// +author:s akbulut +author:akbulut 
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{userid:"m.j.whitcombe", "refid":"77","repocollections":"","attachment":"","_thumb":"","articletype":"article","sectionheading":"","title":"Microplates with adaptive surfaces","year":"2011","author":"M Akbulut, D Lakshmi, M J Whitcombe, E V Piletska, I Chianella, O G\u00fcven, S A Piletsky\r\n","journal":"ACS Combinatorial Science","volume":"13","number":"6","pages":"646-652","month":"","doi":"10.1021\/co200073w","pubmed":"21888414","pdflink":"","urllink":"","abstract":"Here we present a new and versatile method for the modification of the well surfaces of polystyrene micro-titre plates (microplates) with poly(N-phenylethylene diamine methacrylamide), (poly-NPEDMA). The chemical grafting of poly-NPEDMA to the surface of microplates resulted in the formation of thin layers of a polyaniline derivative bearing pendant methacrylamide double bonds. These were used as the attachment point for various functional polymers through photochemical grafting of various, e.g. acrylate and methacrylate, polymers with different functionalities. In a model experiment we have modified poly-NPEDMA-coated microplates with a small library of polymers containing different functional groups using a two-step approach. In the first step, double bonds were activated by UV irradiation in the presence of N,N-diethyldithiocarbamic acid benzyl ester (iniferter). This enabled grafting of the polymer library in the second step by UV irradiation of solutions of the corresponding monomers in the microplate wells. The uniformity of coatings was confirmed spectrophotometrically, by microscopic imaging and by contact angle measurements (CA). The feasibility of the current technology has been shown by the generation of a small library of polymers grafted to the microplate well surfaces and screening of their affinity to small molecules such as atrazine, a trio of organic dyes and a model protein, bovine serum albumin (BSA). The stability of the polymers, reproducibility of measurement, ease of preparation, and cost-effectiveness make this approach suitable for applications in high-throughput screening in the area of materials research.","note":"","tags":"","weight":"77"}
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{userid:"m.j.whitcombe", "refid":"88","repocollections":"","attachment":"","_thumb":"","articletype":"article","sectionheading":"","title":"Computational design and preparation of MIPs for atrazine recognition on a conjugated polymer-coated microtitre plate","year":"2013","author":"D Lakshmi, M Akbulut, P K  Ivanova-Mitseva, M J Whitcombe, E V Piletska, K Karim, O Guven, S A Piletsky","journal":"Industrial & Engineering Chemistry Research","volume":"52","number":"39","pages":"13910-13916","month":"","doi":"10.1021\/ie302982h","pubmed":"","pdflink":"","urllink":"","abstract":"Herein we present a technique for the coating of microplate wells with thin layers of cross-linked polymers, as demonstrated by the preparation of a small library of molecularly imprinted polymers (MIPs) imprinted with atrazine and the corresponding control polymers (NIPs). The composition of MIPs was based on the results of a computational screening using the SYBYL suite of molecular modeling software. The monomers giving the most favorable interaction energies in a virtual monomers screening were: N,N'-methylene-bis-acrylamide, methacrylic acid, N-phenylethylene diamine methacrylamide (NPEDMA) and itaconic acid. The polymer library was prepared by surface grafting to a layer of poly-(NPEDMA), formed by oxidative polymerization in each of the microplate wells, after activation of the methacrylamide double bonds with a photochemical iniferter (benzyl N,N-diethyldithiocarbamate). MIPs and NIPs were prepared with each of the four functional monomers, cross-linked with ethylene glycol dimethacrylate, using template-monomer ratios of 1:1, 1:2 or 1:3. Binding of the template and its structural analogs (simizine and metribuzine) was assessed by LC-MS. The method is shown to be suitable for rapid screening and optimization of MIPs, is capable of automation and uses a simple grafting protocol compatible with conventional MIP compositions. The method not only can be used as a screening tool but could also form the basis of new MIP-based assays","note":"","tags":""}
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