DOI
PMID
sanjibsaha1991@yahoo.com |
Journal articles | |
2013 |
Sanjib Saha, Faroque Hossain, Md Anisuzzman, Md Khirul Islam (2013) Pharmacological evaluation of Musa seminifera
Lour. fruit J Integr Med Abstract: OBJECTIVE: To study potential antioxidant, analgesic, antidiarrheal, and antibacterial activities
of the ethanol extract of Musa seminifera Lour. fruit in different established in vivo and in vitro
experimental models.
METHODS: In vitro antioxidant activity was studied in 2,2-diphenyl-1-picrylhydrazyl (DPPH)
radical-scavenging assay. Phenolic content was determined using Folin-Ciocalteu’s reagent.
Reducing ability was evaluated by ferric reducing power assay. Peripherally and centrally acting
analgesic activity was studied in three different in vivo models, namely, acetic acid-induced
writhing, hot-plate test, and tail-flick test in Swiss albino mice. In vivo antidiarrheal activity was
evaluated in castor oil- and magnesium sulfate-induced diarrhea in mice. Gastrointestinal
motility test was also carried out in mice. All studies in mice were undertaken at the doses of 250
and 500 mg/kg body weight. Antibacterial activity was assessed by disk diffusion assay against
some Gram-positive and Gram-negative bacterial strains. Acute toxicity test was conducted to
assess the safe doses of the extract.
RESULTS: The extract showed 50% inhibitory concentration value of 12.65 μg/mL in DPPH radicalscavenging
assay. Phenolic content was found to be 589.83 mg gallic acid equivalent per 100 g
of dried fruits extract. Reducing power was in a concentration-dependent manner, and strongly
comparable with the standard ascorbic acid. The extract demonstrated significant inhibition
of writhing in acetic acid-induced writhing test at both dose levels (P<0.01). The extract also
raised pain threshold in both hot-plate and tail-flick test in a dose-dependent manner, and the
results were statistically significant (P<0.01). The extract significantly (P<0.01) increased latent
period, and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea.
The extract also decreased gastrointestinal motility in mice. In disk diffusion assay, the extract
showed potential antibacterial activity against all the tested bacterial strains.
CONCLUSION: The results suggest that the ethanol extract of M. seminifera fruit has potential
antioxidant, analgesic, antidiarrheal, and antibacterial activities.
KEYWORDS: Musa; plant extracts; antioxidants; antidiarrheals; analgesics; agents, antibacterial;
mice Notes:
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