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{userid:"yha111", "articletype":"article","pages":"719-730","author":"S Stefanos, Y H Ahn, S Pestka","year":"1989","title":"Characterization of human interferon-gamma receptor purified from placenta.","month":"Dec","journal":"Journal of interferon research","publisher":"","volume":"9","number":"6","note":"","tags":"Cross-Linking Reagents,Female,Humans,Interferon-gamma,Kinetics,Molecular Weight,Placenta,Pregnancy,Receptors, Immunologic,Receptors, Interferon","booktitle":"","editor":"","abstract":"Membranes prepared from human placenta were used for characterization of the receptor for human interferon-gamma (HuIFN-gamma) after large-scale purification. HuIFN-gamma linked covalently to Affigel-10 was used for the purification of the receptor from octylglucoside-solubilized placental membranes. Radiolabeled IFN-gamma [32P]HuIFN-gamma, was used in binding and cross-linking studies to detect the receptor at different stages of the purification. From binding assays it was calculated that an average placenta contained 90-120 micrograms of receptor with a Kd value of 1.3 x 10(-9) M. Thus, human placenta is a rich and convenient source of receptor for IFN- gamma. When purified receptor was cross-linked to [32P]HuIFN-gamma, a variety of cross-linked bands were detected dependent on the preparation conditions. The use of protease inhibitors in the course of processing prevented degradation of the 90-kD intact receptor, showing that the lower-molecular-weight products detected in previous studies are degradation products of the receptor. Furthermore, a 20-kD fragment of the receptor was found to be active in binding HuIFN-gamma.","address":"","school":"","issn":"0197-8357","doi":"","isi":"","pubmed":"2532663","key":"Stefanos1989","howpublished":"","urllink":"","refid":51,"weight":51}
,

{userid:"yha111", "articletype":"article","pages":"83-90","author":"J W Kim, Y H Ahn","year":"1998","title":"CCAAT\/enhancer binding protein regulates the promoter activity of the rat GLUT2 glucose transporter gene in liver cells.","month":"Nov","journal":"Biochem J","publisher":"","volume":"336 ( Pt 1)","number":"","note":"","tags":"Animals,Base Sequence,CCAAT-Enhancer-Binding Proteins,Chloramphenicol O-Acetyltransferase,DNA Primers,DNA-Binding Proteins,Gene Expression Regulation,Glucose Transporter Type 2,Humans,Liver,Male,Monosaccharide Transport Proteins,Nuclear Proteins,Promoter Regions, Genetic,Protein Binding,Rats,Rats, Sprague-Dawley,Transcription Factors,Tumor Cells, Cultured","booktitle":"","editor":"","abstract":"The liver-specific expression of the GLUT2 glucose transporter gene is suppressed in cultured hepatoma cell lines as well as in hepatocytes in primary culture. To understand the underlying mechanism involved in this process, we analysed the rat GLUT2 promoter region. A DNase I footprinting assay with rat liver nuclear extract revealed eight protected regions within a -500 bp region of the GLUT2 promoter (sites A to H). Three of these sites (B, F and H) were occupied by transcription factors that are considerably enriched in liver cells compared with spleen or kidney. The proteins binding to these sites were investigated by a combination of DNase I footprinting assay and electrophoretic mobility-shift assay with the addition of specific oligonucleotide competitors and specific antibody against known transcription factors. As a result it was revealed that hepatocyte nuclear factor 3 binds to site B (-120 to -70), and CCAAT\/enhancer binding protein alpha (C\/EBPalpha) and C\/EBPbeta bind to site F (-375 to -356) and site H (-500 to -471). The binding of C\/EBP to sites F and H was markedly decreased within 4 h when liver cells were subjected to primary culture, suggesting that C\/EBP might be responsible for the decreased expression of GLUT2 in this process. In contrast, Western blot analysis revealed that C\/EBPalpha began to decrease after 1 h of hepatocyte culture, and C\/EBPbeta was not changed significantly throughout the culture period, suggesting that C\/EBP could be regulated at the transcriptional level as well as the post-translational level when hepatocytes were put in culture. To confirm the role of C\/EBP in the regulation of GLUT2 promoter activity, sites F and H were ligated to a chloramphenicol acetyltransferase (CAT) reporter gene and co-transfected with a C\/EBP expression vector into HepG2 cells. The co-expression of C\/EBPalpha and C\/EBPbeta resulted in 9.1-fold and 3. 8-fold increases of CAT activities in the site F-CAT and site H-CAT constructs respectively. These results indicate that C\/EBPalpha and C\/EBPbeta regulate the promoter activity of the GLUT2 gene and might be responsible for the down-regulation of the GLUT2 gene when hepatocytes are subjected to primary culture.","address":"","school":"","issn":"0264-6021","doi":"","isi":"","pubmed":"9806888","key":"Kim1998","howpublished":"","urllink":"","refid":28}
,

{userid:"Ahn_SG", "articletype":"article","pages":"","author":"S-G Ahn, S-J Tahk, J-H Shin","year":"2006","title":"Images in cardiology. Abnormal left atrial membranous structure in transthoracic echocardiography caused by external compression from a large bronchogenic cyst.","month":"Feb","journal":"Heart","publisher":"","volume":"92","number":"2","note":"","tags":"Adult,Bronchogenic Cyst,Cardiomyopathies,Dyspnea,Echocardiography,Female,Heart Atria,Humans","booktitle":"","editor":"","abstract":"","address":"","school":"","issn":"1468-201X","doi":"10.1136\/hrt.2005.068171","isi":"","pubmed":"16415191","key":"Ahn2006","howpublished":"","urllink":"","refid":21}
,

{userid:"yha111", "articletype":"article","pages":"15-20","author":"J W Kim, Y K Kim, Y H Ahn","year":"1998","title":"A mechanism of differential expression of GLUT2 in hepatocyte and pancreatic beta-cell line.","month":"Mar","journal":"Exp Mol Med","publisher":"","volume":"30","number":"1","note":"","tags":"Animals,Binding Sites,Cell Line,DNA Footprinting,Deoxyribonuclease I,Gene Expression Regulation,Glucose Transporter Type 2,Islets of Langerhans,Liver,Monosaccharide Transport Proteins,Promoter Regions, Genetic,Protein Binding,Rats,Transcription Factor AP-1","booktitle":"","editor":"","abstract":"DNase I footprinting assay using liver nuclear extracts revealed six protected regions between nucleotide -600 and +110 and hence named Box I-VI. Upstream promoter element (UPE), a DNA element playing crucial role in transcriptional control of the tissue specific expression of pancreatic beta-cell, has been detected within the proximal region of rat GLUT2 promoter. This region is included in Box VI. The protein-DNA interaction in this region (Box VI) was confirmed by mobility shift assay using liver nuclear extracts. Deletion of the region between -585 bp and -146 bp resulted in dramatic changes in promoter activity when they were expressed in liver and beta-cell derived cell line. When -585\/-146 construct was expressed in liver, the activity was decreased to 46%, whereas the activity in beta-cell line, HIT-T15 cell, was increased by 84% when compared to -146\/+190 construct. These opposing phenomena can be explained by the fact that beta-cell specifically expresses the UPE binding protein. Assuming that there may be Box VI-binding protein playing negative roles both in hepatocyte and beta-cell, and that the protein acts as a negative regulator of GLUT2 gene, the UPE binding protein in the beta-cell may overcome the inhibition by binding to the protein.","address":"","school":"","issn":"1226-3613","doi":"","isi":"","pubmed":"9873817","key":"Kim1998","howpublished":"","urllink":"","refid":27}
,

{userid:"jeongseoky", "articletype":"article","pages":"226-227","author":"J J Yang, S Y Cho, H.-J. Ahn, H J Lee, W.-I. Lee, T S Park","year":"2014","title":"Mean platelet volume in acute appendicitis : A gender difference","month":"","journal":"Platelets","publisher":"","volume":"25","number":"3","note":"","tags":"","booktitle":"","editor":"","abstract":"","address":"","school":"","issn":"","doi":"","isi":"","pubmed":"","key":"Yang2014d","howpublished":"","urllink":"","refid":78,"weight":78}
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{userid:"dorian.arnold", "articletype":"inproceedings","pages":"1115-1122","author":"Joshua D Goehner, Taylor L Groves, Dorian C Arnold, Dong H Ahn, Gregory L Lee","year":"2013","title":"An Optimal Algorithm for Extreme Scale Job Launching","month":"","journal":"","publisher":"","volume":"","number":"","note":"Appeared in The 11th IEEE International Symposium on Parallel and Distributed Processing with Applications (ISPA-13), July 16-18, 2013, Melbourne, Australia. Published in the described proceedings.","tags":"SSL, System Services, Job Launch","booktitle":"12th IEEE International Conference on Trust, Security and Privacy in Computing and Communications (TrustCom)","editor":" ","abstract":"","address":"Melbourne, Australia","school":"","issn":"","doi":"","isi":"","pubmed":"","key":"Goehner2013Optimal","howpublished":"","urllink":"","refid":170,"weight":170}
,

{userid:"dorian.arnold", "articletype":"inproceedings","pages":"578-585","author":"Dong H Ahn, Dorian C Arnold, B Supinski, Gregory L Lee, Barton P Miller, Martin Schulz","year":"2008","title":"Overcoming Scalability Challenges for Tool Daemon Launching","month":"September","journal":"","publisher":"","volume":"","number":"","note":"Acceptance rate: 81\/263: 30.8%.","tags":"Middleware, MRNet, HPC Tools, Job launching","booktitle":"37th International Conference on Parallel Processing (ICPP-08)","editor":" ","abstract":"","address":"Portland, OR, USA","school":"","issn":"","doi":"","isi":"","pubmed":"","key":"Ahn2008Overcoming","howpublished":"","urllink":"","refid":198,"weight":198}
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{userid:"taylorlgroves", "refid":"3","repocollections":"","attachment":"","_thumb":"","articletype":"inproceedings","sectionheading":"","title":"An Optimal Algorithm for Extreme Scale Job Launching","year":"2013","author":"Joshua D Goehner, Taylor L Groves, Dorian C Arnold, Dong H Ahn, Gregory L Lee","booktitle":"Trust, Security and Privacy in Computing and Communications (TrustCom), 2013 12th IEEE International Conference on","editor":" ","pages":"1115-1122","organization":"","address":"","publisher":"","doi":"","pubmed":"","pdflink":"http:\/\/taylorgroves.com\/mwiki\/images\/9\/92\/ISPA-140.pdf","urllink":"http:\/\/ieeexplore.ieee.org\/xpls\/abs_all.jsp?arnumber=6680956","abstract":"","note":"","tags":"SSL, middleware, DOE","weight":3,"month":"","journal":"","volume":"","number":"","school":"","issn":"","isi":"","key":"goehner2013optimal","howpublished":""}
,

{userid:"karapanagioti", "articletype":"article","pages":"6516-6526","author":"S Ahn, D Werner, H K Karapanagioti, D R McGlothlin, R N Zare, R G Luthy","year":"2005","title":"Phenanthrene and pyrene sorption and intraparticle diffusion in polyoxymethylene, coke, and activated carbon","month":"SEP 1","journal":"ENVIRONMENTAL SCIENCE & TECHNOLOGY","publisher":"","volume":"39","number":"17","note":"","tags":"","booktitle":"","editor":"","abstract":"","address":"","school":"","issn":"0013-936X","doi":"10.1021\/es050113o","isi":"WOS:000231723800035","pubmed":"","key":"Ahn2005","howpublished":"","urllink":"","refid":92,"weight":92}
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{userid:"dorian.arnold", "articletype":"inproceedings","pages":"621-628","author":"Gregory L Lee, Dong H Ahn, Dorian C Arnold, Bronis R De Supinski, Barton P Miller, Martin Schulz","year":"2007","title":"Benchmarking the Stack Trace Analysis Tool for BlueGene\/L","month":"September","journal":"","publisher":"","volume":"","number":"","note":"","tags":"MRNet, Middleware, STAT, HPC Tools","booktitle":"Parallel Computing 2007","editor":"","abstract":"","address":"","school":"","issn":"","doi":"","isi":"","pubmed":"","key":"Lee2007Benchmarking","howpublished":"","urllink":"","refid":201,"weight":201}
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