 carboxy-terminal beta-propeller domain in complex with a BRCA2 peptide. The structure shows the molecular determinants of this important protein-protein interaction and explains the effects of both cancer-associated truncating mutants in PALB2 and missense mutations in the amino-terminal region of BRCA2.","address":"","school":"","issn":"1469-3178","doi":"10.1038\/embor.2009.126","isi":"","pubmed":"19609323","key":"Oliver2009","howpublished":"","urllink":"","refid":28,"weight":28}
,

{userid:"michelle.power", "articletype":"article","pages":"190-196","author":"Elke T Vermeulen, Deborah L Ashworth, Mark D B Eldridge, Michelle L Power","year":"2015","title":"Diversity of Cryptosporidium in brush-tailed rock-wallabies (Petrogale penicillata) managed within a species recovery programme.","month":"Aug","journal":"International journal for parasitology. Parasites and wildlife","publisher":"","volume":"4","number":"2","note":"","tags":"","booktitle":"","editor":"","abstract":"Host-parasite relationships are likely to be impacted by conservation management practices, potentially increasing the susceptibility of wildlife to emerging disease. Cryptosporidium, a parasitic protozoan genus comprising host-adapted and host-specific species, was used as an indicator of parasite movement between populations of a threatened marsupial, the brush-tailed rock-wallaby (Petrogale penicillata). PCR screening of faecal samples (n\u2009=\u2009324) from seven wallaby populations across New South Wales, identified Cryptosporidium in 7.1% of samples. The sampled populations were characterised as captive, supplemented and wild populations. No significant difference was found in Cryptosporidium detection between each of the three population categories. The positive samples, detected using 18S rRNA screening, were amplified using the actin and gp60 loci. Multi-locus sequence analysis revealed the presence of Cryptosporidium fayeri, a marsupial-specific species, and C.\u2009meleagridis, which has a broad host range, in samples from the three population categories. Cryptosporidium meleagridis has not been previously reported in marsupials and hence the pathogenicity of this species to brush-tailed rock-wallabies is unknown. Based on these findings, we recommend further study into Cryptosporidium in animals undergoing conservation management, as well as surveying wild animals in release areas, to further understand the diversity and epidemiology of this parasite in threatened wildlife.","address":"","school":"","issn":"2213-2244","doi":"10.1016\/j.ijppaw.2015.02.005","isi":"","pubmed":"25834789","key":"Vermeulen2015","howpublished":"","urllink":"","refid":40,"weight":40}
,

{userid:"michelle.power", "articletype":"article","pages":"277-280","author":"Elke T Vermeulen, Deborah L Ashworth, Mark D B Eldridge, Michelle L Power","year":"2015","title":"Investigation into potential transmission sources of Giardia duodenalis in a threatened marsupial (Petrogale penicillata).","month":"Jul","journal":"Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases","publisher":"","volume":"33","number":"","note":"","tags":"Animal Diseases,Animals,Giardia lamblia,Giardiasis,Macropodidae,Phylogeny,Protozoan Proteins,RNA, Ribosomal, 18S,Sequence Analysis, DNA","booktitle":"","editor":"","abstract":"Assemblages of the protozoan parasite Giardia duodenalis common in humans and domestic species are increasingly identified in wildlife species, raising concern about the spill-over of pathogens from humans and domestic animals into wildlife. Here, the identity and prevalence of G. duodenalis in populations of a threatened marsupial, the brush-tailed rock-wallaby (Petrogale penicillata), was investigated. Identification of G. duodenalis isolates, across three loci (18S rRNA, \u03b2-giardin and gdh), from rock-wallaby fecal samples (n = 318) identified an overall detection rate of 6.3%. No significant difference in G. duodenalis detection was found among captive, wild and supplemented populations. Isolates were assigned to the zoonotic assemblages A and B at 18S rRNA, with sub-assemblages AI and BIV identified at the \u03b2-giardin and gdh loci, respectively. Assemblages AI and BIV have previously been identified in human clinical cases, but also in domestic animals and wildlife. The identification of these assemblages in brush-tailed rock-wallabies suggests there are transmission routes of G. duodenalis from humans or other animals to Australian wildlife, both in captivity and in the wild.","address":"","school":"","issn":"1567-7257","doi":"10.1016\/j.meegid.2015.05.015","isi":"","pubmed":"25986646","key":"Vermeulen2015","howpublished":"","urllink":"","refid":"45","weight":"45"}
,

{userid:"alltoy01", "articletype":"article","pages":"301-308","author":"Shweta Hakre, Mar\u00eda Isabel Tussie-Luna, Todd Ashworth, Carl D Novina, Jeffrey Settleman, Phillip A Sharp, Ananda L Roy","year":"2006","title":"Opposing functions of TFII-I spliced isoforms in growth factor-induced gene expression.","month":"Oct","journal":"Mol Cell","publisher":"","volume":"24","number":"2","note":"","tags":"3T3 Cells,Alternative Splicing,Animals,COS Cells,Cell Nucleus,Cercopithecus aethiops,Fibroblasts,Gene Expression Regulation,Intercellular Signaling Peptides and Proteins,Mice,Protein Isoforms,Protein Structure, Tertiary,Proto-Oncogene Proteins c-fos,Signal Transduction,Transcription Factors, TFII","booktitle":"","editor":"","abstract":"Multifunctional transcription factor TFII-I has two spliced isoforms (Delta and beta) in murine fibroblasts. Here we show that these isoforms have distinct subcellular localization and mutually exclusive transcription functions in the context of growth factor signaling. In the absence of signaling, TFII-Ibeta is nuclear and recruited to the c-fos promoter in vivo. But upon growth factor stimulation, the promoter recruitment is abolished and it is exported out of the nucleus. Moreover, isoform-specific silencing of TFII-Ibeta results in transcriptional activation of the c-fos gene. In contrast, TFII-IDelta is largely cytoplasmic in the resting state but translocates to the nucleus upon growth factor signaling, undergoes signal-induced recruitment to the same site on the c-fos promoter, and activates the gene. Importantly, activated TFII-IDelta interacts with Erk1\/2 (MAPK) kinase in the cell cytoplasm and imports the Erk1\/2 to the nucleus, thereby transducing growth factor signaling. Our results identify a unique growth factor signaling pathway controlled by opposing activities of two TFII-I spliced isoforms.","address":"","school":"","issn":"1097-2765","doi":"10.1016\/j.molcel.2006.09.005","isi":"","pubmed":"17052463","key":"Hakre2006","howpublished":"","urllink":"","refid":10}
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