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Sabri Musa

Department Of Children's Dentistry And Orthodontics, Faculty of Dentistry Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
sabrim@um.edu.my

Journal articles

2011
Vijayendran Govindasamy, Veronica Sainik Ronald, Aimi Naim Binti Abdullah, Kavitha R Ganesan Nathan, Zeti Adura Che Abdul Aziz, Mariam Abdullah, Rosnah Binti Zain, Noor Hayaty Abu Kasim, Sabri Musa, Ramesh R Bhonde (2011)  Human platelet lysate permits scale-up of dental pulp stromal cells for clinical applications.   Cytotherapy 13: 10. 1221-1233 Nov  
Abstract: BACKGROUND AIMS. Dental pulp stromal cells (DPSC) are considered to be a promising source of stem cells in the field of regenerative therapy. However, the usage of DPSC in transplantation requires large-scale expansion to cater for the need for clinical quantity without compromising current good manufacturing practice (cGMP). Existing protocols for cell culturing make use of fetal bovine serum (FBS) as a nutritional supplement. Unfortunately, FBS is an undesirable additive to cells because it carries the risk of transmitting viral and prion diseases. Therefore, the present study was undertaken to examine the efficacy of human platelet lysate (HPL) as a substitute for FBS in a large-scale set-up. METHODS. We expanded the DPSC in Dulbecco's modified Eagle's medium-knock-out (DMEM-KO) with either 10% FBS or 10% HPL, and studied the characteristics of DPSC at pre- (T25 culture flask) and post- (5-STACK chamber) large-scale expansion in terms of their identity, quality, functionality, molecular signatures and cytogenetic stability. RESULTS. In both pre- and post-large-scale expansion, DPSC expanded in HPL showed extensive proliferation of cells (c. 2-fold) compared with FBS; the purity, immune phenotype, colony-forming unit potential and differentiation were comparable. Furthermore, to understand the gene expression profiling, the transcriptomes and cytogenetics of DPSC expanded under HPL and FBS were compared, revealing similar expression profiles. CONCLUSIONS. We present a highly economized expansion of DPSC in HPL, yielding double the amount of cells while retaining their basic characteristics during a shorter time period under cGMP conditions, making it suitable for therapeutic applications.
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2010
Vijayendran Govindasamy, Aimi Naim Abdullah, Veronica Sainik Ronald, Sabri Musa, Zeti Adura Che Ab Aziz, Rosnah Binti Zain, Satish Totey, Ramesh R Bhonde, Noor Hayaty Abu Kasim (2010)  Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth.   J Endod 36: 9. 1504-1515 Sep  
Abstract: Lately, several new stem cell sources and their effective isolation have been reported that claim to have potential for therapeutic applications. However, it is not yet clear which type of stem cell sources are most potent and best for targeted therapy. Lack of understanding of nature of these cells and their lineage-specific propensity might hinder their full potential. Therefore, understanding the gene expression profile that indicates their lineage-specific proclivity is fundamental to the development of successful cell-based therapies.
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2007
Siti Noor Fazliah Mohd Noor, Sabri Musa (2007)  Assessment of patients' level of satisfaction with cleft treatment using the Cleft Evaluation Profile.   Cleft Palate Craniofac J 44: 3. 292-303 May  
Abstract: OBJECTIVES: Determination of the psychosocial status and assessment of the level of satisfaction in Malaysian cleft palate patients and their parents. DESIGN: Cross-sectional study. PARTICIPANTS AND METHODS: Sixty cleft lip and palate patients (12 to 17 years of age) from Hospital Universiti Sains Malaysia and their parents were selected. The questionnaires used were the Child Interview Schedule, the Parents Interview Schedule, and the Cleft Evaluation Profile (CEP), administered via individual interviews. RESULTS: Patients were teased because of their clefts and felt their self-confidence was affected by the cleft condition. They were frequently teased about cleft-related features such as speech, teeth, and lip appearance. Parents also reported that their children were being teased because of their clefts and that their children's self-confidence was affected by the clefts. Both showed a significant level of satisfaction with the treatment provided by the cleft team. There was no significant difference between the responses of the patients and their parents. The features that were found to be most important for the patients and their parents, in decreasing order of priority, were teeth, nose, lips, and speech. CONCLUSIONS: Cleft lip and/or palate patients were teased because of their clefts, and it affected their self-confidence. The Cleft Evaluation Profile is a reliable and useful tool to assess patients' level of satisfaction with treatment received for cleft lip and/or palate and can identify the types of cleft-related features that are most important for the patients.
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1995
R D Holt, M Wilson, S Musa (1995)  Mycoplasmas in plaque and saliva of children and their relationship to gingivitis.   J Periodontol 66: 2. 97-101 Feb  
Abstract: Gingivitis is common in children but its etiology is not well understood. Results of earlier studies have suggested that mycoplasmas may have a role in the disease. In this study two plaque samples and one stimulated saliva sample were taken from each of a group of sixty (60) 5 to 9 year-old children and cultured selectively for mycoplasmas. Subjects had been selected on the basis of gingival status buccal to the most posterior tooth in an upper quadrant, 20 each having scores 0, 1, or 2 at this site using the Löe and Silness index. Plaque samples were taken from the adjacent tooth surface and from a second posterior site where the adjacent gingiva was free of signs of inflammation. Mycoplasmas were cultured from 54 saliva samples and from 57 plaque samples. Isolation frequency and numbers of mycoplasmas in relation to total anaerobic counts were consistently related to gingivitis scores in both plaque and saliva samples, with higher frequencies and proportions with increasing levels of gingival inflammation at the selected site. In plaque samples this finding held good for both comparisons between groups of subjects and for those between test and control sites within subjects. Findings would appear to confirm the suggestions of earlier studies that mycoplasmas in the oral cavity are associated with gingivitis in children.
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S MUSA, N H ABU KASIM, R B ZAIN, Z A CHE AB AZIZ, A N ABDULLAH, V S RONALD, V GOVINDASAMY, R R BHONDE  Inherent differentiation potential of stem cells from human dental origin    
Abstract: The success of cell replacement therapy depends on the ability of stem cells to stimulate endogenous pool of organ resident stem cells and generating adequate number of cells for therapy. Therefore, understanding the gene expression profile which indicating their lineage specific proclivity is fundamental to the development of successful cell-based therapies. Objectives: In this investigation, we compared the characteristic of stem cells isolated from deciduous (SCD) and permanent teeth (DPSCs) to elucidate their inherent proliferation and differentiation potential. Materials and methods: We compared proliferation rate, gene expression profile and lineage specific propensity of stem cells derived from SCD and DPSCs over five passages. Results: The proliferation rate of SCD was higher (cell number (cells/ml) 25 x 106; percent CFUs: 151.67 ± 10.5; percent cells in S/G2 phase: 12.4 ± 1.48; p<0.05) than DPSCs (cell number (cells/ml) 21 x 106; percent CFUs: 133 ± 17.62; percent cells in S/G2 phase: 10.4 ± 1.18. It was observed that fold expression of several pluripotent markers such as OCT4, SOX2, NANOG and REX1 were higher (> 2) in SCD as compared to DPSCs (p<0.05). However, DPSCs showed higher expression of neuro-ectodermal markers PAX6, GBX2, NESTIN (fold expression >100). Similarly, higher neurospheres formation and neuronal marker expression (NF, GFAP) were found in the differentiated DPSCs into neuron like cells as compared to SCD. Conclusion: This study thus demonstrates that both SCD and DPSCs exhibit specific gene expression profile with clear-cut inclination of DPSCs towards neuronal lineage.
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Govindasamy, V Ronald, V S Abdullah, A N Nathan, K R Ganesan Aziz, Z A C Ab Abdullah, M Musa, S Abu Kasim, R R N H Bhonde  Differentiation of Dental Pulp Stem Cells into Islet-like Aggregates   Journal of Dental Research 90: 5. 646 652  
Abstract: The post-natal dental pulp tissue contains a population of multipotent mesenchymal progenitor cells known as dental pulp stromal/stem cells (DPSCs), with high proliferative potential for self-renewal. In this investigation, we explored the potential of DPSCs to differentiate into pancreatic cell lineage resembling islet-like cell aggregates (ICAs). We isolated, propagated, and characterized DPSCs and demonstrated that these could be differentiated into adipogenic, chondrogenic, and osteogenic lineage upon exposure to an appropriate cocktail of differentiating agents. Using a three-step protocol reported previously by our group, we succeeded in obtaining ICAs from DPSCs. The identity of ICAs was confirmed as islets by dithiozone-positive staining, as well as by expression of C-peptide, Pdx-1, Pax4, Pax6, Ngn3, and Isl-1. There were several-fold up-regulations of these transcription factors proportional to days of differentiation as compared with undifferentiated DPSCs. Day 10 ICAs released insulin and C-peptide in a glucose-dependent manner, exhibiting in vitro functionality. Our results demonstrated for the first time that DPSCs could be differentiated into pancreatic cell lineage and offer an unconventional and non-controversial source of human tissue that could be used for autologous stem cell therapy in diabetes.
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